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Q922K9

- FRK_MOUSE

UniProt

Q922K9 - FRK_MOUSE

Protein

Tyrosine-protein kinase FRK

Gene

Frk

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor By similarity.By similarity

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei269 – 2691ATPPROSITE-ProRule annotation
    Active sitei361 – 3611Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi247 – 2559ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cell differentiation Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein kinase FRK (EC:2.7.10.2)
    Alternative name(s):
    Beta-cell Src-homology tyrosine kinase
    Short name:
    BSK
    FYN-related kinase
    Intestine tyrosine kinase
    Gene namesi
    Name:Frk
    Synonyms:Bsk, Iyk
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:103265. Frk.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity
    Note: Predominantly found in the nucleus, with a small fraction found in the cell periphery.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Mice are viable and do not show any histological abnormalities in epithelial tissues or develop any pathological and/or metabolic disorders associated with the failure of epithelial organs.1 Publication

    Keywords - Diseasei

    Tumor suppressor

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 512512Tyrosine-protein kinase FRKPRO_0000260826Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei394 – 3941Phosphotyrosine; by autocatalysis1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ922K9.
    PaxDbiQ922K9.
    PRIDEiQ922K9.

    PTM databases

    PhosphoSiteiQ922K9.

    Expressioni

    Tissue specificityi

    Expressed in intestinal tract, fetal and adult islets of Langerhans, kidney, liver and lung.2 Publications

    Gene expression databases

    BgeeiQ922K9.
    CleanExiMM_FRK.
    GenevestigatoriQ922K9.

    Interactioni

    Subunit structurei

    Interacts (via the SH3-domain) with PTEN. Interacts with RB1 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000019913.

    Structurei

    Secondary structure

    1
    512
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi52 – 587
    Beta strandi63 – 675
    Beta strandi76 – 805
    Beta strandi83 – 919
    Beta strandi103 – 1086
    Turni109 – 1113
    Beta strandi112 – 1143
    Helixi116 – 1205
    Beta strandi122 – 1243
    Helixi130 – 1378
    Beta strandi138 – 1414
    Beta strandi147 – 1515
    Beta strandi153 – 1575
    Beta strandi159 – 1679
    Beta strandi169 – 1768
    Beta strandi178 – 1803
    Beta strandi182 – 1854
    Beta strandi188 – 1925
    Helixi193 – 2008
    Beta strandi205 – 2095

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2D8JNMR-A52-115[»]
    2DLYNMR-A113-220[»]
    ProteinModelPortaliQ922K9.
    SMRiQ922K9. Positions 49-510.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ922K9.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini42 – 11776SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini123 – 21593SH2PROSITE-ProRule annotationAdd
    BLAST
    Domaini241 – 498258Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 SH2 domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH2 domain, SH3 domain

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00640000091347.
    HOGENOMiHOG000233858.
    HOVERGENiHBG008761.
    InParanoidiQ922K9.
    KOiK08892.
    OMAiVSHYTKT.
    OrthoDBiEOG7GTT2V.
    PhylomeDBiQ922K9.
    TreeFamiTF351634.

    Family and domain databases

    Gene3Di3.30.505.10. 1 hit.
    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    PRINTSiPR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTiSM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q922K9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSVCVRLWA YLQPFLPCWS QEADKSVVIE NPGAFCPPEA PRSQEPERSH    50
    GQYFVALFDY QARTAEDLSF RAGDKLQVLD TSHEGWWLAR HLEKKGTGLG 100
    QQLQGYIPSN YVAEDRSLQA EPWFFGAIKR ADAEKQLLYS ENQTGAFLIR 150
    ESESQKGDFS LSVLDEGVVK HYRIRRLDEG GFFLTRRKVF STLNEFVNYY 200
    TTTSDGLCVK LEKPCLKIQV PTPFDLSYKT ADQWEIDRNS IQLLKRLGSG 250
    QFGEVWEGLW NNTTPVAVKT LKPGSMDPND FLREAQIMKS LRHPKLIQLY 300
    AVCTLEDPIY IITELMRHGS LQEYLQNDGG SKIHLIQQVD MAAQVASGMA 350
    YLESQNYIHR DLAARNVLVG EHNIYKVADF GLARVFKVDN EDIYESKHEI 400
    KLPVKWTAPE AIRTNKFSIK SDVWSFGILL YEIITYGKMP YSGMTGAQVI 450
    QMLSQNYRLP QPSNCPQQFY SIMLECWNVE PKQRPTFETL HWKLEDYFET 500
    DCSYSDTNNF IN 512
    Length:512
    Mass (Da):58,844
    Last modified:January 23, 2007 - v3
    Checksum:iB79A0BC07B9EC5F8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti154 – 1541S → T in CAA88658. (PubMed:7733928)Curated
    Sequence conflicti237 – 2371D → H in CAA88658. (PubMed:7733928)Curated
    Sequence conflicti335 – 3351L → F in CAA88658. (PubMed:7733928)Curated
    Sequence conflicti335 – 3351L → F in AAA65197. (PubMed:7835707)Curated
    Sequence conflicti509 – 5091N → K in CAA88658. (PubMed:7733928)Curated
    Sequence conflicti509 – 5091N → K in AAA65197. (PubMed:7835707)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48757 mRNA. Translation: CAA88658.1.
    L36132 mRNA. Translation: AAA65197.1.
    AK028855 mRNA. Translation: BAC26155.1.
    AK052614 mRNA. Translation: BAC35063.1.
    AK166379 mRNA. Translation: BAE38741.1.
    BC007137 mRNA. Translation: AAH07137.1.
    CCDSiCCDS23781.1.
    PIRiI49552.
    RefSeqiNP_001153016.1. NM_001159544.1.
    NP_034367.2. NM_010237.3.
    XP_006512597.1. XM_006512534.1.
    XP_006512598.1. XM_006512535.1.
    XP_006512599.1. XM_006512536.1.
    UniGeneiMm.332432.

