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Q922S8

- KIF2C_MOUSE

UniProt

Q922S8 - KIF2C_MOUSE

Protein

Kinesin-like protein KIF2C

Gene

Kif2c

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei260 – 2601ATP

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi344 – 3518ATP

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. microtubule motor activity Source: InterPro
    3. microtubule plus-end binding Source: UniProtKB

    GO - Biological processi

    1. chromosome segregation Source: UniProtKB-KW
    2. establishment or maintenance of microtubule cytoskeleton polarity Source: Ensembl
    3. microtubule-based movement Source: InterPro
    4. microtubule depolymerization Source: Ensembl
    5. mitotic nuclear division Source: UniProtKB-KW
    6. regulation of chromosome segregation Source: Ensembl

    Keywords - Biological processi

    Cell cycle, Cell division, Chromosome partition, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_196550. MHC class II antigen presentation.
    REACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_199117. Kinesins.
    REACT_207679. Separation of Sister Chromatids.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kinesin-like protein KIF2C
    Alternative name(s):
    Mitotic centromere-associated kinesin
    Short name:
    MCAK
    Gene namesi
    Name:Kif2c
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:1921054. Kif2c.

    Subcellular locationi

    Cytoplasmcytoskeleton By similarity. Nucleus By similarity. Chromosomecentromere By similarity. Chromosomecentromerekinetochore By similarity
    Note: Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1. Association with microtubule plus ends is also mediated by interaction with KIF18B By similarity. Centromeric localization requires the presence of BUB1 and SGOL2 By similarity.By similarity

    GO - Cellular componenti

    1. chromosome, centromeric region Source: MGI
    2. condensed chromosome kinetochore Source: UniProtKB-SubCell
    3. cytoplasm Source: UniProtKB-KW
    4. kinesin complex Source: InterPro
    5. microtubule plus-end Source: Ensembl
    6. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi293 – 2931K → A: Loss of microtubule depolymerization activity. 1 Publication
    Mutagenesisi294 – 2941V → A: Loss of microtubule depolymerization activity. 1 Publication
    Mutagenesisi295 – 2951D → A: Loss of microtubule depolymerization activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 721721Kinesin-like protein KIF2CPRO_0000125420Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei19 – 191PhosphoserineBy similarity
    Modified residuei92 – 921Phosphoserine; by AURKBBy similarity
    Modified residuei106 – 1061PhosphoserineBy similarity
    Modified residuei108 – 1081PhosphoserineBy similarity
    Modified residuei112 – 1121PhosphoserineBy similarity
    Modified residuei162 – 1621PhosphoserineBy similarity
    Modified residuei188 – 1881PhosphoserineBy similarity
    Modified residuei626 – 6261PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylation by AURKB, regulates association with centromeres and kinetochores and the microtubule depolymerization activity.By similarity
    Ubiquitinated.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ922S8.
    PRIDEiQ922S8.

    PTM databases

    PhosphoSiteiQ922S8.

    Expressioni

    Gene expression databases

    ArrayExpressiQ922S8.
    BgeeiQ922S8.
    CleanExiMM_KIF2C.
    GenevestigatoriQ922S8.

    Interactioni

    Subunit structurei

    Interacts with CENPH. Interacts with MTUS2/TIP150; the interaction is direct. Interacts with MAPRE1; the interaction is direct, regulated by phosphorylation and is probably required for targeting to growing microtubule plus ends By similarity. Interacts with KIF18B at microtubule tips; this interaction increases the affinity of both partners for microtubule plus ends and is required for robust microtubule depolymerization. Phosphorylation by AURKA or AURKB strongly reduces KIF18B-binding By similarity.By similarity

    Protein-protein interaction databases

    BioGridi216265. 2 interactions.

