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Q922J3

- CLIP1_MOUSE

UniProt

Q922J3 - CLIP1_MOUSE

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Protein
CAP-Gly domain-containing linker protein 1
Gene
Clip1, Kiaa4046, Rsn
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1370 – 138718CCHC-type
Add
BLAST

GO - Molecular functioni

  1. microtubule binding Source: MGI
  2. microtubule plus-end binding Source: MGI
  3. nucleic acid binding Source: InterPro
  4. protein binding Source: MGI
  5. tubulin binding Source: UniProtKB
  6. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. microtubule bundle formation Source: UniProtKB
  2. positive regulation of microtubule polymerization Source: UniProtKB
  3. transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_198961. Resolution of Sister Chromatid Cohesion.
REACT_207679. Separation of Sister Chromatids.

Names & Taxonomyi

Protein namesi
Recommended name:
CAP-Gly domain-containing linker protein 1
Alternative name(s):
Restin
Gene namesi
Name:Clip1
Synonyms:Kiaa4046, Rsn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1928401. Clip1.

Subcellular locationi

Cytoplasm By similarity. Cytoplasmcytoskeleton By similarity. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cell projectionruffle By similarity
Note: Associated with the cytoskeleton. Detected at the plus ends of microtubules in the cytosol, and close to plasma membrane ruffles. Associates with the membranes of intermediate macropinocytic vesicles By similarity.1 Publication

GO - Cellular componenti

  1. centrosome Source: Ensembl
  2. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  3. macropinosome Source: Ensembl
  4. microtubule Source: UniProtKB
  5. microtubule cytoskeleton Source: UniProtKB
  6. microtubule plus-end Source: Ensembl
  7. ruffle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13911391CAP-Gly domain-containing linker protein 1
PRO_0000240672Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei48 – 481Phosphoserine By similarity
Modified residuei146 – 1461Phosphoserine By similarity
Modified residuei181 – 1811Phosphothreonine By similarity
Modified residuei194 – 1941Phosphoserine By similarity
Modified residuei196 – 1961Phosphoserine By similarity
Modified residuei199 – 1991Phosphoserine1 Publication
Modified residuei203 – 2031Phosphoserine2 Publications
Modified residuei1317 – 13171Phosphoserine By similarity

Post-translational modificationi

Phosphorylated. Phosphorylation by MTOR may positively regulate CLIP1 association with microtubules By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ922J3.
PaxDbiQ922J3.
PRIDEiQ922J3.

PTM databases

PhosphoSiteiQ922J3.

Expressioni

Gene expression databases

ArrayExpressiQ922J3.
BgeeiQ922J3.
CleanExiMM_CLIP1.
GenevestigatoriQ922J3.

Interactioni

Subunit structurei

Interacts with MTOR; phosphorylates and regulates CLIP1. Interacts (via CAP-Gly domains) with tubulin. Interacts with SLAIN2. Interacts with MAPRE1 and MAPRE3. Interacts (via zinc finger) with DCTN1 By similarity.

Protein-protein interaction databases

BioGridi207974. 3 interactions.
IntActiQ922J3. 4 interactions.
MINTiMINT-4122817.
STRINGi10090.ENSMUSP00000031382.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi63 – 664
Beta strandi71 – 799
Beta strandi81 – 833
Beta strandi85 – 9511
Beta strandi97 – 1037
Beta strandi116 – 1194
Helixi121 – 1233

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CP7NMR-A58-128[»]
ProteinModelPortaliQ922J3.
SMRiQ922J3. Positions 4-130, 180-339.

Miscellaneous databases

EvolutionaryTraceiQ922J3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini78 – 12043CAP-Gly 1
Add
BLAST
Domaini231 – 27343CAP-Gly 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni97 – 1015Important for tubulin binding By similarity

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili349 – 1306958 Reviewed prediction
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi305 – 33026Ser-rich
Add
BLAST

Domaini

Intramolecular interaction between the zinc finger domain and the CAP-Gly domains may inhibit interaction with tubulin By similarity.

Sequence similaritiesi

Contains 2 CAP-Gly domains.

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5244.
GeneTreeiENSGT00740000115338.
HOGENOMiHOG000092755.
HOVERGENiHBG007123.
KOiK10421.
OrthoDBiEOG7992PN.
PhylomeDBiQ922J3.
TreeFamiTF326096.

