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Protein

Putative oxidoreductase GLYR1

Gene

Glyr1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Putative oxidoreductase that is recruited on chromatin and promotes KDM1B demethylase activity. Recognizes and binds trimethylated 'Lys-36' of histone H3 (H3K36me3). Regulates p38 MAP kinase activity by mediating stress activation of p38alpha/MAPK14 and specifically regulating MAPK14 signaling. Indirectly promotes phosphorylation of MAPK14 and activation of ATF2. The phosphorylation of MAPK14 requires upstream activity of MAP2K4 and MAP2K6.By similarity

Miscellaneous

The conserved NAD-binding sites and sequence similarity to plant dehydrogenases suggest that this protein may have oxidoreductase activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei216Required to promote KDM1B demethylase activity toward histone H3K4me1 and H3K4me2By similarity1
Binding sitei355NADBy similarity1
Binding sitei498NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi167 – 179A.T hookCuratedAdd BLAST13
Nucleotide bindingi270 – 284NADBy similarityAdd BLAST15

GO - Molecular functioni

Keywordsi

Molecular functionDNA-binding, Oxidoreductase
LigandNAD

Names & Taxonomyi

Protein namesi
Recommended name:
Putative oxidoreductase GLYR1 (EC:1.-.-.-)
Alternative name(s):
Glyoxylate reductase 1 homolog
Nuclear protein NP60
Gene namesi
Name:Glyr1
Synonyms:Np60
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1921272 Glyr1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003121221 – 546Putative oxidoreductase GLYR1Add BLAST546

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei130PhosphoserineBy similarity1
Cross-linki135Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei166PhosphoserineBy similarity1
Cross-linki175Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki178Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki200Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki210Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki226Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki236Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki239Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki268Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki301Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei533PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ922P9
MaxQBiQ922P9
PaxDbiQ922P9
PeptideAtlasiQ922P9
PRIDEiQ922P9

PTM databases

iPTMnetiQ922P9
PhosphoSitePlusiQ922P9

Expressioni

Gene expression databases

BgeeiENSMUSG00000022536
CleanExiMM_3930401K13RIK
ExpressionAtlasiQ922P9 baseline and differential
GenevisibleiQ922P9 MM

Interactioni

Subunit structurei

Interacts with MAPK14. Interacts with KDM1B at nucleosomes; this interaction stimulates H3K4me1 and H3K4me2 demethylation.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi2164311 interactor.
IntActiQ922P9 3 interactors.
MINTiQ922P9
STRINGi10090.ENSMUSP00000111510

Structurei

3D structure databases

ProteinModelPortaliQ922P9
SMRiQ922P9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 66PWWPPROSITE-ProRule annotationAdd BLAST59

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni213 – 216Interaction with histone H3By similarity4
Regioni215 – 224Interaction with KDM1BBy similarity10

Domaini

The A.T hook DNA-binding domain is required for the interaction with MAPK14.By similarity
The PWWP domain probably mediates the binding to H3K36me3.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0409 Eukaryota
KOG1904 Eukaryota
COG2084 LUCA
GeneTreeiENSGT00530000063270
HOGENOMiHOG000219609
InParanoidiQ922P9

Family and domain databases

CDDicd05836 N_Pac_NP60, 1 hit
Gene3Di1.10.1040.101 hit
InterProiView protein in InterPro
IPR008927 6-PGluconate_DH-like_C_sf
IPR013328 6PGD_dom2
IPR006115 6PGDH_NADP-bd
IPR036291 NAD(P)-bd_dom_sf
IPR029154 NADP-bd
IPR035501 NP60_PWWP
IPR000313 PWWP_dom
PfamiView protein in Pfam
PF14833 NAD_binding_11, 1 hit
PF03446 NAD_binding_2, 1 hit
PF00855 PWWP, 1 hit
SMARTiView protein in SMART
SM00293 PWWP, 1 hit
SUPFAMiSSF48179 SSF48179, 1 hit
SSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS50812 PWWP, 1 hit

Sequencei

Sequence statusi: Complete.

Q922P9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVSLRLGD LVWGKLGRYP PWPGKIVNPP KDLKKPRGKK CFFVKFFGTE
60 70 80 90 100
DHAWIKVEQL KPYHAHKEEM IKINKGKRFQ QAVDAVEEFL RRAKGKDQTS
110 120 130 140 150
SHTSADDKNR RNSSEERSRP NSGDEKRKLS LSEGKVKKNM GEGKKRVTSG
160 170 180 190 200
SADRGSKCLK RAQEQSPRKR GRPPKDEKDL TIPESSTVKG MMAGPMAAFK
210 220 230 240 250
WQPTATEPVK DADPHFHHFL LSQTEKPAVC YQAITKKLKI CEEETGSTSI
260 270 280 290 300
QAADSTAVNG SITPTDKKIG FLGLGLMGSG IVSNLLKMGH TVTVWNRTAE
310 320 330 340 350
KEGARLGRTP AEVVSTCDIT FACVSDPKAA KDLVLGPSGV LQGIRPGKCY
360 370 380 390 400
VDMSTVDADT VTELAQVIVS RGGRFLEAPV SGNQQLSNDG MLVILAAGDR
410 420 430 440 450
GLYEDCSSCF QAMGKTSFFL GEVGNAAKMM LIVNMVQGSF MATIAEGLTL
460 470 480 490 500
AQVTGQSQQT LLDILNQGQL ASIFLDQKCQ NILQGNFKPD FYLKYIQKDL
510 520 530 540
RLAIALGDAV NHPTPMAAAA NEVYKRAKAL DQSDNDMSAV YRAYIH
Length:546
Mass (Da):59,716
Last modified:December 1, 2001 - v1
Checksum:iF5D2090DE1F64723
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50 – 51ED → KN in BAB29363 (PubMed:16141072).Curated2
Sequence conflicti109 – 110NR → DW in BAB29363 (PubMed:16141072).Curated2
Sequence conflicti296N → S in BAE35114 (PubMed:16141072).Curated1
Sequence conflicti535N → S in BAE35114 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014456 mRNA Translation: BAB29363.1
AK150349 mRNA Translation: BAE29486.1
AK152887 mRNA Translation: BAE31570.1
AK159476 mRNA Translation: BAE35114.1
BC006893 mRNA Translation: AAH06893.1
CCDSiCCDS27930.1
RefSeqiNP_082996.2, NM_028720.2
UniGeneiMm.21652

Genome annotation databases

EnsembliENSMUST00000023189; ENSMUSP00000023189; ENSMUSG00000022536
GeneIDi74022
KEGGimmu:74022
UCSCiuc007ybm.1 mouse

Similar proteinsi

Entry informationi

Entry nameiGLYR1_MOUSE
AccessioniPrimary (citable) accession number: Q922P9
Secondary accession number(s): Q3TX02, Q9CYQ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: December 1, 2001
Last modified: February 28, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome