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Protein

Aspartate--tRNA ligase, cytoplasmic

Gene

Dars

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.

Catalytic activityi

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei229AspartateBy similarity1
Binding sitei273AspartateBy similarity1
Binding sitei424ATPBy similarity1
Binding sitei427AspartateBy similarity1
Binding sitei431AspartateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi273 – 275ATPBy similarity3
Nucleotide bindingi281 – 283ATPBy similarity3
Nucleotide bindingi472 – 475ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.1.1.12. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA ligase, cytoplasmic (EC:6.1.1.12)
Alternative name(s):
Aspartyl-tRNA synthetase
Short name:
AspRS
Gene namesi
Name:Dars
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2442544. Dars.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001110111 – 501Aspartate--tRNA ligase, cytoplasmicAdd BLAST501

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei52PhosphothreonineBy similarity1
Modified residuei74N6-acetyllysineBy similarity1
Modified residuei249PhosphoserineBy similarity1
Modified residuei374N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ922B2.
MaxQBiQ922B2.
PaxDbiQ922B2.
PeptideAtlasiQ922B2.
PRIDEiQ922B2.

PTM databases

iPTMnetiQ922B2.
PhosphoSitePlusiQ922B2.
SwissPalmiQ922B2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026356.
CleanExiMM_DARS.
ExpressionAtlasiQ922B2. baseline and differential.
GenevisibleiQ922B2. MM.

Interactioni

Subunit structurei

Homodimer; also part of a multisubunit complex that groups AIMP1, AIMP2, EEF1A1 and tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro.

Protein-protein interaction databases

BioGridi230508. 3 interactors.
IntActiQ922B2. 2 interactors.
MINTiMINT-1856918.
STRINGi10090.ENSMUSP00000027602.

Structurei

3D structure databases

ProteinModelPortaliQ922B2.
SMRiQ922B2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni251 – 254AspartateBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0556. Eukaryota.
COG0017. LUCA.
GeneTreeiENSGT00550000074880.
HOGENOMiHOG000226032.
HOVERGENiHBG001028.
InParanoidiQ922B2.
KOiK01876.
OMAiWGDDLST.
OrthoDBiEOG091G0530.
TreeFamiTF105676.

Family and domain databases

HAMAPiMF_02075. Asp_tRNA_synth_type2. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004523. Asp-tRNA_synthase_arc.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF33. PTHR22594:SF33. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00458. aspS_nondisc. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q922B2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSTNASRKG QEKPREIVDA AEDYAKERYG ISSMIQSQEK PDRVLVRVKD
60 70 80 90 100
LTVQKADDVV WVRARVHTSR AKGKQCFLVL RQQQFNVQAL VAVGDHASKQ
110 120 130 140 150
MVKFAANINK ESIIDVEGVV RKVNQKIGSC TQQDVELHVQ KIYVISLAEP
160 170 180 190 200
RLPLQLDDAI RPEVEGEEDG RATVNQDTRL DNRVIDLRTS TSQAIFRLQS
210 220 230 240 250
GICHLFRETL INKGFVEIQT PKIISAASEG GANVFTVSYF KNNAYLAQSP
260 270 280 290 300
QLYKQMCICA DFEKVFCIGP VFRAEDSNTH RHLTEFVGLD IEMAFNYHYH
310 320 330 340 350
EVVEEIADTL VQIFKGLQER FQTEIQTVSK QFPCEPFKFL EPTLRLEYCE
360 370 380 390 400
ALAMLREAGV EMDDEEDLST PNEKLLGRLV KEKYDTDFYV LDKYPLAVRP
410 420 430 440 450
FYTMPDPRNP KQSNSYDMFM RGEEILSGAQ RIHDPQLLTE RALHHGIDLE
460 470 480 490 500
KIKAYIDSFR FGAPPHAGGG IGLERVTMLF LGLHNVRQTS MFPRDPKRLT

P
Length:501
Mass (Da):57,147
Last modified:July 27, 2011 - v2
Checksum:iFC309A0CE55D36E6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4T → A in AAH08638 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK151027 mRNA. Translation: BAE30045.1.
AK151897 mRNA. Translation: BAE30780.1.
AK152304 mRNA. Translation: BAE31110.1.
AK152562 mRNA. Translation: BAE31315.1.
AK153256 mRNA. Translation: BAE31847.1.
AK153299 mRNA. Translation: BAE31881.1.
AK169716 mRNA. Translation: BAE41327.1.
BC008638 mRNA. Translation: AAH08638.1.
CCDSiCCDS15253.1.
RefSeqiNP_803228.2. NM_177445.5.
UniGeneiMm.28693.

Genome annotation databases

EnsembliENSMUST00000027602; ENSMUSP00000027602; ENSMUSG00000026356.
GeneIDi226414.
KEGGimmu:226414.
UCSCiuc007clp.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK151027 mRNA. Translation: BAE30045.1.
AK151897 mRNA. Translation: BAE30780.1.
AK152304 mRNA. Translation: BAE31110.1.
AK152562 mRNA. Translation: BAE31315.1.
AK153256 mRNA. Translation: BAE31847.1.
AK153299 mRNA. Translation: BAE31881.1.
AK169716 mRNA. Translation: BAE41327.1.
BC008638 mRNA. Translation: AAH08638.1.
CCDSiCCDS15253.1.
RefSeqiNP_803228.2. NM_177445.5.
UniGeneiMm.28693.

3D structure databases

ProteinModelPortaliQ922B2.
SMRiQ922B2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230508. 3 interactors.
IntActiQ922B2. 2 interactors.
MINTiMINT-1856918.
STRINGi10090.ENSMUSP00000027602.

PTM databases

iPTMnetiQ922B2.
PhosphoSitePlusiQ922B2.
SwissPalmiQ922B2.

Proteomic databases

EPDiQ922B2.
MaxQBiQ922B2.
PaxDbiQ922B2.
PeptideAtlasiQ922B2.
PRIDEiQ922B2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027602; ENSMUSP00000027602; ENSMUSG00000026356.
GeneIDi226414.
KEGGimmu:226414.
UCSCiuc007clp.2. mouse.

Organism-specific databases

CTDi1615.
MGIiMGI:2442544. Dars.

Phylogenomic databases

eggNOGiKOG0556. Eukaryota.
COG0017. LUCA.
GeneTreeiENSGT00550000074880.
HOGENOMiHOG000226032.
HOVERGENiHBG001028.
InParanoidiQ922B2.
KOiK01876.
OMAiWGDDLST.
OrthoDBiEOG091G0530.
TreeFamiTF105676.

Enzyme and pathway databases

BRENDAi6.1.1.12. 3474.

Miscellaneous databases

PROiQ922B2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026356.
CleanExiMM_DARS.
ExpressionAtlasiQ922B2. baseline and differential.
GenevisibleiQ922B2. MM.

Family and domain databases

HAMAPiMF_02075. Asp_tRNA_synth_type2. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004523. Asp-tRNA_synthase_arc.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF33. PTHR22594:SF33. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00458. aspS_nondisc. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYDC_MOUSE
AccessioniPrimary (citable) accession number: Q922B2
Secondary accession number(s): Q3U987
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.