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Protein

Helicase sen1

Gene

sen1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent 5'->3' DNA/RNA helicase required for the expression and maturation of diverse classes of non-protein-coding RNAs like precursor tRNAs, rRNAs and small nuclear (snRNA) and nucleolar (snoRNA) RNAs. Directs RNA polymerase II transcription termination on snoRNAs as well as on several short protein-coding genes. May also play a role in transcription-coupled nucleotide excision repair (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei1134 – 11341ATPBy similarity
Binding sitei1407 – 14071ATPBy similarity
Binding sitei1445 – 14451ATPBy similarity
Binding sitei1574 – 15741ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1155 – 11595ATPBy similarity

GO - Molecular functioni

  • ATP binding Source: PomBase
  • ATP-dependent 5'-3' DNA/RNA helicase activity Source: PomBase
  • ATP-dependent 5'-3' RNA helicase activity Source: PomBase
  • single-stranded DNA-dependent ATP-dependent DNA helicase activity Source: PomBase

GO - Biological processi

  • DNA duplex unwinding Source: GOC
  • mRNA 3'-end processing Source: PomBase
  • negative regulation of RNA polymerase III activity Source: PomBase
  • rRNA processing Source: PomBase
  • snoRNA processing Source: PomBase
  • snRNA processing Source: PomBase
  • termination of RNA polymerase II transcription Source: PomBase
  • tRNA processing Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, rRNA processing, tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Helicase sen1 (EC:3.6.4.-)
Gene namesi
Name:sen1
ORF Names:SPAC6G9.10c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC6G9.10c.
PomBaseiSPAC6G9.10c. sen1.

Subcellular locationi

GO - Cellular componenti

  • Nrd1 complex Source: PomBase
  • nuclear chromatin Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16871687Helicase sen1PRO_0000080723Add
BLAST

Proteomic databases

MaxQBiQ92355.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi278055. 20 interactions.
MINTiMINT-4701249.

Structurei

3D structure databases

ProteinModelPortaliQ92355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA2/NAM7 helicase family.Curated

