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Reviewed, UniProtKB/Swiss-Prot Q923T9 (KCC2G_MOUSE)

Last modified February 9, 2010. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Calcium/calmodulin-dependent protein kinase type II subunit gamma
      Short name=CaM kinase II subunit gamma
      Short name=CaMK-II subunit gamma
    EC=2.7.11.17
Gene names
Name: Camk2g
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length529 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Autophosphorylation of CAMK2 plays an important role in the regulation of the kinase activity.

Subunit structure

CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

Contains 1 protein kinase domain.

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q923T9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q923T9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     331-341: Missing.
Isoform 3 (identifier: Q923T9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     331-364: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 529529Calcium/calmodulin-dependent protein kinase type II subunit gamma
PRO_0000086102

Regions

Domain14 – 272259Protein kinase
Nucleotide binding20 – 289ATP By similarity
Region291 – 30111Calmodulin-binding By similarity

Sites

Active site1361Proton acceptor By similarity
Binding site431ATP By similarity

Amino acid modifications

Modified residue2351Phosphoserine Ref.5
Modified residue2871Phosphothreonine Ref.5 Ref.4
Modified residue3071Phosphothreonine By similarity
Modified residue3111Phosphoserine By similarity
Modified residue3151Phosphoserine By similarity
Modified residue3191Phosphoserine By similarity
Modified residue3251Phosphoserine By similarity
Modified residue3491Phosphoserine By similarity
Modified residue3521Phosphoserine By similarity
Modified residue3751Phosphothreonine By similarity
Modified residue3811Phosphoserine By similarity
Modified residue3821Phosphothreonine By similarity
Modified residue3841Phosphoserine By similarity
Modified residue3871Phosphothreonine By similarity
Modified residue3881Phosphothreonine By similarity

Natural variations

Alternative sequence331 – 36434Missing in isoform 3.
VSP_004780
Alternative sequence331 – 34111Missing in isoform 2.
VSP_004779

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: EE1D127BBA178544

FASTA52959,607
        10         20         30         40         50         60 
MATTATCTRF TDDYQLFEEL GKGAFSVVRR CVKKTSTQEY AAKIINTKKL SARDHQKLER 

        70         80         90        100        110        120 
EARICRLLKH PNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIHQI 

       130        140        150        160        170        180 
LESVNHIHQH DIVHRDLKPE NLLLASKCKG AAVKLADFGL AIEVQGEQQA WFGFAGTPGY 

       190        200        210        220        230        240 
LSPEVLRKDP YGKPVDIWAC GVILYILLVG YPPFWDEDQH KLYQQIKAGA YDFPSPEWDT 

       250        260        270        280        290        300 
VTPEAKNLIN QMLTINPAKR ITADQALKHP WVCQRSTVAS MMHRQETVEC LRKFNARRKL 

       310        320        330        340        350        360 
KGAILTTMLV SRNFSAAKSL LNKKSDGGVK KRKSSSSVHL MPQSNNKNSL VSPAQEPAPL 

       370        380        390        400        410        420 
QTAMEPQTTV VHNATDGIKG STESCNTTTE DEDLKVRKQE IIKITEQLIE AINNGDFEAY 

       430        440        450        460        470        480 
TKICDPGLTS FEPEALGNLV EGMDFHKFYF ENLLSKNSKP IHTTILNPHV HVIGEDAACI 

       490        500        510        520 
AYIRLTQYID GQGRPRTSQS EETRVWHRRD GKWLNVHYHC SGAPAAPLQ

« Hide

Isoform 2.

Checksum: 127250AF541196BF
Show »

FASTA51858,365
Isoform 3.

Checksum: 168EA409723DF4C4
Show »

FASTA49555,961

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References

« Hide 'large scale' references
[1]"Identification of a mouse Ca2+/calmodulin-dependent protein kinase II."
Szendro P.I., Cadenas C., Eichele G.
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: C57BL/6.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: NOD.
Tissue: Dendritic cell.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
[4]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed: 16452087] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-287, MASS SPECTROMETRY.
Tissue: Brain.
[5]"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-235 AND THR-287, MASS SPECTROMETRY.
Tissue: Brain cortex.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF395884 mRNA. Translation: AAK84142.1.
AK154764 mRNA. Translation: BAE32813.1.
BC019162 mRNA. Translation: AAH19162.1.
BC025597 mRNA. Translation: AAH25597.1.
IPIIPI00124695.
IPI00228044.
IPI00228045.
RefSeqNP_001034227.1.
NP_001034228.1.
NP_848712.2.
UniGeneMm.235182

3D structure databases

SMRQ923T9. Positions 3-302, 389-523.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ923T9.

PTM databases

PhosphoSiteQ923T9.

Proteomic databases

PRIDEQ923T9.

Genome annotation databases

EnsemblENSMUST00000071816; ENSMUSP00000071720; ENSMUSG00000021820; Mus musculus. [Genome view]
ENSMUST00000080440; ENSMUSP00000079298; ENSMUSG00000021820; Mus musculus. [Genome view]
ENSMUST00000100837; ENSMUSP00000098398; ENSMUSG00000021820; Mus musculus. [Genome view]
GeneID12325.
KEGGmmu:12325.
UCSCuc007skt.1. mouse.
uc007sku.1. mouse.
uc007skv.1. mouse.

Organism-specific databases

CTD12325.
MGIMGI:88259. Camk2g.

Phylogenomic databases

eggNOGroNOG13785.
HOGENOMHBG755340.
HOVERGENQ923T9.
InParanoidQ923T9.
OMAXPQSNNK.
PhylomeDBQ923T9.

Enzyme and pathway databases

BRENDA2.7.11.17. 244.

Gene expression databases

ArrayExpressQ923T9.
BgeeQ923T9.
GenevestigatorQ923T9.
GermOnlineENSMUSG00000021820. Mus musculus.

Family and domain databases

InterProIPR020636. Ca/CaM-dep_prot_kinase-like.
IPR015742. Ca/CaM-dep_prot_kinase_2.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_prot_kinase-like_dom.
IPR008271. Ser/Thr_prot_kinase_AS.
IPR002290. Ser/Thr_prot_kinase_dom.
[Graphical view]
PANTHERPTHR22982. Ca/CaM-dep_prot_kinase-like. 1 hit.
PTHR22982:SF64. CaMKII. 1 hit.
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio280908.
SOURCESearch...

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Entry information

Entry nameKCC2G_MOUSE
AccessionPrimary (citable) accession number: Q923T9
Secondary accession number(s): Q3U3H3, Q8VED3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: December 1, 2001
Last modified: February 9, 2010
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents