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Protein

Pyrroline-5-carboxylate reductase 1, mitochondrial

Gene

Pycr1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress (By similarity).By similarity

Catalytic activityi

L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341NADP; via carbonyl oxygenBy similarity
Binding sitei56 – 561NADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi6 – 116NADPBy similarity
Nucleotide bindingi69 – 724NADPBy similarity
Nucleotide bindingi95 – 973NADPBy similarity

GO - Molecular functioni

  1. identical protein binding Source: MGI
  2. pyrroline-5-carboxylate reductase activity Source: UniProtKB

GO - Biological processi

  1. cellular response to oxidative stress Source: UniProtKB
  2. L-proline biosynthetic process Source: UniProtKB-UniPathway
  3. negative regulation of hydrogen peroxide-induced cell death Source: MGI
  4. proline biosynthetic process Source: UniProtKB
  5. regulation of mitochondrial membrane potential Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis, Stress response

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiREACT_239550. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00098; UER00361.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrroline-5-carboxylate reductase 1, mitochondrial (EC:1.5.1.2)
Short name:
P5C reductase 1
Short name:
P5CR 1
Gene namesi
Name:Pycr1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:2384795. Pycr1.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 309308Pyrroline-5-carboxylate reductase 1, mitochondrialPRO_0000187315Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ922W5.
PaxDbiQ922W5.
PRIDEiQ922W5.

PTM databases

PhosphoSiteiQ922W5.

Expressioni

Tissue specificityi

Highly expressed in osteoblasts and skin.1 Publication

Gene expression databases

BgeeiQ922W5.
CleanExiMM_PYCR1.
ExpressionAtlasiQ922W5. baseline and differential.
GenevestigatoriQ922W5.

Interactioni

Subunit structurei

Homodecamer; composed of 5 homodimers.By similarity

Protein-protein interaction databases

IntActiQ922W5. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ922W5.
SMRiQ922W5. Positions 1-275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0345.
GeneTreeiENSGT00390000007443.
HOGENOMiHOG000230247.
HOVERGENiHBG053399.
InParanoidiQ922W5.
KOiK00286.
OMAiRTFNEHQ.
OrthoDBiEOG7N0C5N.
PhylomeDBiQ922W5.

Family and domain databases

Gene3Di1.10.3730.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01925. P5C_reductase.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR029036. P5CR_dimer.
IPR028939. ProC_N.
IPR000304. Pyrroline-COOH_reductase.
[Graphical view]
PANTHERiPTHR11645. PTHR11645. 1 hit.
PfamiPF03807. F420_oxidored. 1 hit.
PF14748. P5CR_dimer. 1 hit.
[Graphical view]
PIRSFiPIRSF000193. Pyrrol-5-carb_rd. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR00112. proC. 1 hit.
PROSITEiPS00521. P5CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q922W5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVGFIGAGQ LAFALAKGFT AAGVLAAHKI MASSPDMDQA TVSALRKIGV
60 70 80 90 100
NLTPHNKETV RHSDVLFLAV KPHIIPFILD EIGANIEDRH IVVSCAAGVT
110 120 130 140 150
INSIEKKLTA FQPAPKVIRC MTNTPVVVRE GVTVYATGTH AQVEDGRLVE
160 170 180 190 200
QLMGSVGFCT EVEEDLIDAV TGLSGSGPAY AFTALDALAD GGVKMGLPRR
210 220 230 240 250
LAVRLGAQAL LGAAKMLLDS EQHPSQLKDN VCSPGGATIH ALHVLESGGF
260 270 280 290 300
RSLLINAVEA SCIRTRELQT MADQETISPA AIKKTVLDKV KLDSSAGASL

SSDHVKPLP
Length:309
Mass (Da):32,373
Last modified:December 1, 2001 - v1
Checksum:iA3CD24AACDD53DEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC006727 mRNA. Translation: AAH06727.1.
CCDSiCCDS25749.1.
RefSeqiNP_659044.1. NM_144795.3.
XP_006532798.1. XM_006532735.1.
UniGeneiMm.127731.

Genome annotation databases

EnsembliENSMUST00000026133; ENSMUSP00000026133; ENSMUSG00000025140.
ENSMUST00000170556; ENSMUSP00000131199; ENSMUSG00000025140.
GeneIDi209027.
KEGGimmu:209027.
UCSCiuc007mtv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC006727 mRNA. Translation: AAH06727.1.
CCDSiCCDS25749.1.
RefSeqiNP_659044.1. NM_144795.3.
XP_006532798.1. XM_006532735.1.
UniGeneiMm.127731.

3D structure databases

ProteinModelPortaliQ922W5.
SMRiQ922W5. Positions 1-275.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ922W5. 1 interaction.

PTM databases

PhosphoSiteiQ922W5.

Proteomic databases

MaxQBiQ922W5.
PaxDbiQ922W5.
PRIDEiQ922W5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026133; ENSMUSP00000026133; ENSMUSG00000025140.
ENSMUST00000170556; ENSMUSP00000131199; ENSMUSG00000025140.
GeneIDi209027.
KEGGimmu:209027.
UCSCiuc007mtv.1. mouse.

Organism-specific databases

CTDi5831.
MGIiMGI:2384795. Pycr1.

Phylogenomic databases

eggNOGiCOG0345.
GeneTreeiENSGT00390000007443.
HOGENOMiHOG000230247.
HOVERGENiHBG053399.
InParanoidiQ922W5.
KOiK00286.
OMAiRTFNEHQ.
OrthoDBiEOG7N0C5N.
PhylomeDBiQ922W5.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00361.
ReactomeiREACT_239550. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

NextBioi372532.
PROiQ922W5.
SOURCEiSearch...

Gene expression databases

BgeeiQ922W5.
CleanExiMM_PYCR1.
ExpressionAtlasiQ922W5. baseline and differential.
GenevestigatoriQ922W5.

Family and domain databases

Gene3Di1.10.3730.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01925. P5C_reductase.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR029036. P5CR_dimer.
IPR028939. ProC_N.
IPR000304. Pyrroline-COOH_reductase.
[Graphical view]
PANTHERiPTHR11645. PTHR11645. 1 hit.
PfamiPF03807. F420_oxidored. 1 hit.
PF14748. P5CR_dimer. 1 hit.
[Graphical view]
PIRSFiPIRSF000193. Pyrrol-5-carb_rd. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR00112. proC. 1 hit.
PROSITEiPS00521. P5CR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  2. Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiP5CR1_MOUSE
AccessioniPrimary (citable) accession number: Q922W5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: December 1, 2001
Last modified: March 4, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.