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Q92383

- MAG1_SCHPO

UniProt

Q92383 - MAG1_SCHPO

Protein

DNA-3-methyladenine glycosylase 1

Gene

mag1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine or 7-methyladenine from the damaged DNA polymer formed by alkylation lesions. Can release ethylated and propylated bases from DNA in addition to 3-methyladenine.

    Catalytic activityi

    Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei150 – 1501Determinant for substrate specificity and/or activityBy similarity
    Active sitei170 – 1701Proton acceptorBy similarity

    GO - Molecular functioni

    1. alkylated DNA binding Source: PomBase
    2. alkylbase DNA N-glycosylase activity Source: PomBase
    3. damaged DNA binding Source: PomBase
    4. DNA-1,N6-ethenoadenine N-glycosylase activity Source: PomBase
    5. DNA-3-methyladenine glycosylase activity Source: PomBase
    6. DNA-3-methylguanine glycosylase activity Source: UniProtKB-EC
    7. DNA-7-methyladenine glycosylase activity Source: UniProtKB-EC
    8. DNA-7-methylguanine glycosylase activity Source: PomBase

    GO - Biological processi

    1. base-excision repair, AP site formation Source: PomBase
    2. depurination Source: PomBase
    3. DNA dealkylation involved in DNA repair Source: PomBase
    4. recombinational repair Source: PomBase

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    DNA damage, DNA repair

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-3-methyladenine glycosylase 1 (EC:3.2.2.21)
    Alternative name(s):
    3-methyladenine DNA glycosidase 1
    3MEA DNA glycosylase 1
    Gene namesi
    Name:mag1
    ORF Names:SPAPB24D3.04c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAPB24D3.04c.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: PomBase
    2. protein-DNA complex Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 228228DNA-3-methyladenine glycosylase 1PRO_0000194881Add
    BLAST

    Proteomic databases

    MaxQBiQ92383.

    Interactioni

    Protein-protein interaction databases

    BioGridi279981. 30 interactions.
    MINTiMINT-4701520.
    STRINGi4896.SPAPB24D3.04c-1.

    Structurei

    Secondary structure

    1
    228
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi17 – 237
    Helixi27 – 3610
    Turni44 – 474
    Helixi50 – 6011
    Beta strandi61 – 633
    Helixi65 – 7612
    Helixi80 – 823
    Helixi87 – 926
    Helixi95 – 1017
    Helixi105 – 12016
    Beta strandi121 – 1233
    Helixi126 – 1294
    Helixi134 – 1418
    Helixi149 – 15810
    Helixi171 – 18010
    Helixi189 – 1968
    Helixi197 – 1993
    Helixi203 – 21210
    Helixi213 – 2153

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3S6IX-ray2.28A/D1-228[»]
    ProteinModelPortaliQ92383.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0122.
    HOGENOMiHOG000261908.
    KOiK01247.
    OMAiVEMFLIY.
    OrthoDBiEOG78SQVJ.
    PhylomeDBiQ92383.

    Family and domain databases

    Gene3Di1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    InterProiIPR000035. Alkylbase_DNA_glycsylse_CS.
    IPR011257. DNA_glycosylase.
    IPR003265. HhH-GPD_domain.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR023170. HTH_base_excis_C.
    [Graphical view]
    PfamiPF00730. HhH-GPD. 1 hit.
    [Graphical view]
    SMARTiSM00478. ENDO3c. 1 hit.
    SM00278. HhH1. 1 hit.
    [Graphical view]
    SUPFAMiSSF48150. SSF48150. 1 hit.
    PROSITEiPS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q92383-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTLDIEEKEE IVTSLTKAEI HLSGLDENWK RLVKLVGNYR PNRSMEKKEP    50
    YEELIRAVAS QQLHSKAANA IFNRFKSISN NGQFPTPEEI RDMDFEIMRA 100
    CGFSARKIDS LKSIAEATIS GLIPTKEEAE RLSNEELIER LTQIKGIGRW 150
    TVEMLLIFSL NRDDVMPADD LSIRNGYRYL HRLPKIPTKM YVLKHSEICA 200
    PFRTAAAWYL WKTSKLADYT KPVRPKKH 228
    Length:228
    Mass (Da):26,381
    Last modified:February 1, 1997 - v1
    Checksum:i4D134AB1EC377095
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U76637 mRNA. Translation: AAC49524.1.
    CU329670 Genomic DNA. Translation: CAC36900.1.
    PIRiJC5177.
    RefSeqiNP_593991.1. NM_001019417.2.

    Genome annotation databases

    EnsemblFungiiSPAPB24D3.04c.1; SPAPB24D3.04c.1:pep; SPAPB24D3.04c.
    GeneIDi2543565.
    KEGGispo:SPAPB24D3.04c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U76637 mRNA. Translation: AAC49524.1 .
    CU329670 Genomic DNA. Translation: CAC36900.1 .
    PIRi JC5177.
    RefSeqi NP_593991.1. NM_001019417.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3S6I X-ray 2.28 A/D 1-228 [» ]
    ProteinModelPortali Q92383.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 279981. 30 interactions.
    MINTi MINT-4701520.
    STRINGi 4896.SPAPB24D3.04c-1.

    Proteomic databases

    MaxQBi Q92383.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAPB24D3.04c.1 ; SPAPB24D3.04c.1:pep ; SPAPB24D3.04c .
    GeneIDi 2543565.
    KEGGi spo:SPAPB24D3.04c.

    Organism-specific databases

    PomBasei SPAPB24D3.04c.

    Phylogenomic databases

    eggNOGi COG0122.
    HOGENOMi HOG000261908.
    KOi K01247.
    OMAi VEMFLIY.
    OrthoDBi EOG78SQVJ.
    PhylomeDBi Q92383.

    Miscellaneous databases

    NextBioi 20804573.

    Family and domain databases

    Gene3Di 1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    InterProi IPR000035. Alkylbase_DNA_glycsylse_CS.
    IPR011257. DNA_glycosylase.
    IPR003265. HhH-GPD_domain.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR023170. HTH_base_excis_C.
    [Graphical view ]
    Pfami PF00730. HhH-GPD. 1 hit.
    [Graphical view ]
    SMARTi SM00478. ENDO3c. 1 hit.
    SM00278. HhH1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48150. SSF48150. 1 hit.
    PROSITEi PS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a cDNA encoding a 3-methyladenine DNA glycosylase from the fission yeast Schizosaccharomyces pombe."
      Memisoglu A., Samson L.
      Gene 177:229-235(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.

    Entry informationi

    Entry nameiMAG1_SCHPO
    AccessioniPrimary (citable) accession number: Q92383
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3