Dual specificity tyrosine-phosphorylation-regulated kinase 3

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Q922Y0 (DYRK3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 76. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Dual specificity tyrosine-phosphorylation-regulated kinase 3
EC=2.7.12.1

Gene names

Name:

Dyrk3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	586 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Negative regulator of EPO-dependent erythropoiesis, may place an upper limit on red cell production during stress erythropoiesis. Inhibits cell death due to cytokine withdrawal in hematopoietic progenitor cells. May act by regulating CREB/CRE signaling. Ref.3 UniProtKB O43781
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. Ref.3
Cofactor	Magnesium By similarity. Ref.3
Subcellular location	Nucleus By similarity UniProtKB O43781.
Post-translational modification	Autophosphorylated on tyrosine residues By similarity. Ref.5 UniProtKB O43781
Disruption phenotype	Mice exhibit unperturbed steady-state erythropoiesis but significantly increased reticulocyte production during stress erythropoiesis and appear to be partially protected against anemia. Ref.4
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	erythrocyte differentiation Inferred from direct assay Ref.4. Source: UniProtKB
Cellular component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from direct assay Ref.3. Source: UniProtKB magnesium ion binding Inferred from direct assay Ref.3. Source: UniProtKB protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine/tyrosine kinase activity Inferred from electronic annotation. Source: EC protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Sirt1	Q923E4	7	EBI-5242007,EBI-1802585

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 586

586

Dual specificity tyrosine-phosphorylation-regulated kinase 3

PRO_0000291537

Regions

Domain

208 – 521

314

Protein kinase

Nucleotide binding

214 – 222

ATP By similarity UniProtKB P28523

Sites

Active site

334

Proton acceptor By similarity UniProtKB P28523

Binding site

237

ATP Ref.3

Amino acid modifications

Modified residue

208

Phosphotyrosine By similarity UniProtKB O43781

Modified residue

317

Phosphoserine Ref.5

Modified residue

368

Phosphotyrosine Ref.3

Experimental info

Mutagenesis

237

K → A: Loss of kinase activity. Ref.3

Mutagenesis

366

Y → A: Minimal loss of kinase activity. Ref.3

Mutagenesis

366

Y → E: Minimal loss of kinase activity; when associated with E-368. Ref.3

Mutagenesis

368

Y → A: Loss of kinase activity. Ref.3

Mutagenesis

368

Y → E: Minimal loss of kinase activity; when associated with E-366. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

Q922Y0 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 0097F7584726AB55
Show »

FASTA

586

65,572

        10         20         30         40         50         60 
MGGAARDRGR KDAALPGAGL PPQQRRLGDG VYDTFMMIDE TKGPPYSDTF SNPSEAPVSR 

        70         80         90        100        110        120 
RLNITTEPLT RGHTQHFVNG SEMKVEQLFQ EFGNRRSNTL QSDGISNSEK SSPASQGKSS 

       130        140        150        160        170        180 
ESLSAVKCNL SSRPSKVLPL TPEQALKQYK HHLTAYEKLE IVSYPEIYFV GPNAKKRQGV 

       190        200        210        220        230        240 
IGGPNNGGYD DADGAYIHVP RDHLAYRYEV LKIIGKGSFG QVARVYDHKL RQYVALKMVR 

       250        260        270        280        290        300 
NEKRFHRQAA EEIRILEHLK KQDKTGSMNV IHMLESFTFR NHVCMAFELL SIDLYELIKK 

       310        320        330        340        350        360 
NKFQGFSVQL VRKFAQSILQ SLDALHKNKI IHCDLKPENI LLKHHGRSAT KVIDFGSSCF 

       370        380        390        400        410        420 
EYQKLYTYIQ SRFYRAPEII LGCRYSTPID IWSFGCILAE LLTGQPLFPG EDEGDQLACM 

       430        440        450        460        470        480 
IELLGMPPQK LLEQSKRAKY FINSKGLPRY CSVSTQTDGR VVLLGGRSRR GKKRGPPGSK 

       490        500        510        520        530        540 
DWATALKGCG DYLFIEFLKR CLQWDPSARL TPAQALRHPW ISKSTPKPLT MDKVPGKRVV 

       550        560        570        580 
NPTNAFQGLG SKLPPVVGIA SKLKANLMSE TSGSIPLCSV LPKLIS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 49-586. Strain: C57BL/6J. Tissue: Embryo.
[3]	"DYRK3 activation, engagement of protein kinase A/cAMP response element-binding protein, and modulation of progenitor cell survival." Li K., Zhao S., Karur V., Wojchowski D.M. J. Biol. Chem. 277:47052-47060(2002) [PubMed: 12356771] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF LYS-237; TYR-366 AND TYR-368.
[4]	"Erythropoietin-dependent erythropoiesis: new insights and questions." Wojchowski D.M., Menon M.P., Sathyanarayana P., Fang J., Karur V., Houde E., Kapelle W., Bogachev O. Blood Cells Mol. Dis. 36:232-238(2006) [PubMed: 16524748] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[5]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-317, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC006704 mRNA. Translation: AAH06704.1. AK035114 mRNA. Translation: BAC28949.1.
IPI	IPI00123588.
RefSeq	NP_663483.1. NM_145508.2.
UniGene	Mm.39299.
3D structure databases
HSSP	HSSP built from PDB template 2BAJ based on UniProtKB Q16539.
ProteinModelPortal	Q922Y0.
SMR	Q922Y0. Positions 136-521.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q922Y0. 2 interactions.
STRING	Q922Y0.
PTM databases
PhosphoSite	Q922Y0.
Proteomic databases
PRIDE	Q922Y0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000016670; ENSMUSP00000016670; ENSMUSG00000016526.
GeneID	226419.
KEGG	mmu:226419.
UCSC	uc007cmw.1. mouse.
Organism-specific databases
CTD	8444.
MGI	MGI:1330300. Dyrk3.
Phylogenomic databases
eggNOG	roNOG05902.
GeneTree	ENSGT00570000079111.
HOGENOM	HBG755340.
HOVERGEN	HBG051426.
InParanoid	Q922Y0.
OMA	SEKCSPT.
OrthoDB	EOG48GW2W.
PhylomeDB	Q922Y0.
Gene expression databases
ArrayExpress	Q922Y0.
Bgee	Q922Y0.
Genevestigator	Q922Y0.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K08825.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	378148.
SOURCE	Search...

Entry information

Entry name

DYRK3_MOUSE

Accession

Primary (citable) accession number: Q922Y0
Secondary accession number(s): Q8BM34

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents