Negative regulator of the PHO system - Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Negative regulator of the PHO system
EC=2.7.11.22

Alternative name(s):
Serine/threonine-protein kinase PHO85

Gene names

Name:	PHO85
Ordered Locus Names:	KLLA0D11990g

Organism

Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

284590 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces ›

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	When phosphate concentrations are high it phosphorylates the PHO4 transcription factor thus establishing repression By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with a number of cyclins By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 304

304

Negative regulator of the PHO system

PRO_0000086519

Regions

Domain

7 – 297

291

Protein kinase

Nucleotide binding

13 – 21

9

ATP By similarity

Sites

Active site

133

1

Proton acceptor By similarity

Binding site

36

1

ATP By similarity

Experimental info

Sequence conflict

101

1

S → P in CAA64698. Ref.1

Sequence conflict

189

1

S → L in CAA64698. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q92241 [UniParc].

Last modified September 27, 2004. Version 2.
Checksum: CC1BD5B7A9997BC7
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FASTA

304

34,658

        10         20         30         40         50         60 
MVAPSQFKQL EKVGNGTYAT VYKGLNKTTG VYVALKEVKL DSEEGTPSTA IREISLMKEL 

        70         80         90        100        110        120 
KHDNIVRLFD VIHTENKLTL VFEFMDNDLK KFMDNRNKGN SHKGLEMDLV KYFQWQLLQG 

       130        140        150        160        170        180 
VAFCHENRIL HRDLKPQNLL INNRGQLKLG DFGLARAFGI PVNTFSSEVV TLWYRAPDVL 

       190        200        210        220        230        240 
MGSRNYCTSI DIWSCGCILA EMIMGKPLFP GSNDEEQLKL IFDTMGTPVE QTWPQVTQLA 

       250        260        270        280        290        300 
KYNPLLPPHM PRDLKQLLQN NTEEVLDDNV VDLLHGLLQL NPDARLSAKD ALNHPWFAEY 


NHAN

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The PHO85 gene of K.lactis." Uccelletti D., Morlupi A., Palleschi C. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 76492 / CBS 2359/152 / CLIB 210.
[2]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. Souciet J.-L. Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X95418 Genomic DNA. Translation: CAA64698.1. CR382124 Genomic DNA. Translation: CAH00692.1.
RefSeq	XP_453596.1. XM_453596.1.
3D structure databases
ProteinModelPortal	Q92241.
SMR	Q92241. Positions 19-302.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2893430.
KEGG	kla:KLLA0D11990g.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
KO	K06655.
OMA	FRAYNFT.
OrthoDB	EOG4QJVX0.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHO85_KLULA

Accession

Primary (citable) accession number: Q92241
Secondary accession number(s): Q6CR43

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	September 27, 2004
Last modified:	May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families