    Structurei

    Secondary structure

    1
    721
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi226 – 23914
    Turni243 – 2475
    Beta strandi255 – 2617
    Helixi266 – 2705
    Beta strandi281 – 29212
    Beta strandi298 – 3069
    Beta strandi308 – 3114
    Helixi317 – 3237
    Helixi326 – 3283
    Helixi329 – 3335
    Beta strandi337 – 3459
    Helixi350 – 3556
    Helixi365 – 3673
    Helixi369 – 38113
    Helixi384 – 3874
    Beta strandi392 – 40110
    Beta strandi404 – 4074
    Turni408 – 4125
    Beta strandi414 – 4196
    Beta strandi421 – 4233
    Beta strandi425 – 4284
    Beta strandi433 – 4375
    Helixi438 – 45013
    Beta strandi465 – 48824
    Helixi506 – 5083
    Helixi509 – 52618
    Helixi539 – 5435
    Helixi546 – 5494
    Beta strandi550 – 56112
    Helixi565 – 5673
    Helixi568 – 58215

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1V8JX-ray3.24A183-585[»]
    1V8KX-ray2.25A183-585[»]
    ProteinModelPortaliQ922S8.
    SMRiQ922S8. Positions 224-585.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ922S8.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini254 – 584331Kinesin motorPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 250250GlobularSequence AnalysisAdd
    BLAST
    Regioni203 – 23432Negative regulator of microtubule-bindingAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili614 – 65239Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi95 – 984Microtubule tip localization signal

    Domaini

    The microtubule tip localization signal (MtLS) motif; mediates interaction with MAPRE1 and targeting to the growing microtubule plus ends.By similarity

    Sequence similaritiesi

    Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. MCAK/KIF2 subfamily.PROSITE-ProRule annotation
    Contains 1 kinesin motor domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5059.
    GeneTreeiENSGT00740000114912.
    HOGENOMiHOG000231329.
    HOVERGENiHBG003875.
    InParanoidiA2AE71.
    KOiK10393.
    OMAiDETASNE.
    OrthoDBiEOG7K9K2C.
    PhylomeDBiQ922S8.
    TreeFamiTF105222.

    Family and domain databases

    Gene3Di3.40.850.10. 1 hit.
    InterProiIPR027640. Kinesin-like_fam.
    IPR019821. Kinesin_motor_CS.
    IPR001752. Kinesin_motor_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR24115. PTHR24115. 1 hit.
    PfamiPF00225. Kinesin. 1 hit.
    [Graphical view]
    PRINTSiPR00380. KINESINHEAVY.
    SMARTiSM00129. KISc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
    PS50067. KINESIN_MOTOR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q922S8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESLHARLFP GLSINIQRSN GLIHPANIST VNVEKSCVSV EWIEGGTTKG    50
    KEIDIDDVAA INPELLQLLP LRPKDSLPLQ ENVTVPKQKR KSVNSKIPAL 100
    KEGLRSRSTR MSTVSEVRIP AQENEMEVEL PVPTNSRKQF AIPSHPRASC 150
    STVTELPLLM VSEEAEEQAH STRSTSSANP GNSVRRKSCI VKEMEKMKNK 200
    REEKRAQNSE LRIKRAQEYD SSFPNWEFAR MIKEFRVTME CSPLTVTDPI 250
    EEHRICVCVR KRPLNKQELA KKEIDVISVP SKCLLLVHEP KLKVDLTKYL 300
    ENQAFCFDFA FDETASNEVV YRFTARPLVQ TIFEGGKATC FAYGQTGSGK 350
    THTMGGDLSG KSQNASKGIY AMASRDVFLL KNQPRYRNLN LEVYVTFFEI 400
    YNGKVFDLLN KKAKLRVLED SRQQVQVVGL QEYLVTCADD VIKMINMGSA 450
    CRTSGQTFAN SNSSRSHACF QILLRTKGRL HGKFSLVDLA GNERGADTSS 500
    ADRQTRMEGA EINKSLLALK ECIRALGQNK AHTPFRESKL TQVLRDSFIG 550
    ENSRTCMIAM ISPGISSCEY TLNTLRYADR VKELSPHSGP SGEQPVQMET 600
    EVMEASSNGT SLTGNEEEEL SSQMSSFNEA MTQIRELEER ALEELREIIQ 650
    QGPNWLELSE MTDQPDYDLE TFVNKAESAL TQQAKQAKHF SALREVIKAL 700
    RLAMQLEEQA SKQINSKKRH Q 721
    Length:721
    Mass (Da):81,085
    Last modified:December 1, 2001 - v1
    Checksum:i92FFBFFDA05B7E35
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL671866 Genomic DNA. Translation: CAM26318.1.
    BC006841 mRNA. Translation: AAH06841.1.
    CCDSiCCDS18531.1.
    RefSeqiNP_608301.3. NM_134471.4.
    UniGeneiMm.247651.

