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Protein

UBX domain-containing protein 1

Gene

Ubxn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-binding protein that plays a role in the modulation of innate immune response. Blocks both the RIG-I-like receptors (RLR) and NF-kappa-B pathways. Following viral infection, UBXN1 is induced and recruited to the RLR component MAVS. In turn, interferes with MAVS oligomerization, and disrupts the MAVS/TRAF3/TRAF6 signalosome. This function probably serves as a brake to prevent excessive RLR signaling. Interferes with the TNFalpha-triggered NF-kappa-B pathway by interacting with cellular inhibitors of apoptosis proteins (cIAPs) and thereby inhibiting their recruitment to TNFR1. Prevents also the activation of NF-kappa-B by associating with CUL1 and thus inhibiting NF-kappa-B inhibitor alpha/NFKBIA degradation that remains bound to NF-kappa-B. Interacts with the BRCA1-BARD1 heterodimer and regulates its activity. Specifically binds 'Lys-6'-linked polyubiquitin chains. Interaction with autoubiquitinated BRCA1 leads to the inhibition of the E3 ubiquitin-protein ligase activity of the BRCA1-BARD1 heterodimer. Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-532668 N-glycan trimming in the ER and Calnexin/Calreticulin cycle

Protein family/group databases

MEROPSiC19.089

Names & Taxonomyi

Protein namesi
Recommended name:
UBX domain-containing protein 1
Alternative name(s):
Protein 2B28
SAPK substrate protein 1
UBA/UBX 33.3 kDa protein
Short name:
mY33K
Gene namesi
Name:Ubxn1
Synonyms:D19Ertd721e, Saks1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1289301 Ubxn1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002110242 – 297UBX domain-containing protein 1Add BLAST296

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei199PhosphoserineCombined sources1
Modified residuei200Phosphoserine; by MAPK12By similarity1
Modified residuei207PhosphothreonineBy similarity1
Modified residuei229PhosphothreonineBy similarity1
Modified residuei270PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ922Y1
MaxQBiQ922Y1
PaxDbiQ922Y1
PeptideAtlasiQ922Y1
PRIDEiQ922Y1

2D gel databases

REPRODUCTION-2DPAGEIPI00123589
Q922Y1

PTM databases

iPTMnetiQ922Y1
PhosphoSitePlusiQ922Y1

Expressioni

Gene expression databases

BgeeiENSMUSG00000071655
GenevisibleiQ922Y1 MM

Interactioni

Subunit structurei

Interacts with MAVS; this interaction prevents MAVS oligomerization and thus disrupts the RLR signaling pathway. Interacts with CUL1; this interaction inhibits CUL1-mediated degradation of NF-kappa-B inhibitors. Interacts with BIRC2/c-IAP1; this interaction prevents TNFalpha-stimulated RIP1 ubiquitination and subsequent NF-kappa-B activation. Component of a complex required to couple retrotranslocation, ubiquitination and deglycosylation composed of NGLY1, SAKS1, AMFR, VCP and RAD23B (PubMed:11562482). Interacts with HOMER2 (PubMed:16709668). Interacts directly with VCP. Interacts with BRCA1 and BARD1; interaction takes place when BRCA1 is not autoubiquitinated but is strongly enhanced in the presence of autoubiquitinated BRCA1.By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi230443, 2 interactors
IntActiQ922Y1, 4 interactors
MINTiQ922Y1
STRINGi10090.ENSMUSP00000093974

Structurei

Secondary structure

1297
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 11Combined sources6
Turni12 – 14Combined sources3
Helixi17 – 27Combined sources11
Helixi32 – 42Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WHCNMR-A2-52[»]
ProteinModelPortaliQ922Y1
SMRiQ922Y1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ922Y1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 42UBAPROSITE-ProRule annotationAdd BLAST41
Domaini209 – 291UBXPROSITE-ProRule annotationAdd BLAST83

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni43 – 297Interaction with BRCA1By similarityAdd BLAST255

Domaini

The UBA domain specifically recognizes and binds 'Lys-6'-linked polyubiquitin chains.By similarity

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2689 Eukaryota
ENOG4111HH6 LUCA
GeneTreeiENSGT00530000063500
HOGENOMiHOG000188321
HOVERGENiHBG082310
InParanoidiQ922Y1
OMAiPSAVITM
OrthoDBiEOG091G0N23
PhylomeDBiQ922Y1
TreeFamiTF313944

Family and domain databases

InterProiView protein in InterPro
IPR015940 UBA
IPR009060 UBA-like_sf
IPR029071 Ubiquitin-like_domsf
IPR001012 UBX_dom
PfamiView protein in Pfam
PF00627 UBA, 1 hit
PF00789 UBX, 1 hit
SMARTiView protein in SMART
SM00165 UBA, 1 hit
SM00166 UBX, 1 hit
SUPFAMiSSF46934 SSF46934, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50030 UBA, 1 hit
PS50033 UBX, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q922Y1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELTALESL IEMGFPRGRA EKALALTGNQ GIEAAMDWLM EHEDDPDVDE
60 70 80 90 100
PLETPLSHVL GREPTPSEQV GPEGSGSAAG ESRPILTEEE RQEQTKRMLE
110 120 130 140 150
LVAQKQRERE EREEREALER EKQRRRQGQE LSVARQKLQE DEMRRAAEER
160 170 180 190 200
RREKAEELAA RQRVREKIER DKAERAKKYG GSVGSRSSPP ATDPGPVPSS
210 220 230 240 250
PSQEPPTKRE YDQCRIQVRL PDGTSLTQTF RAREQLAAVR LYVELHRGEE
260 270 280 290
PGQDQDPVQL LSGFPRRAFS EADMERPLQE LGLVPSAVLI VAKKCPS
Length:297
Mass (Da):33,573
Last modified:December 1, 2001 - v1
Checksum:i50746EF6CF340269
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK150442 mRNA Translation: BAE29564.1
BC006701 mRNA Translation: AAH06701.1
CCDSiCCDS29553.1
RefSeqiNP_666205.1, NM_146093.1
XP_006526985.1, XM_006526922.1
UniGeneiMm.27839

Genome annotation databases

EnsembliENSMUST00000096255; ENSMUSP00000093974; ENSMUSG00000071655
ENSMUST00000166407; ENSMUSP00000133250; ENSMUSG00000071655
GeneIDi225896
KEGGimmu:225896
UCSCiuc008gnp.1 mouse

Similar proteinsi

Entry informationi

Entry nameiUBXN1_MOUSE
AccessioniPrimary (citable) accession number: Q922Y1
Secondary accession number(s): Q3UCP8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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