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Protein

Protein arginine N-methyltransferase 7

Gene

Prmt7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo (By similarity).By similarity1 Publication

Catalytic activityi

S-adenosyl-L-methionine + [protein]-L-arginine = S-adenosyl-L-homocysteine + [protein]-N(omega)-methyl-L-arginine.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei144 – 1441By similarity
Active sitei153 – 1531By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Transferase

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.125. 3474.
ReactomeiR-MMU-3214858. RMTs methylate histone arginines.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein arginine N-methyltransferase 7 (EC:2.1.1.321By similarity)
Alternative name(s):
Histone-arginine N-methyltransferase PRMT7
[Myelin basic protein]-arginine N-methyltransferase PRMT7
Gene namesi
Name:Prmt7
Synonyms:Kiaa1933
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2384879. Prmt7.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutants, shortly after birth, display significant reduced body size, reduced weight and shortened fifth metatarsals. They are subviable with about 45% of the expected number of expected mutant pups ar P14. Surviving adult mice exhibit increased fat mass, reduced length and limb anormalities. They also have reduced bone mineral content and density. Knockout mice show sexually dimorphic phenotypes, including changes in bone mineral content, bone mineral density and fifth metacarpal length that are only significant in females.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 692692Protein arginine N-methyltransferase 7PRO_0000212336Add
BLAST

Proteomic databases

EPDiQ922X9.
MaxQBiQ922X9.
PaxDbiQ922X9.
PeptideAtlasiQ922X9.
PRIDEiQ922X9.

PTM databases

iPTMnetiQ922X9.
PhosphoSiteiQ922X9.

Expressioni

Developmental stagei

Present in undifferentiated embryonic stem and germ cells; expression is lost when cells differentiate. In the developing testis, it is expressed at all stages. Present in all cells within the developing tubule, including gonocytes and spermatogonia (at protein level). It the developing kidney, it is confined to the nephrogenic zone in the cortical region, where the tips of the ureteric bud induce de novo formation of epithelia in the metanephric mesenchyme and early glomeruli. Expression is around 8-fold lower in adult kidneys.3 Publications

Gene expression databases

BgeeiENSMUSG00000060098.
CleanExiMM_PRMT7.
ExpressionAtlasiQ922X9. baseline and differential.
GenevisibleiQ922X9. MM.

Interactioni

Subunit structurei

Homodimer and heterodimer. Interacts with PRMT5 and SNRPD3 (By similarity). Interacts with CTCFL.By similarity1 Publication

Protein-protein interaction databases

BioGridi229540. 1 interaction.
DIPiDIP-29210N.
STRINGi10090.ENSMUSP00000071521.