    Genome annotation databases

    EnsembliENSMUST00000065896; ENSMUSP00000064261; ENSMUSG00000028678.
    GeneIDi73804.
    KEGGimmu:73804.
    UCSCiuc008uie.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL671866 Genomic DNA. Translation: CAM26318.1 .
    BC006841 mRNA. Translation: AAH06841.1 .
    CCDSi CCDS18531.1.
    RefSeqi NP_608301.3. NM_134471.4.
    UniGenei Mm.247651.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1V8J X-ray 3.24 A 183-585 [» ]
    1V8K X-ray 2.25 A 183-585 [» ]
    ProteinModelPortali Q922S8.
    SMRi Q922S8. Positions 224-585.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 216265. 2 interactions.

    PTM databases

    PhosphoSitei Q922S8.

    Proteomic databases

    PaxDbi Q922S8.
    PRIDEi Q922S8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000065896 ; ENSMUSP00000064261 ; ENSMUSG00000028678 .
    GeneIDi 73804.
    KEGGi mmu:73804.
    UCSCi uc008uie.2. mouse.

    Organism-specific databases

    CTDi 11004.
    MGIi MGI:1921054. Kif2c.

    Phylogenomic databases

    eggNOGi COG5059.
    GeneTreei ENSGT00740000114912.
    HOGENOMi HOG000231329.
    HOVERGENi HBG003875.
    InParanoidi A2AE71.
    KOi K10393.
    OMAi DETASNE.
    OrthoDBi EOG7K9K2C.
    PhylomeDBi Q922S8.
    TreeFami TF105222.

    Enzyme and pathway databases

    Reactomei REACT_196550. MHC class II antigen presentation.
    REACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_199117. Kinesins.
    REACT_207679. Separation of Sister Chromatids.

    Miscellaneous databases

    EvolutionaryTracei Q922S8.
    NextBioi 339083.
    PROi Q922S8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q922S8.
    Bgeei Q922S8.
    CleanExi MM_KIF2C.
    Genevestigatori Q922S8.

    Family and domain databases

    Gene3Di 3.40.850.10. 1 hit.
    InterProi IPR027640. Kinesin-like_fam.
    IPR019821. Kinesin_motor_CS.
    IPR001752. Kinesin_motor_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR24115. PTHR24115. 1 hit.
    Pfami PF00225. Kinesin. 1 hit.
    [Graphical view ]
    PRINTSi PR00380. KINESINHEAVY.
    SMARTi SM00129. KISc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00411. KINESIN_MOTOR_1. 1 hit.
    PS50067. KINESIN_MOTOR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "A common mechanism for microtubule destabilizers-M type kinesins stabilize curling of the protofilament using the class-specific neck and loops."
      Ogawa T., Nitta R., Okada Y., Hirokawa N.
      Cell 116:591-602(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 183-585 IN COMPLEX WITH ADP AND ATP ANALOG, FUNCTION, MUTAGENESIS OF LYS-293; VAL-294 AND ASP-295.

    Entry informationi

    Entry nameiKIF2C_MOUSE
    AccessioniPrimary (citable) accession number: Q922S8
    Secondary accession number(s): A2AE71
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 28, 2003
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3