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Q92215

- TOXC_COCCA

UniProt

Q92215 - TOXC_COCCA

Protein

Putative fatty acid synthase subunit TOXC

Gene

TOXC

Organism
Cochliobolus carbonum (Bipolaris zeicola)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Essential role in the production of HC-toxin and in pathogenicity of the fungus on maize. May contribute to the synthesis of the decanoic backbone of 2-amino-9,10-epoxi-8-oxodecanoic acid. This protein contains domains similar to those of a fatty acid synthase beta subunit, namely: [acyl-carrier protein] acetyltransferase and malonyltransferase, S-acyl fatty acid synthase thioesterase, enoyl-[acyl-carrier-protein] reductase, and 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase.1 Publication

    Catalytic activityi

    Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier-protein].
    Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein].
    A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H2O.
    An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.
    Oleoyl-[acyl-carrier-protein] + H2O = [acyl-carrier-protein] + oleate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei276 – 2761For acetyltransferase activityBy similarity
    Active sitei1828 – 18281For malonyltransferase activityBy similarity

    GO - Molecular functioni

    1. [acyl-carrier-protein] S-acetyltransferase activity Source: UniProtKB-EC
    2. [acyl-carrier-protein] S-malonyltransferase activity Source: UniProtKB-EC
    3. 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity Source: UniProtKB-EC
    4. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB-EC
    5. myristoyl-[acyl-carrier-protein] hydrolase activity Source: UniProtKB-EC
    6. oleoyl-[acyl-carrier-protein] hydrolase activity Source: UniProtKB-EC
    7. palmitoyl-[acyl-carrier-protein] hydrolase activity Source: UniProtKB-EC

    GO - Biological processi

    1. fatty acid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Lyase, Oxidoreductase, Transferase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    NAD, NADP

    Enzyme and pathway databases

    UniPathwayiUPA00874.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative fatty acid synthase subunit TOXC
    Including the following 5 domains:
    3-hydroxyacyl-[acyl-carrier-protein] dehydratase (EC:4.2.1.59)
    Enoyl-[acyl-carrier-protein] reductase [NADH] (EC:1.3.1.9)
    [Acyl-carrier-protein] acetyltransferase (EC:2.3.1.38)
    [Acyl-carrier-protein] malonyltransferase (EC:2.3.1.39)
    S-acyl fatty acid synthase thioesterase (EC:3.1.2.14)
    Gene namesi
    Name:TOXC
    OrganismiCochliobolus carbonum (Bipolaris zeicola)Imported
    Taxonomic identifieri5017 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaDothideomycetesPleosporomycetidaePleosporalesPleosporineaePleosporaceaeBipolaris

    Subcellular locationi

    GO - Cellular componenti

    1. fatty acid synthase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 20802080Putative fatty acid synthase subunit TOXCPRO_0000180291Add
    BLAST

    Proteomic databases

    PRIDEiQ92215.

    Structurei

    3D structure databases

    ProteinModelPortaliQ92215.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1538 – 1667130MaoC-likeAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 468468AcetyltransferaseBy similarityAdd
    BLAST
    Regioni481 – 869389Enoyl reductaseBy similarityAdd
    BLAST
    Regioni1155 – 1644490DehydrataseBy similarityAdd
    BLAST
    Regioni1645 – 1865221Malonyl/palmitoyl transferaseBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 MaoC-like domain.Curated

    Family and domain databases

    Gene3Di3.10.129.10. 1 hit.
    3.40.366.10. 5 hits.
    InterProiIPR001227. Ac_transferase_dom.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR013565. DUF1729.
    IPR003965. Fatty_acid_synthase.
    IPR016452. Fatty_acid_Synthase_bsu_fun.
    IPR029069. HotDog_dom.
    IPR002539. MaoC_dom.
    [Graphical view]
    PfamiPF00698. Acyl_transf_1. 1 hit.
    PF08354. DUF1729. 1 hit.
    PF01575. MaoC_dehydratas. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005562. FAS_yeast_beta. 1 hit.
    PRINTSiPR01483. FASYNTHASE.
    SUPFAMiSSF52151. SSF52151. 5 hits.
    SSF54637. SSF54637. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q92215-1 [UniParc]FASTAAdd to Basket

