Q92396 (TYRO_PODAS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosinase
EC=1.14.18.1

Alternative name(s):
Monophenol monooxygenase

Gene names

Name:

TYR

Organism

Podospora anserina (Pleurage anserina)

Taxonomic identifier

5145 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Sordariales › Lasiosphaeriaceae › Podospora

Protein attributes

Sequence length	574 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds.
Catalytic activity	2 L-dopa + O2 = 2 dopaquinone + 2 H2O. L-tyrosine + O2 = dopaquinone + H2O.
Cofactor	Binds 2 copper ions per subunit By similarity.
Sequence similarities	Belongs to the tyrosinase family.

Ontologies

Keywords
Biological process	Melanin biosynthesis
Ligand	Copper Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Thioether bond
Gene Ontology (GO)
Biological_process	melanin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW monophenol monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 574	574	Tyrosinase		PRO_0000186734
Sites
	Metal binding	67	1	Copper A By similarity
	Metal binding	95	1	Copper A By similarity
	Metal binding	104	1	Copper A By similarity
	Metal binding	275	1	Copper B By similarity
	Metal binding	279	1	Copper B By similarity
	Metal binding	304	1	Copper B By similarity
Amino acid modifications
	Cross-link	93 ↔ 95		2'-(S-cysteinyl)-histidine (Cys-His) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92396 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 7020A9AAF5CDBE8F	FASTA	574	64,181
	10 20 30 40 50 60 MSTTGNIAIT GIPTTAGPDG SFPLRRELRD LQRNYPDHFN LLVLALKDFQ ALNESVQTSY 70 80 90 100 110 120 YQIAGIHGLP YKPWNNVGSN SDWQSTSGFG GYCTHSSILF LTWHRPYLAL FEQALYNSIQ 130 140 150 160 170 180 KIANQFPQGP LRTKYVEAAK TFRMPYFDWA SQPPSGSSAF PSAFTAPSLQ VVDVDGKTKS 190 200 210 220 230 240 TANPIYRFVF HPVNPSPGDF PRQWSRFPTT VRYPNPRTGQ SQDNRVAPIL ANELASLRTN 250 260 270 280 290 300 VSLLLLSYTN FDAFSFNRWD PNMTPGEFGS LEDVHNEIHD RTGGGGHMSS LDVSSFDPLF 310 320 330 340 350 360 WFHHTNVDRL WAIWQDLNPD NFLTPRPAPY STFNSTEGES QTKDTPLTPF WDKSATKFWT 370 380 390 400 410 420 SEEIKDTTTT FGYAYPETQE WKYRTGSEYQ TSIRQAVTTL YGTNVFANFA AANVQARATE 430 440 450 460 470 480 HTELIKSLSL AAPPPSAPIT AEKPLLITQE MKASPIPEHL QHLAPNNKYP EWVVNIRAQK 490 500 510 520 530 540 HGLHGAFRVI VFLGPIDESD PDSWQTEFNT VGRVSVLGRS TQGPTTTKCA KCITDAADEL 550 560 570 MISGTVPLTS ALLQDIVNEN TASIACSQRK WCRI

References

[1]	"Molecular and functional characterization of the tyrosinase gene of the filamentous fungus Podospora anserina." Marbach K., Stahl U. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: S1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U66808 mRNA. Translation: AAB07516.1. U66807 Genomic DNA. Translation: AAB07484.1.
3D structure databases
ProteinModelPortal	Q92396.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.1280.10. 1 hit.
InterPro	IPR016216. Monophenol_mOase_fun. IPR002227. Tyrosinase. IPR008922. Unchr_di-copper_centre. [Graphical view]
Pfam	PF00264. Tyrosinase. 1 hit. [Graphical view]
PIRSF	PIRSF000340. MPO_fungal. 1 hit.
PRINTS	PR00092. TYROSINASE.
SUPFAM	SSF48056. Di-copper_centre. 1 hit.
PROSITE	PS00497. TYROSINASE_1. 1 hit. PS00498. TYROSINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYRO_PODAS

Accession

Primary (citable) accession number: Q92396

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	March 6, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program