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Protein

DNA-3-methyladenine glycosylase 1

Gene

mag1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine or 7-methyladenine from the damaged DNA polymer formed by alkylation lesions. Can release ethylated and propylated bases from DNA in addition to 3-methyladenine.

Catalytic activityi

Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei150Determinant for substrate specificity and/or activityBy similarity1
Active sitei170Proton acceptorBy similarity1

GO - Molecular functioni

  • alkylated DNA binding Source: PomBase
  • alkylbase DNA N-glycosylase activity Source: PomBase
  • damaged DNA binding Source: PomBase
  • DNA-1,N6-ethenoadenine N-glycosylase activity Source: PomBase
  • DNA-3-methyladenine glycosylase activity Source: PomBase
  • DNA-3-methylguanine glycosylase activity Source: UniProtKB-EC
  • DNA-7-methyladenine glycosylase activity Source: UniProtKB-EC
  • DNA-7-methylguanine glycosylase activity Source: PomBase

GO - Biological processi

  • base-excision repair, AP site formation Source: PomBase
  • depurination Source: PomBase
  • DNA dealkylation involved in DNA repair Source: PomBase

Keywordsi

Molecular functionHydrolase
Biological processDNA damage, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-3-methyladenine glycosylase 1 (EC:3.2.2.21)
Alternative name(s):
3-methyladenine DNA glycosidase 1
3MEA DNA glycosylase 1
Gene namesi
Name:mag1
ORF Names:SPAPB24D3.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAPB24D3.04c
PomBaseiSPAPB24D3.04c mag1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001948811 – 228DNA-3-methyladenine glycosylase 1Add BLAST228

Proteomic databases

MaxQBiQ92383
PaxDbiQ92383
PRIDEiQ92383

Interactioni

Protein-protein interaction databases

BioGridi279981, 30 interactors
STRINGi4896.SPAPB24D3.04c.1

Structurei

Secondary structure

1228
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi17 – 23Combined sources7
Helixi27 – 36Combined sources10
Turni44 – 47Combined sources4
Helixi50 – 60Combined sources11
Beta strandi61 – 63Combined sources3
Helixi65 – 76Combined sources12
Helixi80 – 82Combined sources3
Helixi87 – 92Combined sources6
Helixi95 – 101Combined sources7
Helixi105 – 120Combined sources16
Beta strandi121 – 123Combined sources3
Helixi126 – 129Combined sources4
Helixi134 – 141Combined sources8
Helixi149 – 158Combined sources10
Helixi171 – 180Combined sources10
Helixi189 – 196Combined sources8
Helixi197 – 199Combined sources3
Helixi203 – 212Combined sources10
Helixi213 – 215Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3S6IX-ray2.28A/D1-228[»]
ProteinModelPortaliQ92383
SMRiQ92383
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000261908
InParanoidiQ92383
OMAiRTVAAWY
OrthoDBiEOG092C51YL
PhylomeDBiQ92383

Family and domain databases

CDDicd00056 ENDO3c, 1 hit
InterProiView protein in InterPro
IPR000035 Alkylbase_DNA_glycsylse_CS
IPR011257 DNA_glycosylase
IPR003265 HhH-GPD_domain
PfamiView protein in Pfam
PF00730 HhH-GPD, 1 hit
SMARTiView protein in SMART
SM00478 ENDO3c, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
PROSITEiView protein in PROSITE
PS00516 ALKYLBASE_DNA_GLYCOS, 1 hit

Sequencei

Sequence statusi: Complete.

Q92383-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLDIEEKEE IVTSLTKAEI HLSGLDENWK RLVKLVGNYR PNRSMEKKEP
60 70 80 90 100
YEELIRAVAS QQLHSKAANA IFNRFKSISN NGQFPTPEEI RDMDFEIMRA
110 120 130 140 150
CGFSARKIDS LKSIAEATIS GLIPTKEEAE RLSNEELIER LTQIKGIGRW
160 170 180 190 200
TVEMLLIFSL NRDDVMPADD LSIRNGYRYL HRLPKIPTKM YVLKHSEICA
210 220
PFRTAAAWYL WKTSKLADYT KPVRPKKH
Length:228
Mass (Da):26,381
Last modified:February 1, 1997 - v1
Checksum:i4D134AB1EC377095
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76637 mRNA Translation: AAC49524.1
CU329670 Genomic DNA Translation: CAC36900.1
PIRiJC5177
RefSeqiNP_593991.1, NM_001019417.2

Genome annotation databases

EnsemblFungiiSPAPB24D3.04c.1; SPAPB24D3.04c.1:pep; SPAPB24D3.04c
GeneIDi2543565
KEGGispo:SPAPB24D3.04c

Similar proteinsi

Entry informationi

Entry nameiMAG1_SCHPO
AccessioniPrimary (citable) accession number: Q92383
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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