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Q922J3

- CLIP1_MOUSE

UniProt

Q922J3 - CLIP1_MOUSE

Protein

CAP-Gly domain-containing linker protein 1

Gene

Clip1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1370 – 138718CCHC-typeAdd
    BLAST

    GO - Molecular functioni

    1. microtubule binding Source: MGI
    2. microtubule plus-end binding Source: MGI
    3. nucleic acid binding Source: InterPro
    4. protein binding Source: MGI
    5. tubulin binding Source: UniProtKB
    6. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. microtubule bundle formation Source: UniProtKB
    2. positive regulation of microtubule polymerization Source: UniProtKB
    3. transport Source: UniProtKB-KW

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_207679. Separation of Sister Chromatids.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CAP-Gly domain-containing linker protein 1
    Alternative name(s):
    Restin
    Gene namesi
    Name:Clip1
    Synonyms:Kiaa4046, Rsn
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1928401. Clip1.

    Subcellular locationi

    Cytoplasm By similarity. Cytoplasmcytoskeleton By similarity. Cytoplasmic vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Cell projectionruffle By similarity
    Note: Associated with the cytoskeleton. Detected at the plus ends of microtubules in the cytosol, and close to plasma membrane ruffles. Associates with the membranes of intermediate macropinocytic vesicles By similarity.By similarity

    GO - Cellular componenti

    1. centrosome Source: Ensembl
    2. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    3. macropinosome Source: Ensembl
    4. microtubule Source: UniProtKB
    5. microtubule cytoskeleton Source: UniProtKB
    6. microtubule plus-end Source: Ensembl
    7. ruffle Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13911391CAP-Gly domain-containing linker protein 1PRO_0000240672Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei48 – 481PhosphoserineBy similarity
    Modified residuei146 – 1461PhosphoserineBy similarity
    Modified residuei181 – 1811PhosphothreonineBy similarity
    Modified residuei194 – 1941PhosphoserineBy similarity
    Modified residuei196 – 1961PhosphoserineBy similarity
    Modified residuei199 – 1991Phosphoserine1 Publication
    Modified residuei203 – 2031Phosphoserine2 Publications
    Modified residuei1317 – 13171PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated. Phosphorylation by MTOR may positively regulate CLIP1 association with microtubules By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ922J3.
    PaxDbiQ922J3.
    PRIDEiQ922J3.

    PTM databases

    PhosphoSiteiQ922J3.

    Expressioni

    Gene expression databases

    ArrayExpressiQ922J3.
    BgeeiQ922J3.
    CleanExiMM_CLIP1.
    GenevestigatoriQ922J3.

    Interactioni

    Subunit structurei

    Interacts with MTOR; phosphorylates and regulates CLIP1. Interacts (via CAP-Gly domains) with tubulin. Interacts with SLAIN2. Interacts with MAPRE1 and MAPRE3. Interacts (via zinc finger) with DCTN1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi207974. 3 interactions.
    IntActiQ922J3. 4 interactions.
    MINTiMINT-4122817.
    STRINGi10090.ENSMUSP00000031382.

    Structurei

    Secondary structure

    1
    1391
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi63 – 664
    Beta strandi71 – 799
    Beta strandi81 – 833
    Beta strandi85 – 9511
    Beta strandi97 – 1037
    Beta strandi116 – 1194
    Helixi121 – 1233

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CP7NMR-A58-128[»]
    ProteinModelPortaliQ922J3.
    SMRiQ922J3. Positions 4-130, 180-339.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ922J3.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini78 – 12043CAP-Gly 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini231 – 27343CAP-Gly 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni97 – 1015Important for tubulin bindingBy similarity

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili349 – 1306958Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi305 – 33026Ser-richAdd
    BLAST

    Domaini

    Intramolecular interaction between the zinc finger domain and the CAP-Gly domains may inhibit interaction with tubulin.By similarity

    Sequence similaritiesi

    Contains 2 CAP-Gly domains.PROSITE-ProRule annotation
    Contains 1 CCHC-type zinc finger.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1370 – 138718CCHC-typeAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5244.
    GeneTreeiENSGT00740000115338.
    HOGENOMiHOG000092755.
    HOVERGENiHBG007123.
    KOiK10421.
    OrthoDBiEOG7992PN.
    PhylomeDBiQ922J3.
    TreeFamiTF326096.

