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Protein

DNA-directed RNA polymerases I, II, and III subunit RPABC3

Gene

rpb8

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.

GO - Molecular functioni

GO - Biological processi

  • transcription from RNA polymerase III promoter Source: PomBase
  • transcription from RNA polymerase II promoter Source: PomBase
  • transcription from RNA polymerase I promoter Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Transcription

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-SPO-113418. Formation of the Early Elongation Complex.
R-SPO-1834949. Cytosolic sensors of pathogen-associated DNA.
R-SPO-674695. RNA Polymerase II Pre-transcription Events.
R-SPO-6781823. Formation of TC-NER Pre-Incision Complex.
R-SPO-6782135. Dual incision in TC-NER.
R-SPO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SPO-72086. mRNA Capping.
R-SPO-72165. mRNA Splicing - Minor Pathway.
R-SPO-73762. RNA Polymerase I Transcription Initiation.
R-SPO-73776. RNA Polymerase II Promoter Escape.
R-SPO-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SPO-75953. RNA Polymerase II Transcription Initiation.
R-SPO-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SPO-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerases I, II, and III subunit RPABC3
Short name:
RNA polymerases I, II, and III subunit ABC3
Alternative name(s):
DNA-directed RNA polymerases I, II, and III 14.5 kDa polypeptide
RPC14
Gene namesi
Name:rpb8
ORF Names:SPBC14C8.12
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC14C8.12.
PomBaseiSPBC14C8.12. rpb8.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • DNA-directed RNA polymerase I complex Source: PomBase
  • DNA-directed RNA polymerase II, core complex Source: PomBase
  • DNA-directed RNA polymerase II, holoenzyme Source: PomBase
  • DNA-directed RNA polymerase III complex Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 125125DNA-directed RNA polymerases I, II, and III subunit RPABC3PRO_0000074000Add
BLAST

Proteomic databases

MaxQBiQ92399.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of 14, 12 and 17 subunits, respectively. Directly interacts with POLR2A (By similarity).By similarity

Protein-protein interaction databases

BioGridi276344. 10 interactions.
IntActiQ92399. 1 interaction.
MINTiMINT-1214018.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H0GX-ray3.65H/T1-125[»]
ProteinModelPortaliQ92399.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ92399.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni17 – 3923Non-specific ssDNA bindingBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000175572.
InParanoidiQ92399.
KOiK03016.
OMAiYVCHGKI.
OrthoDBiEOG78D7XD.
PhylomeDBiQ92399.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
InterProiIPR012340. NA-bd_OB-fold.
IPR005570. RNA_pol_Rpb8.
[Graphical view]
PANTHERiPTHR10917. PTHR10917. 1 hit.
PfamiPF03870. RNA_pol_Rpb8. 1 hit.
[Graphical view]
SMARTiSM00658. RPOL8c. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.

Sequencei

Sequence statusi: Complete.

Q92399-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSESVLLDEI FTVTSVDKQK YQRVSRITAV SGQNDMNLTL DINSQIYPLE
60 70 80 90 100
KDATFSLQIT SNLNSPDLKE AADYIMYGKV YRVEEAKDEK VSVYVSFGGL
110 120
LMAIEGSHRK LYRLSLDHVY LLLRR
Length:125
Mass (Da):14,300
Last modified:July 15, 1998 - v2
Checksum:i8941D1BB8D624770
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti122 – 1254LLRR → YLEGK in CAA68926 (PubMed:9927738).Curated
Sequence conflicti122 – 1254LLRR → YLEGK in CAA68927 (PubMed:9927738).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89595 Genomic DNA. Translation: BAA34367.1.
D86555 mRNA. Translation: BAA22803.1.
AF020780 mRNA. Translation: AAB93482.1.
AF029688 Genomic DNA. Translation: AAC39321.1.
Y07643 mRNA. Translation: CAA68926.1.
Y07644 mRNA. Translation: CAA68927.1.
CU329671 Genomic DNA. Translation: CAA18430.1.
PIRiT43540.
T45232.
RefSeqiNP_595915.1. NM_001021823.2.

