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Q92383

- MAG1_SCHPO

UniProt

Q92383 - MAG1_SCHPO

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Protein

DNA-3-methyladenine glycosylase 1

Gene

mag1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine or 7-methyladenine from the damaged DNA polymer formed by alkylation lesions. Can release ethylated and propylated bases from DNA in addition to 3-methyladenine.

Catalytic activityi

Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei150 – 1501Determinant for substrate specificity and/or activityBy similarity
Active sitei170 – 1701Proton acceptorBy similarity

GO - Molecular functioni

  1. alkylated DNA binding Source: PomBase
  2. alkylbase DNA N-glycosylase activity Source: PomBase
  3. damaged DNA binding Source: PomBase
  4. DNA-1,N6-ethenoadenine N-glycosylase activity Source: PomBase
  5. DNA-3-methyladenine glycosylase activity Source: PomBase
  6. DNA-3-methylguanine glycosylase activity Source: UniProtKB-EC
  7. DNA-7-methyladenine glycosylase activity Source: UniProtKB-EC
  8. DNA-7-methylguanine glycosylase activity Source: PomBase

GO - Biological processi

  1. base-excision repair, AP site formation Source: PomBase
  2. depurination Source: PomBase
  3. DNA dealkylation involved in DNA repair Source: PomBase
  4. recombinational repair Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-3-methyladenine glycosylase 1 (EC:3.2.2.21)
Alternative name(s):
3-methyladenine DNA glycosidase 1
3MEA DNA glycosylase 1
Gene namesi
Name:mag1
ORF Names:SPAPB24D3.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAPB24D3.04c.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: PomBase
  2. protein-DNA complex Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 228228DNA-3-methyladenine glycosylase 1PRO_0000194881Add
BLAST

Proteomic databases

MaxQBiQ92383.

Interactioni

Protein-protein interaction databases

BioGridi279981. 30 interactions.
MINTiMINT-4701520.
STRINGi4896.SPAPB24D3.04c-1.

Structurei

Secondary structure

1
228
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi17 – 237
Helixi27 – 3610
Turni44 – 474
Helixi50 – 6011
Beta strandi61 – 633
Helixi65 – 7612
Helixi80 – 823
Helixi87 – 926
Helixi95 – 1017
Helixi105 – 12016
Beta strandi121 – 1233
Helixi126 – 1294
Helixi134 – 1418
Helixi149 – 15810
Helixi171 – 18010
Helixi189 – 1968
Helixi197 – 1993
Helixi203 – 21210
Helixi213 – 2153

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3S6IX-ray2.28A/D1-228[»]
ProteinModelPortaliQ92383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0122.
HOGENOMiHOG000261908.
InParanoidiQ92383.
KOiK01247.
OMAiVEMFLIY.
OrthoDBiEOG78SQVJ.
PhylomeDBiQ92383.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
InterProiIPR000035. Alkylbase_DNA_glycsylse_CS.
IPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
[Graphical view]
PfamiPF00730. HhH-GPD. 1 hit.
[Graphical view]
SMARTiSM00478. ENDO3c. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
PROSITEiPS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92383-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTLDIEEKEE IVTSLTKAEI HLSGLDENWK RLVKLVGNYR PNRSMEKKEP
60 70 80 90 100
YEELIRAVAS QQLHSKAANA IFNRFKSISN NGQFPTPEEI RDMDFEIMRA
110 120 130 140 150
CGFSARKIDS LKSIAEATIS GLIPTKEEAE RLSNEELIER LTQIKGIGRW
160 170 180 190 200
TVEMLLIFSL NRDDVMPADD LSIRNGYRYL HRLPKIPTKM YVLKHSEICA
210 220
PFRTAAAWYL WKTSKLADYT KPVRPKKH
Length:228
Mass (Da):26,381
Last modified:February 1, 1997 - v1
Checksum:i4D134AB1EC377095
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U76637 mRNA. Translation: AAC49524.1.
CU329670 Genomic DNA. Translation: CAC36900.1.
PIRiJC5177.
RefSeqiNP_593991.1. NM_001019417.2.

Genome annotation databases

EnsemblFungiiSPAPB24D3.04c.1; SPAPB24D3.04c.1:pep; SPAPB24D3.04c.
GeneIDi2543565.
KEGGispo:SPAPB24D3.04c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U76637 mRNA. Translation: AAC49524.1 .
CU329670 Genomic DNA. Translation: CAC36900.1 .
PIRi JC5177.
RefSeqi NP_593991.1. NM_001019417.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3S6I X-ray 2.28 A/D 1-228 [» ]
ProteinModelPortali Q92383.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 279981. 30 interactions.
MINTi MINT-4701520.
STRINGi 4896.SPAPB24D3.04c-1.

Proteomic databases

MaxQBi Q92383.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAPB24D3.04c.1 ; SPAPB24D3.04c.1:pep ; SPAPB24D3.04c .
GeneIDi 2543565.
KEGGi spo:SPAPB24D3.04c.

Organism-specific databases

PomBasei SPAPB24D3.04c.

Phylogenomic databases

eggNOGi COG0122.
HOGENOMi HOG000261908.
InParanoidi Q92383.
KOi K01247.
OMAi VEMFLIY.
OrthoDBi EOG78SQVJ.
PhylomeDBi Q92383.

Miscellaneous databases

NextBioi 20804573.

Family and domain databases

Gene3Di 1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
InterProi IPR000035. Alkylbase_DNA_glycsylse_CS.
IPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
[Graphical view ]
Pfami PF00730. HhH-GPD. 1 hit.
[Graphical view ]
SMARTi SM00478. ENDO3c. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view ]
SUPFAMi SSF48150. SSF48150. 1 hit.
PROSITEi PS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a cDNA encoding a 3-methyladenine DNA glycosylase from the fission yeast Schizosaccharomyces pombe."
    Memisoglu A., Samson L.
    Gene 177:229-235(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiMAG1_SCHPO
AccessioniPrimary (citable) accession number: Q92383
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: October 29, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3