    Family and domain databases

    Gene3Di2.30.30.190. 2 hits.
    InterProiIPR000938. CAP-Gly_domain.
    IPR001878. Znf_CCHC.
    [Graphical view]
    PfamiPF01302. CAP_GLY. 2 hits.
    [Graphical view]
    SMARTiSM01052. CAP_GLY. 2 hits.
    SM00343. ZnF_C2HC. 1 hit.
    [Graphical view]
    SUPFAMiSSF74924. SSF74924. 2 hits.
    PROSITEiPS00845. CAP_GLY_1. 2 hits.
    PS50245. CAP_GLY_2. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q922J3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSMLKPSGLK APTKILKPGS TALKTPAAAA APVEKTIPSE KASGPPSSET     50
    QEEFVDDFRV GERVWVNGNK PGFIQFLGET QFAPGQWAGI VLDEPIGKND 100
    GSVAGVRYFQ CEPLKGIFTR PSKLTRKVQA EDEANGLQAA PGRTASPLST 150
    AAATMVSSSP ATPSNIPHKP SQSTAKEPSA TPQISNLTKT ASESISNLSE 200
    AGSVKKGERE LKVGDRVLVG GTKAGVVRFL GETDFAKGEW CGVELDEPLG 250
    KNDGAVAGTR YFQCQPKYGL FAPVHKVTKI GFPSTTPAKA KAAAVRRVMA 300
    ATPASLKRSP SASSLSSMSS VASSVSSKPS RTGLLTETSS RYARKISGTT 350
    ALQEALKEKQ QHIEQLLAER DLERAEVAKA TSHVGEIEQE LALARDGHDQ 400
    HVLELEAKMD QLRTMVEAAD REKVELLNQL EEEKRKVEDL QFRVEEESIT 450
    KGDLEVATVS EKSRIMELEK DLALRAQEVA ELRRRLESSK PPGDVDMSLS 500
    LLQEISALQE KLEAIHTDHQ GEMTSLKEHF GAREEAFQKE IKALHTATEK 550
    LSKENESLRS KLDHANKENS DVIALWKSKL ETAIASHQQA MEELKVSFSK 600
    GIGTDSAEFA ELKTQIERLR LDYQHEIESL QSKQDSERSA HAKEMETMQA 650
    KLMKIIKEKE DSLEAVKARL DSAEDQHLVE MEDTLNKLQE AEIKVKELEV 700
    LQAKYTEQSE VIGNFTSQLS AVKEKLLDLD ALRKANSEGK LELETLRQQL 750
    EGAEKQIKNL ETERNAESSK ANSITKELQE KELVLTGLQD SLNQVNQVKE 800
    TLEKELQTLK EKFASTSEEA VSAQTRMQDT VNKLHQKEEQ FNVLSSELEK 850
    LRENLTDMEA KFKEKDDRED QLVKAKEKLE NDIAEIMKMS GDNSSQLTKM 900
    NDELRLKERS VEELQLKLTK ANENASFLQK SIGEVTLKAE QSQQQAARKH 950
    EEEKKELEEK LLELEKKMET SYNQCQDLKA KYEKASSETK TKHEEILQNL 1000
    QKMLADTEDK LKAAQEANRD LMQDMEELKT QADKAKAAQT AEDAMQIMEQ 1050
    MTKEKTETLA SLEDTKQTNA RLQNELDTLK ENNLKTVEEL NKSKELLSVE 1100
    NQKMEEFKKE IETLKQAAAQ KSQQLSALQE ENVKLAEELG RTRDEVTSHQ 1150
    KLEEERSVLN NQLLEMKKRE SEFRKDADEE KASLQKSISL TSALLTEKDA 1200
    ELEKLRNEVT VLRGENATAK SLHSVVQTLE SDKVKLELKV KNLELQLKEN 1250
    KRQLSSSSGN TDAQAEEDER AQESQIDFLN SVIVDLQRKN QDLKMKVEMM 1300
    SEAALNGNGE DLNSYDSDDQ EKQSKKKPRL FCDICDCFDL HDTEDCPTQA 1350
    QMSEDPPHST HHGSRSEERP YCEICEMFGH WATNCNDDET F 1391
    Length:1,391
    Mass (Da):155,814
    Last modified:December 1, 2001 - v1
    Checksum:i061BED1FB3D4068D
    GO
    Isoform 2 (identifier: Q922J3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         695-770: Missing.