Family and domain databases

Gene3Di2.30.30.190. 2 hits.
InterProiIPR000938. CAP-Gly_domain.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF01302. CAP_GLY. 2 hits.
[Graphical view]
SMARTiSM01052. CAP_GLY. 2 hits.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMiSSF74924. SSF74924. 2 hits.
PROSITEiPS00845. CAP_GLY_1. 2 hits.
PS50245. CAP_GLY_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q922J3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSMLKPSGLK APTKILKPGS TALKTPAAAA APVEKTIPSE KASGPPSSET     50
QEEFVDDFRV GERVWVNGNK PGFIQFLGET QFAPGQWAGI VLDEPIGKND 100
GSVAGVRYFQ CEPLKGIFTR PSKLTRKVQA EDEANGLQAA PGRTASPLST 150
AAATMVSSSP ATPSNIPHKP SQSTAKEPSA TPQISNLTKT ASESISNLSE 200
AGSVKKGERE LKVGDRVLVG GTKAGVVRFL GETDFAKGEW CGVELDEPLG 250
KNDGAVAGTR YFQCQPKYGL FAPVHKVTKI GFPSTTPAKA KAAAVRRVMA 300
ATPASLKRSP SASSLSSMSS VASSVSSKPS RTGLLTETSS RYARKISGTT 350
ALQEALKEKQ QHIEQLLAER DLERAEVAKA TSHVGEIEQE LALARDGHDQ 400
HVLELEAKMD QLRTMVEAAD REKVELLNQL EEEKRKVEDL QFRVEEESIT 450
KGDLEVATVS EKSRIMELEK DLALRAQEVA ELRRRLESSK PPGDVDMSLS 500
LLQEISALQE KLEAIHTDHQ GEMTSLKEHF GAREEAFQKE IKALHTATEK 550
LSKENESLRS KLDHANKENS DVIALWKSKL ETAIASHQQA MEELKVSFSK 600
GIGTDSAEFA ELKTQIERLR LDYQHEIESL QSKQDSERSA HAKEMETMQA 650
KLMKIIKEKE DSLEAVKARL DSAEDQHLVE MEDTLNKLQE AEIKVKELEV 700
LQAKYTEQSE VIGNFTSQLS AVKEKLLDLD ALRKANSEGK LELETLRQQL 750
EGAEKQIKNL ETERNAESSK ANSITKELQE KELVLTGLQD SLNQVNQVKE 800
TLEKELQTLK EKFASTSEEA VSAQTRMQDT VNKLHQKEEQ FNVLSSELEK 850
LRENLTDMEA KFKEKDDRED QLVKAKEKLE NDIAEIMKMS GDNSSQLTKM 900
NDELRLKERS VEELQLKLTK ANENASFLQK SIGEVTLKAE QSQQQAARKH 950
EEEKKELEEK LLELEKKMET SYNQCQDLKA KYEKASSETK TKHEEILQNL 1000
QKMLADTEDK LKAAQEANRD LMQDMEELKT QADKAKAAQT AEDAMQIMEQ 1050
MTKEKTETLA SLEDTKQTNA RLQNELDTLK ENNLKTVEEL NKSKELLSVE 1100
NQKMEEFKKE IETLKQAAAQ KSQQLSALQE ENVKLAEELG RTRDEVTSHQ 1150
KLEEERSVLN NQLLEMKKRE SEFRKDADEE KASLQKSISL TSALLTEKDA 1200
ELEKLRNEVT VLRGENATAK SLHSVVQTLE SDKVKLELKV KNLELQLKEN 1250
KRQLSSSSGN TDAQAEEDER AQESQIDFLN SVIVDLQRKN QDLKMKVEMM 1300
SEAALNGNGE DLNSYDSDDQ EKQSKKKPRL FCDICDCFDL HDTEDCPTQA 1350
QMSEDPPHST HHGSRSEERP YCEICEMFGH WATNCNDDET F 1391
Length:1,391
Mass (Da):155,814
Last modified:December 1, 2001 - v1
Checksum:i061BED1FB3D4068D
GO
Isoform 2 (identifier: Q922J3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     695-770: Missing.

Show »
Length:1,315
Mass (Da):147,242
Checksum:i449A0909164DFB02
GO

Sequence cautioni

The sequence BAD90357.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei695 – 77076Missing in isoform 2.
VSP_019425Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti47 – 471S → F in BAD90357. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK220172 mRNA. Translation: BAD90357.1. Different initiation.
BC007191 mRNA. Translation: AAH07191.1.
CCDSiCCDS19667.1. [Q922J3-1]
CCDS71663.1. [Q922J3-2]
RefSeqiNP_001278158.1. NM_001291229.1. [Q922J3-2]
NP_062739.2. NM_019765.5. [Q922J3-1]
UniGeneiMm.241109.
Mm.441802.

Genome annotation databases

EnsembliENSMUST00000111564; ENSMUSP00000107190; ENSMUSG00000049550. [Q922J3-2]
ENSMUST00000111566; ENSMUSP00000107192; ENSMUSG00000049550. [Q922J3-1]
GeneIDi56430.
KEGGimmu:56430.
UCSCiuc008zoa.1. mouse. [Q922J3-1]
uc008zob.1. mouse. [Q922J3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK220172 mRNA. Translation: BAD90357.1 . Different initiation.
BC007191 mRNA. Translation: AAH07191.1 .
CCDSi CCDS19667.1. [Q922J3-1 ]
CCDS71663.1. [Q922J3-2 ]
RefSeqi NP_001278158.1. NM_001291229.1. [Q922J3-2 ]
NP_062739.2. NM_019765.5. [Q922J3-1 ]
UniGenei Mm.241109.
Mm.441802.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2CP7 NMR - A 58-128 [» ]
ProteinModelPortali Q922J3.
SMRi Q922J3. Positions 4-130, 180-339.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 207974. 3 interactions.
IntActi Q922J3. 4 interactions.
MINTi MINT-4122817.
STRINGi 10090.ENSMUSP00000031382.

PTM databases

PhosphoSitei Q922J3.

Proteomic databases

MaxQBi Q922J3.
PaxDbi Q922J3.
PRIDEi Q922J3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000111564 ; ENSMUSP00000107190 ; ENSMUSG00000049550 . [Q922J3-2 ]
ENSMUST00000111566 ; ENSMUSP00000107192 ; ENSMUSG00000049550 . [Q922J3-1 ]
GeneIDi 56430.
KEGGi mmu:56430.
UCSCi uc008zoa.1. mouse. [Q922J3-1 ]
uc008zob.1. mouse. [Q922J3-2 ]

Organism-specific databases

CTDi 6249.
MGIi MGI:1928401. Clip1.
Rougei Search...

Phylogenomic databases

eggNOGi COG5244.
GeneTreei ENSGT00740000115338.
HOGENOMi HOG000092755.
HOVERGENi HBG007123.
KOi K10421.
OrthoDBi EOG7992PN.
PhylomeDBi Q922J3.
TreeFami TF326096.

Enzyme and pathway databases

Reactomei REACT_198961. Resolution of Sister Chromatid Cohesion.
REACT_207679. Separation of Sister Chromatids.

Miscellaneous databases

EvolutionaryTracei Q922J3.
NextBioi 312602.
PROi Q922J3.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q922J3.
Bgeei Q922J3.
CleanExi MM_CLIP1.
Genevestigatori Q922J3.

Family and domain databases

Gene3Di 2.30.30.190. 2 hits.
InterProi IPR000938. CAP-Gly_domain.
IPR001878. Znf_CCHC.
[Graphical view ]
Pfami PF01302. CAP_GLY. 2 hits.
[Graphical view ]
SMARTi SM01052. CAP_GLY. 2 hits.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view ]
SUPFAMi SSF74924. SSF74924. 2 hits.
PROSITEi PS00845. CAP_GLY_1. 2 hits.
PS50245. CAP_GLY_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Embryonic tail.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199 AND SER-203, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "ACF7 regulates cytoskeletal-focal adhesion dynamics and migration and has ATPase activity."
    Wu X., Kodama A., Fuchs E.
    Cell 135:137-148(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  7. "Solution structure of the 1st CAP-Gly domain in mouse CLIP-170/Restin."
    RIKEN structural genomics initiative (RSGI)
    Submitted (NOV-2005) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 58-128.

Entry informationi

Entry nameiCLIP1_MOUSE
AccessioniPrimary (citable) accession number: Q922J3
Secondary accession number(s): Q571L7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: December 1, 2001
Last modified: September 3, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi