Vacuolar protein sorting-associated protein 5 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot Q92331 (VPS5_YEAST)

Last modified December 15, 2009. Version 81. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Vacuolar protein sorting-associated protein 5
Alternative name(s):
    Vacuolar protein-targeting protein 5
    Carboxypeptidase Y-deficient protein 10

Gene names

Name:	VPS5
Synonyms:	GRD2, PEP10, VPT5
Ordered Locus Names:	YOR069W
ORF Names:	YOR29-20

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	675 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Plays a role in vesicular protein sorting. Required for retention of late Golgi membrane proteins and vacuolar biogenesis. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. The VPS5-VPS17 subcomplex may assemble onto the membrane to promote vesicle formation.
Subunit structure	Component of the retromer complex which consists of VPS29, VPS26, VPS35, VPS5 and VPS17. Component of a retromer subcomplex consisting of VPSD5 and VPS17.
Subcellular location	Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side Potential. Endosome membrane; Peripheral membrane protein; Cytoplasmic side Potential.
Post-translational modification	Phosphorylated on serine residue(s). Ref.7 Ref.8
Miscellaneous	Present with 6120 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the sorting nexin family. Contains 1 PX (phox homology) domain.
Sequence caution	The sequence CAA94554.1 differs from that shown. Reason: Frameshift at position 13. The sequence Z74976 differs from that shown. Reason: Frameshift at position 13.

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Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Endosome Golgi apparatus Membrane
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell communication Inferred from electronic annotation. Source: InterPro protein retention in Golgi apparatus Inferred from mutant phenotype. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW retrograde transport, endosome to Golgi Ref.5 Inferred from physical interaction. Source: SGD
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW cytosol Ref.1 Inferred from direct assay. Source: SGD endosome Ref.1 Ref.5 Inferred from direct assay. Source: SGD retromer complex, outer shell Ref.5 Inferred from physical interaction. Source: SGD
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct phosphatidylinositol 3-phosphate binding Inferred from direct assay. Source: SGD
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
itself		1	EBI-20483,EBI-20483
	P48232	1	EBI-20483,EBI-28775
	Q03687	1	EBI-20483,EBI-28024
ATG27	P46989	1	EBI-20483,EBI-26164
PEP1	P32319	1	EBI-20483,EBI-13123
SNA4	Q07549	1	EBI-20483,EBI-22078
TVP15	Q03860	1	EBI-20483,EBI-37537
VPS17	P32913	3	EBI-20483,EBI-20366
VPS26	P40335	1	EBI-20483,EBI-20373
VPS29	P38759	1	EBI-20483,EBI-13092
VPS35	P34110	1	EBI-20483,EBI-20415
YIF1	P53845	1	EBI-20483,EBI-28230

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 675

675

Vacuolar protein sorting-associated protein 5

PRO_0000213804

Regions

Domain

279 – 394

116

Amino acid modifications

Modified residue

Phosphoserine Ref.8

Modified residue

142

Phosphoserine Ref.8

Modified residue

184

Phosphoserine Ref.7

Modified residue

252

Phosphoserine Ref.7

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Sequences

Sequence

Length

Mass (Da)

Tools

Q92331-1 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: FBBA994EADBDC2BD
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FASTA

675

76,484

        10         20         30         40         50         60 
MDYEDNLEAP VWDELNHEGD KTQSLVSNSI ESIGQISTNE ERKDNELLET TASFADKIDL 

        70         80         90        100        110        120 
DSAPEWKDPG LSVAGNPQLE EHDNSKADDL INSLAPEQDP IADLKNSTTQ FIATRESGGA 

       130        140        150        160        170        180 
LFTGNANSPL VFDDTIYDAN TSPNTSKSIS GRRSGKPRIL FDSARAQRNS KRNHSLKAKR 

       190        200        210        220        230        240 
TTASDDTIKT PFTDPLKKAE KENEFVEEPL DDRNERRENN EGKFTASVEK NILEQVDRPL 

