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Q92370

- TRPG_SCHPO

UniProt

Q92370 - TRPG_SCHPO

Protein

Multifunctional tryptophan biosynthesis protein

Gene

trp1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (27 Apr 2001)
      Previous versions | rss
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    Functioni

    Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.

    Catalytic activityi

    N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.
    1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.
    Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei108 – 1081For GATase activityBy similarity
    Active sitei197 – 1971For GATase activityBy similarity
    Active sitei199 – 1991For GATase activityBy similarity

    GO - Molecular functioni

    1. anthranilate synthase activity Source: PomBase
    2. indole-3-glycerol-phosphate lyase activity Source: PomBase
    3. indole-3-glycerol-phosphate synthase activity Source: UniProtKB-EC
    4. phosphoribosylanthranilate isomerase activity Source: PomBase
    5. transferase activity Source: UniProtKB-KW

    GO - Biological processi

    1. glutamine metabolic process Source: UniProtKB-KW
    2. tryptophan biosynthetic process Source: PomBase

    Keywords - Molecular functioni

    Decarboxylase, Isomerase, Lyase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00035; UER00040.
    UPA00035; UER00042.
    UPA00035; UER00043.

    Protein family/group databases

    MEROPSiC26.A25.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Multifunctional tryptophan biosynthesis protein
    Including the following 3 domains:
    Anthranilate synthase component 2 (EC:4.1.3.27)
    Short name:
    AS
    Alternative name(s):
    Anthranilate synthase, glutamine amidotransferase component
    Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
    Short name:
    IGPS
    N-(5'-phosphoribosyl)anthranilate isomerase (EC:5.3.1.24)
    Short name:
    PRAI
    Gene namesi
    Name:trp1
    ORF Names:SPBC1539.09c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC1539.09c.

    Subcellular locationi

    GO - Cellular componenti

    1. anthranilate synthase complex Source: PomBase
    2. cytosol Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 759759Multifunctional tryptophan biosynthesis proteinPRO_0000056865Add
    BLAST

    Proteomic databases

    MaxQBiQ92370.
    PaxDbiQ92370.

    Interactioni

    Protein-protein interaction databases

    BioGridi276517. 3 interactions.
    MINTiMINT-4701389.
    STRINGi4896.SPBC1539.09c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ92370.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini27 – 223197Glutamine amidotransferase type-1Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni257 – 519263Indole-3-glycerol phosphate synthaseAdd
    BLAST
    Regioni536 – 759224N-(5'-phosphoribosyl)anthranilate isomeraseAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0134.
    HOGENOMiHOG000280459.
    KOiK13501.
    OMAiPVLGVCM.
    OrthoDBiEOG78WM1Q.
    PhylomeDBiQ92370.

    Family and domain databases

    Gene3Di3.20.20.70. 2 hits.
    3.40.50.880. 1 hit.
    HAMAPiMF_00134_B. IGPS_B.
    MF_00135. PRAI.
    InterProiIPR013785. Aldolase_TIM.
    IPR016302. Anthranilate_synth_II.
    IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR013798. Indole-3-glycerol_P_synth.
    IPR001468. Indole-3-GlycerolPSynthase_CS.
    IPR001240. PRAI_dom.
    IPR011060. RibuloseP-bd_barrel.
    IPR006221. TrpG/PapA_dom.
    [Graphical view]
    PfamiPF00117. GATase. 1 hit.
    PF00218. IGPS. 1 hit.
    PF00697. PRAI. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001382. TrpG-trpC-trpF. 1 hit.
    SUPFAMiSSF51366. SSF51366. 3 hits.
    SSF52317. SSF52317. 1 hit.
    TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
    PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
    PS00614. IGPS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q92370-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEKVDVGES VKGDASENAV KEVAERPIVM IDNYDSFTWN VVQYLSNLEK    50
    RYPIMVFRND EITVDELEKL NPLKLVLSPG PGHPARDGGI CNEAISRFAG 100
    KIPILGVCMG LQCIFETMGG KVDSAGEIIH GKVSKINHDG LGFYQGIPQN 150
    ISVTRYHSLA GKISSLPDCL DVTSWTENGV IMGARHKKYA IEGVQYHPES 200
    ILSEYGKEYI QNFLNLTAGT WEENGIVMPT KNNAFNAAMR ENSNSVSSTK 250
    IRKQESILEK IHAQRLIDIA ESKRKPGLSV GDLQTYLNLN IAPPCINFYE 300
    RLKQSKPALM AEVKRASPSK GDIKLDANAA IQALTYAQVG ASVISVLTEP 350
    KWFKGSLNDL FVARKAVEHV ANRPAILRKD FIIDPYQIME ARLNGADSVL 400
    LIVAMLSREQ LESLYKFSKS LGMEPLVEVN CAEEMKTAIE LGAKVIGVNN 450
    RNLHSFEVDL STTSKLAEMV PDDVILAALS GISSPADVAH YSSQGVSAVL 500
    VGESLMRASD PAAFARELLN LSSSEISNGK KTSTPLVKVC GTRSLLAAKT 550
    IVESGGDLIG LIFVEKSKRK VDLSVAKEIS HFVHTTNRKH ISPKKAVTGQ 600
    SWFDHQYENL ASSPHPLLVG VFQNQPLEYI RSIIAEVNLD IVQLHGQEPF 650
    EWIHMLDRPV IKVFPLNSSE ISRPNYHIVP LIDAYVGGES GGLGKKVDWE 700
    AASFIPVSYV LAGGLTPKNV QDAISVSRPA VVDVSSGVET DGKQDLEKIK 750
    AFINAVKEL 759
    Length:759
    Mass (Da):83,040
    Last modified:April 27, 2001 - v2
    Checksum:i519E2EA55CB9B212
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti387 – 3871Q → E in CAA70348. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y09137 mRNA. Translation: CAA70348.1.
    CU329671 Genomic DNA. Translation: CAB51341.1.
    PIRiT39468.
    T46566.
    RefSeqiNP_596823.1. NM_001023843.1.

