Reviewed, UniProtKB/Swiss-Prot Q92215 (TOXC_COCCA)

Names and origin

Protein names

Recommended name:
    Putative fatty acid synthase subunit TOXC
Including the following 5 domains:
    1- Recommended name:
            3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase
              EC=4.2.1.61
    2- Recommended name:
            Enoyl-[acyl-carrier-protein] reductase [NADH]
              EC=1.3.1.9
    3- Recommended name:
            [Acyl-carrier-protein] acetyltransferase
              EC=2.3.1.38
    4- Recommended name:
            [Acyl-carrier-protein] malonyltransferase
              EC=2.3.1.39
    5- Recommended name:
            S-acyl fatty acid synthase thioesterase
              EC=3.1.2.14

Gene names

Name:

TOXC

Organism

Cochliobolus carbonum (Bipolaris zeicola)

Taxonomic identifier

5017 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Pleosporaceae › Cochliobolus

Protein attributes

Sequence length	2080 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Essential role in the production of HC-toxin and in pathogenicity of the fungus on maize. May contribute to the synthesis of the decanoic backbone of 2-amino-9,10-epoxi-8-oxodecanoic acid. This protein contains domains similar to those of a fatty acid synthase beta subunit, namely: [acyl-carrier protein] acetyltransferase and malonyltransferase, S-acyl fatty acid synthase thioesterase, enoyl-[acyl-carrier-protein] reductase, and 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase. Ref.1
Catalytic activity	Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier-protein]. Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein]. (3R)-3-hydroxypalmitoyl-[acyl-carrier-protein] = hexadec-2-enoyl-[acyl-carrier-protein] + H2O. Acyl-[acyl-carrier-protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH. Oleoyl-[acyl-carrier-protein] + H2O = [acyl-carrier-protein] + oleate.
Pathway	Mycotoxin biosynthesis; HC-toxin biosynthesis.
Sequence similarities	Belongs to the fungal fatty acid synthetase subunit beta family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Ligand	NAD NADP
Molecular function	Hydrolase Lyase Oxidoreductase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	fatty acid synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity Inferred from electronic annotation. Source: EC [acyl-carrier-protein] S-acetyltransferase activity Inferred from electronic annotation. Source: EC [acyl-carrier-protein] S-malonyltransferase activity Inferred from electronic annotation. Source: EC enoyl-[acyl-carrier-protein] reductase (NADH) activity Inferred from electronic annotation. Source: EC oleoyl-[acyl-carrier-protein] hydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2080	2080	Putative fatty acid synthase subunit TOXC		PRO_0000180291
Regions
	Region	1 – 468	468	Acetyltransferase By similarity
	Region	481 – 869	389	Enoyl reductase By similarity UniProtKB P34229
	Region	1155 – 1644	490	Dehydratase By similarity UniProtKB P34229
	Region	1645 – 1865	221	Malonyl/palmitoyl transferase By similarity UniProtKB P34229
Sites
	Active site	276	1	For acetyltransferase activity By similarity UniProtKB P34229
	Active site	1828	1	For malonyltransferase activity By similarity UniProtKB P34229

