Reviewed, UniProtKB/Swiss-Prot Q922B2 (SYDC_MOUSE)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:

Dars

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).
Subunit structure	Homodimer; also part of a multisubunit complex that groups tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Aspartyl-tRNA synthetase, cytoplasmic		PRO_0000111011
Amino acid modifications
	Modified residue	74	1	N6-acetyllysine By similarity
	Modified residue	238	1	Phosphoserine By similarity
	Modified residue	374	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q922B2-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 1C3430164FF79C4C	FASTA	501	57,117
	10 20 30 40 50 60 MPSANASRKG QEKPREIVDA AEDYAKERYG ISSMIQSQEK PDRVLVRVKD LTVQKADDVV 70 80 90 100 110 120 WVRARVHTSR AKGKQCFLVL RQQQFNVQAL VAVGDHASKQ MVKFAANINK ESIIDVEGVV 130 140 150 160 170 180 RKVNQKIGSC TQQDVELHVQ KIYVISLAEP RLPLQLDDAI RPEVEGEEDG RATVNQDTRL 190 200 210 220 230 240 DNRVIDLRTS TSQAIFRLQS GICHLFRETL INKGFVEIQT PKIISAASEG GANVFTVSYF 250 260 270 280 290 300 KNNAYLAQSP QLYKQMCICA DFEKVFCIGP VFRAEDSNTH RHLTEFVGLD IEMAFNYHYH 310 320 330 340 350 360 EVVEEIADTL VQIFKGLQER FQTEIQTVSK QFPCEPFKFL EPTLRLEYCE ALAMLREAGV 370 380 390 400 410 420 EMDDEEDLST PNEKLLGRLV KEKYDTDFYV LDKYPLAVRP FYTMPDPRNP KQSNSYDMFM 430 440 450 460 470 480 RGEEILSGAQ RIHDPQLLTE RALHHGIDLE KIKAYIDSFR FGAPPHAGGG IGLERVTMLF 490 500 LGLHNVRQTS MFPRDPKRLT P

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC008638 mRNA. Translation: AAH08638.1.
IPI	IPI00122743.
UniGene	Mm.28693 Mm.431446
3D structure databases
HSSP	HSSP built from PDB template 1ASZ based on UniProtKB P04802.
ModBase	Search...
Protein-protein interaction databases
STRING	Q922B2.
PTM databases
PhosphoSite	Q922B2.
Proteomic databases
PRIDE	Q922B2.
Genome annotation databases
Ensembl	ENSMUST00000027602; ENSMUSP00000027602; ENSMUSG00000026356; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:2442544. Dars.
Phylogenomic databases
HOVERGEN	Q922B2.
Enzyme and pathway databases
BRENDA	6.1.1.12. 244.
Gene expression databases
ArrayExpress	Q922B2.
Bgee	Q922B2.
CleanEx	MM_DARS.
Genevestigator	Q922B2.
GermOnline	ENSMUSG00000026356. Mus musculus.
Family and domain databases
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002312. Asp-tRNA-synth_IIb. IPR004523. Asp-tRNA-synth_IIb_arc/euk. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00458. aspS_arch. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	378138.
SOURCE	Search...

Entry information

Entry name

SYDC_MOUSE

Accession

Primary (citable) accession number: Q922B2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	December 1, 2001
Last modified:	October 13, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)