Q922W5 (P5CR1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyrroline-5-carboxylate reductase 1, mitochondrial
Short name=P5C reductase 1
Short name=P5CR 1
EC=1.5.1.2

Gene names

Name:

Pycr1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress By similarity.
Catalytic activity	L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.
Subunit structure	Homodecamer; composed of 5 homodimers By similarity.
Subcellular location	Mitochondrion Ref.2.
Tissue specificity	Highly expressed in osteoblasts and skin. Ref.3
Sequence similarities	Belongs to the pyrroline-5-carboxylate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis Stress response
Cellular component	Mitochondrion
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to oxidative stress Inferred from sequence or structural similarity. Source: UniProtKB proline biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro pyrroline-5-carboxylate reductase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 309	308	Pyrroline-5-carboxylate reductase 1, mitochondrial		PRO_0000187315
Regions
	Nucleotide binding	6 – 11	6	NADP By similarity
	Nucleotide binding	69 – 72	4	NADP By similarity
	Nucleotide binding	95 – 97	3	NADP By similarity
Sites
	Binding site	34	1	NADP; via carbonyl oxygen By similarity
	Binding site	56	1	NADP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q922W5 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: A3CD24AACDD53DEF	FASTA	309	32,373
	10 20 30 40 50 60 MSVGFIGAGQ LAFALAKGFT AAGVLAAHKI MASSPDMDQA TVSALRKIGV NLTPHNKETV 70 80 90 100 110 120 RHSDVLFLAV KPHIIPFILD EIGANIEDRH IVVSCAAGVT INSIEKKLTA FQPAPKVIRC 130 140 150 160 170 180 MTNTPVVVRE GVTVYATGTH AQVEDGRLVE QLMGSVGFCT EVEEDLIDAV TGLSGSGPAY 190 200 210 220 230 240 AFTALDALAD GGVKMGLPRR LAVRLGAQAL LGAAKMLLDS EQHPSQLKDN VCSPGGATIH 250 260 270 280 290 300 ALHVLESGGF RSLLINAVEA SCIRTRELQT MADQETISPA AIKKTVLDKV KLDSSAGASL SSDHVKPLP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[2]	"Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria." Mootha V.K., Bunkenborg J., Olsen J.V., Hjerrild M., Wisniewski J.R., Stahl E., Bolouri M.S., Ray H.N., Sihag S., Kamal M., Patterson N., Lander E.S., Mann M. Cell 115:629-640(2003) [PubMed: 14651853] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION. Strain: C57BL/6J.
[3]	"Mutations in PYCR1 cause cutis laxa with progeroid features." Reversade B., Escande-Beillard N., Dimopoulou A., Fischer B., Chng S.C., Li Y., Shboul M., Tham P.-Y., Kayserili H., Al-Gazali L., Shahwan M., Brancati F., Lee H., O'Connor B.D., Schmidt-von Kegler M., Merriman B., Nelson S.F., Masri A. , Alkazaleh F., Guerra D., Ferrari P., Nanda A., Rajab A., Markie D., Gray M., Nelson J., Grix A., Sommer A., Savarirayan R., Janecke A.R., Steichen E., Sillence D., Hausser I., Budde B., Nuernberg G., Nuernberg P., Seemann P., Kunkel D., Zambruno G., Dallapiccola B., Schuelke M., Robertson S., Hamamy H., Wollnik B., Van Maldergem L., Mundlos S., Kornak U. Nat. Genet. 41:1016-1021(2009) [PubMed: 19648921] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC006727 mRNA. Translation: AAH06727.1.
IPI	IPI00123510.
RefSeq	NP_659044.1. NM_144795.3.
UniGene	Mm.127731.
3D structure databases
ProteinModelPortal	Q922W5.
SMR	Q922W5. Positions 1-275.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q922W5. 1 interaction.
STRING	Q922W5.
PTM databases
PhosphoSite	Q922W5.
Proteomic databases
PRIDE	Q922W5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000026133; ENSMUSP00000026133; ENSMUSG00000025140. ENSMUST00000170556; ENSMUSP00000131199; ENSMUSG00000025140.
GeneID	209027.
KEGG	mmu:209027.
NMPDR	fig|10090.3.peg.25811.
Organism-specific databases
CTD	5831.
MGI	MGI:2384795. Pycr1.
Phylogenomic databases
GeneTree	ENSGT00390000007443.
HOGENOM	HBG726602.
HOVERGEN	HBG053399.
InParanoid	Q922W5.
OMA	SSGLVAW.
OrthoDB	EOG4PZJ76.
PhylomeDB	Q922W5.
Gene expression databases
ArrayExpress	Q922W5.
Bgee	Q922W5.
CleanEx	MM_PYCR1.
Genevestigator	Q922W5.
GermOnline	ENSMUSG00000025140. Mus musculus.
Family and domain databases
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR016040. NAD(P)-bd_dom. IPR004455. NADP_OxRdtase_F420. IPR000304. Pyrroline-COOH_reductase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00286.
PANTHER	PTHR11645. P5CR. 1 hit.
Pfam	PF03807. F420_oxidored. 1 hit. [Graphical view]
PIRSF	PIRSF000193. Pyrrol-5-carb_rd. 1 hit.
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00112. ProC. 1 hit.
PROSITE	PS00521. P5CR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	372532.
SOURCE	Search...

Entry information

Entry name

P5CR1_MOUSE

Accession

Primary (citable) accession number: Q922W5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	December 1, 2001
Last modified:	December 14, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program