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Protein

Mitogen-activated protein kinase spm1

Gene

spm1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates cell integrity and functions coordinately with the protein kinase C pathway (pck1 and pck2). Involved the regulation of wall architecture, cell shape, cytokinesis in exponential and stationary phase, and metabolism of ions.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation by skh1/pek1.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei52ATPPROSITE-ProRule annotation1
Active sitei149Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 35ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: PomBase
  • protein kinase activity Source: PomBase

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • MAPK cascade Source: PomBase
  • positive regulation of calcium ion import across plasma membrane Source: PomBase
  • positive regulation of calcium-mediated signaling Source: PomBase
  • positive regulation of cell wall organization or biogenesis Source: PomBase
  • positive regulation of DNA binding transcription factor activity Source: PomBase
  • regulation of cell shape Source: UniProtKB-KW

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell shape
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24 5613
ReactomeiR-SPO-110056 MAPK3 (ERK1) activation
R-SPO-112409 RAF-independent MAPK1/3 activation
R-SPO-112411 MAPK1 (ERK2) activation
R-SPO-198753 ERK/MAPK targets
R-SPO-202670 ERKs are inactivated
R-SPO-3371453 Regulation of HSF1-mediated heat shock response
R-SPO-445144 Signal transduction by L1
R-SPO-450341 Activation of the AP-1 family of transcription factors
R-SPO-5673001 RAF/MAP kinase cascade
R-SPO-5674135 MAP2K and MAPK activation
R-SPO-5674499 Negative feedback regulation of MAPK pathway
R-SPO-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase spm1 (EC:2.7.11.24)
Short name:
MAP kinase spm1
Alternative name(s):
MAP kinase pmk1
Gene namesi
Name:spm1
Synonyms:pmk1
ORF Names:SPBC119.08
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC119.08
PomBaseiSPBC119.08

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863411 – 422Mitogen-activated protein kinase spm1Add BLAST422

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei186Phosphothreonine1 Publication1
Modified residuei188Phosphotyrosine1 Publication1

Post-translational modificationi

Dually phosphorylated on Thr-186 and Tyr-188, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ92398
PaxDbiQ92398
PRIDEiQ92398

PTM databases

iPTMnetiQ92398

Interactioni

Protein-protein interaction databases

BioGridi276464, 131 interactors
STRINGi4896.SPBC119.08.1

Structurei

3D structure databases

ProteinModelPortaliQ92398
SMRiQ92398
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 314Protein kinasePROSITE-ProRule annotationAdd BLAST294

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi186 – 188TXY3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi389 – 394Poly-Ser6

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000233024
InParanoidiQ92398
KOiK04464
OMAiASDEPDC
OrthoDBiEOG092C2FL8
PhylomeDBiQ92398

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q92398-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRRHRVYRV FNQEMYVEPN FKVVKELGQG AYGIVCAARN VASKDQEAVA
60 70 80 90 100
IKKITNVFSK SILTKRALRE IKLLIHFRNH RNITCIYDLD IINPYNFNEV
110 120 130 140 150
YIYEELMEAD LNAIIKSGQP LTDAHFQSFI YQILCGLKYI HSANVIHRDL
160 170 180 190 200
KPGNLLVNAD CELKICDFGL ARGCSENPEE NPGFMTEYVA TRWYRAPEIM
210 220 230 240 250
LSFSSYHKGI DVWSVGCILA ELLGGTPLFK GKDFVHQLNL ILHQLGTPDE
260 270 280 290 300
ETLSHISSSR AQEYVRSLPK QRPIPFETNF PKANPLALDL LAKLLAFDPN
310 320 330 340 350
RRISVDDALE HPYLAVWHDP SDEPVCDSVF DFSFEYIEDA NELRRVILDE
360 370 380 390 400
VLNFRQKVRR RSHPTNPTVN IPQPAQTVPS NDNGSFNVSS SSSSQTSNKK
410 420
RHDHSYNETA AIDHKSDDNR HN
Length:422
Mass (Da):48,262
Last modified:February 1, 1997 - v1
Checksum:iFD02521E64E8BF82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98243 Genomic DNA Translation: CAA66899.1
U65405 Genomic DNA Translation: AAC49707.1
CU329671 Genomic DNA Translation: CAA17923.1
PIRiT39306
RefSeqiNP_595289.1, NM_001021196.2

Genome annotation databases

EnsemblFungiiSPBC119.08.1; SPBC119.08.1:pep; SPBC119.08
GeneIDi2539920
KEGGispo:SPBC119.08

Similar proteinsi

Entry informationi

Entry nameiSPM1_SCHPO
AccessioniPrimary (citable) accession number: Q92398
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 23, 2018
This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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