       250        260        270        280        290        300 
YNLPQTGANI SSPAEVEENS EKFGKTKIGS KVPPTEKAVA FKVEVKDPVK VGELTSIHVE 

       310        320        330        340        350        360 
YTVISESSLL ELKYAQVSRR YRDFRWLYRQ LQNNHWGKVI PPPPEKQSVG SFKENFIENR 

       370        380        390        400        410        420 
RFQMESMLKK ICQDPVLQKD KDFLLFLTSD DFSSESKKRA FLTGSGAIND SNDLSEVRIS 

       430        440        450        460        470        480 
EIQLLGAEDA AEVLKNGGID AESHKGFMSI SFSSLPKYNE ADEFFIEKKQ KIDELEDNLK 

       490        500        510        520        530        540 
KLSKSLEMVD TSRNTLAAST EEFSSMVETL ASLNVSEPNS ELLNNFADVH KSIKSSLERS 

       550        560        570        580        590        600 
SLQETLTMGV MLDDYIRSLA SVKAIFNQRS KLGYFLVVIE NDMNKKHSQL GKLGQNIHSE 

       610        620        630        640        650        660 
KFREMRKEFQ TLERRYNLTK KQWQAVGDKI KDEFQGFSTD KIREFRNGME ISLEAAIESQ 

       670 
KECIELWETF YQTNL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The yeast VPS5/GRD2 gene encodes a sorting nexin-1-like protein required for localizing membrane proteins to the late Golgi." Nothwehr S.F., Hindes A.E. J. Cell Sci. 110:1063-1072(1997) [PubMed: 9175702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A sorting nexin-1 homologue, Vps5p, forms a complex with Vps17p and is required for recycling the vacuolar protein-sorting receptor." Horazdovsky B.F., Davies B.A., Seaman M.N.J., McLaughlin S.A., Yoon S., Emr S.D. Mol. Biol. Cell 8:1529-1541(1997) [PubMed: 9285823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames." Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M. Yeast 13:379-390(1997) [PubMed: 9133743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast." Seaman M.N., McCaffery J.M., Emr S.D. J. Cell Biol. 142:665-681(1998) [PubMed: 9700157] [Abstract] Cited for: IDENTIFICATION IN THE RETROMER COMPLEX.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-184 AND SER-252, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37 AND SER-142, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	U73512 Genomic DNA. Translation: AAB62976.1. U84735 Genomic DNA. Translation: AAB41798.1. Z70678 Genomic DNA. Translation: CAA94554.1. Frameshift. Z74976 Genomic DNA. No translation available. Z74977 Genomic DNA. Translation: CAA99262.1. Different initiation.
PIR	S66952.
RefSeq	NP_014712.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1740N.
IntAct	Q92331. 44 interactions.
STRING	Q92331.
Genome annotation databases
Ensembl	YOR069W; YOR069W; YOR069W; Saccharomyces cerevisiae. [Genome view]
GeneID	854235.
KEGG	sce:YOR069W.
NMPDR	fig\|4932.3.peg.5811.
Organism-specific databases
CYGD	YOR069w.
SGD	S000005595. VPS5.
Phylogenomic databases
HOGENOM	HBG202827.
OrthoDB	EOG96MD1R.
Gene expression databases
ArrayExpress	Q92331.
Genevestigator	Q92331.
GermOnline	YOR069W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001683. PX. IPR015404. Vps5_C. [Graphical view]
Gene3D	G3DSA:3.30.1520.10. PX. 1 hit.
Pfam	PF00787. PX. 1 hit. PF09325. Vps5. 1 hit. [Graphical view]
SMART	SM00312. PX. 1 hit. [Graphical view]
PROSITE	PS50195. PX. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	976127.

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Entry information

Entry name

VPS5_YEAST

Accession

Primary (citable) accession number: Q92331
Secondary accession number(s): Q08483

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	December 15, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents