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Q922H1 (ANM3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein arginine N-methyltransferase 3

EC=2.1.1.-
Alternative name(s):
Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3
Gene names
Name:Prmt3
Synonyms:Hrmt1l3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length532 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins By similarity.

Enzyme regulation

Inhibited by N-ethylmaleimide and high concentrations of zinc chloride By similarity.

Subunit structure

Monomer or homodimer By similarity. Interacts with EPB41L3; this inhibits methylation of target proteins. Interacts with the 40S ribosomal protein RPS2 By similarity.

Subcellular location

Cytoplasm By similarity.

Domain

The zinc-finger is responsible for substrate specificity By similarity.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.

Contains 1 C2H2-type zinc finger.

Contains 1 SAM-dependent MTase PRMT-type domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q922H1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform 2 (identifier: Q922H1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     417-420: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 532531Protein arginine N-methyltransferase 3
PRO_0000212327

Regions

Domain214 – 532319SAM-dependent MTase PRMT-type
Zinc finger46 – 6924C2H2-type

Sites

Active site3261 By similarity
Active site3351 By similarity
Binding site2271S-adenosyl-L-methionine By similarity
Binding site2361S-adenosyl-L-methionine By similarity
Binding site2601S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site2821S-adenosyl-L-methionine By similarity
Binding site3111S-adenosyl-L-methionine By similarity

Amino acid modifications

Modified residue21N-acetylcysteine By similarity
Modified residue221Phosphoserine By similarity
Modified residue241Phosphoserine By similarity

Natural variations

Alternative sequence417 – 4204Missing in isoform 2.
VSP_010498

Secondary structure

.................... 532
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 7, 2004. Version 2.
Checksum: 878ADDCCF4054F17

FASTA53259,902
        10         20         30         40         50         60 
MCSLAAGNGR GAELGPEPLE LSDSGDDAGW EDEDADTEPA HGRQHTPCLF CDRLFASAEE 

        70         80         90        100        110        120 
TFSHCKLEHQ FNIDSMVHKH GLEFYGYIKL INFIRLKNPT VEYMNSIYNP VPWEKDEYLK 

       130        140        150        160        170        180 
PVLEDDLLLQ FDVEDLYEPV STPFSYPNGL SESASVVEKL KHMEARALSA EAALARARED 

       190        200        210        220        230        240 
LQKMKQFAQD FVMNVDVRTC SSTTTIADLQ EDEDGVYFSS YGHYGIHEEM LKDKVRTESY 

       250        260        270        280        290        300 
RDFIYQNPHI FKDKVVLDVG CGTGILSMFA AKVGAKKVIA VDQSEILYQA MDIIRLNKLE 

       310        320        330        340        350        360 
DTIVLIKGKI EEVSLPVEKV DVIISEWMGY FLLFESMLDS VLYAKSKYLA KGGSVYPDIC 

       370        380        390        400        410        420 
TISLVAVSDV SKHADRIAFW DDVYGFNMSC MKKAVIPEAV VEVVDHKTLI SDPCDIKMDG 

       430        440        450        460        470        480 
KHIDCHTTSI SDLEFSSDFT LRTTKTAMCT AVAGYFDIYF EKNCHNRVVF STGPQSTKTH 

       490        500        510        520        530 
WKQTVFLLEK PFPVKAGEAL KGKITVHKNK KDPRSLIVTL TLNSSTQTYS LQ 

« Hide

Isoform 2 [UniParc].

Checksum: E8DF5B88AB6B92FF
Show »

FASTA52859,471

References

« Hide 'large scale' references
[1]"Genomic organization, chromosomal mapping, and expression analysis of the murine Prmt3 and Htatip2 genes."
Hauser L.J., Webb L.S., Dhar M.S., Mural R.M., Larimer F.W., Johnson D.K.
Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 2).
Strain: 129/SvJ.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Head and Thymus.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 114-532 (ISOFORM 2).
Strain: FVB/N.
Tissue: Brain and Mammary gland.
[4]"Solution structure of the C2H2 zinc finger domain of the protein arginine N-methyltransferase 3 from Mus musculus."
RIKEN structural genomics initiative (RSGI)
Submitted (NOV-2004) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 38-145.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY151050 Genomic DNA. Translation: AAN84530.1.
AK031738 mRNA. Translation: BAC27531.1.
AK086646 mRNA. Translation: BAC39708.1.
BC008128 mRNA. Translation: AAH08128.1.
BC050775 mRNA. Translation: AAH50775.1.
RefSeqNP_598501.1. NM_133740.2.
UniGeneMm.33202.
Mm.349442.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1WIRNMR-A38-145[»]
ProteinModelPortalQ922H1.
SMRQ922H1. Positions 38-145, 208-532.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ922H1. 1 interaction.
STRING10090.ENSMUSP00000082373.

PTM databases

PhosphoSiteQ922H1.

Proteomic databases

PaxDbQ922H1.
PRIDEQ922H1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000032715; ENSMUSP00000032715; ENSMUSG00000030505. [Q922H1-2]
GeneID71974.
KEGGmmu:71974.
UCSCuc009hbt.1. mouse. [Q922H1-2]

Organism-specific databases

CTD10196.
MGIMGI:1919224. Prmt3.

Phylogenomic databases

eggNOGCOG0500.
GeneTreeENSGT00550000074406.
HOGENOMHOG000198521.
HOVERGENHBG001793.
InParanoidQ922H1.
KOK11436.
OMALCNISLA.
PhylomeDBQ922H1.
TreeFamTF323587.

Gene expression databases

ArrayExpressQ922H1.
BgeeQ922H1.
CleanExMM_PRMT3.
GenevestigatorQ922H1.

Family and domain databases

InterProIPR025799. Arg_MeTrfase.
IPR010456. Ribosomal-L11_MeTrfase_PrmA.
IPR015880. Znf_C2H2-like.
[Graphical view]
PANTHERPTHR11006. PTHR11006. 1 hit.
PfamPF06325. PrmA. 1 hit.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEPS51678. SAM_MT_PRMT. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ922H1.
NextBio335102.
PROQ922H1.
SOURCESearch...

Entry information

Entry nameANM3_MOUSE
AccessionPrimary (citable) accession number: Q922H1
Secondary accession number(s): Q80VU9, Q8BFV5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: June 7, 2004
Last modified: April 16, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot