Reviewed,
UniProtKB/Swiss-Prot Q92331 (VPS5_YEAST)
Last modified
December 15, 2009.
Version 81.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Vacuolar protein sorting-associated protein 5 Alternative name(s): Vacuolar protein-targeting protein 5 Carboxypeptidase Y-deficient protein 10 | ||||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 675 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Plays a role in vesicular protein sorting. Required for retention of late Golgi membrane proteins and vacuolar biogenesis. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. The VPS5-VPS17 subcomplex may assemble onto the membrane to promote vesicle formation. |
| Subunit structure | Component of the retromer complex which consists of VPS29, VPS26, VPS35, VPS5 and VPS17. Component of a retromer subcomplex consisting of VPSD5 and VPS17. |
| Subcellular location | Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side Potential. Endosome membrane; Peripheral membrane protein; Cytoplasmic side Potential. |
| Post-translational modification | |
| Miscellaneous | Present with 6120 molecules/cell in log phase SD medium. Ref.6 |
| Sequence similarities | Belongs to the sorting nexin family. Contains 1 PX (phox homology) domain. |
| Sequence caution | The sequence CAA94554.1 differs from that shown. Reason: Frameshift at position 13. The sequence Z74976 differs from that shown. Reason: Frameshift at position 13. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Protein transport Transport |
| Cellular component | Cytoplasm Endosome Golgi apparatus Membrane |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | cell communication Inferred from electronic annotation. Source: InterPro protein retention in Golgi apparatusInferred from mutant phenotype. Source: SGD protein transportInferred from electronic annotation. Source: UniProtKB-KW retrograde transport, endosome to Golgi Ref.5Inferred from physical interaction. Source: SGD |
| Cellular component | Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW cytosol Ref.1Inferred from direct assay. Source: SGD endosome Ref.1 Ref.5Inferred from direct assay. Source: SGD retromer complex, outer shell Ref.5Inferred from physical interaction. Source: SGD |
| Molecular function | identical protein binding Inferred from physical interaction. Source: IntAct phosphatidylinositol 3-phosphate bindingInferred from direct assay. Source: SGD |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| itself | 1 | EBI-20483,EBI-20483 | ||
| P48232 | 1 | EBI-20483,EBI-28775 | ||
| Q03687 | 1 | EBI-20483,EBI-28024 | ||
| ATG27 | P46989 | 1 | EBI-20483,EBI-26164 | |
| PEP1 | P32319 | 1 | EBI-20483,EBI-13123 | |
| SNA4 | Q07549 | 1 | EBI-20483,EBI-22078 | |
| TVP15 | Q03860 | 1 | EBI-20483,EBI-37537 | |
| VPS17 | P32913 | 3 | EBI-20483,EBI-20366 | |
| VPS26 | P40335 | 1 | EBI-20483,EBI-20373 | |
| VPS29 | P38759 | 1 | EBI-20483,EBI-13092 | |
| VPS35 | P34110 | 1 | EBI-20483,EBI-20415 | |
| YIF1 | P53845 | 1 | EBI-20483,EBI-28230 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 675 | 675 | Vacuolar protein sorting-associated protein 5 | PRO_0000213804 | |||||
Regions | |||||||||
| Domain | 279 – 394 | 116 | PX | ||||||
Amino acid modifications | |||||||||
| Modified residue | 37 | 1 | Phosphoserine Ref.8 | ||||||
| Modified residue | 142 | 1 | Phosphoserine Ref.8 | ||||||
| Modified residue | 184 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 252 | 1 | Phosphoserine Ref.7 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The yeast VPS5/GRD2 gene encodes a sorting nexin-1-like protein required for localizing membrane proteins to the late Golgi." Nothwehr S.F., Hindes A.E. J. Cell Sci. 110:1063-1072(1997) [PubMed: 9175702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "A sorting nexin-1 homologue, Vps5p, forms a complex with Vps17p and is required for recycling the vacuolar protein-sorting receptor." Horazdovsky B.F., Davies B.A., Seaman M.N.J., McLaughlin S.A., Yoon S., Emr S.D. Mol. Biol. Cell 8:1529-1541(1997) [PubMed: 9285823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames." Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M. Yeast 13:379-390(1997) [PubMed: 9133743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [4] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K.Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [5] | "A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast." Seaman M.N., McCaffery J.M., Emr S.D. J. Cell Biol. 142:665-681(1998) [PubMed: 9700157] [Abstract] Cited for: IDENTIFICATION IN THE RETROMER COMPLEX. |
| [6] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [7] | "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-184 AND SER-252, MASS SPECTROMETRY. |
| [8] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37 AND SER-142, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U73512 Genomic DNA. Translation: AAB62976.1. U84735 Genomic DNA. Translation: AAB41798.1. Z70678 Genomic DNA. Translation: CAA94554.1. Frameshift. Z74976 Genomic DNA. No translation available. Z74977 Genomic DNA. Translation: CAA99262.1. Different initiation. | |
| PIR | S66952. |
| RefSeq | NP_014712.2. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-1740N. |
| IntAct | Q92331. 44 interactions. |
| STRING | Q92331. |
Genome annotation databases | |
| Ensembl | YOR069W; YOR069W; YOR069W; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 854235. |
| KEGG | sce:YOR069W. |
| NMPDR | fig|4932.3.peg.5811. |
Organism-specific databases | |
| CYGD | YOR069w. |
| SGD | S000005595. VPS5. |
Phylogenomic databases | |
| HOGENOM | HBG202827. |
| OrthoDB | EOG96MD1R. |
Gene expression databases | |
| ArrayExpress | Q92331. |
| Genevestigator | Q92331. |
| GermOnline | YOR069W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR001683. PX. IPR015404. Vps5_C. [Graphical view] |
| Gene3D | G3DSA:3.30.1520.10. PX. 1 hit. |
| Pfam | PF00787. PX. 1 hit. PF09325. Vps5. 1 hit. [Graphical view] |
| SMART | SM00312. PX. 1 hit. [Graphical view] |
| PROSITE | PS50195. PX. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 976127. |
Entry information
| Entry name | VPS5_YEAST | ||||||||
| Accession | Primary (citable) accession number: Q92331 Secondary accession number(s): Q08483 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |

Clusters with


