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Protein

Sperm-specific antigen 2 homolog

Gene

Ssfa2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Sperm-specific antigen 2 homolog
Alternative name(s):
Ki-ras-induced actin-interacting protein
Gene namesi
Name:Ssfa2
Synonyms:Kiaa1927, Krap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1917849. Ssfa2.

Subcellular locationi

  • Cytoplasm 1 Publication

  • Note: Located near the plasma membrane. Associated with actin filaments. May also exist as a membrane-bound form with extracellular regions (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12521252Sperm-specific antigen 2 homologPRO_0000072212Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei85 – 851PhosphothreonineCombined sources
Modified residuei90 – 901PhosphoserineCombined sources
Modified residuei109 – 1091PhosphoserineCombined sources
Modified residuei268 – 2681PhosphoserineCombined sources
Modified residuei328 – 3281PhosphoserineBy similarity
Modified residuei465 – 4651PhosphoserineCombined sources
Modified residuei643 – 6431PhosphoserineBy similarity
Modified residuei667 – 6671PhosphoserineCombined sources
Modified residuei736 – 7361PhosphoserineCombined sources
Modified residuei738 – 7381PhosphoserineCombined sources
Modified residuei745 – 7451PhosphoserineCombined sources
Modified residuei758 – 7581PhosphoserineBy similarity
Cross-linki807 – 807Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei866 – 8661PhosphoserineCombined sources
Modified residuei898 – 8981PhosphoserineBy similarity
Modified residuei1036 – 10361PhosphoserineCombined sources
Modified residuei1051 – 10511PhosphoserineCombined sources
Modified residuei1056 – 10561PhosphoserineCombined sources
Modified residuei1059 – 10591PhosphoserineCombined sources
Modified residuei1114 – 11141PhosphoserineBy similarity
Modified residuei1154 – 11541PhosphoserineCombined sources
Modified residuei1161 – 11611PhosphothreonineBy similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ922B9.
MaxQBiQ922B9.
PaxDbiQ922B9.
PRIDEiQ922B9.

PTM databases

iPTMnetiQ922B9.
PhosphoSiteiQ922B9.

Expressioni

Gene expression databases

BgeeiQ922B9.
CleanExiMM_SSFA2.
ExpressionAtlasiQ922B9. baseline and differential.
GenevisibleiQ922B9. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000107415.

Structurei

3D structure databases

ProteinModelPortaliQ922B9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili955 – 103177Sequence analysisAdd
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IERU. Eukaryota.
ENOG410ZSFW. LUCA.
GeneTreeiENSGT00530000063345.
HOVERGENiHBG082646.
InParanoidiQ922B9.
OMAiQSTCSLH.
OrthoDBiEOG7S21X2.
TreeFamiTF331566.