    Show »
    Length:1,315
    Mass (Da):147,242
    Checksum:i449A0909164DFB02
    GO

    Sequence cautioni

    The sequence BAD90357.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti47 – 471S → F in BAD90357. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei695 – 77076Missing in isoform 2. 1 PublicationVSP_019425Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK220172 mRNA. Translation: BAD90357.1. Different initiation.
    BC007191 mRNA. Translation: AAH07191.1.
    CCDSiCCDS19667.1. [Q922J3-1]
    CCDS71663.1. [Q922J3-2]
    RefSeqiNP_001278158.1. NM_001291229.1. [Q922J3-2]
    NP_062739.2. NM_019765.5. [Q922J3-1]
    UniGeneiMm.241109.
    Mm.441802.

    Genome annotation databases

    EnsembliENSMUST00000111564; ENSMUSP00000107190; ENSMUSG00000049550. [Q922J3-2]
    ENSMUST00000111566; ENSMUSP00000107192; ENSMUSG00000049550. [Q922J3-1]
    GeneIDi56430.
    KEGGimmu:56430.
    UCSCiuc008zoa.1. mouse. [Q922J3-1]
    uc008zob.1. mouse. [Q922J3-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK220172 mRNA. Translation: BAD90357.1 . Different initiation.
    BC007191 mRNA. Translation: AAH07191.1 .
    CCDSi CCDS19667.1. [Q922J3-1 ]
    CCDS71663.1. [Q922J3-2 ]
    RefSeqi NP_001278158.1. NM_001291229.1. [Q922J3-2 ]
    NP_062739.2. NM_019765.5. [Q922J3-1 ]
    UniGenei Mm.241109.
    Mm.441802.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CP7 NMR - A 58-128 [» ]
    ProteinModelPortali Q922J3.
    SMRi Q922J3. Positions 4-130, 180-339.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 207974. 3 interactions.
    IntActi Q922J3. 4 interactions.
    MINTi MINT-4122817.
    STRINGi 10090.ENSMUSP00000031382.

    PTM databases

    PhosphoSitei Q922J3.

    Proteomic databases

    MaxQBi Q922J3.
    PaxDbi Q922J3.
    PRIDEi Q922J3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000111564 ; ENSMUSP00000107190 ; ENSMUSG00000049550 . [Q922J3-2 ]
    ENSMUST00000111566 ; ENSMUSP00000107192 ; ENSMUSG00000049550 . [Q922J3-1 ]
    GeneIDi 56430.
    KEGGi mmu:56430.
    UCSCi uc008zoa.1. mouse. [Q922J3-1 ]
    uc008zob.1. mouse. [Q922J3-2 ]

    Organism-specific databases

    CTDi 6249.
    MGIi MGI:1928401. Clip1.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG5244.
    GeneTreei ENSGT00740000115338.
    HOGENOMi HOG000092755.
    HOVERGENi HBG007123.
    KOi K10421.
    OrthoDBi EOG7992PN.
    PhylomeDBi Q922J3.
    TreeFami TF326096.

    Enzyme and pathway databases

    Reactomei REACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_207679. Separation of Sister Chromatids.

    Miscellaneous databases

    EvolutionaryTracei Q922J3.
    NextBioi 312602.
    PROi Q922J3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q922J3.
    Bgeei Q922J3.
    CleanExi MM_CLIP1.
    Genevestigatori Q922J3.

    Family and domain databases

    Gene3Di 2.30.30.190. 2 hits.
    InterProi IPR000938. CAP-Gly_domain.
    IPR001878. Znf_CCHC.
    [Graphical view ]
    Pfami PF01302. CAP_GLY. 2 hits.
    [Graphical view ]
    SMARTi SM01052. CAP_GLY. 2 hits.
    SM00343. ZnF_C2HC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF74924. SSF74924. 2 hits.
    PROSITEi PS00845. CAP_GLY_1. 2 hits.
    PS50245. CAP_GLY_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
      Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Embryonic tail.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Mammary tumor.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic brain.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199 AND SER-203, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    5. "ACF7 regulates cytoskeletal-focal adhesion dynamics and migration and has ATPase activity."
      Wu X., Kodama A., Fuchs E.
      Cell 135:137-148(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    6. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    7. "Solution structure of the 1st CAP-Gly domain in mouse CLIP-170/Restin."
      RIKEN structural genomics initiative (RSGI)
      Submitted (NOV-2005) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 58-128.

    Entry informationi

    Entry nameiCLIP1_MOUSE
    AccessioniPrimary (citable) accession number: Q922J3
    Secondary accession number(s): Q571L7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 27, 2006
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3