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Protein

U4/U6 small nuclear ribonucleoprotein Prp3

Gene

Prpf3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
U4/U6 small nuclear ribonucleoprotein Prp3
Alternative name(s):
Pre-mRNA-splicing factor 3
Gene namesi
Name:Prpf3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1918017 Prpf3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000970451 – 683U4/U6 small nuclear ribonucleoprotein Prp3Add BLAST683

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki139Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei164PhosphoserineBy similarity1
Cross-linki244Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki252Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei619PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated. Undergoes 'Lys-63'-linked polyubiquitination by PRPF19 and deubiquitination by USP4. 'Lys-63'-linked ubiquitination increases the affinity for PRPF8 and may regulate the assembly of the U4/U6-U5 tri-snRNP complex.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ922U1
MaxQBiQ922U1
PaxDbiQ922U1
PeptideAtlasiQ922U1
PRIDEiQ922U1

PTM databases

iPTMnetiQ922U1
PhosphoSitePlusiQ922U1

Expressioni

Gene expression databases

BgeeiENSMUSG00000015748
CleanExiMM_PRPF3
ExpressionAtlasiQ922U1 baseline and differential
GenevisibleiQ922U1 MM

Interactioni

Subunit structurei

Interacts directly with PRPF4. Part of a heteromeric complex containing PPIH, PRPF3 and PRPF4 that is stable in the absence of RNA. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39. Interacts with SART3; the interaction is direct and recruits the deubiquitinase USP4 to PRPF3. Interacts with PRPF19. Interacts ('Lys-63'-linked polyubiquitinated) with PRPF8 (via the MPN (JAB/Mov34) domain); may stabilize the U4/U6-U5 tri-snRNP complex.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi214242, 21 interactors
IntActiQ922U1, 22 interactors
MINTiQ922U1
STRINGi10090.ENSMUSP00000015892

Structurei

3D structure databases

ProteinModelPortaliQ922U1
SMRiQ922U1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 87PWIPROSITE-ProRule annotationAdd BLAST87

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni416 – 550Mediates interaction with SART3By similarityAdd BLAST135

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi579 – 584Poly-Val6

Phylogenomic databases

eggNOGiKOG2769 Eukaryota
ENOG410XQV0 LUCA
GeneTreeiENSGT00390000011497
HOGENOMiHOG000205412
HOVERGENiHBG053699
InParanoidiQ922U1
KOiK12843
OMAiYRRLMLH
OrthoDBiEOG091G05VO
PhylomeDBiQ922U1
TreeFamiTF313082

Family and domain databases

InterProiView protein in InterPro
IPR010541 DUF1115
IPR013881 Pre-mRNA_splic_Prp3
IPR027104 Prp3
IPR002483 PWI_dom
IPR036483 PWI_dom_sf
PANTHERiPTHR14212 PTHR14212, 1 hit
PfamiView protein in Pfam
PF06544 DUF1115, 1 hit
PF08572 PRP3, 1 hit
PF01480 PWI, 1 hit
SMARTiView protein in SMART
SM00311 PWI, 1 hit
SUPFAMiSSF101233 SSF101233, 1 hit
PROSITEiView protein in PROSITE
PS51025 PWI, 1 hit

Sequencei

Sequence statusi: Complete.

Q922U1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSKRELDE LKPWIEKTVK RVLGFSEPTV VTAALNCVGK GMDKKKAADH
60 70 80 90 100
LKPFLDDSTL RFVDKLFEAV EEGRSSRHSK SSSDRSRKRE LKEVFGDDSE
110 120 130 140 150
ISKESSGVKK RRIPRFEEVE EEPEVIPGPP SESPGMLTKL QIKQMMEAAT
160 170 180 190 200
RQIEERKKQL SFISPPAPQP KTPSSSQPER LPIGNTIQPS QAATFMNDAI
210 220 230 240 250
EKARKAAELQ ARIQAQLALK PGLIGNANMV GLANLHAMGI APPKVELKDQ
260 270 280 290 300
TKPTPLILDE QGRTVDATGK EVELTHRMPT LKANIRAVKR EQFKQQLKEK
310 320 330 340 350
PSEDMESNTF FDPRVSIAPS QRQRRTFKFH DKGKFEKIAQ RLRTKAQLEK
360 370 380 390 400
LQAEISQAAR KTGIHTSTRL ALIAPKKELK EGDIPEIEWW DSYIIPNGFD
410 420 430 440 450
LTEENPKRED YFGITNLVEH PAQLNPPVDN DTPVTLGVYL TKKEQKKLRR
460 470 480 490 500
QTRREAQKEL QEKVRLGLTP PPEPKVRISN LMRVLGTEAV QDPTKVEAHV
510 520 530 540 550
RAQMAKRQKA HEEANAARKL TAEQRKVKKI KKLKEDISQG VHISVYRVRN
560 570 580 590 600
LSNPAKKFKI EANAGQLYLT GVVVLHKDVN VVVVEGGPKA QKKFKRLMLH
610 620 630 640 650
RIKWDEQTSN TKGDDDEESD EEAVKKTNKC VLVWEGTAKD RSFGEMKFKQ
660 670 680
CPTENMAREH FKKHGAEHYW DLALSESVLE STD
Length:683
Mass (Da):77,455
Last modified:December 1, 2001 - v1
Checksum:i988219CF88E0EC4A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78H → L in BAB29324 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014398 mRNA Translation: BAB29324.1
AK088194 mRNA Translation: BAC40202.1
AK167435 mRNA Translation: BAE39521.1
BC006782 mRNA Translation: AAH06782.1
CCDSiCCDS17621.1
RefSeqiNP_001303680.1, NM_001316751.1
NP_081817.2, NM_027541.4
UniGeneiMm.279872

Genome annotation databases

EnsembliENSMUST00000015892; ENSMUSP00000015892; ENSMUSG00000015748
ENSMUST00000161476; ENSMUSP00000124950; ENSMUSG00000015748
GeneIDi70767
KEGGimmu:70767
UCSCiuc008qlf.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPRPF3_MOUSE
AccessioniPrimary (citable) accession number: Q922U1
Secondary accession number(s): Q3TJH4, Q9D6C6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: December 1, 2001
Last modified: April 25, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health