Sequences

	Sequence		Length	Mass (Da)
	Q92215-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 424D3C489EF67087	FASTA	2,080	232,268
	10 20 30 40 50 60 MLLTAQIPES LTPFSISHGS LSVSWLLPYR LHCYATRLYR TFEATLAARS DNSEHPITLL 70 80 90 100 110 120 SSVELAAHYM CYVAHETQAN TDRACTQTHD ISKLLLEDFE ATFLRSNDIH TLASALPSSD 130 140 150 160 170 180 SAKDELLRCY YETCFITKHN TPLNESALLK AAREGIVSLY TTFSGQGCGG RYFDELRELF 190 200 210 220 230 240 RLYPSFVGTL ISESGNLFRE LASNPSAGRL FSKGFDIMAW LHHPQTTPDT EYLISAPVSF 250 260 270 280 290 300 PIIGLVQLGH YAVSCRAMGL DPGAFQRSIR GSTGHSQGIV VAAAMSAADS WEAFDRLAIS 310 320 330 340 350 360 CLTVLFWIGV RSQQAAPQMS LSPAQIQDSI DHNEDVPSPM LSIIGLSRLD VQMHIDSINH 370 380 390 400 410 420 YLPQSEHISI SLVNGGRHIV VSGLPSTLYR FNLLLRKIKV PDHSGQPRAT SKQKKAQFSA 430 440 450 460 470 480 RFLPITVPFH SHHLVSVGSV LEEDLKNVFI GSKDLGFPVF NTYTGRDLRA EVIGNIVPAL 490 500 510 520 530 540 VRMVTQYPVF WGAAVEFPGA THILEFGPGG LSGSGALTSH IKNGTGVRVI FAGLTSGSNR 550 560 570 580 590 600 QVGYKQELFA RDTCHVKFAD DWSRKYAPSL VRTSNNSIVV NTKMSRLLGL PPIMVGGMTP 610 620 630 640 650 660 TTAAWGFVAA TMNAGYHIEL AAGGYSDANA FENALLNIQK TTASGRGITV NLIYLSSHAV 670 680 690 700 710 720 NWQIPLLRRL IIDGFRIEGI TIGGGVPSID VAKEYITTLG IKHIGFKPGP TTAIDAVIEI 730 740 750 760 770 780 AQANPTFPVF LQWTGGRSGG HHSNEDFHQP ILETYDRIRQ CDNIILIAGS GFGGAADTYP 790 800 810 820 830 840 YITGEWSLRY DFPPMPFDGC LLGSRVMVAK EARTSPAAKR VIVETEGLND NEWRRTYEEA 850 860 870 880 890 900 AGGIITVQSE MGQPIHKIAT RGVLFWAKLD QMIFSLPKEK RIAELQKHRS WIIKGLNDDF 910 920 930 940 950 960 QKPWFGRDSA DQVVELRDMT YAEVLRRMVQ LLYVKHQRRW IHSSYAVLFK AFVNRLEERF 970 980 990 1000 1010 1020 TTKTVQSYLI QDCKTIDDPY NIITVVLLQY QQAIKETILT PDVEYFLLLC KRRGQKPVPF 1030 1040 1050 1060 1070 1080 VPALDEDFEF FFKKDSLWQS EDLEAVVDQD VGRTCILQGP VAAKYSVKVD EPIAEILGSI 1090 1100 1110 1120 1130 1140 HQGHVTRLRE ERYCATLDSI PFVEYFGGES IQLDMSSLAD GIEQSHNEQA SIYSLPSSLS 1150 1160 1170 1180 1190 1200 MPLPAVDVWM SLLAGKSRSW RHAIMSAGIV IQENKCVANP MRRLFAPAHG IRVQIRKPDV 1210 1220 1230 1240 1250 1260 PSQTEVVLEE QQESGIYEVA VRAGLNEDGE IIVEMFERRN MSDLVVSLPC DSGTKPEYGY 1270 1280 1290 1300 1310 1320 APIREIMEDR NERIRRFYWS IWFGKSHPIL EGSLSDSFEC GKEKITRQHV ESFIQAINNS 1330 1340 1350 1360 1370 1380 TRTHKNFLEP ATNVSISFAI PVAWKAIVKP LFLNALNGDL LQLVHLSNEF RMTPGAEPLK 1390 1400 1410 1420 1430 1440 IGEEVSTVAR INAIMNQDSG KMVEVSAAVL RGKEIVVEII SRFLYRGAFV DFKDTFQWRD 1450 1460 1470 1480 1490 1500 EPLMQIQLAT SKHIAVLRTR EWFVPTQGCN IDLVGHTLTF QMRSLYKFQS KTVFRRIETH 1510 1520 1530 1540 1550 1560 GKVTLELAPQ KIVQVATVQY EVGICHSNTV IEFLDRYGSY SQNSVDFEDP VSVPNNGESL 1570 1580 1590 1600 1610 1620 VICAPSSNEA YARTSGDLNP IHVSRTFAEY AGLPGLITHG MYCSAAIQDL VERLVADGNA 1630 1640 1650 1660 1670 1680 GRIRQFSMSF VGMVLPNQKL EVKLEHIGMV EGMIRLHIEA RAQETGHRVI VGEAKITQKM 1690 1700 1710 1720 1730 1740 TTYVFTGQGS QEKGMGMDLY NQCPAAREVW DRGDKYFLHK YGFAITTIVR DNPKQLTVHF 1750 1760 1770 1780 1790 1800 GGRQGEAIRQ NYINMKVETV AEDGSIQYEK LFKDVDHNTQ FYTFRSPTGL LSATQFTQPA 1810 1820 1830 1840 1850 1860 LSLMARASFE HLQIQGLVDG NCYYAGHSLG EFSALAAVAG IMSVESQALI AFYRGLTMQK 1870 1880 1890 1900 1910 1920 AVNRDESGRS NYSMCAVDPS RISATYDEEA FLTIVREIAA ETGWLLEVVN FNVANKQYVC 1930 1940 1950 1960 1970 1980 AGNLHALDTL AGVTDRLRLL QINASEMEEC LHEIIRQCVQ ETKSKSTPLE LTRGIATIPL 1990 2000 2010 2020 2030 2040 QGIDVPFHST FLRGGVRHFR EFLHENIDKR NINPAKLIGR YIPNVTARSF QISKDYFQYV 2050 2060 2070 2080 YDLTGSSQLR DALKNWDIYE KSNGEESNGV EECSECRNSL

References

[1]

"A fatty acid synthase gene in Cochliobolus carbonum required for production of HC-toxin, cyclo(D-prolyl-L-alanyl-D-alanyl-L-2-amino-9, 10-epoxi-8-oxodecanoyl)." Ahn J.-H., Walton J.D. Mol. Plant Microbe Interact. 10:207-214(1997) [PubMed: 9057326] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: ATCC 90305 / SB111 / 2R15.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U73650 Genomic DNA. Translation: AAC62818.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.3.1.9. 97658. 2.3.1.38. 97658. 2.3.1.39. 97658. 3.1.2.14. 97658. 4.2.1.61. 97658.
Family and domain databases
InterPro	IPR001227. Ac_transferase_reg. IPR014043. Acyl_transferase. IPR013565. DUF1729. IPR003965. Fatty_acid_synthase. IPR016452. Fatty_acid_Synthase_bsu_fun. IPR002539. MaoC_deHydtase. [Graphical view]
Gene3D	G3DSA:3.40.366.10. Ac_transferase_reg. 2 hits.
Pfam	PF00698. Acyl_transf_1. 1 hit. PF08354. DUF1729. 1 hit. PF01575. MaoC_dehydratas. 1 hit. [Graphical view]
PIRSF	PIRSF005562. FAS_yeast_beta. 1 hit.
PRINTS	PR01483. FASYNTHASE.
ProtoNet	Search...

Entry information

Entry name

TOXC_COCCA

Accession

Primary (citable) accession number: Q92215

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)