Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA-3-methyladenine glycosylase 1

Gene

mag1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine or 7-methyladenine from the damaged DNA polymer formed by alkylation lesions. Can release ethylated and propylated bases from DNA in addition to 3-methyladenine.

Catalytic activityi

Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei150Determinant for substrate specificity and/or activityBy similarity1
Active sitei170Proton acceptorBy similarity1

GO - Molecular functioni

  • alkylated DNA binding Source: PomBase
  • alkylbase DNA N-glycosylase activity Source: PomBase
  • damaged DNA binding Source: PomBase
  • DNA-1,N6-ethenoadenine N-glycosylase activity Source: PomBase
  • DNA-3-methyladenine glycosylase activity Source: PomBase
  • DNA-3-methylguanine glycosylase activity Source: UniProtKB-EC
  • DNA-7-methyladenine glycosylase activity Source: UniProtKB-EC
  • DNA-7-methylguanine glycosylase activity Source: PomBase

GO - Biological processi

  • base-excision repair, AP site formation Source: PomBase
  • depurination Source: PomBase
  • DNA dealkylation involved in DNA repair Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-3-methyladenine glycosylase 1 (EC:3.2.2.21)
Alternative name(s):
3-methyladenine DNA glycosidase 1
3MEA DNA glycosylase 1
Gene namesi
Name:mag1
ORF Names:SPAPB24D3.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAPB24D3.04c.
PomBaseiSPAPB24D3.04c. mag1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: PomBase
  • protein-DNA complex Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001948811 – 228DNA-3-methyladenine glycosylase 1Add BLAST228

Proteomic databases

MaxQBiQ92383.
PRIDEiQ92383.

Interactioni

Protein-protein interaction databases

BioGridi279981. 30 interactors.
MINTiMINT-4701520.

Structurei

Secondary structure

1228
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi17 – 23Combined sources7
Helixi27 – 36Combined sources10
Turni44 – 47Combined sources4
Helixi50 – 60Combined sources11
Beta strandi61 – 63Combined sources3
Helixi65 – 76Combined sources12
Helixi80 – 82Combined sources3
Helixi87 – 92Combined sources6
Helixi95 – 101Combined sources7
Helixi105 – 120Combined sources16
Beta strandi121 – 123Combined sources3
Helixi126 – 129Combined sources4
Helixi134 – 141Combined sources8
Helixi149 – 158Combined sources10
Helixi171 – 180Combined sources10
Helixi189 – 196Combined sources8
Helixi197 – 199Combined sources3
Helixi203 – 212Combined sources10
Helixi213 – 215Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3S6IX-ray2.28A/D1-228[»]
ProteinModelPortaliQ92383.
SMRiQ92383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000261908.
InParanoidiQ92383.
OMAiNYLIFAE.
OrthoDBiEOG092C51YL.
PhylomeDBiQ92383.

Family and domain databases

CDDicd00056. ENDO3c. 1 hit.
Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
InterProiIPR000035. Alkylbase_DNA_glycsylse_CS.
IPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR023170. HTH_base_excis_C.
[Graphical view]
PfamiPF00730. HhH-GPD. 1 hit.
[Graphical view]
SMARTiSM00478. ENDO3c. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
PROSITEiPS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92383-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLDIEEKEE IVTSLTKAEI HLSGLDENWK RLVKLVGNYR PNRSMEKKEP
60 70 80 90 100
YEELIRAVAS QQLHSKAANA IFNRFKSISN NGQFPTPEEI RDMDFEIMRA
110 120 130 140 150
CGFSARKIDS LKSIAEATIS GLIPTKEEAE RLSNEELIER LTQIKGIGRW
160 170 180 190 200
TVEMLLIFSL NRDDVMPADD LSIRNGYRYL HRLPKIPTKM YVLKHSEICA
210 220
PFRTAAAWYL WKTSKLADYT KPVRPKKH
Length:228
Mass (Da):26,381
Last modified:February 1, 1997 - v1
Checksum:i4D134AB1EC377095
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76637 mRNA. Translation: AAC49524.1.
CU329670 Genomic DNA. Translation: CAC36900.1.
PIRiJC5177.
RefSeqiNP_593991.1. NM_001019417.2.

Genome annotation databases

EnsemblFungiiSPAPB24D3.04c.1; SPAPB24D3.04c.1:pep; SPAPB24D3.04c.
GeneIDi2543565.
KEGGispo:SPAPB24D3.04c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76637 mRNA. Translation: AAC49524.1.
CU329670 Genomic DNA. Translation: CAC36900.1.
PIRiJC5177.
RefSeqiNP_593991.1. NM_001019417.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3S6IX-ray2.28A/D1-228[»]
ProteinModelPortaliQ92383.
SMRiQ92383.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279981. 30 interactors.
MINTiMINT-4701520.

Proteomic databases

MaxQBiQ92383.
PRIDEiQ92383.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAPB24D3.04c.1; SPAPB24D3.04c.1:pep; SPAPB24D3.04c.
GeneIDi2543565.
KEGGispo:SPAPB24D3.04c.

Organism-specific databases

EuPathDBiFungiDB:SPAPB24D3.04c.
PomBaseiSPAPB24D3.04c. mag1.

Phylogenomic databases

HOGENOMiHOG000261908.
InParanoidiQ92383.
OMAiNYLIFAE.
OrthoDBiEOG092C51YL.
PhylomeDBiQ92383.

Miscellaneous databases

PROiQ92383.

Family and domain databases

CDDicd00056. ENDO3c. 1 hit.
Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
InterProiIPR000035. Alkylbase_DNA_glycsylse_CS.
IPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR023170. HTH_base_excis_C.
[Graphical view]
PfamiPF00730. HhH-GPD. 1 hit.
[Graphical view]
SMARTiSM00478. ENDO3c. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
PROSITEiPS00516. ALKYLBASE_DNA_GLYCOS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAG1_SCHPO
AccessioniPrimary (citable) accession number: Q92383
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.