Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q92398 (SPM1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase spm1

Short name=MAP kinase spm1
EC=2.7.11.24
Alternative name(s):
MAP kinase pmk1
Gene names
Name:spm1
Synonyms:pmk1
ORF Names:SPBC119.08
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length422 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulates cell integrity and functions coordinately with the protein kinase C pathway (pck1 and pck2). Involved the regulation of wall architecture, cell shape, cytokinesis in exponential and stationary phase, and metabolism of ions.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Enzyme regulation

Activated by tyrosine and threonine phosphorylation by skh1/pek1.

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-186 and Tyr-188, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Biological processCell cycle
Cell shape
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processMAPK cascade

Inferred from direct assay PubMed 10365961. Source: PomBase

activation of bipolar cell growth

Inferred from mutant phenotype PubMed 20501954. Source: PomBase

cellular sodium ion homeostasis

Inferred from sequence or structural similarity PubMed 9762918. Source: PomBase

fungal-type cell wall organization or biogenesis

Inferred from mutant phenotype Ref.1. Source: PomBase

mitotic cytokinesis

Inferred from mutant phenotype Ref.1. Source: PomBase

positive regulation of calcium ion transport into cytosol

Inferred from mutant phenotype PubMed 21811607. Source: PomBase

positive regulation of calcium-mediated signaling

Inferred from mutant phenotype PubMed 16928959. Source: PomBase

protein phosphorylation

Inferred from sequence model. Source: PomBase

regulation of cell shape

Inferred from mutant phenotype Ref.2. Source: PomBase

   Cellular_componentcell division site

Inferred from direct assay PubMed 16823372. Source: PomBase

cytoplasm

Inferred from direct assay PubMed 16291757. Source: PomBase

cytosol

Inferred from direct assay PubMed 16823372. Source: PomBase

mitotic spindle pole body

Inferred from direct assay PubMed 16823372. Source: PomBase

nucleus

Inferred from direct assay PubMed 16291757PubMed 16823372. Source: PomBase

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

MAP kinase activity

Inferred from sequence orthology Ref.1. Source: PomBase

protein binding

Inferred from physical interaction. Source: PomBase

protein serine/threonine kinase activity

Inferred from direct assay PubMed 12931193. Source: PomBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 422422Mitogen-activated protein kinase spm1
PRO_0000186341

Regions

Domain21 – 314294Protein kinase
Nucleotide binding27 – 359ATP By similarity
Motif186 – 1883TXY
Compositional bias389 – 3946Poly-Ser

Sites

Active site1491Proton acceptor By similarity
Binding site521ATP By similarity

Amino acid modifications

Modified residue1861Phosphothreonine Ref.4
Modified residue1881Phosphotyrosine Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q92398 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: FD02521E64E8BF82

FASTA42248,262
        10         20         30         40         50         60 
MDRRHRVYRV FNQEMYVEPN FKVVKELGQG AYGIVCAARN VASKDQEAVA IKKITNVFSK 

        70         80         90        100        110        120 
SILTKRALRE IKLLIHFRNH RNITCIYDLD IINPYNFNEV YIYEELMEAD LNAIIKSGQP 

       130        140        150        160        170        180 
LTDAHFQSFI YQILCGLKYI HSANVIHRDL KPGNLLVNAD CELKICDFGL ARGCSENPEE 

       190        200        210        220        230        240 
NPGFMTEYVA TRWYRAPEIM LSFSSYHKGI DVWSVGCILA ELLGGTPLFK GKDFVHQLNL 

       250        260        270        280        290        300 
ILHQLGTPDE ETLSHISSSR AQEYVRSLPK QRPIPFETNF PKANPLALDL LAKLLAFDPN 

       310        320        330        340        350        360 
RRISVDDALE HPYLAVWHDP SDEPVCDSVF DFSFEYIEDA NELRRVILDE VLNFRQKVRR 

       370        380        390        400        410        420 
RSHPTNPTVN IPQPAQTVPS NDNGSFNVSS SSSSQTSNKK RHDHSYNETA AIDHKSDDNR 


HN 

« Hide

References

« Hide 'large scale' references
[1]"The fission yeast pmk1+ gene encodes a novel mitogen-activated protein kinase homolog which regulates cell integrity and functions coordinately with the protein kinase C pathway."
Toda T., Dhut S., Superti-Furga G., Gotoh G., Nishida E., Sugiura R., Kuno T.
Mol. Cell. Biol. 16:6752-6764(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Spm1, a stress-activated MAP kinase that regulates morphogenesis in S.pombe."
Zaitsevskaya-Carter T., Cooper J.A.
EMBO J. 16:1318-1331(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[4]"Phosphoproteome analysis of fission yeast."
Wilson-Grady J.T., Villen J., Gygi S.P.
J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-186 AND TYR-188, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X98243 Genomic DNA. Translation: CAA66899.1.
U65405 Genomic DNA. Translation: AAC49707.1.
CU329671 Genomic DNA. Translation: CAA17923.1.
PIRT39306.
RefSeqNP_595289.1. NM_001021196.2.

3D structure databases

ProteinModelPortalQ92398.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid276464. 128 interactions.
MINTMINT-4701538.
STRING4896.SPBC119.08-1.

Proteomic databases

MaxQBQ92398.
PaxDbQ92398.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBC119.08.1; SPBC119.08.1:pep; SPBC119.08.
GeneID2539920.
KEGGspo:SPBC119.08.

Organism-specific databases

PomBaseSPBC119.08.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
KOK04464.
OMAFMPKKPF.
OrthoDBEOG7K3TWD.
PhylomeDBQ92398.

Enzyme and pathway databases

BRENDA2.7.11.24. 5615.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20801063.

Entry information

Entry nameSPM1_SCHPO
AccessionPrimary (citable) accession number: Q92398
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 11, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names