Reviewed, UniProtKB/Swiss-Prot Q922K9 (FRK_MOUSE)

Names and origin

Protein names

Recommended name:
    Tyrosine-protein kinase FRK
    EC=2.7.10.2
Alternative name(s):
    FYN-related kinase
    Beta-cell Src-homology tyrosine kinase
      Short name=BSK
    Intestine tyrosine kinase

Gene names

Name:	Frk
Synonyms:	Bsk, Iyk

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation possibly by reducing its binding to NEDD4. May function as a tumor suppressor By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	Interacts (via the SH3-domain) with PTEN By similarity.
Subcellular location	Cytoplasm Probable.
Tissue specificity	Expressed in intestinal tract, fetal and adult islets of Langerhans, kidney, liver and lung. Ref.1 Ref.2
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Disease	Tumor suppressor
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Lipoprotein Myristate Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 512	511	Tyrosine-protein kinase FRK		PRO_0000260826
Regions
	Domain	42 – 117	76	SH3
	Domain	123 – 215	93	SH2
	Domain	241 – 498	258	Protein kinase
	Nucleotide binding	247 – 255	9	ATP By similarity
Sites
	Active site	361	1	Proton acceptor By similarity
	Binding site	269	1	ATP By similarity
Amino acid modifications
	Modified residue	53	1	Phosphotyrosine By similarity
	Modified residue	69	1	Phosphoserine By similarity
	Modified residue	394	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	504	1	Phosphotyrosine By similarity
	Lipidation	2	1	N-myristoyl glycine By similarity
Experimental info
	Sequence conflict	154	1	S → T in CAA88658. Ref.1
	Sequence conflict	237	1	D → H in CAA88658. Ref.1
	Sequence conflict	335	1	L → F in CAA88658. Ref.1
	Sequence conflict	335	1	L → F in AAA65197. Ref.2
	Sequence conflict	509	1	N → K in CAA88658. Ref.1
	Sequence conflict	509	1	N → K in AAA65197. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q922K9-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: B79A0BC07B9EC5F8	FASTA	512	58,844
	10 20 30 40 50 60 MGSVCVRLWA YLQPFLPCWS QEADKSVVIE NPGAFCPPEA PRSQEPERSH GQYFVALFDY 70 80 90 100 110 120 QARTAEDLSF RAGDKLQVLD TSHEGWWLAR HLEKKGTGLG QQLQGYIPSN YVAEDRSLQA 130 140 150 160 170 180 EPWFFGAIKR ADAEKQLLYS ENQTGAFLIR ESESQKGDFS LSVLDEGVVK HYRIRRLDEG 190 200 210 220 230 240 GFFLTRRKVF STLNEFVNYY TTTSDGLCVK LEKPCLKIQV PTPFDLSYKT ADQWEIDRNS 250 260 270 280 290 300 IQLLKRLGSG QFGEVWEGLW NNTTPVAVKT LKPGSMDPND FLREAQIMKS LRHPKLIQLY 310 320 330 340 350 360 AVCTLEDPIY IITELMRHGS LQEYLQNDGG SKIHLIQQVD MAAQVASGMA YLESQNYIHR 370 380 390 400 410 420 DLAARNVLVG EHNIYKVADF GLARVFKVDN EDIYESKHEI KLPVKWTAPE AIRTNKFSIK 430 440 450 460 470 480 SDVWSFGILL YEIITYGKMP YSGMTGAQVI QMLSQNYRLP QPSNCPQQFY SIMLECWNVE 490 500 510 PKQRPTFETL HWKLEDYFET DCSYSDTNNF IN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"iyk, a novel intracellular protein tyrosine kinase differentially expressed in the mouse mammary gland and intestine." Thuveson M., Albrecht D., Zuercher G., Andres A., Ziemiecki A. Biochem. Biophys. Res. Commun. 209:582-589(1995) [PubMed: 7733928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: BALB/c. Tissue: Mammary gland.
[2]	"Cloning of BSK, a murine FRK homologue with a specific pattern of tissue distribution." Oberg-Welsh C., Welsh M. Gene 152:239-242(1995) [PubMed: 7835707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Kidney, Mammary gland and Skin.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[5]	"Solution structure of the SH3 and SH2 domains of FYN-related kinase." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 50-220.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z48757 mRNA. Translation: CAA88658.1.
L36132 mRNA. Translation: AAA65197.1.
AK028855 mRNA. Translation: BAC26155.1.
AK052614 mRNA. Translation: BAC35063.1.
AK166379 mRNA. Translation: BAE38741.1.
BC007137 mRNA. Translation: AAH07137.1.

IPI

IPI00462777.

PIR

I49552.

RefSeq

NP_001153016.1.
NP_034367.2.

UniGene

Mm.332432

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2D8J	NMR	-	A	50-115	[»]
2DLY	NMR	-	A	113-220	[»]

SMR

Q922K9. Positions 49-115, 113-223.

ModBase

Search...

Protein-protein interaction databases

STRING

Q922K9.

PTM databases

PhosphoSite

Q922K9.

Proteomic databases

PRIDE

Q922K9.

Genome annotation databases

Ensembl

ENSMUST00000019913; ENSMUSP00000019913; ENSMUSG00000019779; Mus musculus. [Genome view]

GeneID

14302.

KEGG

mmu:14302.

UCSC

uc007euy.1. mouse.

Organism-specific databases

CTD

14302.

MGI

MGI:103265. Frk.

Phylogenomic databases

eggNOG

roNOG06372.

HOGENOM

HBG755340.

HOVERGEN

Q922K9.

InParanoid

Q922K9.

OMA

QNDAGSK.

OrthoDB

EOG9QRKQD.

PhylomeDB

Q922K9.

Enzyme and pathway databases

BRENDA

2.7.10.2. 244.

Gene expression databases

ArrayExpress

Q922K9.

Bgee

Q922K9.

CleanEx

MM_FRK.

Genevestigator

Q922K9.

GermOnline

ENSMUSG00000019779. Mus musculus.

Family and domain databases

InterPro

IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR020473. SH3_region.
IPR020702. Tyr_kinase_non-rcpt_FRK.
IPR020635. Tyr_Pkinase_cat_dom.
IPR020685. Tyr_prot_kinase.
IPR008266. Tyr_prot_kinase_AS.
[Graphical view]

Gene3D

G3DSA:3.30.505.10. SH2. 1 hit.

PANTHER

PTHR23256:SF146. Tyr_kinase_non-rcpt_FRK. 1 hit.
PTHR23256. Tyr_prot_kinase. 1 hit.

Pfam

PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]

PRINTS

PR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.

SMART

SM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]

PROSITE

PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

285723.

SOURCE

Search...

Entry information

Entry name

FRK_MOUSE

Accession

Primary (citable) accession number: Q922K9
Secondary accession number(s): Q61364, Q61745

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)