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Q922K9 (FRK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine-protein kinase FRK

EC=2.7.10.2
Alternative name(s):
Beta-cell Src-homology tyrosine kinase
Short name=BSK
FYN-related kinase
Intestine tyrosine kinase
Gene names
Name:Frk
Synonyms:Bsk, Iyk
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length512 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor By similarity.

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure

Interacts (via the SH3-domain) with PTEN. Interacts with RB1 By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Note: Predominantly found in the nucleus, with a small fraction found in the cell periphery By similarity.

Tissue specificity

Expressed in intestinal tract, fetal and adult islets of Langerhans, kidney, liver and lung. Ref.1 Ref.2

Disruption phenotype

Mice are viable and do not show any histological abnormalities in epithelial tissues or develop any pathological and/or metabolic disorders associated with the failure of epithelial organs. Ref.5

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

Contains 1 protein kinase domain.

Contains 1 SH2 domain.

Contains 1 SH3 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 512512Tyrosine-protein kinase FRK
PRO_0000260826

Regions

Domain42 – 11776SH3
Domain123 – 21593SH2
Domain241 – 498258Protein kinase
Nucleotide binding247 – 2559ATP By similarity

Sites

Active site3611Proton acceptor By similarity
Binding site2691ATP By similarity

Amino acid modifications

Modified residue3941Phosphotyrosine; by autocatalysis

Experimental info

Sequence conflict1541S → T in CAA88658. Ref.1
Sequence conflict2371D → H in CAA88658. Ref.1
Sequence conflict3351L → F in CAA88658. Ref.1
Sequence conflict3351L → F in AAA65197. Ref.2
Sequence conflict5091N → K in CAA88658. Ref.1
Sequence conflict5091N → K in AAA65197. Ref.2

Secondary structure

..................................... 512
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q922K9 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: B79A0BC07B9EC5F8

FASTA51258,844
        10         20         30         40         50         60 
MGSVCVRLWA YLQPFLPCWS QEADKSVVIE NPGAFCPPEA PRSQEPERSH GQYFVALFDY 

        70         80         90        100        110        120 
QARTAEDLSF RAGDKLQVLD TSHEGWWLAR HLEKKGTGLG QQLQGYIPSN YVAEDRSLQA 

       130        140        150        160        170        180 
EPWFFGAIKR ADAEKQLLYS ENQTGAFLIR ESESQKGDFS LSVLDEGVVK HYRIRRLDEG 

       190        200        210        220        230        240 
GFFLTRRKVF STLNEFVNYY TTTSDGLCVK LEKPCLKIQV PTPFDLSYKT ADQWEIDRNS 

       250        260        270        280        290        300 
IQLLKRLGSG QFGEVWEGLW NNTTPVAVKT LKPGSMDPND FLREAQIMKS LRHPKLIQLY 

       310        320        330        340        350        360 
AVCTLEDPIY IITELMRHGS LQEYLQNDGG SKIHLIQQVD MAAQVASGMA YLESQNYIHR 

       370        380        390        400        410        420 
DLAARNVLVG EHNIYKVADF GLARVFKVDN EDIYESKHEI KLPVKWTAPE AIRTNKFSIK 

       430        440        450        460        470        480 
SDVWSFGILL YEIITYGKMP YSGMTGAQVI QMLSQNYRLP QPSNCPQQFY SIMLECWNVE 

       490        500        510 
PKQRPTFETL HWKLEDYFET DCSYSDTNNF IN 

« Hide

References

« Hide 'large scale' references
[1]"iyk, a novel intracellular protein tyrosine kinase differentially expressed in the mouse mammary gland and intestine."
Thuveson M., Albrecht D., Zuercher G., Andres A., Ziemiecki A.
Biochem. Biophys. Res. Commun. 209:582-589(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Mammary gland.
[2]"Cloning of BSK, a murine FRK homologue with a specific pattern of tissue distribution."
Oberg-Welsh C., Welsh M.
Gene 152:239-242(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Kidney, Mammary gland and Skin.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[5]"Characterization of mice deficient in the Src family nonreceptor tyrosine kinase Frk/rak."
Chandrasekharan S., Qiu T.H., Alkharouf N., Brantley K., Mitchell J.B., Liu E.T.
Mol. Cell. Biol. 22:5235-5247(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[6]"The tyrosine kinase FRK/RAK participates in cytokine-induced islet cell cytotoxicity."
Welsh M., Welsh C., Ekman M., Dixelius J., Hagerkvist R., Anneren C., Akerblom B., Mahboobi S., Chandrasekharan S., Liu E.T.
Biochem. J. 382:261-268(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: AUTOPHOSPHORYLATION.
[7]"Solution structure of the SH3 and SH2 domains of FYN-related kinase."
RIKEN structural genomics initiative (RSGI)
Submitted (OCT-2006) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 50-220.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z48757 mRNA. Translation: CAA88658.1.
L36132 mRNA. Translation: AAA65197.1.
AK028855 mRNA. Translation: BAC26155.1.
AK052614 mRNA. Translation: BAC35063.1.
AK166379 mRNA. Translation: BAE38741.1.
BC007137 mRNA. Translation: AAH07137.1.
PIRI49552.
RefSeqNP_001153016.1. NM_001159544.1.
NP_034367.2. NM_010237.3.
XP_006512597.1. XM_006512534.1.
XP_006512598.1. XM_006512535.1.
XP_006512599.1. XM_006512536.1.
UniGeneMm.332432.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2D8JNMR-A51-115[»]
2DLYNMR-A113-220[»]
ProteinModelPortalQ922K9.
SMRQ922K9. Positions 49-510.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000019913.

PTM databases

PhosphoSiteQ922K9.

Proteomic databases

PaxDbQ922K9.
PRIDEQ922K9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000019913; ENSMUSP00000019913; ENSMUSG00000019779.
ENSMUST00000170771; ENSMUSP00000130289; ENSMUSG00000019779.
GeneID14302.
KEGGmmu:14302.
UCSCuc007euy.2. mouse.

Organism-specific databases

CTD2444.
MGIMGI:103265. Frk.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00640000091347.
HOGENOMHOG000233858.
HOVERGENHBG008761.
InParanoidQ922K9.
KOK08892.
OMAVSHYTKT.
OrthoDBEOG7GTT2V.
PhylomeDBQ922K9.
TreeFamTF351634.

Gene expression databases

BgeeQ922K9.
CleanExMM_FRK.
GenevestigatorQ922K9.

Family and domain databases

Gene3D3.30.505.10. 1 hit.
InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ922K9.
NextBio285723.
PROQ922K9.
SOURCESearch...

Entry information

Entry nameFRK_MOUSE
AccessionPrimary (citable) accession number: Q922K9
Secondary accession number(s): Q61364, Q61745
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot