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Protein

Aspartate--tRNA ligase, cytoplasmic

Gene

Dars

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.By similarity

Catalytic activityi

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei229AspartateBy similarity1
Binding sitei273AspartateBy similarity1
Binding sitei424ATPBy similarity1
Binding sitei427AspartateBy similarity1
Binding sitei431AspartateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi273 – 275ATPBy similarity3
Nucleotide bindingi281 – 283ATPBy similarity3
Nucleotide bindingi472 – 475ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.1.1.12 3474

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA ligase, cytoplasmic (EC:6.1.1.12By similarity)
Alternative name(s):
Aspartyl-tRNA synthetase
Short name:
AspRS
Gene namesi
Name:Dars
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2442544 Dars

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001110111 – 501Aspartate--tRNA ligase, cytoplasmicAdd BLAST501

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei52PhosphothreonineBy similarity1
Modified residuei74N6-acetyllysineBy similarity1
Modified residuei249PhosphoserineBy similarity1
Modified residuei374N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ922B2
MaxQBiQ922B2
PaxDbiQ922B2
PeptideAtlasiQ922B2
PRIDEiQ922B2

PTM databases

iPTMnetiQ922B2
PhosphoSitePlusiQ922B2
SwissPalmiQ922B2

Expressioni

Gene expression databases

BgeeiENSMUSG00000026356
CleanExiMM_DARS
ExpressionAtlasiQ922B2 baseline and differential
GenevisibleiQ922B2 MM

Interactioni

Subunit structurei

Homodimer (By similarity). Part of a multisubunit complex that groups tRNA ligases for Arg (RARS), Asp (DARS), Gln (QARS), Ile (IARS), Leu (LARS), Lys (KARS), Met (MARS) the bifunctional ligase for Glu and Pro (EPRS) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18 (PubMed:12060739).By similarity1 Publication

Protein-protein interaction databases

BioGridi230508, 3 interactors
IntActiQ922B2, 2 interactors
MINTiQ922B2
STRINGi10090.ENSMUSP00000027602

Structurei

3D structure databases

ProteinModelPortaliQ922B2
SMRiQ922B2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni251 – 254AspartateBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0556 Eukaryota
COG0017 LUCA
GeneTreeiENSGT00550000074880
HOGENOMiHOG000226032
HOVERGENiHBG001028
InParanoidiQ922B2
KOiK22503
OMAiWVHEIRD
OrthoDBiEOG091G0530
TreeFamiTF105676

Family and domain databases

HAMAPiMF_02075 Asp_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR004364 aa-tRNA-synt_II
IPR006195 aa-tRNA-synth_II
IPR004523 Asp-tRNA_synthase_2
IPR002312 Asp/Asn-tRNA-synth_IIb
IPR012340 NA-bd_OB-fold
IPR004365 NA-bd_OB_tRNA
PANTHERiPTHR43450 PTHR43450, 1 hit
PfamiView protein in Pfam
PF00152 tRNA-synt_2, 1 hit
PF01336 tRNA_anti-codon, 1 hit
PRINTSiPR01042 TRNASYNTHASP
SUPFAMiSSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00458 aspS_nondisc, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

Q922B2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSTNASRKG QEKPREIVDA AEDYAKERYG ISSMIQSQEK PDRVLVRVKD
60 70 80 90 100
LTVQKADDVV WVRARVHTSR AKGKQCFLVL RQQQFNVQAL VAVGDHASKQ
110 120 130 140 150
MVKFAANINK ESIIDVEGVV RKVNQKIGSC TQQDVELHVQ KIYVISLAEP
160 170 180 190 200
RLPLQLDDAI RPEVEGEEDG RATVNQDTRL DNRVIDLRTS TSQAIFRLQS
210 220 230 240 250
GICHLFRETL INKGFVEIQT PKIISAASEG GANVFTVSYF KNNAYLAQSP
260 270 280 290 300
QLYKQMCICA DFEKVFCIGP VFRAEDSNTH RHLTEFVGLD IEMAFNYHYH
310 320 330 340 350
EVVEEIADTL VQIFKGLQER FQTEIQTVSK QFPCEPFKFL EPTLRLEYCE
360 370 380 390 400
ALAMLREAGV EMDDEEDLST PNEKLLGRLV KEKYDTDFYV LDKYPLAVRP
410 420 430 440 450
FYTMPDPRNP KQSNSYDMFM RGEEILSGAQ RIHDPQLLTE RALHHGIDLE
460 470 480 490 500
KIKAYIDSFR FGAPPHAGGG IGLERVTMLF LGLHNVRQTS MFPRDPKRLT

P
Length:501
Mass (Da):57,147
Last modified:July 27, 2011 - v2
Checksum:iFC309A0CE55D36E6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4T → A in AAH08638 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK151027 mRNA Translation: BAE30045.1
AK151897 mRNA Translation: BAE30780.1
AK152304 mRNA Translation: BAE31110.1
AK152562 mRNA Translation: BAE31315.1
AK153256 mRNA Translation: BAE31847.1
AK153299 mRNA Translation: BAE31881.1
AK169716 mRNA Translation: BAE41327.1
BC008638 mRNA Translation: AAH08638.1
CCDSiCCDS15253.1
RefSeqiNP_803228.2, NM_177445.5
UniGeneiMm.28693

Genome annotation databases

EnsembliENSMUST00000027602; ENSMUSP00000027602; ENSMUSG00000026356
GeneIDi226414
KEGGimmu:226414
UCSCiuc007clp.2 mouse

Similar proteinsi

Entry informationi

Entry nameiSYDC_MOUSE
AccessioniPrimary (citable) accession number: Q922B2
Secondary accession number(s): Q3U987
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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