Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

U4/U6 small nuclear ribonucleoprotein Prp3

Gene

Prpf3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.By similarity

GO - Molecular functioni

  1. identical protein binding Source: MGI
  2. poly(A) RNA binding Source: MGI

GO - Biological processi

  1. mRNA splicing, via spliceosome Source: GO_Central
  2. spliceosomal tri-snRNP complex assembly Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
U4/U6 small nuclear ribonucleoprotein Prp3
Alternative name(s):
Pre-mRNA-splicing factor 3
Gene namesi
Name:Prpf3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:1918017. Prpf3.

Subcellular locationi

Nucleus speckle PROSITE-ProRule annotation
Note: Colocalizes with spliceosomal snRNPs.By similarity

GO - Cellular componenti

  1. Cajal body Source: MGI
  2. cytoplasm Source: MGI
  3. nuclear speck Source: UniProtKB-SubCell
  4. nucleoplasm Source: MGI
  5. nucleus Source: UniProtKB
  6. spliceosomal complex Source: UniProtKB-KW
  7. U4/U6 x U5 tri-snRNP complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 683683U4/U6 small nuclear ribonucleoprotein Prp3PRO_0000097045Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei164 – 1641PhosphoserineBy similarity
Modified residuei619 – 6191PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated. Undergoes 'Lys-63'-linked polyubiquitination by PRPF19 and deubiquitination by USP4. 'Lys-63'-linked ubiquitination increases the affinity for PRPF8 and may regulate the assembly of the U4/U6-U5 tri-snRNP complex.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ922U1.
PaxDbiQ922U1.
PRIDEiQ922U1.

PTM databases

PhosphoSiteiQ922U1.

Expressioni

Gene expression databases

BgeeiQ922U1.
CleanExiMM_PRPF3.
ExpressionAtlasiQ922U1. baseline and differential.
GenevestigatoriQ922U1.

Interactioni

Subunit structurei

Interacts directly with PRPF4. Part of a heteromeric complex containing PPIH, PRPF3 and PRPF4 that is stable in the absence of RNA. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, NHP2L1, EFTUD2, SART1 and USP39. Interacts with SART3; the interaction is direct and recruits the deubiquitinase USP4 to PRPF3. Interacts with PRPF19. Interacts ('Lys-63'-linked polyubiquitinated) with PRPF8 (via the MPN (JAB/Mov34) domain); may stabilize the U4/U6-U5 tri-snRNP complex.By similarity

Protein-protein interaction databases

IntActiQ922U1. 2 interactions.
MINTiMINT-4108680.
STRINGi10090.ENSMUSP00000015892.

Structurei

3D structure databases

ProteinModelPortaliQ922U1.
SMRiQ922U1. Positions 1-73.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 8787PWIPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni416 – 550135Mediates interaction with SART3By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi579 – 5846Poly-Val

Sequence similaritiesi

Contains 1 PWI domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG268901.
GeneTreeiENSGT00390000011497.
HOGENOMiHOG000205412.
HOVERGENiHBG053699.
InParanoidiQ922U1.
KOiK12843.
OMAiPWVERTV.
OrthoDBiEOG789C9P.
PhylomeDBiQ922U1.
TreeFamiTF313082.