    Genome annotation databases

    EnsembliENSMUST00000019913; ENSMUSP00000019913; ENSMUSG00000019779.
    ENSMUST00000170771; ENSMUSP00000130289; ENSMUSG00000019779.
    GeneIDi14302.
    KEGGimmu:14302.
    UCSCiuc007euy.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48757 mRNA. Translation: CAA88658.1 .
    L36132 mRNA. Translation: AAA65197.1 .
    AK028855 mRNA. Translation: BAC26155.1 .
    AK052614 mRNA. Translation: BAC35063.1 .
    AK166379 mRNA. Translation: BAE38741.1 .
    BC007137 mRNA. Translation: AAH07137.1 .
    CCDSi CCDS23781.1.
    PIRi I49552.
    RefSeqi NP_001153016.1. NM_001159544.1.
    NP_034367.2. NM_010237.3.
    XP_006512597.1. XM_006512534.1.
    XP_006512598.1. XM_006512535.1.
    XP_006512599.1. XM_006512536.1.
    UniGenei Mm.332432.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2D8J NMR - A 52-115 [» ]
    2DLY NMR - A 113-220 [» ]
    ProteinModelPortali Q922K9.
    SMRi Q922K9. Positions 49-510.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000019913.

    PTM databases

    PhosphoSitei Q922K9.

    Proteomic databases

    MaxQBi Q922K9.
    PaxDbi Q922K9.
    PRIDEi Q922K9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000019913 ; ENSMUSP00000019913 ; ENSMUSG00000019779 .
    ENSMUST00000170771 ; ENSMUSP00000130289 ; ENSMUSG00000019779 .
    GeneIDi 14302.
    KEGGi mmu:14302.
    UCSCi uc007euy.2. mouse.

    Organism-specific databases

    CTDi 2444.
    MGIi MGI:103265. Frk.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00640000091347.
    HOGENOMi HOG000233858.
    HOVERGENi HBG008761.
    InParanoidi Q922K9.
    KOi K08892.
    OMAi VSHYTKT.
    OrthoDBi EOG7GTT2V.
    PhylomeDBi Q922K9.
    TreeFami TF351634.

    Miscellaneous databases

    EvolutionaryTracei Q922K9.
    NextBioi 285723.
    PROi Q922K9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q922K9.
    CleanExi MM_FRK.
    Genevestigatori Q922K9.

    Family and domain databases

    Gene3Di 3.30.505.10. 1 hit.
    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTi SM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "iyk, a novel intracellular protein tyrosine kinase differentially expressed in the mouse mammary gland and intestine."
      Thuveson M., Albrecht D., Zuercher G., Andres A., Ziemiecki A.
      Biochem. Biophys. Res. Commun. 209:582-589(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: BALB/c.
      Tissue: Mammary gland.
    2. "Cloning of BSK, a murine FRK homologue with a specific pattern of tissue distribution."
      Oberg-Welsh C., Welsh M.
      Gene 152:239-242(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Kidney, Mammary gland and Skin.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    5. "Characterization of mice deficient in the Src family nonreceptor tyrosine kinase Frk/rak."
      Chandrasekharan S., Qiu T.H., Alkharouf N., Brantley K., Mitchell J.B., Liu E.T.
      Mol. Cell. Biol. 22:5235-5247(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    6. "The tyrosine kinase FRK/RAK participates in cytokine-induced islet cell cytotoxicity."
      Welsh M., Welsh C., Ekman M., Dixelius J., Hagerkvist R., Anneren C., Akerblom B., Mahboobi S., Chandrasekharan S., Liu E.T.
      Biochem. J. 382:261-268(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT TYR-394.
    7. "Solution structure of the SH3 and SH2 domains of FYN-related kinase."
      RIKEN structural genomics initiative (RSGI)
      Submitted (OCT-2006) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 50-220.

    Entry informationi

    Entry nameiFRK_MOUSE
    AccessioniPrimary (citable) accession number: Q922K9
    Secondary accession number(s): Q61364, Q61745
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2006
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 111 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3