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Protein

F-box only protein 6

Gene

Fbxo6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Substrate-recognition component of some SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complexes. Involved in endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranlocated into the cytosol and promoting their ubiquitination and subsequent degradation. Able to recognize and bind denatured glycoproteins, which are modified with not only high-mannose but also complex-type oligosaccharides. Also recognizes sulfated glycans. Also involved in DNA damage response by specifically recognizing activated CHEK1 (phosphorylated on 'Ser-345'), promoting its ubiquitination and degradation. Ubiquitination of CHEK1 is required to insure that activated CHEK1 does not accumulate as cells progress through S phase, or when replication forks encounter transient impediments during normal DNA replication (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway, Unfolded protein response

Enzyme and pathway databases

ReactomeiR-RNO-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box only protein 6
Alternative name(s):
F-box only protein 6b
F-box protein that recognizes sugar chains 2
F-box/G-domain protein 2
Gene namesi
Name:Fbxo6
Synonyms:Fbg2, Fbs2, Fbxo6b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi620418. Fbxo6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 284284F-box only protein 6PRO_0000119884Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei268 – 2681PhosphoserineBy similarity
Modified residuei275 – 2751PhosphoserineCombined sources
Modified residuei278 – 2781PhosphoserineCombined sources
Modified residuei283 – 2831PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ923V4.
PRIDEiQ923V4.

PTM databases

iPTMnetiQ923V4.
PhosphoSiteiQ923V4.

Expressioni

Developmental stagei

Abundantly expressed in 14.5-day fetal liver. Expression decreases during late liver development and stays at low levels until birth. Expression increases 24 hours after birth and reaches highest levels in adult liver.1 Publication

Gene expression databases

GenevisibleiQ923V4. RN.

Interactioni

Subunit structurei

Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with VCP, CHEK1 and CUL1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012301.

Structurei

3D structure databases

ProteinModelPortaliQ923V4.
SMRiQ923V4. Positions 68-249.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 4848F-boxPROSITE-ProRule annotationAdd
BLAST
Domaini69 – 250182FBAPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 F-box domain.PROSITE-ProRule annotation
Contains 1 FBA (F-box associated) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IK6V. Eukaryota.
ENOG4111MF5. LUCA.
GeneTreeiENSGT00390000003865.
HOGENOMiHOG000231084.
HOVERGENiHBG003593.
InParanoidiQ923V4.
KOiK10100.
OMAiEMCLKSQ.
OrthoDBiEOG7DRJ3R.
PhylomeDBiQ923V4.
TreeFamiTF320527.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
InterProiIPR007397. F-box-assoc_dom.
IPR001810. F-box_dom.
IPR008979. Galactose-bd-like.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF04300. FBA. 1 hit.
[Graphical view]
SMARTiSM01198. FBA. 1 hit.
SM00256. FBOX. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS51114. FBA. 1 hit.
PS50181. FBOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q923V4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNINELPEN ILLELFTHVP APQLLRNCRL VCSLWRDLID VMTLWKRKSL
60 70 80 90 100
REGFVTKDRD EPVDDWKIFY ILCSLQRNLL RNPCAEENLR SWRIDSNGGD
110 120 130 140 150
EWKVESLPGD HGTSFPDTKV KKYFVTSYGM CLKSQMVDLK AEGYSEKLLD
160 170 180 190 200
TVRPDIVVKD WFAPRADCGC TYHLRVQLAS ADYIVLASFE PPPVTIEQWN
210 220 230 240 250
DASWQEISHT FSNYPPGVRH ILFQHGGKDT QFWKGWYGPR VTNSSIIVSH
260 270 280
RTAKNPAPAR TLPEEDTSNR RKILSFGSWE DLSP
Length:284
Mass (Da):32,786
Last modified:December 1, 2001 - v1
Checksum:i75BFF3C69E676943
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti147 – 1471K → E in AAH63148 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF393484 mRNA. Translation: AAK70899.1.
BC063148 mRNA. Translation: AAH63148.1.
RefSeqiNP_620272.3. NM_138917.3.
XP_006239434.1. XM_006239372.2.
XP_006239435.1. XM_006239373.2.
XP_006239436.1. XM_006239374.2.
XP_006239437.1. XM_006239375.2.
UniGeneiRn.57325.

Genome annotation databases

EnsembliENSRNOT00000012301; ENSRNOP00000012301; ENSRNOG00000009217.
GeneIDi192351.
KEGGirno:192351.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF393484 mRNA. Translation: AAK70899.1.
BC063148 mRNA. Translation: AAH63148.1.
RefSeqiNP_620272.3. NM_138917.3.
XP_006239434.1. XM_006239372.2.
XP_006239435.1. XM_006239373.2.
XP_006239436.1. XM_006239374.2.
XP_006239437.1. XM_006239375.2.
UniGeneiRn.57325.

3D structure databases

ProteinModelPortaliQ923V4.
SMRiQ923V4. Positions 68-249.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012301.

PTM databases

iPTMnetiQ923V4.
PhosphoSiteiQ923V4.

Proteomic databases

PaxDbiQ923V4.
PRIDEiQ923V4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012301; ENSRNOP00000012301; ENSRNOG00000009217.
GeneIDi192351.
KEGGirno:192351.

Organism-specific databases

CTDi26270.
RGDi620418. Fbxo6.

Phylogenomic databases

eggNOGiENOG410IK6V. Eukaryota.
ENOG4111MF5. LUCA.
GeneTreeiENSGT00390000003865.
HOGENOMiHOG000231084.
HOVERGENiHBG003593.
InParanoidiQ923V4.
KOiK10100.
OMAiEMCLKSQ.
OrthoDBiEOG7DRJ3R.
PhylomeDBiQ923V4.
TreeFamiTF320527.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-RNO-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

NextBioi623025.
PROiQ923V4.

Gene expression databases

GenevisibleiQ923V4. RN.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
InterProiIPR007397. F-box-assoc_dom.
IPR001810. F-box_dom.
IPR008979. Galactose-bd-like.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF04300. FBA. 1 hit.
[Graphical view]
SMARTiSM01198. FBA. 1 hit.
SM00256. FBOX. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF81383. SSF81383. 1 hit.
PROSITEiPS51114. FBA. 1 hit.
PS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A new subfamily of structurally related human F-box proteins."
    Ilyin G.P., Serandour A.L., Pigeon C., Rialland M., Glaise D., Guguen-Guillouzo C.
    Gene 296:11-20(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Strain: Sprague-Dawley.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-275; SER-278 AND SER-283, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFBX6_RAT
AccessioniPrimary (citable) accession number: Q923V4
Secondary accession number(s): Q6P512
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: December 1, 2001
Last modified: May 11, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.