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    MLLTAQIPES LTPFSISHGS LSVSWLLPYR LHCYATRLYR TFEATLAARS     50
    DNSEHPITLL SSVELAAHYM CYVAHETQAN TDRACTQTHD ISKLLLEDFE 100
    ATFLRSNDIH TLASALPSSD SAKDELLRCY YETCFITKHN TPLNESALLK 150
    AAREGIVSLY TTFSGQGCGG RYFDELRELF RLYPSFVGTL ISESGNLFRE 200
    LASNPSAGRL FSKGFDIMAW LHHPQTTPDT EYLISAPVSF PIIGLVQLGH 250
    YAVSCRAMGL DPGAFQRSIR GSTGHSQGIV VAAAMSAADS WEAFDRLAIS 300
    CLTVLFWIGV RSQQAAPQMS LSPAQIQDSI DHNEDVPSPM LSIIGLSRLD 350
    VQMHIDSINH YLPQSEHISI SLVNGGRHIV VSGLPSTLYR FNLLLRKIKV 400
    PDHSGQPRAT SKQKKAQFSA RFLPITVPFH SHHLVSVGSV LEEDLKNVFI 450
    GSKDLGFPVF NTYTGRDLRA EVIGNIVPAL VRMVTQYPVF WGAAVEFPGA 500
    THILEFGPGG LSGSGALTSH IKNGTGVRVI FAGLTSGSNR QVGYKQELFA 550
    RDTCHVKFAD DWSRKYAPSL VRTSNNSIVV NTKMSRLLGL PPIMVGGMTP 600
    TTAAWGFVAA TMNAGYHIEL AAGGYSDANA FENALLNIQK TTASGRGITV 650
    NLIYLSSHAV NWQIPLLRRL IIDGFRIEGI TIGGGVPSID VAKEYITTLG 700
    IKHIGFKPGP TTAIDAVIEI AQANPTFPVF LQWTGGRSGG HHSNEDFHQP 750
    ILETYDRIRQ CDNIILIAGS GFGGAADTYP YITGEWSLRY DFPPMPFDGC 800
    LLGSRVMVAK EARTSPAAKR VIVETEGLND NEWRRTYEEA AGGIITVQSE 850
    MGQPIHKIAT RGVLFWAKLD QMIFSLPKEK RIAELQKHRS WIIKGLNDDF 900
    QKPWFGRDSA DQVVELRDMT YAEVLRRMVQ LLYVKHQRRW IHSSYAVLFK 950
    AFVNRLEERF TTKTVQSYLI QDCKTIDDPY NIITVVLLQY QQAIKETILT 1000
    PDVEYFLLLC KRRGQKPVPF VPALDEDFEF FFKKDSLWQS EDLEAVVDQD 1050
    VGRTCILQGP VAAKYSVKVD EPIAEILGSI HQGHVTRLRE ERYCATLDSI 1100
    PFVEYFGGES IQLDMSSLAD GIEQSHNEQA SIYSLPSSLS MPLPAVDVWM 1150
    SLLAGKSRSW RHAIMSAGIV IQENKCVANP MRRLFAPAHG IRVQIRKPDV 1200
    PSQTEVVLEE QQESGIYEVA VRAGLNEDGE IIVEMFERRN MSDLVVSLPC 1250
    DSGTKPEYGY APIREIMEDR NERIRRFYWS IWFGKSHPIL EGSLSDSFEC 1300
    GKEKITRQHV ESFIQAINNS TRTHKNFLEP ATNVSISFAI PVAWKAIVKP 1350
    LFLNALNGDL LQLVHLSNEF RMTPGAEPLK IGEEVSTVAR INAIMNQDSG 1400
    KMVEVSAAVL RGKEIVVEII SRFLYRGAFV DFKDTFQWRD EPLMQIQLAT 1450
    SKHIAVLRTR EWFVPTQGCN IDLVGHTLTF QMRSLYKFQS KTVFRRIETH 1500
    GKVTLELAPQ KIVQVATVQY EVGICHSNTV IEFLDRYGSY SQNSVDFEDP 1550
    VSVPNNGESL VICAPSSNEA YARTSGDLNP IHVSRTFAEY AGLPGLITHG 1600
    MYCSAAIQDL VERLVADGNA GRIRQFSMSF VGMVLPNQKL EVKLEHIGMV 1650
    EGMIRLHIEA RAQETGHRVI VGEAKITQKM TTYVFTGQGS QEKGMGMDLY 1700
    NQCPAAREVW DRGDKYFLHK YGFAITTIVR DNPKQLTVHF GGRQGEAIRQ 1750
    NYINMKVETV AEDGSIQYEK LFKDVDHNTQ FYTFRSPTGL LSATQFTQPA 1800
    LSLMARASFE HLQIQGLVDG NCYYAGHSLG EFSALAAVAG IMSVESQALI 1850
    AFYRGLTMQK AVNRDESGRS NYSMCAVDPS RISATYDEEA FLTIVREIAA 1900
    ETGWLLEVVN FNVANKQYVC AGNLHALDTL AGVTDRLRLL QINASEMEEC 1950
    LHEIIRQCVQ ETKSKSTPLE LTRGIATIPL QGIDVPFHST FLRGGVRHFR 2000
    EFLHENIDKR NINPAKLIGR YIPNVTARSF QISKDYFQYV YDLTGSSQLR 2050
    DALKNWDIYE KSNGEESNGV EECSECRNSL 2080
    Length:2,080
    Mass (Da):232,268
    Last modified:February 1, 1997 - v1
    Checksum:i424D3C489EF67087
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U73650 Genomic DNA. Translation: AAC62818.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U73650 Genomic DNA. Translation: AAC62818.1 .

    3D structure databases

    ProteinModelPortali Q92215.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q92215.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00874 .

    Family and domain databases

    Gene3Di 3.10.129.10. 1 hit.
    3.40.366.10. 5 hits.
    InterProi IPR001227. Ac_transferase_dom.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR013565. DUF1729.
    IPR003965. Fatty_acid_synthase.
    IPR016452. Fatty_acid_Synthase_bsu_fun.
    IPR029069. HotDog_dom.
    IPR002539. MaoC_dom.
    [Graphical view ]
    Pfami PF00698. Acyl_transf_1. 1 hit.
    PF08354. DUF1729. 1 hit.
    PF01575. MaoC_dehydratas. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005562. FAS_yeast_beta. 1 hit.
    PRINTSi PR01483. FASYNTHASE.
    SUPFAMi SSF52151. SSF52151. 5 hits.
    SSF54637. SSF54637. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "A fatty acid synthase gene in Cochliobolus carbonum required for production of HC-toxin, cyclo(D-prolyl-L-alanyl-D-alanyl-L-2-amino-9, 10-epoxi-8-oxodecanoyl)."
      Ahn J.-H., Walton J.D.
      Mol. Plant Microbe Interact. 10:207-214(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: ATCC 90305 / SB111 / 2R15.

    Entry informationi

    Entry nameiTOXC_COCCA
    AccessioniPrimary (citable) accession number: Q92215
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3