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Protein

Serine/threonine-protein kinase RIO1

Gene

Riok1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in processing of 18S-E pre-rRNA to the mature 18S rRNA. Required for the recycling of NOB1 and PNO1 from the late 40S precursor (By similarity). The association with the very late 40S subunit intermediate may involve a translation-like checkpoint point cycle preceeding the binding to the 60S ribosomal subunit (By similarity). Despite the protein kinase domain is proposed to act predominantly as an ATPase (By similarity). The catalytic activity regulates its dynamic association with the 40S subunit (By similarity). In addition to its role in ribosomal biogenesis acts as an adapter protein by recruiting NCL/nucleolin the to PRMT5 complex for its symmetrical methylation (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarityCurated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei207ATPBy similarity1
Binding sitei279ATP; via amide nitrogenBy similarity1
Active sitei323Proton acceptorBy similarity1
Metal bindingi328MagnesiumBy similarity1
Binding sitei328ATPBy similarity1
Active sitei3404-aspartylphosphate intermediateBy similarity1
Metal bindingi340MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Kinase, Serine/threonine-protein kinase, Transferase
Biological processRibosome biogenesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase RIO1 (EC:2.7.11.1By similarity, EC:3.6.3.-)
Alternative name(s):
RIO kinase 1
Gene namesi
Name:Riok1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1918590 Riok1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003144901 – 567Serine/threonine-protein kinase RIO1Add BLAST567

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei21PhosphoserineCombined sources1
Modified residuei22PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ922Q2
MaxQBiQ922Q2
PaxDbiQ922Q2
PeptideAtlasiQ922Q2
PRIDEiQ922Q2

PTM databases

iPTMnetiQ922Q2
PhosphoSitePlusiQ922Q2

Expressioni

Gene expression databases

BgeeiENSMUSG00000021428
CleanExiMM_RIOK1
GenevisibleiQ922Q2 MM

Interactioni

Subunit structurei

Associates with the precursor of the 40S ribosome subunit. Interacts (via its N-terminus) with PRMT5 (via its N-terminus). Interacts with WDR77 (By similarity). Found in a PRMT5 complex composed of PRMT5, WDR77 and RIOK1 (By similarity). Interacts (via its C-terminus) with NCL; this interaction targets NCL for PRTM5 methylation (By similarity).By similarity

Protein-protein interaction databases

IntActiQ922Q2, 1 interactor
STRINGi10090.ENSMUSP00000021866

Structurei

3D structure databases

ProteinModelPortaliQ922Q2
SMRiQ922Q2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini179 – 478Protein kinaseAdd BLAST300

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2270 Eukaryota
COG1718 LUCA
GeneTreeiENSGT00390000004370
HOGENOMiHOG000166501
HOVERGENiHBG056997
InParanoidiQ922Q2
KOiK07178
OMAiQEINGCI
OrthoDBiEOG091G064C
PhylomeDBiQ922Q2
TreeFamiTF105831

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000687 RIO_kinase
IPR018935 RIO_kinase_CS
IPR017407 Ser/Thr_kinase_Rio1
PIRSFiPIRSF038147 Ser/Thr_PK_RIO1, 1 hit
SMARTiView protein in SMART
SM00090 RIO, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01245 RIO1, 1 hit

Sequencei

Sequence statusi: Complete.

Q922Q2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDCSLDRMAS VVPGQFDDAD SSDSENKELQ PIHAEDGGVL LKSLQNAAAE
60 70 80 90 100
VKGDAETDEE DDYDDDDDWY LDDATGKLTK GCTWNGGSNY QANRQTSNYN
110 120 130 140 150
SAKMSTPIDK SLRKFENKIN LNKLNVTDSV TNKVTVKLRQ KEAESYRIKD
160 170 180 190 200
KADRATVEQV LDPRTRMILF KLLHKDHISE IHGCISTGKE ANVYYASTPS
210 220 230 240 250
GESRAIKIYK TSILMFKDRD KYVTGEFRFR RGYCKGNPRK MVRTWAEKEM
260 270 280 290 300
RNLCRLKTAN IPCPEPIRLR SHVLLMGFIG KDDMPAPLLK NVQLSESKAR
310 320 330 340 350
ELYLQVIQYM RKMYQDARLV HADLSEFNML YHGGDVYIID VSQSVEHDHP
360 370 380 390 400
HALEFLRKDC TNVNDFFSKH AVAVMTVREL FDFVTDPSIT ADNMDAYLEK
410 420 430 440 450
AMEIASQRTK EEKTSQDHVD EEVFKQAYIP RTLNEVKNYE RDVDIMMRLK
460 470 480 490 500
EEDMALNTQQ DNILYQTVMG LKKDLSGVQK VPALLESEVK EETCFGSDDA
510 520 530 540 550
GGSECSDTVS EEQEDQAGCR NHIADPDIDK KERKKMVKEA QREKRKNKIP
560
KHVKKRKEKT AKAKKGK
Length:567
Mass (Da):64,910
Last modified:January 15, 2008 - v2
Checksum:iD2A1863DF0132366
GO

Sequence cautioni

The sequence AAH02158 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB29195 differs from that shown. Intron retention.Curated
The sequence BAB29195 differs from that shown. Reason: Frameshift at position 434.Curated
The sequence BAB29195 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB30687 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti436V → E in BAB29195 (PubMed:16141072).Curated1
Sequence conflicti492E → K in BAB29195 (PubMed:16141072).Curated1
Sequence conflicti528I → V in AAH02158 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014182 mRNA Translation: BAB29195.1 Sequence problems.
AK017312 mRNA Translation: BAB30687.2 Different initiation.
AK152707 mRNA Translation: BAE31434.1
AC140331 Genomic DNA No translation available.
CT010477 Genomic DNA No translation available.
BC002158 mRNA Translation: AAH02158.1 Different initiation.
CCDSiCCDS26461.1
RefSeqiNP_077204.2, NM_024242.3
UniGeneiMm.297130

Genome annotation databases

EnsembliENSMUST00000021866; ENSMUSP00000021866; ENSMUSG00000021428
GeneIDi71340
KEGGimmu:71340
UCSCiuc007qdi.2 mouse

Similar proteinsi

Entry informationi

Entry nameiRIOK1_MOUSE
AccessioniPrimary (citable) accession number: Q922Q2
Secondary accession number(s): Q3U7D5
, Q99LZ1, Q9CU84, Q9CXN9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: May 23, 2018
This is version 94 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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