Reviewed, UniProtKB/Swiss-Prot Q92375 (TRXB_SCHPO)

Names and origin

Protein names

Recommended name:
    Thioredoxin reductase
    EC=1.8.1.9
Alternative name(s):
    Caffeine resistance protein 4

Gene names

Name:	trr1
Synonyms:	caf4
ORF Names:	SPBC3F6.03

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular response to oxidative stress Inferred from expression pattern. Source: GeneDB_SPombe oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW removal of superoxide radicals Inferred from electronic annotation. Source: InterPro response to caffeine Inferred from mutant phenotype. Source: GeneDB_SPombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro thioredoxin-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	Thioredoxin reductase		PRO_0000166768
Regions
	Nucleotide binding	33 – 45	13	FAD By similarity
	Nucleotide binding	288 – 297	10	FAD By similarity
Amino acid modifications
	Modified residue	192	1	Phosphoserine Ref.5
	Modified residue	278	1	Phosphothreonine Ref.5
	Modified residue	279	1	Phosphoserine Ref.5
	Disulfide bond	142 ↔ 145		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92375-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 3209D979DA303ECA	FASTA	322	34,618
	10 20 30 40 50 60 MTHNKVVIIG SGPAGHTAAI YLARGELKPV MYEGMLANGI AAGGQLTTTT DVENFPGFPD 70 80 90 100 110 120 GINGTTLTEN FRAQSLRFGT EIITETVSKL DLSSRPFKYW LEGAEEEEPH TADSVILATG 130 140 150 160 170 180 ASARRLHITG EDTYWQAGIS ACAVCDGAVP IYRNKPLAVV GGGDSAAEEA QFLTKYGSKV 190 200 210 220 230 240 YVLVRRDKLR ASPIMAKRLL ANPKVEVLWN TVAEEAQGDG KLLNNLRIKN TNTNEVSDLQ 250 260 270 280 290 300 VNGLFYAIGH IPATKLVAEQ IELDEAGYIK TINGTPRTSI PGFFAAGDVQ DKVFRQAITS 310 320 AGSGCQAALL AMHYLEELED TD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A mutation in a thioredoxin reductase homolog suppresses p53-induced growth inhibition in the fission yeast Schizosaccharomyces pombe." Casso D., Beach D. Mol. Gen. Genet. 252:518-529(1996) [PubMed: 8914513] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning of caf1+, caf2+ and caf4+ from Schizosaccharomyces pombe: their involvement in multidrug resistance, UV and pH sensitivity." Benko Z., Sipiczki M., Carr A.M. Mol. Gen. Genet. 260:434-443(1998) [PubMed: 9894913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Molecular characterization and regulation of a gene encoding thioredoxin reductase homolog from the fission Yeast." Hong S.-M., Cho Y.-W., Lim C.-J. Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[5]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-192; THR-278 AND SER-279, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U63713 Genomic DNA. Translation: AAC49569.1. AF535134 Genomic DNA. Translation: AAN01228.1. CU329671 Genomic DNA. Translation: CAA17692.1.
PIR	T40393.
RefSeq	NP_001018848.1.
3D structure databases
HSSP	HSSP built from PDB template 1VDC based on UniProtKB Q39243.
ModBase	Search...
Genome annotation databases
GeneID	3361358.
KEGG	spo:SPBC3F6.03.
Organism-specific databases
GeneDB_Spombe	SPBC3F6.03.
Phylogenomic databases
OMA	Q92375. YRNREVA.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-004726-MON.
BRENDA	1.8.1.9. 653.
Gene expression databases
ArrayExpress	Q92375.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR008255. Pyr_nucl-diS_OxRdtase_2_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR000103. Pyridine_nuc-diS_OxRdtase_2. IPR005982. Thioredox_Rdtase. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00469. PNDRDTASEII.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01292. TRX_reduct. 1 hit.
PROSITE	PS00573. PYRIDINE_REDOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRXB_SCHPO

Accession

Primary (citable) accession number: Q92375

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)