Family and domain databases

InterProiIPR029325. IP3R-bd.
IPR026648. SSFA2.
IPR029326. SSFA2_C.
[Graphical view]
PANTHERiPTHR17469:SF11. PTHR17469:SF11. 3 hits.
PfamiPF14722. KRAP_IP3R_bind. 1 hit.
PF14723. SSFA2_C. 1 hit.
[Graphical view]
SMARTiSM01257. KRAP_IP3R_bind. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q922B9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNRPLSAEAE EELEWQVASR RRKAWAKCRS SWQASETEDL STETTTQDED
60 70 80 90 100
EDDEEDLPGT KLPAPAGRGN VPNEKIAIWL KDCRTPLGAS LDEQSSGTPK
110 120 130 140 150
GVLVRNGGSF EDDLSLGAEA NHLHEPDAQV ENCNNILAKE RRLQFHQKGR
160 170 180 190 200
SMNSTGSGKS SGTVSSVSEL LELYEEDPEE ILYNLGFGRD EPDIASKIPS
210 220 230 240 250
RFFNSSSFAR GIDIKVFLSA QMQRMEVENP NYALTSRFRQ IEVLTTVANA
260 270 280 290 300
FSSLYSQVSG TPLQRIGSMS SVTSTKEVAD SPPPLTRSNT ANRLMKTLSK
310 320 330 340 350
LNLCVDKTEK GEGGSSPATE KGRTLSISLS EDGGGGKSDP KLQKVVKKKE
360 370 380 390 400
SSSMLATVTE EVSGSSSTVT DSVDADRLSE EADSTISHQE ESEESREAHS
410 420 430 440 450
QEKDPLRKSA VTDPDLGHDG RVSSHCELES SSELKSAQAS SSEKEPCAPL
460 470 480 490 500
TIPSIRNIMT QQKDSFEMEE VQSTEGEAPH VPATCQLSLA KSKRDHLLRT
510 520 530 540 550
ASQHSDSSGF AEDSTDCVSL NHLLVNESLQ AMGSSADSCD SETTVTSLGE
560 570 580 590 600
DHVTPTAQDQ PYFNESEEES LAPLQKGRAK VEIVAEKRKA DNQDFPQCVT
610 620 630 640 650
AENAGNNEST KGPCEPGHQI TETGEHPPLA ATGELPREES VESDVEKGSE
660 670 680 690 700
CEFAQYTTHH ILRSLASFEA QGSGMSSEKK TGFPSSVDRV NTALQRAQMK
710 720 730 740 750
VCSMSGQRVG RSLIKSKDLL KQRYLLAKAG YPLRRSQSLP TTLLSPVRVV
760 770 780 790 800
SSVNVRLSPG KETRCSPPSF TYKYTPEEEQ DLEKQGTEHD GQSLVKSTIF
810 820 830 840 850
IPPPSVKKEE APQSEGTRLE ECHHGRLAPC PQFAPISQST CSLHSVHSEW
860 870 880 890 900
QDRPLCEHMR TLSAHSVPNI SGAACSAFSP FGCPYSHRHA AHPYRACSVN
910 920 930 940 950
PPSAIEMQLR RVLHDIRSSL QNLSQYPMTR GPDLAAAPYS TQNSSVLPLY
960 970 980 990 1000
ENTFQELQVV RRSLNLFRTQ MMDLELAMLR QQTVVYPHMT EEDRYEVDQL
1010 1020 1030 1040 1050
QGLRNSVRME LQDLEMQLEE RLLGLDEQLR AVRVPSPFRP SALPGMCGSR
1060 1070 1080 1090 1100
SVDNLSCPSP LNVMEPVTEL IREQSYLKSE LGLGLGDMAY EIPPGESSES
1110 1120 1130 1140 1150
VFSQATSESS SVCSSPSHTN RRSRGLPGSK PRARLVARKK IFRASVALTP
1160 1170 1180 1190 1200
TAPSRTGSVQ TPPDLESSEE AGGAEEASPV VGLASHVEEE PEDLSLMPAA
1210 1220 1230 1240 1250
EEMHRNVEQD ELQQVIREIK ESIVGEIRRE IVSGLLAAVS SSKAPGPKQD

SH
Length:1,252
Mass (Da):136,947
Last modified:July 27, 2011 - v3
Checksum:i2FA8C5874C1A573E
GO
Isoform 2 (identifier: Q922B9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1063: Missing.

Note: No experimental confirmation available.
Show »
Length:189
Mass (Da):20,231
Checksum:iCF2713BFF32BE690
GO
Isoform 3 (identifier: Q922B9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-151: Missing.

Show »
Length:1,101
Mass (Da):120,168
Checksum:i1399760C3C962FA9
GO

Sequence cautioni

The sequence AAH08560.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH18526.3 differs from that shown. Reason: Erroneous termination at position 1249. Translated as Gln.Curated
The sequence AAI22520.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC29756.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti319 – 3191T → A in BAC87833 (PubMed:14673706).Curated
Sequence conflicti319 – 3191T → A in AAI22520 (PubMed:15489334).Curated
Sequence conflicti319 – 3191T → A in AAI41884 (PubMed:15489334).Curated
Sequence conflicti319 – 3191T → A in BAC41485 (PubMed:12465718).Curated
Sequence conflicti1071 – 10711I → M in BAC87833 (PubMed:14673706).Curated
Sequence conflicti1071 – 10711I → M in AAH18526 (PubMed:15489334).Curated
Sequence conflicti1071 – 10711I → M in AAI41884 (PubMed:15489334).Curated
Sequence conflicti1071 – 10711I → M in AAI22520 (PubMed:15489334).Curated
Sequence conflicti1071 – 10711I → M in BAC41485 (PubMed:12465718).Curated
Sequence conflicti1240 – 12401S → A in BAC87833 (PubMed:14673706).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 10631063Missing in isoform 2. 1 PublicationVSP_023869Add
BLAST
Alternative sequencei1 – 151151Missing in isoform 3. 1 PublicationVSP_023868Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB120565 mRNA. Translation: BAC87833.1.
AL844577, AL928770 Genomic DNA. Translation: CAM14505.1.
AL928770, AL844577 Genomic DNA. Translation: CAM17563.1.
BC008560 mRNA. Translation: AAH08560.1. Different initiation.
BC018526 mRNA. Translation: AAH18526.3. Different termination.
BC079535 mRNA. Translation: AAH79535.1.
BC115799 mRNA. Translation: AAI15800.1.
BC122519 mRNA. Translation: AAI22520.1. Different initiation.
BC141883 mRNA. Translation: AAI41884.1.
AK037213 mRNA. Translation: BAC29756.1. Different initiation.
AB093303 mRNA. Translation: BAC41485.1.
CCDSiCCDS16170.1. [Q922B9-1]
RefSeqiNP_542125.3. NM_080558.4. [Q922B9-1]
UniGeneiMm.272881.

