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Protein

tRNA modification GTPase GTPBP3, mitochondrial

Gene

Gtpbp3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

GTPase involved in the 5-carboxymethylaminomethyl modification (mnm5s2U34) of the wobble uridine base in mitochondrial tRNAs.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi256 – 2638GTPSequence analysis
Nucleotide bindingi303 – 3075GTPSequence analysis
Nucleotide bindingi374 – 3774GTPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA modification GTPase GTPBP3, mitochondrial
Alternative name(s):
GTP-binding protein 3
Gene namesi
Name:Gtpbp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1917609. Gtpbp3.

Subcellular locationi

  • Mitochondrion 1 Publication

GO - Cellular componenti

  • mitochondrion Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 8181MitochondrionSequence analysisAdd
BLAST
Chaini82 – 492411tRNA modification GTPase GTPBP3, mitochondrialPRO_0000280266Add
BLAST

Proteomic databases

EPDiQ923K4.
MaxQBiQ923K4.
PaxDbiQ923K4.
PRIDEiQ923K4.

PTM databases

PhosphoSiteiQ923K4.

Expressioni

Tissue specificityi

Ubiquitously expressed. Highly expressed in tissues with high metabolic rates including heart, liver and brain. Weakly expressed in skeletal muscle.1 Publication

Gene expression databases

BgeeiQ923K4.
CleanExiMM_GTPBP3.
ExpressionAtlasiQ923K4. baseline and differential.
GenevisibleiQ923K4. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000007754.

Structurei

3D structure databases

ProteinModelPortaliQ923K4.
SMRiQ923K4. Positions 35-492.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini249 – 416168TrmE-type GAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1191. Eukaryota.
COG0486. LUCA.
GeneTreeiENSGT00390000016851.
HOGENOMiHOG000200714.
HOVERGENiHBG081577.
InParanoidiQ923K4.
KOiK03650.
OrthoDBiEOG7PS1FF.
PhylomeDBiQ923K4.
TreeFamiTF313153.

Family and domain databases

Gene3Di1.20.120.430. 3 hits.
3.30.1360.120. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00379. GTPase_MnmE.
InterProiIPR031168. G_TrmE.
IPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_MnmE.
IPR027368. MnmE_dom2.
IPR025867. MnmE_helical.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
PF12631. MnmE_helical. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51709. G_TRME. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q923K4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWRGLSALVT QAAWAPLRLC ARCSTSAESL VPSSTIFALS SGQGRCAIAV
60 70 80 90 100
IRTSGPASGL ALRSLTALQE PPPARRACLR LLRHPCSGEP LDRSLVLWFP
110 120 130 140 150
GPQSFTGEDC VEFHVHGGPA VVSGVLQALG SVPGLRPAEA GEFTRRAFAH
160 170 180 190 200
GKLSLTEVEG LADLIRAETE AQRRQALRQL DGELSQLCQG WAKTLTKALA
210 220 230 240 250
YVEAYIDFGE DDNLEEGVLE QADREVRALE VALGSHLRDA RRGQRLLSGA
260 270 280 290 300
NVVVTGPPNA GKSSLVNLLS QKPVSIVSPE PGTTRDVLET PVDLAGFPVL
310 320 330 340 350
LSDTAGLREG VGAVEQEGVR RARHRLEQAD IILGVLDASD LASSSSCSFL
360 370 380 390 400
DTVVTPLLAQ SQDSGGQRLL LLLNKSDLLS ANAPACDIAL PPHLLLSCHT
410 420 430 440 450
GAGMDSLLQA LKTELAAVCG DPSTGPPLLT RVRHQYHLQG CLDALGHYQL
460 470 480 490
ATDLALAAEA LRQARRQLNH LTGGGGTEEI LDLIFQDFCV GK
Length:492
Mass (Da):52,175
Last modified:December 1, 2001 - v1
Checksum:i85BB8A5E38F93597
GO
Isoform 2 (identifier: Q923K4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     198-241: ALAYVEAYID...ALGSHLRDAR → RTEKFEPWRW...DLPMRARAVW
     242-492: Missing.

Note: No experimental confirmation available.
Show »
Length:241
Mass (Da):26,324
Checksum:i05ECB4C9CBBE30B3
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei198 – 24144ALAYV…LRDAR → RTEKFEPWRWRWVPTCEMLG VDKGSSRGQMLWSLDLPMRA RAVW in isoform 2. 1 PublicationVSP_023585Add
BLAST
Alternative sequencei242 – 492251Missing in isoform 2. 1 PublicationVSP_023586Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF361482 mRNA. Translation: AAK35216.2.
AY029613 Genomic DNA. Translation: AAK60410.1.
BC092042 mRNA. Translation: AAH92042.1.
CCDSiCCDS40384.1. [Q923K4-1]
RefSeqiNP_115933.2. NM_032544.3. [Q923K4-1]
XP_006544816.1. XM_006544753.2. [Q923K4-1]
UniGeneiMm.142112.

Genome annotation databases

EnsembliENSMUST00000007754; ENSMUSP00000007754; ENSMUSG00000007610. [Q923K4-1]
ENSMUST00000095259; ENSMUSP00000092892; ENSMUSG00000007610. [Q923K4-2]
ENSMUST00000150969; ENSMUSP00000114193; ENSMUSG00000007610. [Q923K4-1]
GeneIDi102642476.
70359.
KEGGimmu:102642476.
mmu:70359.
UCSCiuc009mdn.1. mouse. [Q923K4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF361482 mRNA. Translation: AAK35216.2.
AY029613 Genomic DNA. Translation: AAK60410.1.
BC092042 mRNA. Translation: AAH92042.1.
CCDSiCCDS40384.1. [Q923K4-1]
RefSeqiNP_115933.2. NM_032544.3. [Q923K4-1]
XP_006544816.1. XM_006544753.2. [Q923K4-1]
UniGeneiMm.142112.

3D structure databases

ProteinModelPortaliQ923K4.
SMRiQ923K4. Positions 35-492.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000007754.

PTM databases

PhosphoSiteiQ923K4.

Proteomic databases

EPDiQ923K4.
MaxQBiQ923K4.
PaxDbiQ923K4.
PRIDEiQ923K4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000007754; ENSMUSP00000007754; ENSMUSG00000007610. [Q923K4-1]
ENSMUST00000095259; ENSMUSP00000092892; ENSMUSG00000007610. [Q923K4-2]
ENSMUST00000150969; ENSMUSP00000114193; ENSMUSG00000007610. [Q923K4-1]
GeneIDi102642476.
70359.
KEGGimmu:102642476.
mmu:70359.
UCSCiuc009mdn.1. mouse. [Q923K4-1]

Organism-specific databases

CTDi84705.
MGIiMGI:1917609. Gtpbp3.

Phylogenomic databases

eggNOGiKOG1191. Eukaryota.
COG0486. LUCA.
GeneTreeiENSGT00390000016851.
HOGENOMiHOG000200714.
HOVERGENiHBG081577.
InParanoidiQ923K4.
KOiK03650.
OrthoDBiEOG7PS1FF.
PhylomeDBiQ923K4.
TreeFamiTF313153.

Miscellaneous databases

PROiQ923K4.
SOURCEiSearch...

Gene expression databases

BgeeiQ923K4.
CleanExiMM_GTPBP3.
ExpressionAtlasiQ923K4. baseline and differential.
GenevisibleiQ923K4. MM.

Family and domain databases

Gene3Di1.20.120.430. 3 hits.
3.30.1360.120. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00379. GTPase_MnmE.
InterProiIPR031168. G_TrmE.
IPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_MnmE.
IPR027368. MnmE_dom2.
IPR025867. MnmE_helical.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
PF12631. MnmE_helical. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51709. G_TRME. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of mouse GTPBP3 gene encoding a mitochondrial GTP-binding protein involved in tRNA modification."
    Li X., Guan M.-X.
    Biochem. Biophys. Res. Commun. 312:747-754(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: FVB/N-3.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiGTPB3_MOUSE
AccessioniPrimary (citable) accession number: Q923K4
Secondary accession number(s): Q58E73, Q99M99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: December 1, 2001
Last modified: June 8, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.