Q92210 (PUR6_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylaminoimidazole carboxylase
EC=4.1.1.21

Alternative name(s):
AIR carboxylase
Short name=AIRC

Gene names

Name:	ADE2
ORF Names:	CaO19.5906, CaO19.13327

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Sequence similarities	In the C-terminal section; belongs to the AIR carboxylase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro adenine biosynthetic process Inferred from mutant phenotype. Source: CGD pathogenesis Inferred from mutant phenotype. Source: CGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: InterPro phosphoribosylaminoimidazole carboxylase activity Inferred from mutant phenotype. Source: CGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 568	568	Phosphoribosylaminoimidazole carboxylase		PRO_0000075021
Regions
	Domain	110 – 298	189	ATP-grasp
	Nucleotide binding	138 – 193	56	ATP By similarity
Natural variations
	Natural variant	219	1	C → W in strain: ATCC 32354 / B311.
	Natural variant	248	1	L → P in strain: ATCC 32354 / B311.
	Natural variant	332	1	L → S in strain: ATCC 32354 / B311.
	Natural variant	352	1	T → A in strain: ATCC 32354 / B311.
Experimental info
	Sequence conflict	233 – 234	2	AK → GQ in AAC49755. Ref.1
	Sequence conflict	233 – 234	2	AK → GQ in AAC49742. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q92210 [UniParc]. Last modified October 19, 2011. Version 2. Checksum: AEB63A6E0F193F44	FASTA	568	62,413
	10 20 30 40 50 60 MDSKTVGILG GGQLGRMIVE AAHRLNIKTV ILDAAKSPAK QINALDDHVD GSFTNYDSIV 70 80 90 100 110 120 KLAEKADVLT VEIEHVDVDA LIKVQEKFPK VEIYPLPETI RLIQDKYLQK NHLIKHDVAV 130 140 150 160 170 180 TESVAVETNT VDDLLHIGEK FGYPYMLKSR TLAYDGRGNF VVKDKSYCEK ALEFLKDRPL 190 200 210 220 230 240 YAEKWCPFTK ELAVMVVRSL EGEVFAYPTV ETIHENNICH LVYAPARIPD TLAKKASILA 250 260 270 280 290 300 KNAVKSFLGC GIFGVEMFLL ENNELLINEI APRPHNSGHY TIDACVTSQF EAHVRAVTGL 310 320 330 340 350 360 PMPKGFTEFS TSITNAIMLN VLGDKATPNK ELEICRRALE TPHASVYLYG KTTRPERKMG 370 380 390 400 410 420 HINVVTSSMQ DAESRLSYIL GDTTEIPKSL ATDKESPLVG IIMGSDSDLP VMAVGARILK 430 440 450 460 470 480 QFGVPFELTI VSAHRTPHRM SEYAIEAPKR GLKCIIAGAG GAAHLPGMVA AMTPLPVIGV 490 500 510 520 530 540 PVKGSTLDGV DSLHSIVQMP RGIPVATVAI NNSTNAALLA IRILGAYDSK WLTEMNQYML 550 560 NMETEVLGKA ETLEEIGYED YLTDKLKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the Candida albicans ADE2 gene and physical separation of the two functionally distinct domains of the phosphoribosylaminoimidazole carboxylase." Schmuke J.J., Davisson V.J., Bonar S.L., Gheesling-Mullis K., Dotson S.B. Yeast 13:769-776(1997) [PubMed: 9219341] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 32354 / B311.
[2]	"Sequence analysis of Candida albicans phosphoribosyl-aminoimidazole carboxylase (ADE2) gene." Tsang W.K.P., Cao B.-Y., Wang J. Yeast 13:673-676(1997) [PubMed: 9200816] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.
[3]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U69606 Genomic DNA. Translation: AAC49755.1. U75582 Genomic DNA. Translation: AAC49742.1. AACQ01000004 Genomic DNA. Translation: EAL04254.1. AACQ01000003 Genomic DNA. Translation: EAL04409.1.
RefSeq	XP_722973.1. XM_717880.1. XP_723120.1. XM_718027.1.
3D structure databases
ProteinModelPortal	Q92210.
ModBase	Search...
Protein-protein interaction databases
STRING	Q92210.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3635259. 3635363.
KEGG	cal:CaO19.13327. cal:CaO19.5906.
Organism-specific databases
CGD	CAL0003241. ADE2.
Enzyme and pathway databases
BRENDA	4.1.1.21. 1096.
Family and domain databases
InterPro	IPR016301. AIR_COase. IPR005875. AIR_COase_ATPase-su. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR000031. N5-CAIR_Mutase_PurE_dom. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.40.50.7700. AIR_carboxyl. 1 hit. G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KO	K11808.
Pfam	PF00731. AIRC. 1 hit. PF02222. ATP-grasp. 1 hit. [Graphical view]
PIRSF	PIRSF001340. AIR_carboxylase. 1 hit.
SMART	SM01001. AIRC. 1 hit. [Graphical view]
SUPFAM	SSF52255. AIR_carboxyl. 1 hit. SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR01162. PurE. 1 hit. TIGR01161. PurK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR6_CANAL

Accession

Primary (citable) accession number: Q92210
Secondary accession number(s): P78597, Q5ANJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 19, 2011
Last modified:	January 25, 2012
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program