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Protein

Probable 28S rRNA (cytosine-C(5))-methyltransferase

Gene

Nop2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of a cytosine in 28S rRNA. May play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation.By similarity

Catalytic activityi

S-adenosyl-L-methionine + cytosine in 28S rRNA = S-adenosyl-L-homocysteine + C(5)-methylcytosine in 28S rRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei401 – 4011S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei428 – 4281S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei445 – 4451S-adenosyl-L-methioninePROSITE-ProRule annotation
Active sitei502 – 5021NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 28S rRNA (cytosine-C(5))-methyltransferase (EC:2.1.1.-)
Alternative name(s):
Nucleolar protein 1
Nucleolar protein 2 homolog
Proliferating-cell nucleolar antigen p120
Proliferation-associated nucleolar protein p120
Gene namesi
Name:Nop2
Synonyms:Nol1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:107891. Nop2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 793793Probable 28S rRNA (cytosine-C(5))-methyltransferasePRO_0000279585Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei59 – 591PhosphoserineBy similarity
Modified residuei68 – 681PhosphoserineBy similarity
Modified residuei86 – 861Citrulline1 Publication
Modified residuei148 – 1481Citrulline1 Publication
Modified residuei169 – 1691PhosphothreonineCombined sources
Modified residuei179 – 1791PhosphoserineCombined sources
Cross-linki601 – 601Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei634 – 6341N6-acetyllysineBy similarity
Modified residuei649 – 6491PhosphoserineBy similarity
Modified residuei713 – 7131PhosphoserineBy similarity
Modified residuei767 – 7671PhosphoserineBy similarity
Modified residuei782 – 7821PhosphoserineBy similarity
Modified residuei793 – 7931PhosphoserineBy similarity

Post-translational modificationi

Citrullinated by PADI4.1 Publication

Keywords - PTMi

Acetylation, Citrullination, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ922K7.
MaxQBiQ922K7.
PaxDbiQ922K7.
PeptideAtlasiQ922K7.
PRIDEiQ922K7.

PTM databases

iPTMnetiQ922K7.
PhosphoSiteiQ922K7.

Interactioni

Subunit structurei

Interaction with MCRS1. Interacts with WDR46.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047123.

Structurei

3D structure databases

ProteinModelPortaliQ922K7.
SMRiQ922K7. Positions 265-579.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni377 – 3837S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1122. Eukaryota.
COG0144. LUCA.
HOGENOMiHOG000224258.
HOVERGENiHBG082043.
InParanoidiQ922K7.
PhylomeDBiQ922K7.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR031341. Methyltr_RsmF_N.
IPR001678. MeTrfase_RsmB/NOP2.
IPR011023. Nop2p.
IPR012586. P120R_rpt.
IPR023267. RCMT.
IPR023273. RCMT_NOP2.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF17125. Methyltr_RsmF_N. 1 hit.
PF08062. P120R. 2 hits.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
PR02012. RCMTNOP2.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00446. nop2p. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q922K7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRKLDPTKK EKRGPGRKAR KQKGAETELV RFLPAAGDEN SKRLSSRARK
60 70 80 90 100
RAAKRRAGSV DVPKPNKSPG IKTLPGELSK GAVQARGKKR PAPIQNSDGD
110 120 130 140 150
EEEDSGEDDV VTQGDLWGSE DSDEDMVDDY GAASNSEDEE EKLLPIERAA
160 170 180 190 200
LKQKAQDATA GVLWNEEDTD EDEDDDGVSP ESHPRKDDKA EGDLQINVED
210 220 230 240 250
EEAFVLPPAG ETDQDGQAPD LQRVHKRIQD IVGVLRDFGA QREEGRSRAE
260 270 280 290 300
YLSRLQKDLA TYYSYGDFLL SKLMELFPLS ELIEFLEANE VPRPITLRTN
310 320 330 340 350
TLKTRRRDLA QLLINRGVNL DPLGKWSKSG LVVYDSSVPI GATPEYLAGH
360 370 380 390 400
YMLQGASSML PVMALAPQEH ERILDMCCAP GGKTSYIAQL MKNTGVILAN
410 420 430 440 450
DANADRLKSV VGNLHRLGVT NTIISHYDGR QFPKVVGGFD RVLLDAPCSG
460 470 480 490 500
TGVISKDPAV KTNKDEKDIQ RCAHLQKELL LSAIDSVNAA SKTGGYLVYC
510 520 530 540 550
TCSITVEENE WVVDYALKKR NVRLVPTGLD FGQEGFTRFQ ARRFHPTLRS
560 570 580 590 600
TRRFYPHTHN MDGFFIAKFK KFSNSIPQPH AGNSAAATPT EPDLKDQVTP
610 620 630 640 650
KSENGSQPTK KARGAVKAKQ QLLRQPHSKK PFQKLNGIAK GPGLSTEPSV
660 670 680 690 700
PDAQVSTRPS QSAGNADVNS KRKRSEKLKQ RGPKWKPSKE AAVPKPSAPS
710 720 730 740 750
RVEDSGTPVP TPSEIRAAPR PKDCAPSLGK AKKKQKGKQQ LAQQPANGAA
760 770 780 790
PLKEDAVSKG PSAPFVSPHS STRPPPAKRR KSMTKGNSQP LLS
Length:793
Mass (Da):86,752
Last modified:December 1, 2001 - v1
Checksum:iCB3B52A01A2E753F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti160 – 1601A → T in BAE32311 (PubMed:16141072).Curated
Sequence conflicti312 – 3121L → A in BAE32311 (PubMed:16141072).Curated
Sequence conflicti708 – 7081P → PP in BAE32311 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC007151 mRNA. Translation: AAH07151.1.
AK154006 mRNA. Translation: BAE32311.1.
PIRiA48998.
UniGeneiMm.29203.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC007151 mRNA. Translation: AAH07151.1.
AK154006 mRNA. Translation: BAE32311.1.
PIRiA48998.
UniGeneiMm.29203.

3D structure databases

ProteinModelPortaliQ922K7.
SMRiQ922K7. Positions 265-579.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047123.

PTM databases

iPTMnetiQ922K7.
PhosphoSiteiQ922K7.

Proteomic databases

EPDiQ922K7.
MaxQBiQ922K7.
PaxDbiQ922K7.
PeptideAtlasiQ922K7.
PRIDEiQ922K7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:107891. Nop2.

Phylogenomic databases

eggNOGiKOG1122. Eukaryota.
COG0144. LUCA.
HOGENOMiHOG000224258.
HOVERGENiHBG082043.
InParanoidiQ922K7.
PhylomeDBiQ922K7.

Miscellaneous databases

PROiQ922K7.
SOURCEiSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018314. Fmu/NOL1/Nop2p_CS.
IPR031341. Methyltr_RsmF_N.
IPR001678. MeTrfase_RsmB/NOP2.
IPR011023. Nop2p.
IPR012586. P120R_rpt.
IPR023267. RCMT.
IPR023273. RCMT_NOP2.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF17125. Methyltr_RsmF_N. 1 hit.
PF08062. P120R. 2 hits.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
PR02012. RCMTNOP2.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00446. nop2p. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A region of antisense RNA from human p120 cDNA with high homology to mouse p120 cDNA inhibits NIH 3T3 proliferation."
    Valdez B.C., Perlaky L., Saijo Y., Henning D., Zhu C., Busch R.K., Zhang W.W., Busch H.
    Cancer Res. 52:5681-5686(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary tumor.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-731.
    Strain: NOD.
    Tissue: Thymus.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-169 AND SER-179, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney, Lung, Pancreas, Spleen and Testis.
  5. Cited for: CITRULLINATION AT ARG-86 AND ARG-148.

Entry informationi

Entry nameiNOP2_MOUSE
AccessioniPrimary (citable) accession number: Q922K7
Secondary accession number(s): Q3U4W9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: December 1, 2001
Last modified: July 6, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.