Reviewed,
UniProtKB/Swiss-Prot Q922D8 (C1TC_MOUSE)
Last modified
February 9, 2010.
Version 71.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: C-1-tetrahydrofolate synthase, cytoplasmic Short name=C1-THF synthase Including the following 3 domains: 1- Recommended name: Methylenetetrahydrofolate dehydrogenase EC=1.5.1.5 2- Recommended name: Methenyltetrahydrofolate cyclohydrolase EC=3.5.4.9 3- Recommended name: Formyltetrahydrofolate synthetase EC=6.3.4.3 | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 935 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | 5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH. 5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate. ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. |
| Pathway | |
| Subunit structure | Homodimer By similarity. |
| Subcellular location | Cytoplasm By similarity. |
| Domain | This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity. |
| Disruption phenotype | Knockout mice have revealed Mthfd1l as a monofunctional enzyme having only formyltetrahydrofolate synthetase activity and lacking methenyl-THF dehydrogenase and cyclohydrolase activities. Ref.3 |
| Sequence similarities | In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family. In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed By similarity | ||||||
| Chain | 2 – 935 | 934 | C-1-tetrahydrofolate synthase, cytoplasmic | PRO_0000199322 | |||||
Regions | |||||||||
| Nucleotide binding | 172 – 174 | 3 | NADP By similarity | ||||||
| Nucleotide binding | 380 – 387 | 8 | ATP By similarity | ||||||
| Region | 2 – 305 | 304 | Methylenetetrahydrofolate dehydrogenase and cyclohydrolase | ||||||
| Region | 52 – 56 | 5 | Substrate binding By similarity | ||||||
| Region | 99 – 101 | 3 | Substrate binding By similarity | ||||||
| Region | 272 – 276 | 5 | Substrate binding By similarity | ||||||
| Region | 306 – 935 | 630 | Formyltetrahydrofolate synthetase | ||||||
Sites | |||||||||
| Binding site | 197 | 1 | NADP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 553 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 786 | 1 | Phosphothreonine Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [2] | Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-17; 27-56; 59-66; 75-83; 123-134; 138-173; 176-198; 205-223; 251-262; 314-324; 330-352; 355-362; 371-402; 464-473; 488-495; 505-517; 521-532; 543-553; 658-679; 687-702; 706-733; 747-772; 776-784; 805-819; 833-848 AND 855-889, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus. |
| [3] | "Disruption of the mthfd1 gene reveals a monofunctional 10-formyltetrahydrofolate synthetase in mammalian mitochondria." Christensen K.E., Patel H., Kuzmanov U., Mejia N.R., MacKenzie R.E. J. Biol. Chem. 280:7597-7602(2005) [PubMed: 15611115] [Abstract] Cited for: DISRUPTION PHENOTYPE. |
| [4] | "Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-786, MASS SPECTROMETRY. Tissue: Liver. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BC008523 mRNA. Translation: AAH08523.1. |
| IPI | IPI00122862. |
| UniGene | Mm.29584 |
3D structure databases | |
| SMR | Q922D8. Positions 2-296, 469-531. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q922D8. |
PTM databases | |
| PhosphoSite | Q922D8. |
2-D gel databases | |
| REPRODUCTION-2DPAGE | Q922D8. |
Proteomic databases | |
| PRIDE | Q922D8. |
Genome annotation databases | |
| Ensembl | ENSMUST00000021443; ENSMUSP00000021443; ENSMUSG00000021048; Mus musculus. [Genome view] |
| UCSC | uc007nxz.1. mouse. |
Organism-specific databases | |
| MGI | MGI:1342005. Mthfd1. |
Phylogenomic databases | |
| HOVERGEN | Q922D8. |
| InParanoid | Q922D8. |
| PhylomeDB | Q922D8. |
Enzyme and pathway databases | |
| BRENDA | 1.5.1.5. 244. 3.5.4.9. 244. 6.3.4.3. 244. |
Gene expression databases | |
| ArrayExpress | Q922D8. |
| Bgee | Q922D8. |
| Genevestigator | Q922D8. |
| GermOnline | ENSMUSG00000021048. Mus musculus. |
Family and domain databases | |
| InterPro | IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. IPR016040. NAD(P)-bd_dom. IPR000672. THF_DH/CycHdrlase. IPR020630. THF_DH/CycHdrlase_cat_dom. IPR020867. THF_DH/CycHdrlase_CS. IPR020631. THF_DH/CycHdrlase_NAD-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| Pfam | PF01268. FTHFS. 1 hit. PF00763. THF_DHG_CYH. 1 hit. PF02882. THF_DHG_CYH_C. 1 hit. [Graphical view] |
| PRINTS | PR00085. THFDHDRGNASE. |
| PROSITE | PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. PS00766. THF_DHG_CYH_1. 1 hit. PS00767. THF_DHG_CYH_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 360186. |
| SOURCE | Search... |
Entry information
| Entry name | C1TC_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q922D8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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