Structurei

Secondary structure

1
692
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi28 – 314Combined sources
Helixi35 – 395Combined sources
Helixi42 – 6120Combined sources
Beta strandi67 – 726Combined sources
Helixi77 – 859Combined sources
Beta strandi88 – 936Combined sources
Helixi97 – 10913Combined sources
Turni113 – 1153Combined sources
Beta strandi116 – 1194Combined sources
Helixi123 – 1253Combined sources
Beta strandi132 – 1365Combined sources
Beta strandi138 – 1436Combined sources
Helixi155 – 16511Combined sources
Beta strandi167 – 1759Combined sources
Beta strandi177 – 18610Combined sources
Helixi188 – 1914Combined sources
Turni192 – 1943Combined sources
Beta strandi199 – 2035Combined sources
Beta strandi206 – 2105Combined sources
Helixi214 – 2185Combined sources
Beta strandi226 – 2283Combined sources
Helixi230 – 2323Combined sources
Helixi235 – 2373Combined sources
Beta strandi245 – 2517Combined sources
Beta strandi261 – 2677Combined sources
Beta strandi273 – 28715Combined sources
Beta strandi292 – 2954Combined sources
Helixi299 – 3013Combined sources
Helixi305 – 3073Combined sources
Beta strandi312 – 3143Combined sources
Beta strandi316 – 32712Combined sources
Beta strandi333 – 3397Combined sources
Beta strandi344 – 3485Combined sources
Helixi370 – 3734Combined sources
Helixi376 – 3827Combined sources
Helixi385 – 39814Combined sources
Beta strandi404 – 4096Combined sources
Helixi414 – 4207Combined sources
Beta strandi424 – 4296Combined sources
Helixi433 – 44513Combined sources
Turni449 – 4513Combined sources
Beta strandi452 – 4554Combined sources
Helixi459 – 4613Combined sources
Helixi465 – 4673Combined sources
Beta strandi472 – 4765Combined sources
Helixi487 – 4915Combined sources
Helixi492 – 4998Combined sources
Helixi501 – 5033Combined sources
Beta strandi504 – 5129Combined sources
Beta strandi514 – 52613Combined sources
Helixi527 – 5315Combined sources
Beta strandi535 – 5373Combined sources
Helixi543 – 55311Combined sources
Beta strandi555 – 5584Combined sources
Beta strandi562 – 5643Combined sources
Helixi566 – 5683Combined sources
Beta strandi571 – 5755Combined sources
Beta strandi578 – 5847Combined sources
Beta strandi594 – 6029Combined sources
Beta strandi610 – 62011Combined sources
Beta strandi623 – 6308Combined sources
Beta strandi647 – 6515Combined sources
Beta strandi667 – 6748Combined sources
Turni676 – 6783Combined sources
Beta strandi681 – 6888Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4C4AX-ray1.70A1-692[»]
ProteinModelPortaliQ922X9.
SMRiQ922X9. Positions 27-688.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 345332SAM-dependent MTase PRMT-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini358 – 684327SAM-dependent MTase PRMT-type 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT7 subfamily.PROSITE-ProRule annotation
Contains 2 SAM-dependent MTase PRMT-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410INRX. Eukaryota.
COG0500. LUCA.
GeneTreeiENSGT00530000063495.
HOGENOMiHOG000033951.
HOVERGENiHBG018729.
InParanoidiQ922X9.
KOiK11438.
OMAiGENSWQE.
OrthoDBiEOG091G07KL.
PhylomeDBiQ922X9.
TreeFamiTF315221.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR025799. Arg_MeTrfase.
IPR014644. MeTrfase_PRMT7.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11006. PTHR11006. 1 hit.
PIRSFiPIRSF036946. Arg_N-mtase. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51678. SAM_MT_PRMT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q922X9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVFCGRANP TTGSLEWLEE DEHYDYHQEI ARSSYADMLH DKDRNIKYYQ
60 70 80 90 100
GIRAAVSRVK DRGQKALVLD IGTGTGLLSM MAVTAGADFC YAIEVFKPMA
110 120 130 140 150
EAAVKIVERN GFSDKIKVIN KHSTEVTVGP DGDLPCRANI LITELFDTEL
160 170 180 190 200
IGEGALPSYE HAHKHLVQED CEAVPHRATV YAQLVESRRM WSWNKLFPVR
210 220 230 240 250
VRTSLGEQVI VPPSELERCP GAPSVCDIQL NQVSPADFTV LSDVLPMFSV
260 270 280 290 300
DFSKQVSSSA ACHSRQFVPL ASGQAQVVLS WWDIEMDPEG KIKCTMAPFW
310 320 330 340 350
AQTDPQELQW RDHWMQCVYF LPQEEPVVQG SPRCLVAHHD DYCVWYSLQR
360 370 380 390 400
TSPDENDSAY QVRPVCDCQA HLLWNRPRFG EINDQDRTDH YAQALRTVLL
410 420 430 440 450
PGSVCLCVSD GSLLSMLAHH LGAEQVFTVE SSVASYRLMK RIFKVNHLED
460 470 480 490 500
KISVINKRPE LLTAADLEGK KVSLLLGEPF FTTSLLPWHN LYFWYVRTSV
510 520 530 540 550
DQHLAPGAVV MPQAASLHAV IVEFRDLWRI RSPCGDCEGF DVHIMDDMIK
560 570 580 590 600
HSLDFRESRE AEPHPLWEYP CRSLSKPQEI LTFDFQQPIP QQPMQSKGTM
610 620 630 640 650
ELTRPGKSHG AVLWMEYQLT PDSTISTGLI NPAEDKGDCC WNPHCKQAVY
660 670 680 690
FLSTTLDLRV PLNGPRSVSY VVEFHPLTGD ITMEFRLADT LS
Length:692
Mass (Da):78,301
Last modified:December 1, 2001 - v1
Checksum:iD61A9500956BF02D
GO

Sequence cautioni

The sequence BAD32582 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371D → E in AAH57177 (PubMed:15489334).Curated
Sequence conflicti612 – 6121V → G in BAE36880 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY673972 mRNA. Translation: AAT76979.1.
AK173304 mRNA. Translation: BAD32582.1. Different initiation.
AK154255 mRNA. Translation: BAE32467.1.
AK162376 mRNA. Translation: BAE36880.1.
CH466525 Genomic DNA. Translation: EDL11358.1.
BC006705 mRNA. Translation: AAH06705.1.
BC057177 mRNA. Translation: AAH57177.1.
CCDSiCCDS22633.1.
RefSeqiNP_663379.1. NM_145404.1.
UniGeneiMm.251804.
Mm.446217.

Genome annotation databases

EnsembliENSMUST00000071592; ENSMUSP00000071521; ENSMUSG00000060098.
GeneIDi214572.
KEGGimmu:214572.
UCSCiuc009nfu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY673972 mRNA. Translation: AAT76979.1.
AK173304 mRNA. Translation: BAD32582.1. Different initiation.
AK154255 mRNA. Translation: BAE32467.1.
AK162376 mRNA. Translation: BAE36880.1.
CH466525 Genomic DNA. Translation: EDL11358.1.
BC006705 mRNA. Translation: AAH06705.1.
BC057177 mRNA. Translation: AAH57177.1.
CCDSiCCDS22633.1.
RefSeqiNP_663379.1. NM_145404.1.
UniGeneiMm.251804.
Mm.446217.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4C4AX-ray1.70A1-692[»]
ProteinModelPortaliQ922X9.
SMRiQ922X9. Positions 27-688.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229540. 1 interaction.
DIPiDIP-29210N.
STRINGi10090.ENSMUSP00000071521.

PTM databases

iPTMnetiQ922X9.
PhosphoSiteiQ922X9.

Proteomic databases

EPDiQ922X9.
MaxQBiQ922X9.
PaxDbiQ922X9.
PeptideAtlasiQ922X9.
PRIDEiQ922X9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000071592; ENSMUSP00000071521; ENSMUSG00000060098.
GeneIDi214572.
KEGGimmu:214572.
UCSCiuc009nfu.1. mouse.

Organism-specific databases

CTDi54496.
MGIiMGI:2384879. Prmt7.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410INRX. Eukaryota.
COG0500. LUCA.
GeneTreeiENSGT00530000063495.
HOGENOMiHOG000033951.
HOVERGENiHBG018729.
InParanoidiQ922X9.
KOiK11438.
OMAiGENSWQE.
OrthoDBiEOG091G07KL.
PhylomeDBiQ922X9.
TreeFamiTF315221.

Enzyme and pathway databases

BRENDAi2.1.1.125. 3474.
ReactomeiR-MMU-3214858. RMTs methylate histone arginines.

Miscellaneous databases

ChiTaRSiPrmt7. mouse.
PROiQ922X9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000060098.
CleanExiMM_PRMT7.
ExpressionAtlasiQ922X9. baseline and differential.
GenevisibleiQ922X9. MM.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR025799. Arg_MeTrfase.
IPR014644. MeTrfase_PRMT7.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11006. PTHR11006. 1 hit.
PIRSFiPIRSF036946. Arg_N-mtase. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51678. SAM_MT_PRMT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANM7_MOUSE
AccessioniPrimary (citable) accession number: Q922X9
Secondary accession number(s): Q3TRZ6
, Q69Z62, Q6B955, Q6PG80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: December 1, 2001
Last modified: September 7, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.