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Protein

Negative regulator of the PHO system

Gene

PHO85

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

When phosphate concentrations are high it phosphorylates the PHO4 transcription factor thus establishing repression.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36ATPPROSITE-ProRule annotation1
Active sitei133Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 21ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Negative regulator of the PHO system (EC:2.7.11.22)
Alternative name(s):
Serine/threonine-protein kinase PHO85
Gene namesi
Name:PHO85
Ordered Locus Names:KLLA0D11990g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome D

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000865191 – 304Negative regulator of the PHO systemAdd BLAST304

Interactioni

Subunit structurei

Interacts with a number of cyclins.By similarity

Protein-protein interaction databases

STRINGi284590.XP_453596.1.

Structurei

3D structure databases

ProteinModelPortaliQ92241.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 297Protein kinasePROSITE-ProRule annotationAdd BLAST291

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0594. Eukaryota.
ENOG410XPP3. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ92241.
KOiK06655.
OMAiPNFHVYA.
OrthoDBiEOG092C2FL8.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92241-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAPSQFKQL EKVGNGTYAT VYKGLNKTTG VYVALKEVKL DSEEGTPSTA
60 70 80 90 100
IREISLMKEL KHDNIVRLFD VIHTENKLTL VFEFMDNDLK KFMDNRNKGN
110 120 130 140 150
SHKGLEMDLV KYFQWQLLQG VAFCHENRIL HRDLKPQNLL INNRGQLKLG
160 170 180 190 200
DFGLARAFGI PVNTFSSEVV TLWYRAPDVL MGSRNYCTSI DIWSCGCILA
210 220 230 240 250
EMIMGKPLFP GSNDEEQLKL IFDTMGTPVE QTWPQVTQLA KYNPLLPPHM
260 270 280 290 300
PRDLKQLLQN NTEEVLDDNV VDLLHGLLQL NPDARLSAKD ALNHPWFAEY

NHAN
Length:304
Mass (Da):34,658
Last modified:September 27, 2004 - v2
Checksum:iCC1BD5B7A9997BC7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti101S → P in CAA64698 (Ref. 1) Curated1
Sequence conflicti189S → L in CAA64698 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95418 Genomic DNA. Translation: CAA64698.1.
CR382124 Genomic DNA. Translation: CAH00692.1.
RefSeqiXP_453596.1. XM_453596.1.

Genome annotation databases

EnsemblFungiiCAH00692; CAH00692; KLLA0_D11990g.
GeneIDi2893430.
KEGGikla:KLLA0D11990g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95418 Genomic DNA. Translation: CAA64698.1.
CR382124 Genomic DNA. Translation: CAH00692.1.
RefSeqiXP_453596.1. XM_453596.1.

3D structure databases

ProteinModelPortaliQ92241.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi284590.XP_453596.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAH00692; CAH00692; KLLA0_D11990g.
GeneIDi2893430.
KEGGikla:KLLA0D11990g.

Phylogenomic databases

eggNOGiKOG0594. Eukaryota.
ENOG410XPP3. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ92241.
KOiK06655.
OMAiPNFHVYA.
OrthoDBiEOG092C2FL8.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHO85_KLULA
AccessioniPrimary (citable) accession number: Q92241
Secondary accession number(s): Q6CR43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 27, 2004
Last modified: October 5, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.