Family and domain databases

Gene3Di1.20.1390.10. 1 hit.
InterProiIPR010541. DUF1115.
IPR013881. Pre-mRNA_splic_Prp3.
IPR027104. Prp3.
IPR002483. PWI_dom.
[Graphical view]
PANTHERiPTHR14212. PTHR14212. 1 hit.
PfamiPF06544. DUF1115. 1 hit.
PF08572. PRP3. 1 hit.
PF01480. PWI. 1 hit.
[Graphical view]
SMARTiSM00311. PWI. 1 hit.
[Graphical view]
SUPFAMiSSF101233. SSF101233. 1 hit.
PROSITEiPS51025. PWI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q922U1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSKRELDE LKPWIEKTVK RVLGFSEPTV VTAALNCVGK GMDKKKAADH
60 70 80 90 100
LKPFLDDSTL RFVDKLFEAV EEGRSSRHSK SSSDRSRKRE LKEVFGDDSE
110 120 130 140 150
ISKESSGVKK RRIPRFEEVE EEPEVIPGPP SESPGMLTKL QIKQMMEAAT
160 170 180 190 200
RQIEERKKQL SFISPPAPQP KTPSSSQPER LPIGNTIQPS QAATFMNDAI
210 220 230 240 250
EKARKAAELQ ARIQAQLALK PGLIGNANMV GLANLHAMGI APPKVELKDQ
260 270 280 290 300
TKPTPLILDE QGRTVDATGK EVELTHRMPT LKANIRAVKR EQFKQQLKEK
310 320 330 340 350
PSEDMESNTF FDPRVSIAPS QRQRRTFKFH DKGKFEKIAQ RLRTKAQLEK
360 370 380 390 400
LQAEISQAAR KTGIHTSTRL ALIAPKKELK EGDIPEIEWW DSYIIPNGFD
410 420 430 440 450
LTEENPKRED YFGITNLVEH PAQLNPPVDN DTPVTLGVYL TKKEQKKLRR
460 470 480 490 500
QTRREAQKEL QEKVRLGLTP PPEPKVRISN LMRVLGTEAV QDPTKVEAHV
510 520 530 540 550
RAQMAKRQKA HEEANAARKL TAEQRKVKKI KKLKEDISQG VHISVYRVRN
560 570 580 590 600
LSNPAKKFKI EANAGQLYLT GVVVLHKDVN VVVVEGGPKA QKKFKRLMLH
610 620 630 640 650
RIKWDEQTSN TKGDDDEESD EEAVKKTNKC VLVWEGTAKD RSFGEMKFKQ
660 670 680
CPTENMAREH FKKHGAEHYW DLALSESVLE STD
Length:683
Mass (Da):77,455
Last modified:December 1, 2001 - v1
Checksum:i988219CF88E0EC4A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti78 – 781H → L in BAB29324 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014398 mRNA. Translation: BAB29324.1.
AK088194 mRNA. Translation: BAC40202.1.
AK167435 mRNA. Translation: BAE39521.1.
BC006782 mRNA. Translation: AAH06782.1.
CCDSiCCDS17621.1.
RefSeqiNP_081817.2. NM_027541.4.
XP_006502142.1. XM_006502079.1.
UniGeneiMm.279872.

Genome annotation databases

EnsembliENSMUST00000015892; ENSMUSP00000015892; ENSMUSG00000015748.
ENSMUST00000161476; ENSMUSP00000124950; ENSMUSG00000015748.
GeneIDi70767.
KEGGimmu:70767.
UCSCiuc008qlf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014398 mRNA. Translation: BAB29324.1.
AK088194 mRNA. Translation: BAC40202.1.
AK167435 mRNA. Translation: BAE39521.1.
BC006782 mRNA. Translation: AAH06782.1.
CCDSiCCDS17621.1.
RefSeqiNP_081817.2. NM_027541.4.
XP_006502142.1. XM_006502079.1.
UniGeneiMm.279872.

3D structure databases

ProteinModelPortaliQ922U1.
SMRiQ922U1. Positions 1-73.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ922U1. 2 interactions.
MINTiMINT-4108680.
STRINGi10090.ENSMUSP00000015892.

PTM databases

PhosphoSiteiQ922U1.

Proteomic databases

MaxQBiQ922U1.
PaxDbiQ922U1.
PRIDEiQ922U1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015892; ENSMUSP00000015892; ENSMUSG00000015748.
ENSMUST00000161476; ENSMUSP00000124950; ENSMUSG00000015748.
GeneIDi70767.
KEGGimmu:70767.
UCSCiuc008qlf.1. mouse.

Organism-specific databases

CTDi9129.
MGIiMGI:1918017. Prpf3.

Phylogenomic databases

eggNOGiNOG268901.
GeneTreeiENSGT00390000011497.
HOGENOMiHOG000205412.
HOVERGENiHBG053699.
InParanoidiQ922U1.
KOiK12843.
OMAiPWVERTV.
OrthoDBiEOG789C9P.
PhylomeDBiQ922U1.
TreeFamiTF313082.

Miscellaneous databases

NextBioi332211.
PROiQ922U1.
SOURCEiSearch...

Gene expression databases

BgeeiQ922U1.
CleanExiMM_PRPF3.
ExpressionAtlasiQ922U1. baseline and differential.
GenevestigatoriQ922U1.

Family and domain databases

Gene3Di1.20.1390.10. 1 hit.
InterProiIPR010541. DUF1115.
IPR013881. Pre-mRNA_splic_Prp3.
IPR027104. Prp3.
IPR002483. PWI_dom.
[Graphical view]
PANTHERiPTHR14212. PTHR14212. 1 hit.
PfamiPF06544. DUF1115. 1 hit.
PF08572. PRP3. 1 hit.
PF01480. PWI. 1 hit.
[Graphical view]
SMARTiSM00311. PWI. 1 hit.
[Graphical view]
SUPFAMiSSF101233. SSF101233. 1 hit.
PROSITEiPS51025. PWI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Placenta and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.

Entry informationi

Entry nameiPRPF3_MOUSE
AccessioniPrimary (citable) accession number: Q922U1
Secondary accession number(s): Q3TJH4, Q9D6C6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: December 1, 2001
Last modified: March 4, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.