Phylogenomic databases

InParanoidiQ92355.
KOiK10706.
OrthoDBiEOG70PC5T.
PhylomeDBiQ92355.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
InterProiIPR024481. Helicase_Sen1_N.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF12726. SEN1_N. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Q92355-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAENLSDQDC FEKLSSSKEG QHWFCSGLLT QYIQPTFFWF AHDETPSFKW
60 70 80 90 100
VLNAYHERLR SCTSCIQAYY ELRNESLAKG SYSFTGFSIT DLQEKWNKWD
110 120 130 140 150
IIRVLEDFKA LEDETIDFTS LPCLIFETLL NPKLFTCKNI YKNAIDAFNG
160 170 180 190 200
LLSDWCSNIF LPGYLLFFYE ASHPDVLEWV HSFFKENTEI RISSATVDAV
210 220 230 240 250
FNTVVFESQN SSDSDCNFVS ISSPEFWERT YMFLELLPLQ SITAACESIL
260 270 280 290 300
KDYLLNLVKT SESLSSQQMS CLRICCNSSS FWSAADSFKE VSSFLKNLLK
310 320 330 340 350
NVTATPFEVS DMNWAYIIAS FFRTCLNDFV SVFPEWLKGF VEEKRTIGFI
360 370 380 390 400
IYSVLDALFD LLSLDYSSPS FLNNTLSLMN ANSITILQDY PEYMLKLRLH
410 420 430 440 450
SLLYDIAFIS WSHKAITKNP SFVFDPSYEL SPFWKLDNLQ EEKISDVLFS
460 470 480 490 500
KISSCCFAHD IEISENSTPS GTMVQFAELW QVMSEYISGF LKGFSEKSST
510 520 530 540 550
EISNMLGDSS KFDTVVSFLL SPTQPLYVSA FHIVQIITNC TKNRNEALKK
560 570 580 590 600
LVAMDFRGIV HGLADAVLNW QSILSFFPAL RIMRFLSITN KSLSSDNSAF
610 620 630 640 650
TENDIPTLGA YWQCIWNILD LVFSNVARWS LNNPADTVKA LMKLTLKFVD
660 670 680 690 700
DLFQNDGIFI KLLAKFDSLI LLGETSESLF SFIMWLKIND LELRGIVINS
710 720 730 740 750
LCKLFTKFSN FDYLFEDRTV TFLTDFIIRK QKAHLSADQC KQLANVLTQA
760 770 780 790 800
SPEAKTVLEQ HRLSEMRKTK KQTELTNSAH VIKPSPTPQI TVKQNTTKSS
810 820 830 840 850
SAPRMGMLEQ LKQEYLTKRN FESKLKSSAV SSRKPTFNEV KPANLLAEDL
860 870 880 890 900
SDNEDDIDRK QGLFSLAKAN KIPEIRQQER RQVQLLSNST IKMHPSQIRM
910 920 930 940 950
MTNRNVANVK ARLFPSMTDF YKEILSWEPA NQSPNPVLKF HKLDGKIIDS
960 970 980 990 1000
FKTVEHYMEV LQPMIFMECW SQIQSTKLDL KFSPVEGIMV ERTAVNNFVD
1010 1020 1030 1040 1050
IGVSVAPKDL YGYPLYDTEV VSLAFNKEDA SSMKGLCCFA KVERIVRQTN
1060 1070 1080 1090 1100
GVLVVLRTLP SMEILNKLQG NCALWFLKLT NLATFTRQYA GIRGLPYFHL
1110 1120 1130 1140 1150
ADDIIRARPC SQPVKHSSSE IKAAMKRYQV NEPQAKAIMC ALDNNGFTLI
1160 1170 1180 1190 1200
QGPPGTGKTK TIIGIISALL VDLSRYHITR PNQQSKSTES KQQILLCAPS
1210 1220 1230 1240 1250
NAAVDEVLLR LKRGFLLENG EKYIPRVVRI GNPETINVSV RDLSLEYQTE
1260 1270 1280 1290 1300
KQLLEVNQGA IDLGSLQELT RWRDTFYDCI QKIEELEKQI DVARDVAEDT
1310 1320 1330 1340 1350
KSLGKELQNK INEKNLAEQK VEELQSQSFT KNKEVDLLRK KAQKAILKQA
1360 1370 1380 1390 1400
DVVCATLSGS GHDLVAHSSL NFSTVIIDEA AQAVELDTII PLRYGAKKCI
1410 1420 1430 1440 1450
LVGDPNQLPP TVLSKKAASL NYSQSLFVRI QKNFSNQMCL LSIQYRMHPD
1460 1470 1480 1490 1500
ISHFPSKKFY DSRLEDGDNM AEKTQQVWHV NPKFTQYRLF DVRGKERTSN
1510 1520 1530 1540 1550
TMSTYNLEEV EYLVNMVDEL LNKFPDVNFT GRIGVITPYR SQLHELRRAF
1560 1570 1580 1590 1600
KVKYGKSFMS TIDIQTVDGF QGQEKDIIFF SCVKSYSKHG IGFLRDFRRL
1610 1620 1630 1640 1650
NVALTRARSS LLIIGNMETL KTDDLWGSLV DDALSRKLVE SPHIDSEGRL
1660 1670 1680
ITISRTSEKR MKNEEFVEPP SKKLANSEPS KEIRQRS
Length:1,687
Mass (Da):192,548
Last modified:February 1, 1997 - v1
Checksum:i92C82F2049E2680D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB03612.1.
PIRiT39072.
RefSeqiNP_594119.1. NM_001019543.2.

Genome annotation databases

EnsemblFungiiSPAC6G9.10c.1; SPAC6G9.10c.1:pep; SPAC6G9.10c.
GeneIDi2541556.
KEGGispo:SPAC6G9.10c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB03612.1.
PIRiT39072.
RefSeqiNP_594119.1. NM_001019543.2.

3D structure databases

ProteinModelPortaliQ92355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278055. 20 interactions.
MINTiMINT-4701249.

Proteomic databases

MaxQBiQ92355.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC6G9.10c.1; SPAC6G9.10c.1:pep; SPAC6G9.10c.
GeneIDi2541556.
KEGGispo:SPAC6G9.10c.

Organism-specific databases

EuPathDBiFungiDB:SPAC6G9.10c.
PomBaseiSPAC6G9.10c. sen1.

Phylogenomic databases

InParanoidiQ92355.
KOiK10706.
OrthoDBiEOG70PC5T.
PhylomeDBiQ92355.

Miscellaneous databases

NextBioi20802652.
PROiQ92355.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
InterProiIPR024481. Helicase_Sen1_N.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF12726. SEN1_N. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "The sen1(+) gene of Schizosaccharomyces pombe, a homologue of budding yeast SEN1, encodes an RNA and DNA helicase."
    Kim H.-D., Choe J., Seo Y.-S.
    Biochemistry 38:14697-14710(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1230-1241 AND 1489-1493, FUNCTION, SUBUNIT.
    Strain: 972 / ATCC 24843.
  3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSEN1_SCHPO
AccessioniPrimary (citable) accession number: Q92355
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: December 9, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.