Genome annotation databases

EnsembliENSMUST00000111785; ENSMUSP00000107415; ENSMUSG00000027007. [Q922B9-1]
GeneIDi70599.
KEGGimmu:70599.
UCSCiuc008kgw.2. mouse. [Q922B9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB120565 mRNA. Translation: BAC87833.1.
AL844577, AL928770 Genomic DNA. Translation: CAM14505.1.
AL928770, AL844577 Genomic DNA. Translation: CAM17563.1.
BC008560 mRNA. Translation: AAH08560.1. Different initiation.
BC018526 mRNA. Translation: AAH18526.3. Different termination.
BC079535 mRNA. Translation: AAH79535.1.
BC115799 mRNA. Translation: AAI15800.1.
BC122519 mRNA. Translation: AAI22520.1. Different initiation.
BC141883 mRNA. Translation: AAI41884.1.
AK037213 mRNA. Translation: BAC29756.1. Different initiation.
AB093303 mRNA. Translation: BAC41485.1.
CCDSiCCDS16170.1. [Q922B9-1]
RefSeqiNP_542125.3. NM_080558.4. [Q922B9-1]
UniGeneiMm.272881.

3D structure databases

ProteinModelPortaliQ922B9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000107415.

PTM databases

iPTMnetiQ922B9.
PhosphoSiteiQ922B9.

Proteomic databases

EPDiQ922B9.
MaxQBiQ922B9.
PaxDbiQ922B9.
PRIDEiQ922B9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000111785; ENSMUSP00000107415; ENSMUSG00000027007. [Q922B9-1]
GeneIDi70599.
KEGGimmu:70599.
UCSCiuc008kgw.2. mouse. [Q922B9-1]

Organism-specific databases

CTDi6744.
MGIiMGI:1917849. Ssfa2.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IERU. Eukaryota.
ENOG410ZSFW. LUCA.
GeneTreeiENSGT00530000063345.
HOVERGENiHBG082646.
InParanoidiQ922B9.
OMAiQSTCSLH.
OrthoDBiEOG7S21X2.
TreeFamiTF331566.

Miscellaneous databases

ChiTaRSiSsfa2. mouse.
NextBioi331942.
PROiQ922B9.
SOURCEiSearch...

Gene expression databases

BgeeiQ922B9.
CleanExiMM_SSFA2.
ExpressionAtlasiQ922B9. baseline and differential.
GenevisibleiQ922B9. MM.

Family and domain databases

InterProiIPR029325. IP3R-bd.
IPR026648. SSFA2.
IPR029326. SSFA2_C.
[Graphical view]
PANTHERiPTHR17469:SF11. PTHR17469:SF11. 3 hits.
PfamiPF14722. KRAP_IP3R_bind. 1 hit.
PF14723. SSFA2_C. 1 hit.
[Graphical view]
SMARTiSM01257. KRAP_IP3R_bind. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Deregulated expression of KRAP, a novel gene encoding actin-interacting protein, in human colon cancer cells."
    Inokuchi J., Komiya M., Baba I., Naito S., Sasazuki T., Shirasawa S.
    J. Hum. Genet. 49:46-52(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Brain.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Skin.
  5. "Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O., Koga H.
    DNA Res. 9:179-188(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-1252.
    Tissue: Brain.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-85; SER-90; SER-736 AND SER-738, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-90, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-90; SER-109; SER-268; SER-465; SER-667; SER-736; SER-738; SER-745; SER-866; SER-1036; SER-1051; SER-1056; SER-1059 AND SER-1154, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiSSFA2_MOUSE
AccessioniPrimary (citable) accession number: Q922B9
Secondary accession number(s): A2AQD4
, A5D8Z6, Q08E80, Q14BJ9, Q544I3, Q68FN1, Q75WU7, Q8CH96, Q8VEF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.