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Protein

Protein Spindly

Gene

Spdl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (By similarity).UniRule annotationBy similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SpindlyUniRule annotation
Alternative name(s):
Coiled-coil domain-containing protein 99UniRule annotation
Spindle apparatus coiled-coil domain-containing protein 1UniRule annotation
Gene namesi
Name:Spdl1
Synonyms:Ccdc99
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1917635 Spdl1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002745181 – 608Protein SpindlyAdd BLAST608

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei516PhosphoserineBy similarity1
Modified residuei518PhosphoserineBy similarity1
Modified residuei558PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ923A2
MaxQBiQ923A2
PaxDbiQ923A2
PeptideAtlasiQ923A2
PRIDEiQ923A2

PTM databases

iPTMnetiQ923A2
PhosphoSitePlusiQ923A2

Expressioni

Gene expression databases

BgeeiENSMUSG00000069910
CleanExiMM_CCDC99
GenevisibleiQ923A2 MM

Interactioni

Subunit structurei

Interacts with KNTC1 and ZW10. These interactions appear weak and may be transient or indirect. Interacts with dynein intermediate chain and dynactin (DCTN1) (By similarity).UniRule annotationBy similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000090882

Structurei

3D structure databases

ProteinModelPortaliQ923A2
SMRiQ923A2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1 – 445UniRule annotationAdd BLAST445

Sequence similaritiesi

Belongs to the Spindly family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IDXF Eukaryota
ENOG4110EUG LUCA
GeneTreeiENSGT00510000047951
HOGENOMiHOG000070239
InParanoidiQ923A2
OMAiCECEAIK
OrthoDBiEOG091G08GL
PhylomeDBiQ923A2
TreeFamiTF332470

Family and domain databases

HAMAPiMF_03041 SPDLY, 1 hit
InterProiView protein in InterPro
IPR028593 SPDLY_chordates

Sequencei

Sequence statusi: Complete.

Q923A2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEADITNLRN KLKECEDERL KAAHYGLQLL ERQTELQSQL DKCHEEMMIT
60 70 80 90 100
AEKYNQEKHA LQREVELKSR MLDSLSCECE ALKQQQKAQL EQLEVQLHRS
110 120 130 140 150
HRQEVSDLKN KLENLKVELD EARLGEKQLK QKLDLQGELL AHKSEELRLL
160 170 180 190 200
SEQRVLSSMS SELLALETEL TAAEGVKNAL KEEVNELQYK QEQLECLNTS
210 220 230 240 250
LLHQVDRLKE EKEEREREAV SYYNALEKAR VENQDLQVQL GHALQQAADP
260 270 280 290 300
NSKGNSLFAE VEDRRVAMER QLNLMKDKYQ SLKKQNAFTR DQMNKMKLQI
310 320 330 340 350
STLLRMRGSQ TEFEQQERLF AMIEQKNGEI KHLLGEINKL EKFKNLYESM
360 370 380 390 400
ESRPSTSDTA CVLEDSTYYS DLLQLKLDKL NKENESTKDE LSIQRMKALF
410 420 430 440 450
ESQRALDIER KLFTNERHLQ LSESENMKLR AKLDELKLKY EPEERIEVPV
460 470 480 490 500
LKRRREVLPL NITTPEETEE TAAASATEDG VSRLPPHREE ESCLNSLKDN
510 520 530 540 550
TVQWKQPASS CVQPASLSPH KNLHLDTQPK KEKKCVKLVD SPANIEVLHE
560 570 580 590 600
QSGNTPNSPR LTAESKLPTE VKERIETTSK LGKGACKKSH NIIYVSSKSA

PETQCSQQ
Length:608
Mass (Da):70,237
Last modified:February 6, 2007 - v2
Checksum:i22906AAABE155352
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti167E → Q in AAH06674 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK139553 mRNA Translation: BAE24059.1
CR392356 Genomic DNA No translation available.
BC006674 mRNA Translation: AAH06674.1
CCDSiCCDS24541.1
RefSeqiNP_081687.2, NM_027411.2
XP_006534232.1, XM_006534169.3
UniGeneiMm.194368

Genome annotation databases

EnsembliENSMUST00000093191; ENSMUSP00000090882; ENSMUSG00000069910
GeneIDi70385
KEGGimmu:70385
UCSCiuc007ilc.2 mouse

Similar proteinsi

Entry informationi

Entry nameiSPDLY_MOUSE
AccessioniPrimary (citable) accession number: Q923A2
Secondary accession number(s): Q5DY45
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: February 6, 2007
Last modified: January 31, 2018
This is version 96 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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