Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein Spindly

Gene

Spdl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2500257. Resolution of Sister Chromatid Cohesion.
R-MMU-5663220. RHO GTPases Activate Formins.
R-MMU-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SpindlyUniRule annotation
Alternative name(s):
Coiled-coil domain-containing protein 99UniRule annotation
Spindle apparatus coiled-coil domain-containing protein 1UniRule annotation
Gene namesi
Name:Spdl1
Synonyms:Ccdc99
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1917635. Spdl1.

Subcellular locationi

  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome UniRule annotation
  • Chromosomecentromerekinetochore UniRule annotation
  • Nucleus
  • Cytoplasmcytoskeletonspindle pole UniRule annotation

  • Note: Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002745181 – 608Protein SpindlyAdd BLAST608

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei516PhosphoserineBy similarity1
Modified residuei518PhosphoserineBy similarity1
Modified residuei558PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ923A2.
MaxQBiQ923A2.
PaxDbiQ923A2.
PeptideAtlasiQ923A2.
PRIDEiQ923A2.

PTM databases

iPTMnetiQ923A2.
PhosphoSitePlusiQ923A2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000069910.
CleanExiMM_CCDC99.
GenevisibleiQ923A2. MM.

Interactioni

Subunit structurei

Interacts with KNTC1 and ZW10. These interactions appear weak and may be transient or indirect.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000090882.

Structurei

3D structure databases

ProteinModelPortaliQ923A2.
SMRiQ923A2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1 – 445UniRule annotationAdd BLAST445

Sequence similaritiesi

Belongs to the Spindly family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IDXF. Eukaryota.
ENOG4110EUG. LUCA.
GeneTreeiENSGT00510000047951.
HOGENOMiHOG000070239.
InParanoidiQ923A2.
OMAiCECEAIK.
OrthoDBiEOG091G08GL.
PhylomeDBiQ923A2.
TreeFamiTF332470.

Family and domain databases

HAMAPiMF_03041. SPDLY. 1 hit.
InterProiIPR028593. SPDLY_chordates.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q923A2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEADITNLRN KLKECEDERL KAAHYGLQLL ERQTELQSQL DKCHEEMMIT
60 70 80 90 100
AEKYNQEKHA LQREVELKSR MLDSLSCECE ALKQQQKAQL EQLEVQLHRS
110 120 130 140 150
HRQEVSDLKN KLENLKVELD EARLGEKQLK QKLDLQGELL AHKSEELRLL
160 170 180 190 200
SEQRVLSSMS SELLALETEL TAAEGVKNAL KEEVNELQYK QEQLECLNTS
210 220 230 240 250
LLHQVDRLKE EKEEREREAV SYYNALEKAR VENQDLQVQL GHALQQAADP
260 270 280 290 300
NSKGNSLFAE VEDRRVAMER QLNLMKDKYQ SLKKQNAFTR DQMNKMKLQI
310 320 330 340 350
STLLRMRGSQ TEFEQQERLF AMIEQKNGEI KHLLGEINKL EKFKNLYESM
360 370 380 390 400
ESRPSTSDTA CVLEDSTYYS DLLQLKLDKL NKENESTKDE LSIQRMKALF
410 420 430 440 450
ESQRALDIER KLFTNERHLQ LSESENMKLR AKLDELKLKY EPEERIEVPV
460 470 480 490 500
LKRRREVLPL NITTPEETEE TAAASATEDG VSRLPPHREE ESCLNSLKDN
510 520 530 540 550
TVQWKQPASS CVQPASLSPH KNLHLDTQPK KEKKCVKLVD SPANIEVLHE
560 570 580 590 600
QSGNTPNSPR LTAESKLPTE VKERIETTSK LGKGACKKSH NIIYVSSKSA

PETQCSQQ
Length:608
Mass (Da):70,237
Last modified:February 6, 2007 - v2
Checksum:i22906AAABE155352
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti167E → Q in AAH06674 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK139553 mRNA. Translation: BAE24059.1.
CR392356 Genomic DNA. Translation: CAI51911.1.
BC006674 mRNA. Translation: AAH06674.1.
CCDSiCCDS24541.1.
RefSeqiNP_081687.2. NM_027411.2.
XP_006534232.1. XM_006534169.3.
UniGeneiMm.194368.

Genome annotation databases

EnsembliENSMUST00000093191; ENSMUSP00000090882; ENSMUSG00000069910.
GeneIDi70385.
KEGGimmu:70385.
UCSCiuc007ilc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK139553 mRNA. Translation: BAE24059.1.
CR392356 Genomic DNA. Translation: CAI51911.1.
BC006674 mRNA. Translation: AAH06674.1.
CCDSiCCDS24541.1.
RefSeqiNP_081687.2. NM_027411.2.
XP_006534232.1. XM_006534169.3.
UniGeneiMm.194368.

3D structure databases

ProteinModelPortaliQ923A2.
SMRiQ923A2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000090882.

PTM databases

iPTMnetiQ923A2.
PhosphoSitePlusiQ923A2.

Proteomic databases

EPDiQ923A2.
MaxQBiQ923A2.
PaxDbiQ923A2.
PeptideAtlasiQ923A2.
PRIDEiQ923A2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000093191; ENSMUSP00000090882; ENSMUSG00000069910.
GeneIDi70385.
KEGGimmu:70385.
UCSCiuc007ilc.2. mouse.

Organism-specific databases

CTDi54908.
MGIiMGI:1917635. Spdl1.

Phylogenomic databases

eggNOGiENOG410IDXF. Eukaryota.
ENOG4110EUG. LUCA.
GeneTreeiENSGT00510000047951.
HOGENOMiHOG000070239.
InParanoidiQ923A2.
OMAiCECEAIK.
OrthoDBiEOG091G08GL.
PhylomeDBiQ923A2.
TreeFamiTF332470.

Enzyme and pathway databases

ReactomeiR-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2500257. Resolution of Sister Chromatid Cohesion.
R-MMU-5663220. RHO GTPases Activate Formins.
R-MMU-68877. Mitotic Prometaphase.

Miscellaneous databases

PROiQ923A2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000069910.
CleanExiMM_CCDC99.
GenevisibleiQ923A2. MM.

Family and domain databases

HAMAPiMF_03041. SPDLY. 1 hit.
InterProiIPR028593. SPDLY_chordates.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPDLY_MOUSE
AccessioniPrimary (citable) accession number: Q923A2
Secondary accession number(s): Q5DY45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: February 6, 2007
Last modified: November 2, 2016
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.