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Protein

Pyrroline-5-carboxylate reductase 1, mitochondrial

Gene

Pycr1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress (By similarity).By similarity

Catalytic activityi

L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H.

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-proline from L-glutamate 5-semialdehyde.
Proteins known to be involved in this subpathway in this organism are:
  1. Pyrroline-5-carboxylate reductase 3 (Pycrl), Pyrroline-5-carboxylate reductase 1, mitochondrial (Pycr1), Pyrroline-5-carboxylate reductase 2 (Pycr2), Pyrroline-5-carboxylate reductase (Pycr2), Pyrroline-5-carboxylate reductase (Pycr1), Pyrroline-5-carboxylate reductase (Pycr2)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-proline from L-glutamate 5-semialdehyde, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34NADP; via carbonyl oxygenBy similarity1
Binding sitei56NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi6 – 11NADPBy similarity6
Nucleotide bindingi69 – 72NADPBy similarity4
Nucleotide bindingi95 – 97NADPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis, Stress response

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiR-MMU-70614. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00098; UER00361.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrroline-5-carboxylate reductase 1, mitochondrial (EC:1.5.1.2)
Short name:
P5C reductase 1
Short name:
P5CR 1
Gene namesi
Name:Pycr1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2384795. Pycr1.

Subcellular locationi

  • Mitochondrion By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001873152 – 309Pyrroline-5-carboxylate reductase 1, mitochondrialAdd BLAST308

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei278PhosphoserineBy similarity1
Modified residuei301PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ922W5.
MaxQBiQ922W5.
PaxDbiQ922W5.
PRIDEiQ922W5.

PTM databases

iPTMnetiQ922W5.
PhosphoSitePlusiQ922W5.

Expressioni

Tissue specificityi

Highly expressed in osteoblasts and skin.1 Publication

Gene expression databases

BgeeiENSMUSG00000025140.
CleanExiMM_PYCR1.
ExpressionAtlasiQ922W5. baseline and differential.
GenevisibleiQ922W5. MM.

Interactioni

Subunit structurei

Homodecamer; composed of 5 homodimers.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ922W5. 1 interactor.
STRINGi10090.ENSMUSP00000026133.

Structurei

3D structure databases

ProteinModelPortaliQ922W5.
SMRiQ922W5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3124. Eukaryota.
COG0345. LUCA.
GeneTreeiENSGT00390000007443.
HOGENOMiHOG000230247.
HOVERGENiHBG053399.
InParanoidiQ922W5.
KOiK00286.
OMAiRTFNEHQ.
OrthoDBiEOG091G0KMI.
PhylomeDBiQ922W5.

Family and domain databases

Gene3Di1.10.3730.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01925. P5C_reductase. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR029036. P5CR_dimer.
IPR028939. ProC_N.
IPR000304. Pyrroline-COOH_reductase.
[Graphical view]
PANTHERiPTHR11645. PTHR11645. 1 hit.
PfamiPF03807. F420_oxidored. 1 hit.
PF14748. P5CR_dimer. 1 hit.
[Graphical view]
PIRSFiPIRSF000193. Pyrrol-5-carb_rd. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00112. proC. 1 hit.
PROSITEiPS00521. P5CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q922W5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVGFIGAGQ LAFALAKGFT AAGVLAAHKI MASSPDMDQA TVSALRKIGV
60 70 80 90 100
NLTPHNKETV RHSDVLFLAV KPHIIPFILD EIGANIEDRH IVVSCAAGVT
110 120 130 140 150
INSIEKKLTA FQPAPKVIRC MTNTPVVVRE GVTVYATGTH AQVEDGRLVE
160 170 180 190 200
QLMGSVGFCT EVEEDLIDAV TGLSGSGPAY AFTALDALAD GGVKMGLPRR
210 220 230 240 250
LAVRLGAQAL LGAAKMLLDS EQHPSQLKDN VCSPGGATIH ALHVLESGGF
260 270 280 290 300
RSLLINAVEA SCIRTRELQT MADQETISPA AIKKTVLDKV KLDSSAGASL

SSDHVKPLP
Length:309
Mass (Da):32,373
Last modified:December 1, 2001 - v1
Checksum:iA3CD24AACDD53DEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC006727 mRNA. Translation: AAH06727.1.
CCDSiCCDS25749.1.
RefSeqiNP_659044.1. NM_144795.3.
XP_006532798.1. XM_006532735.2.
XP_011247157.1. XM_011248855.2.
UniGeneiMm.127731.

Genome annotation databases

EnsembliENSMUST00000026133; ENSMUSP00000026133; ENSMUSG00000025140.
ENSMUST00000170556; ENSMUSP00000131199; ENSMUSG00000025140.
GeneIDi209027.
KEGGimmu:209027.
UCSCiuc007mtv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC006727 mRNA. Translation: AAH06727.1.
CCDSiCCDS25749.1.
RefSeqiNP_659044.1. NM_144795.3.
XP_006532798.1. XM_006532735.2.
XP_011247157.1. XM_011248855.2.
UniGeneiMm.127731.

3D structure databases

ProteinModelPortaliQ922W5.
SMRiQ922W5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ922W5. 1 interactor.
STRINGi10090.ENSMUSP00000026133.

PTM databases

iPTMnetiQ922W5.
PhosphoSitePlusiQ922W5.

Proteomic databases

EPDiQ922W5.
MaxQBiQ922W5.
PaxDbiQ922W5.
PRIDEiQ922W5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026133; ENSMUSP00000026133; ENSMUSG00000025140.
ENSMUST00000170556; ENSMUSP00000131199; ENSMUSG00000025140.
GeneIDi209027.
KEGGimmu:209027.
UCSCiuc007mtv.1. mouse.

Organism-specific databases

CTDi5831.
MGIiMGI:2384795. Pycr1.

Phylogenomic databases

eggNOGiKOG3124. Eukaryota.
COG0345. LUCA.
GeneTreeiENSGT00390000007443.
HOGENOMiHOG000230247.
HOVERGENiHBG053399.
InParanoidiQ922W5.
KOiK00286.
OMAiRTFNEHQ.
OrthoDBiEOG091G0KMI.
PhylomeDBiQ922W5.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00361.
ReactomeiR-MMU-70614. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

PROiQ922W5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025140.
CleanExiMM_PYCR1.
ExpressionAtlasiQ922W5. baseline and differential.
GenevisibleiQ922W5. MM.

Family and domain databases

Gene3Di1.10.3730.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01925. P5C_reductase. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR029036. P5CR_dimer.
IPR028939. ProC_N.
IPR000304. Pyrroline-COOH_reductase.
[Graphical view]
PANTHERiPTHR11645. PTHR11645. 1 hit.
PfamiPF03807. F420_oxidored. 1 hit.
PF14748. P5CR_dimer. 1 hit.
[Graphical view]
PIRSFiPIRSF000193. Pyrrol-5-carb_rd. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00112. proC. 1 hit.
PROSITEiPS00521. P5CR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP5CR1_MOUSE
AccessioniPrimary (citable) accession number: Q922W5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.