    Genome annotation databases

    EnsemblFungiiSPBC1539.09c.1; SPBC1539.09c.1:pep; SPBC1539.09c.
    GeneIDi2539973.
    KEGGispo:SPBC1539.09c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y09137 mRNA. Translation: CAA70348.1 .
    CU329671 Genomic DNA. Translation: CAB51341.1 .
    PIRi T39468.
    T46566.
    RefSeqi NP_596823.1. NM_001023843.1.

    3D structure databases

    ProteinModelPortali Q92370.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 276517. 3 interactions.
    MINTi MINT-4701389.
    STRINGi 4896.SPBC1539.09c-1.

    Protein family/group databases

    MEROPSi C26.A25.

    Proteomic databases

    MaxQBi Q92370.
    PaxDbi Q92370.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC1539.09c.1 ; SPBC1539.09c.1:pep ; SPBC1539.09c .
    GeneIDi 2539973.
    KEGGi spo:SPBC1539.09c.

    Organism-specific databases

    PomBasei SPBC1539.09c.

    Phylogenomic databases

    eggNOGi COG0134.
    HOGENOMi HOG000280459.
    KOi K13501.
    OMAi PVLGVCM.
    OrthoDBi EOG78WM1Q.
    PhylomeDBi Q92370.

    Enzyme and pathway databases

    UniPathwayi UPA00035 ; UER00040 .
    UPA00035 ; UER00042 .
    UPA00035 ; UER00043 .

    Miscellaneous databases

    NextBioi 20801116.

    Family and domain databases

    Gene3Di 3.20.20.70. 2 hits.
    3.40.50.880. 1 hit.
    HAMAPi MF_00134_B. IGPS_B.
    MF_00135. PRAI.
    InterProi IPR013785. Aldolase_TIM.
    IPR016302. Anthranilate_synth_II.
    IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR013798. Indole-3-glycerol_P_synth.
    IPR001468. Indole-3-GlycerolPSynthase_CS.
    IPR001240. PRAI_dom.
    IPR011060. RibuloseP-bd_barrel.
    IPR006221. TrpG/PapA_dom.
    [Graphical view ]
    Pfami PF00117. GATase. 1 hit.
    PF00218. IGPS. 1 hit.
    PF00697. PRAI. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001382. TrpG-trpC-trpF. 1 hit.
    SUPFAMi SSF51366. SSF51366. 3 hits.
    SSF52317. SSF52317. 1 hit.
    TIGRFAMsi TIGR00566. trpG_papA. 1 hit.
    PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
    PS00614. IGPS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. David C., Couzin N., Lauquin G.J.-M.
      Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: 972 / ATCC 24843.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.

    Entry informationi

    Entry nameiTRPG_SCHPO
    AccessioniPrimary (citable) accession number: Q92370
    Secondary accession number(s): Q9UUF5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: April 27, 2001
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3