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Protein

Pyrroline-5-carboxylate reductase 2

Gene

Pycr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP+. Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH (By similarity). Involved in cellular response to oxidative stress (By similarity).By similarity

Catalytic activityi

L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H.

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-proline from L-glutamate 5-semialdehyde.
Proteins known to be involved in this subpathway in this organism are:
  1. Pyrroline-5-carboxylate reductase 3 (Pycrl), Pyrroline-5-carboxylate reductase 1, mitochondrial (Pycr1), Pyrroline-5-carboxylate reductase 2 (Pycr2), Pyrroline-5-carboxylate reductase (Pycr2), Pyrroline-5-carboxylate reductase (Pycr1), Pyrroline-5-carboxylate reductase (Pycr2)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-proline from L-glutamate 5-semialdehyde, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341NADP; via carbonyl oxygenBy similarity
Binding sitei56 – 561NADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi6 – 116NADPBy similarity
Nucleotide bindingi69 – 724NADPBy similarity
Nucleotide bindingi95 – 973NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiR-MMU-70614. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00098; UER00361.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrroline-5-carboxylate reductase 2 (EC:1.5.1.2)
Short name:
P5C reductase 2
Short name:
P5CR 2
Gene namesi
Name:Pycr2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1277956. Pycr2.

Subcellular locationi

  • Cytoplasm By similarity
  • Mitochondrion By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 320319Pyrroline-5-carboxylate reductase 2PRO_0000187318Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei304 – 3041PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ922Q4.
MaxQBiQ922Q4.
PaxDbiQ922Q4.
PRIDEiQ922Q4.

PTM databases

iPTMnetiQ922Q4.
PhosphoSiteiQ922Q4.
SwissPalmiQ922Q4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026520.
ExpressionAtlasiQ922Q4. baseline and differential.
GenevisibleiQ922Q4. MM.

Interactioni

Subunit structurei

Homodecamer; composed of 5 homodimers.By similarity

Protein-protein interaction databases

BioGridi213197. 2 interactions.
IntActiQ922Q4. 2 interactions.
MINTiMINT-1853710.
STRINGi10090.ENSMUSP00000027802.

Structurei

3D structure databases

ProteinModelPortaliQ922Q4.
SMRiQ922Q4. Positions 1-275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3124. Eukaryota.
COG0345. LUCA.
GeneTreeiENSGT00390000007443.
HOGENOMiHOG000230247.
HOVERGENiHBG053399.
InParanoidiQ922Q4.
KOiK00286.
OMAiAEQYGIN.
OrthoDBiEOG091G0KMI.
PhylomeDBiQ922Q4.

Family and domain databases

Gene3Di1.10.3730.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01925. P5C_reductase. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR029036. P5CR_dimer.
IPR028939. ProC_N.
IPR000304. Pyrroline-COOH_reductase.
[Graphical view]
PANTHERiPTHR11645. PTHR11645. 1 hit.
PfamiPF03807. F420_oxidored. 1 hit.
PF14748. P5CR_dimer. 1 hit.
[Graphical view]
PIRSFiPIRSF000193. Pyrrol-5-carb_rd. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00112. proC. 1 hit.
PROSITEiPS00521. P5CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q922Q4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVGFIGAGQ LACALARGFT AAGVLSAHKI IASSPDMDLP TVSALRRMGV
60 70 80 90 100
NLTRSNKDTV RHSDVLFLAV KPHIIPFILD EIGADVQERH IVVSCAAGVT
110 120 130 140 150
ISSVEKKLMA FQPAPKVIRC MTNTPVVVRE GATVYATGTH ALVEDGKLLE
160 170 180 190 200
QLMSSVGFCT EVEEDLIDAI TGLSGSGPAY AFMALDALAD GGVKMGVPRR
210 220 230 240 250
LAVRLGAQAL LGAAKMLLDS EDHPGQLKDN VCSPGGATIH ALHFLESGGF
260 270 280 290 300
RSLLINAVEA SCIRTRELQS MADQEKVSPA ALKKTLLDRV KLESPTVSTL
310 320
APPSSGKLLT RNPAQGSKKE
Length:320
Mass (Da):33,659
Last modified:December 1, 2001 - v1
Checksum:i64DD7F96FB8C4BC5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC006882 mRNA. Translation: AAH06882.1.
CCDSiCCDS15576.1.
RefSeqiNP_598466.1. NM_133705.2.
UniGeneiMm.274180.

Genome annotation databases

EnsembliENSMUST00000027802; ENSMUSP00000027802; ENSMUSG00000026520.
GeneIDi69051.
KEGGimmu:69051.
UCSCiuc007dwv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC006882 mRNA. Translation: AAH06882.1.
CCDSiCCDS15576.1.
RefSeqiNP_598466.1. NM_133705.2.
UniGeneiMm.274180.

3D structure databases

ProteinModelPortaliQ922Q4.
SMRiQ922Q4. Positions 1-275.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213197. 2 interactions.
IntActiQ922Q4. 2 interactions.
MINTiMINT-1853710.
STRINGi10090.ENSMUSP00000027802.

PTM databases

iPTMnetiQ922Q4.
PhosphoSiteiQ922Q4.
SwissPalmiQ922Q4.

Proteomic databases

EPDiQ922Q4.
MaxQBiQ922Q4.
PaxDbiQ922Q4.
PRIDEiQ922Q4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027802; ENSMUSP00000027802; ENSMUSG00000026520.
GeneIDi69051.
KEGGimmu:69051.
UCSCiuc007dwv.2. mouse.

Organism-specific databases

CTDi29920.
MGIiMGI:1277956. Pycr2.

Phylogenomic databases

eggNOGiKOG3124. Eukaryota.
COG0345. LUCA.
GeneTreeiENSGT00390000007443.
HOGENOMiHOG000230247.
HOVERGENiHBG053399.
InParanoidiQ922Q4.
KOiK00286.
OMAiAEQYGIN.
OrthoDBiEOG091G0KMI.
PhylomeDBiQ922Q4.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00361.
ReactomeiR-MMU-70614. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

ChiTaRSiPycr2. mouse.
PROiQ922Q4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026520.
ExpressionAtlasiQ922Q4. baseline and differential.
GenevisibleiQ922Q4. MM.

Family and domain databases

Gene3Di1.10.3730.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01925. P5C_reductase. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR029036. P5CR_dimer.
IPR028939. ProC_N.
IPR000304. Pyrroline-COOH_reductase.
[Graphical view]
PANTHERiPTHR11645. PTHR11645. 1 hit.
PfamiPF03807. F420_oxidored. 1 hit.
PF14748. P5CR_dimer. 1 hit.
[Graphical view]
PIRSFiPIRSF000193. Pyrrol-5-carb_rd. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00112. proC. 1 hit.
PROSITEiPS00521. P5CR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP5CR2_MOUSE
AccessioniPrimary (citable) accession number: Q922Q4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: December 1, 2001
Last modified: September 7, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.