Genome annotation databases

EnsemblFungiiSPBC14C8.12.1; SPBC14C8.12.1:pep; SPBC14C8.12.
GeneIDi2539794.
KEGGispo:SPBC14C8.12.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89595 Genomic DNA. Translation: BAA34367.1.
D86555 mRNA. Translation: BAA22803.1.
AF020780 mRNA. Translation: AAB93482.1.
AF029688 Genomic DNA. Translation: AAC39321.1.
Y07643 mRNA. Translation: CAA68926.1.
Y07644 mRNA. Translation: CAA68927.1.
CU329671 Genomic DNA. Translation: CAA18430.1.
PIRiT43540.
T45232.
RefSeqiNP_595915.1. NM_001021823.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H0GX-ray3.65H/T1-125[»]
ProteinModelPortaliQ92399.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276344. 10 interactions.
IntActiQ92399. 1 interaction.
MINTiMINT-1214018.

Proteomic databases

MaxQBiQ92399.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC14C8.12.1; SPBC14C8.12.1:pep; SPBC14C8.12.
GeneIDi2539794.
KEGGispo:SPBC14C8.12.

Organism-specific databases

EuPathDBiFungiDB:SPBC14C8.12.
PomBaseiSPBC14C8.12. rpb8.

Phylogenomic databases

HOGENOMiHOG000175572.
InParanoidiQ92399.
KOiK03016.
OMAiYVCHGKI.
OrthoDBiEOG78D7XD.
PhylomeDBiQ92399.

Enzyme and pathway databases

ReactomeiR-SPO-113418. Formation of the Early Elongation Complex.
R-SPO-1834949. Cytosolic sensors of pathogen-associated DNA.
R-SPO-674695. RNA Polymerase II Pre-transcription Events.
R-SPO-6781823. Formation of TC-NER Pre-Incision Complex.
R-SPO-6782135. Dual incision in TC-NER.
R-SPO-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-SPO-72086. mRNA Capping.
R-SPO-72165. mRNA Splicing - Minor Pathway.
R-SPO-73762. RNA Polymerase I Transcription Initiation.
R-SPO-73776. RNA Polymerase II Promoter Escape.
R-SPO-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SPO-75953. RNA Polymerase II Transcription Initiation.
R-SPO-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SPO-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

EvolutionaryTraceiQ92399.
NextBioi20800943.
PROiQ92399.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
InterProiIPR012340. NA-bd_OB-fold.
IPR005570. RNA_pol_Rpb8.
[Graphical view]
PANTHERiPTHR10917. PTHR10917. 1 hit.
PfamiPF03870. RNA_pol_Rpb8. 1 hit.
[Graphical view]
SMARTiSM00658. RPOL8c. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gene organization and protein sequence of the small subunits of Schizosaccharomyces pombe RNA polymerase II."
    Sakurai H., Ishihama A.
    Gene 196:165-174(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 972 / ATCC 24843.
  2. "Structural and functional studies of the subunits shared by nuclear RNA polymerases I, II and III of the fission yeast Schizosaccharomyces pombe."
    Shpakovski G.V., Lebedenko E.N.
    Tsitologiia 39:122-123(1997)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 972 / ATCC 24843.
  3. "Structural and functional characterization of rpb8+ gene of Schizosaccharomyces pombe encoding the subunit of RNA polymerase I-III specific to eukaryotes."
    Shpakovski G.V., Proshkin S.A., Kayushin A.L., Korosteleva M.L., Lebedenko E.N.
    Bioorg. Khim. 24:118-124(1998)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. "Sequence divergence of the RNA polymerase shared subunit ABC14.5 (Rpb8) selectively affects RNA polymerase III assembly in Saccharomyces cerevisiae."
    Voutsina A., Riva M., Carles C., Alexandraki D.
    Nucleic Acids Res. 27:1047-1055(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiRPAB3_SCHPO
AccessioniPrimary (citable) accession number: Q92399
Secondary accession number(s): O13459, O13460
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 15, 1998
Last modified: December 9, 2015
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.