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Protein

Multifunctional tryptophan biosynthesis protein

Gene

trp1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.

Catalytic activityi

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.
Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei108 – 1081For GATase activityBy similarity
Active sitei197 – 1971For GATase activityBy similarity
Active sitei199 – 1991For GATase activityBy similarity

GO - Molecular functioni

  1. anthranilate synthase activity Source: PomBase
  2. indole-3-glycerol-phosphate lyase activity Source: PomBase
  3. indole-3-glycerol-phosphate synthase activity Source: UniProtKB-EC
  4. phosphoribosylanthranilate isomerase activity Source: PomBase
  5. transferase activity Source: UniProtKB-KW

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-KW
  2. tryptophan biosynthetic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Isomerase, Lyase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
UPA00035; UER00042.
UPA00035; UER00043.

Protein family/group databases

MEROPSiC26.A25.

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional tryptophan biosynthesis protein
Including the following 3 domains:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Alternative name(s):
Anthranilate synthase, glutamine amidotransferase component
Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
Short name:
IGPS
N-(5'-phosphoribosyl)anthranilate isomerase (EC:5.3.1.24)
Short name:
PRAI
Gene namesi
Name:trp1
ORF Names:SPBC1539.09c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome II

Organism-specific databases

PomBaseiSPBC1539.09c.

Subcellular locationi

GO - Cellular componenti

  1. anthranilate synthase complex Source: PomBase
  2. cytosol Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 759759Multifunctional tryptophan biosynthesis proteinPRO_0000056865Add
BLAST

Proteomic databases

MaxQBiQ92370.
PaxDbiQ92370.

Interactioni

Protein-protein interaction databases

BioGridi276517. 4 interactions.
MINTiMINT-4701389.
STRINGi4896.SPBC1539.09c-1.

Structurei

3D structure databases

ProteinModelPortaliQ92370.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 223197Glutamine amidotransferase type-1Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni257 – 519263Indole-3-glycerol phosphate synthaseAdd
BLAST
Regioni536 – 759224N-(5'-phosphoribosyl)anthranilate isomeraseAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0134.
HOGENOMiHOG000280459.
InParanoidiQ92370.
KOiK13501.
OMAiPVLGVCM.
OrthoDBiEOG78WM1Q.
PhylomeDBiQ92370.

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00134_B. IGPS_B.
MF_00135. PRAI.
InterProiIPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view]
PIRSFiPIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMiSSF51366. SSF51366. 3 hits.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92370-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKVDVGES VKGDASENAV KEVAERPIVM IDNYDSFTWN VVQYLSNLEK
60 70 80 90 100
RYPIMVFRND EITVDELEKL NPLKLVLSPG PGHPARDGGI CNEAISRFAG
110 120 130 140 150
KIPILGVCMG LQCIFETMGG KVDSAGEIIH GKVSKINHDG LGFYQGIPQN
160 170 180 190 200
ISVTRYHSLA GKISSLPDCL DVTSWTENGV IMGARHKKYA IEGVQYHPES
210 220 230 240 250
ILSEYGKEYI QNFLNLTAGT WEENGIVMPT KNNAFNAAMR ENSNSVSSTK
260 270 280 290 300
IRKQESILEK IHAQRLIDIA ESKRKPGLSV GDLQTYLNLN IAPPCINFYE
310 320 330 340 350
RLKQSKPALM AEVKRASPSK GDIKLDANAA IQALTYAQVG ASVISVLTEP
360 370 380 390 400
KWFKGSLNDL FVARKAVEHV ANRPAILRKD FIIDPYQIME ARLNGADSVL
410 420 430 440 450
LIVAMLSREQ LESLYKFSKS LGMEPLVEVN CAEEMKTAIE LGAKVIGVNN
460 470 480 490 500
RNLHSFEVDL STTSKLAEMV PDDVILAALS GISSPADVAH YSSQGVSAVL
510 520 530 540 550
VGESLMRASD PAAFARELLN LSSSEISNGK KTSTPLVKVC GTRSLLAAKT
560 570 580 590 600
IVESGGDLIG LIFVEKSKRK VDLSVAKEIS HFVHTTNRKH ISPKKAVTGQ
610 620 630 640 650
SWFDHQYENL ASSPHPLLVG VFQNQPLEYI RSIIAEVNLD IVQLHGQEPF
660 670 680 690 700
EWIHMLDRPV IKVFPLNSSE ISRPNYHIVP LIDAYVGGES GGLGKKVDWE
710 720 730 740 750
AASFIPVSYV LAGGLTPKNV QDAISVSRPA VVDVSSGVET DGKQDLEKIK

AFINAVKEL
Length:759
Mass (Da):83,040
Last modified:April 26, 2001 - v2
Checksum:i519E2EA55CB9B212
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti387 – 3871Q → E in CAA70348 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09137 mRNA. Translation: CAA70348.1.
CU329671 Genomic DNA. Translation: CAB51341.1.
PIRiT39468.
T46566.
RefSeqiNP_596823.1. NM_001023843.1.

Genome annotation databases

EnsemblFungiiSPBC1539.09c.1; SPBC1539.09c.1:pep; SPBC1539.09c.
GeneIDi2539973.
KEGGispo:SPBC1539.09c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09137 mRNA. Translation: CAA70348.1.
CU329671 Genomic DNA. Translation: CAB51341.1.
PIRiT39468.
T46566.
RefSeqiNP_596823.1. NM_001023843.1.

3D structure databases

ProteinModelPortaliQ92370.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276517. 4 interactions.
MINTiMINT-4701389.
STRINGi4896.SPBC1539.09c-1.

Protein family/group databases

MEROPSiC26.A25.

Proteomic databases

MaxQBiQ92370.
PaxDbiQ92370.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC1539.09c.1; SPBC1539.09c.1:pep; SPBC1539.09c.
GeneIDi2539973.
KEGGispo:SPBC1539.09c.

Organism-specific databases

PomBaseiSPBC1539.09c.

Phylogenomic databases

eggNOGiCOG0134.
HOGENOMiHOG000280459.
InParanoidiQ92370.
KOiK13501.
OMAiPVLGVCM.
OrthoDBiEOG78WM1Q.
PhylomeDBiQ92370.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
UPA00035; UER00042.
UPA00035; UER00043.

Miscellaneous databases

NextBioi20801116.

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00134_B. IGPS_B.
MF_00135. PRAI.
InterProiIPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view]
PIRSFiPIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMiSSF51366. SSF51366. 3 hits.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. David C., Couzin N., Lauquin G.J.-M.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiTRPG_SCHPO
AccessioniPrimary (citable) accession number: Q92370
Secondary accession number(s): Q9UUF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: April 26, 2001
Last modified: March 31, 2015
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.