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Protein

UBX domain-containing protein 1

Gene

Ubxn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-binding protein that interacts with the BRCA1-BARD1 heterodimer, and regulates its activity. Specifically binds 'Lys-6'-linked polyubiquitin chains. Interaction with autoubiquitinated BRCA1, leads to inhibit the E3 ubiquitin-protein ligase activity of the BRCA1-BARD1 heterodimer (By similarity) Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol. Involved in ubiquitin-proteasome systems.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-532668. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.

Protein family/group databases

MEROPSiC19.089.

Names & Taxonomyi

Protein namesi
Recommended name:
UBX domain-containing protein 1
Alternative name(s):
Protein 2B28
SAPK substrate protein 1
UBA/UBX 33.3 kDa protein
Short name:
mY33K
Gene namesi
Name:Ubxn1
Synonyms:D19Ertd721e, Saks1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1289301. Ubxn1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002110242 – 297UBX domain-containing protein 1Add BLAST296

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei199PhosphoserineCombined sources1
Modified residuei200Phosphoserine; by MAPK12By similarity1
Modified residuei207PhosphothreonineBy similarity1
Modified residuei229PhosphothreonineBy similarity1
Modified residuei270PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ922Y1.
MaxQBiQ922Y1.
PaxDbiQ922Y1.
PeptideAtlasiQ922Y1.
PRIDEiQ922Y1.

2D gel databases

REPRODUCTION-2DPAGEIPI00123589.
Q922Y1.

PTM databases

iPTMnetiQ922Y1.
PhosphoSitePlusiQ922Y1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000071655.
GenevisibleiQ922Y1. MM.

Interactioni

Subunit structurei

Interacts with BRCA1 and BARD1; interaction takes place when BRCA1 is not autoubiquitinated bur is strongly enhanced in the presence of autoubiquitinated BRCA1 (By similarity). Component of a complex required to couple retrotranslocation, ubiquitination and deglycosylation composed of NGLY1, SAKS1, AMFR, VCP and RAD23B. Interacts with HOMER2. Interacts directly with VCP.By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ922Y1. 4 interactors.
MINTiMINT-1342018.
STRINGi10090.ENSMUSP00000093974.

Structurei

Secondary structure

1297
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 11Combined sources6
Turni12 – 14Combined sources3
Helixi17 – 27Combined sources11
Helixi32 – 42Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WHCNMR-A2-52[»]
ProteinModelPortaliQ922Y1.
SMRiQ922Y1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ922Y1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 42UBAPROSITE-ProRule annotationAdd BLAST41
Domaini209 – 291UBXPROSITE-ProRule annotationAdd BLAST83

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni43 – 297Interaction with BRCA1By similarityAdd BLAST255

Domaini

The UBA domain specifically recognizes and binds 'Lys-6'-linked polyubiquitin chains.By similarity

Sequence similaritiesi

Contains 1 UBA domain.PROSITE-ProRule annotation
Contains 1 UBX domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2689. Eukaryota.
ENOG4111HH6. LUCA.
GeneTreeiENSGT00530000063500.
HOGENOMiHOG000188321.
HOVERGENiHBG082310.
InParanoidiQ922Y1.
OMAiHTEVEYH.
OrthoDBiEOG091G0N23.
PhylomeDBiQ922Y1.
TreeFamiTF313944.

Family and domain databases

InterProiIPR015940. UBA.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
IPR001012. UBX_dom.
[Graphical view]
PfamiPF00627. UBA. 1 hit.
PF00789. UBX. 1 hit.
[Graphical view]
SMARTiSM00165. UBA. 1 hit.
SM00166. UBX. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50030. UBA. 1 hit.
PS50033. UBX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q922Y1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELTALESL IEMGFPRGRA EKALALTGNQ GIEAAMDWLM EHEDDPDVDE
60 70 80 90 100
PLETPLSHVL GREPTPSEQV GPEGSGSAAG ESRPILTEEE RQEQTKRMLE
110 120 130 140 150
LVAQKQRERE EREEREALER EKQRRRQGQE LSVARQKLQE DEMRRAAEER
160 170 180 190 200
RREKAEELAA RQRVREKIER DKAERAKKYG GSVGSRSSPP ATDPGPVPSS
210 220 230 240 250
PSQEPPTKRE YDQCRIQVRL PDGTSLTQTF RAREQLAAVR LYVELHRGEE
260 270 280 290
PGQDQDPVQL LSGFPRRAFS EADMERPLQE LGLVPSAVLI VAKKCPS
Length:297
Mass (Da):33,573
Last modified:December 1, 2001 - v1
Checksum:i50746EF6CF340269
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK150442 mRNA. Translation: BAE29564.1.
BC006701 mRNA. Translation: AAH06701.1.
CCDSiCCDS29553.1.
RefSeqiNP_666205.1. NM_146093.1.
XP_006526985.1. XM_006526922.1.
UniGeneiMm.27839.

Genome annotation databases

EnsembliENSMUST00000096255; ENSMUSP00000093974; ENSMUSG00000071655.
ENSMUST00000166407; ENSMUSP00000133250; ENSMUSG00000071655.
GeneIDi225896.
KEGGimmu:225896.
UCSCiuc008gnp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK150442 mRNA. Translation: BAE29564.1.
BC006701 mRNA. Translation: AAH06701.1.
CCDSiCCDS29553.1.
RefSeqiNP_666205.1. NM_146093.1.
XP_006526985.1. XM_006526922.1.
UniGeneiMm.27839.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WHCNMR-A2-52[»]
ProteinModelPortaliQ922Y1.
SMRiQ922Y1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ922Y1. 4 interactors.
MINTiMINT-1342018.
STRINGi10090.ENSMUSP00000093974.

Protein family/group databases

MEROPSiC19.089.

PTM databases

iPTMnetiQ922Y1.
PhosphoSitePlusiQ922Y1.

2D gel databases

REPRODUCTION-2DPAGEIPI00123589.
Q922Y1.

Proteomic databases

EPDiQ922Y1.
MaxQBiQ922Y1.
PaxDbiQ922Y1.
PeptideAtlasiQ922Y1.
PRIDEiQ922Y1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000096255; ENSMUSP00000093974; ENSMUSG00000071655.
ENSMUST00000166407; ENSMUSP00000133250; ENSMUSG00000071655.
GeneIDi225896.
KEGGimmu:225896.
UCSCiuc008gnp.1. mouse.

Organism-specific databases

CTDi51035.
MGIiMGI:1289301. Ubxn1.

Phylogenomic databases

eggNOGiKOG2689. Eukaryota.
ENOG4111HH6. LUCA.
GeneTreeiENSGT00530000063500.
HOGENOMiHOG000188321.
HOVERGENiHBG082310.
InParanoidiQ922Y1.
OMAiHTEVEYH.
OrthoDBiEOG091G0N23.
PhylomeDBiQ922Y1.
TreeFamiTF313944.

Enzyme and pathway databases

ReactomeiR-MMU-532668. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.

Miscellaneous databases

EvolutionaryTraceiQ922Y1.
PROiQ922Y1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000071655.
GenevisibleiQ922Y1. MM.

Family and domain databases

InterProiIPR015940. UBA.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
IPR001012. UBX_dom.
[Graphical view]
PfamiPF00627. UBA. 1 hit.
PF00789. UBX. 1 hit.
[Graphical view]
SMARTiSM00165. UBA. 1 hit.
SM00166. UBX. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50030. UBA. 1 hit.
PS50033. UBX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBXN1_MOUSE
AccessioniPrimary (citable) accession number: Q922Y1
Secondary accession number(s): Q3UCP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.