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Q92331 (VPS5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Vacuolar protein sorting-associated protein 5
Alternative name(s):
Carboxypeptidase Y-deficient protein 10
Vacuolar protein-targeting protein 5
Gene names
Name:VPS5
Synonyms:GRD2, PEP10, VPT5
Ordered Locus Names:YOR069W
ORF Names:YOR29-20
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length675 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in vesicular protein sorting. Required for retention of late Golgi membrane proteins and vacuolar biogenesis. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. The VPS5-VPS17 subcomplex may assemble onto the membrane to promote vesicle formation.

Subunit structure

Component of the retromer complex which consists of VPS29, VPS26, VPS35, VPS5 and VPS17. Component of a retromer subcomplex consisting of VPSD5 and VPS17.

Subcellular location

Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side Potential. Endosome membrane; Peripheral membrane protein; Cytoplasmic side Potential.

Domain

The PX domain binds phosphatidylinositol 3-phosphate which is necessary for peripheral membrane localization By similarity.

Post-translational modification

Phosphorylated on serine residue(s). Ref.9 Ref.10

Miscellaneous

Present with 6120 molecules/cell in log phase SD medium. Ref.8

Sequence similarities

Belongs to the sorting nexin family.

Contains 1 PX (phox homology) domain.

Sequence caution

The sequence CAA94554.1 differs from that shown. Reason: Frameshift at position 13.

The sequence CAA99262.1 differs from that shown. Reason: Erroneous initiation.

The sequence Z74976 differs from that shown. Reason: Frameshift at position 13.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

VPS17P329137EBI-20483,EBI-20366
VPS35P341103EBI-20483,EBI-20415

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 675675Vacuolar protein sorting-associated protein 5
PRO_0000213804

Regions

Domain279 – 394116PX

Sites

Binding site3201Phosphatidylinositol 3-phosphate By similarity
Binding site3461Phosphatidylinositol 3-phosphate By similarity
Binding site3601Phosphatidylinositol 3-phosphate By similarity

Amino acid modifications

Modified residue371Phosphoserine Ref.10
Modified residue1421Phosphoserine Ref.10
Modified residue1841Phosphoserine Ref.9
Modified residue2521Phosphoserine Ref.9

Experimental info

Sequence conflict3761V → A in AAS56361. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Q92331 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: FBBA994EADBDC2BD

FASTA67576,484
        10         20         30         40         50         60 
MDYEDNLEAP VWDELNHEGD KTQSLVSNSI ESIGQISTNE ERKDNELLET TASFADKIDL 

        70         80         90        100        110        120 
DSAPEWKDPG LSVAGNPQLE EHDNSKADDL INSLAPEQDP IADLKNSTTQ FIATRESGGA 

       130        140        150        160        170        180 
LFTGNANSPL VFDDTIYDAN TSPNTSKSIS GRRSGKPRIL FDSARAQRNS KRNHSLKAKR 

       190        200        210        220        230        240 
TTASDDTIKT PFTDPLKKAE KENEFVEEPL DDRNERRENN EGKFTASVEK NILEQVDRPL 

       250        260        270        280        290        300 
YNLPQTGANI SSPAEVEENS EKFGKTKIGS KVPPTEKAVA FKVEVKDPVK VGELTSIHVE 

       310        320        330        340        350        360 
YTVISESSLL ELKYAQVSRR YRDFRWLYRQ LQNNHWGKVI PPPPEKQSVG SFKENFIENR 

       370        380        390        400        410        420 
RFQMESMLKK ICQDPVLQKD KDFLLFLTSD DFSSESKKRA FLTGSGAIND SNDLSEVRIS 

       430        440        450        460        470        480 
EIQLLGAEDA AEVLKNGGID AESHKGFMSI SFSSLPKYNE ADEFFIEKKQ KIDELEDNLK 

       490        500        510        520        530        540 
KLSKSLEMVD TSRNTLAAST EEFSSMVETL ASLNVSEPNS ELLNNFADVH KSIKSSLERS 

       550        560        570        580        590        600 
SLQETLTMGV MLDDYIRSLA SVKAIFNQRS KLGYFLVVIE NDMNKKHSQL GKLGQNIHSE 

       610        620        630        640        650        660 
KFREMRKEFQ TLERRYNLTK KQWQAVGDKI KDEFQGFSTD KIREFRNGME ISLEAAIESQ 

       670 
KECIELWETF YQTNL 

« Hide

References

« Hide 'large scale' references
[1]"The yeast VPS5/GRD2 gene encodes a sorting nexin-1-like protein required for localizing membrane proteins to the late Golgi."
Nothwehr S.F., Hindes A.E.
J. Cell Sci. 110:1063-1072(1997) [PubMed: 9175702] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A sorting nexin-1 homologue, Vps5p, forms a complex with Vps17p and is required for recycling the vacuolar protein-sorting receptor."
Horazdovsky B.F., Davies B.A., Seaman M.N.J., McLaughlin S.A., Yoon S., Emr S.D.
Mol. Biol. Cell 8:1529-1541(1997) [PubMed: 9285823] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames."
Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M.
Yeast 13:379-390(1997) [PubMed: 9133743] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed: 9169874] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 364-675.
Strain: ATCC 204508 / S288c.
[7]"A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast."
Seaman M.N., McCaffery J.M., Emr S.D.
J. Cell Biol. 142:665-681(1998) [PubMed: 9700157] [Abstract]
Cited for: IDENTIFICATION IN THE RETROMER COMPLEX.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-184 AND SER-252, MASS SPECTROMETRY.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37 AND SER-142, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U73512 Genomic DNA. Translation: AAB62976.1.
U84735 Genomic DNA. Translation: AAB41798.1.
Z70678 Genomic DNA. Translation: CAA94554.1. Frameshift.
Z74976 Genomic DNA. No translation available.
Z74977 Genomic DNA. Translation: CAA99262.1. Different initiation.
AY558035 Genomic DNA. Translation: AAS56361.1.
BK006948 Genomic DNA. Translation: DAA10847.1.
PIRS66952.
RefSeqNP_014712.2. NM_001183488.1.

3D structure databases

ProteinModelPortalQ92331.
SMRQ92331. Positions 279-391.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-1740N.
IntActQ92331. 44 interactions.
MINTMINT-391689.
STRINGQ92331.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR069W; YOR069W; YOR069W.
GeneID854235.
KEGGsce:YOR069W.
NMPDRfig|4932.3.peg.5811.

Organism-specific databases

CYGDYOR069w.
SGDS000005595. VPS5.

Phylogenomic databases

eggNOGfuNOG05443.
GeneTreeEFGT00050000002717.
HOGENOMHBG202827.
OrthoDBEOG4W11NH.

Gene expression databases

ArrayExpressQ92331.
GenevestigatorQ92331.
GermOnlineYOR069W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR001683. Phox.
IPR015404. Vps5_C.
[Graphical view]
Gene3DG3DSA:3.30.1520.10. PX. 1 hit.
PfamPF00787. PX. 1 hit.
PF09325. Vps5. 1 hit.
[Graphical view]
SMARTSM00312. PX. 1 hit.
[Graphical view]
SUPFAMSSF64268. PX. 1 hit.
PROSITEPS50195. PX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio976127.

Entry information

Entry nameVPS5_YEAST
AccessionPrimary (citable) accession number: Q92331
Secondary accession number(s): D6W2D1, E9P8U6, Q08483
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: December 14, 2011
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families