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Q92207

- HOG1_CANAL

UniProt

Q92207 - HOG1_CANAL

Protein

Mitogen-activated protein kinase HOG1

Gene

HOG1

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 2 (29 May 2007)
      Previous versions | rss
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    Functioni

    Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes. Regulates stress-induced production and accumulation of glycerol and D-arabitol. HOG1 is also involved in virulence, morphogenesis and oxidative stress response especially through its role in chlamydospore formation, an oxygen-dependent morphogenetic program.10 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by tyrosine and threonine phosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei52 – 521ATPPROSITE-ProRule annotation
    Active sitei144 – 1441Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi29 – 379ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: CGD
    3. protein kinase activity Source: CGD
    4. protein serine/threonine kinase activity Source: CGD

    GO - Biological processi

    1. calcium-mediated signaling Source: CGD
    2. cellular response to cadmium ion Source: CGD
    3. cellular response to drug Source: CGD
    4. cellular response to heat Source: CGD
    5. cellular response to osmotic stress Source: CGD
    6. cellular response to oxidative stress Source: CGD
    7. cellular response to pH Source: CGD
    8. chlamydospore formation Source: CGD
    9. filamentous growth Source: CGD
    10. filamentous growth of a population of unicellular organisms Source: CGD
    11. filamentous growth of a population of unicellular organisms in response to biotic stimulus Source: CGD
    12. filamentous growth of a population of unicellular organisms in response to chemical stimulus Source: CGD
    13. filamentous growth of a population of unicellular organisms in response to heat Source: CGD
    14. fungal-type cell wall organization Source: CGD
    15. intracellular accumulation of glycerol Source: CGD
    16. negative regulation of filamentous growth of a population of unicellular organisms Source: CGD
    17. negative regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus Source: CGD
    18. negative regulation of filamentous growth of a population of unicellular organisms in response to heat Source: CGD
    19. pathogenesis Source: CGD
    20. polyol biosynthetic process Source: CGD
    21. positive regulation of filamentous growth of a population of unicellular organisms in response to chemical stimulus Source: CGD
    22. protein phosphorylation Source: CGD
    23. regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus Source: CGD
    24. regulation of transcription, DNA-templated Source: UniProtKB-KW
    25. response to salt stress Source: CGD
    26. stress-activated MAPK cascade Source: CGD
    27. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.24. 1096.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase HOG1 (EC:2.7.11.24)
    Short name:
    MAP kinase HOG1
    Gene namesi
    Name:HOG1
    ORF Names:CaO19.8514, CaO19.895
    OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
    Taxonomic identifieri237561 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida
    ProteomesiUP000000559: Unassembled WGS sequence

    Organism-specific databases

    CGDiCAL0002931. HOG1.

    Subcellular locationi

    Cytoplasm. Nucleus
    Note: Predominantly cytoplasmic in unstressed cells but rapidly concentrates within the nucleus in response to hyperosmotic conditions and phosphorylation.

    GO - Cellular componenti

    1. cytoplasm Source: CGD
    2. nucleus Source: CGD
    3. protein kinase CK2 complex Source: CGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 377377Mitogen-activated protein kinase HOG1PRO_0000186328Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei174 – 1741PhosphothreonineBy similarity
    Modified residuei176 – 1761PhosphotyrosineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-174 and Tyr-176, which activates the enzyme By similarity. Phosphorylated in response to oxidative and salt stress.By similarity5 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ92207.

    Interactioni

    Protein-protein interaction databases

    BioGridi1220487. 2 interactions.
    STRINGi5476.CAL0002931.

    Structurei

    3D structure databases

    ProteinModelPortaliQ92207.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini23 – 305283Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi174 – 1763TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    KOiK04441.
    OrthoDBiEOG7K3TWD.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q92207-1 [UniParc]FASTAAdd to Basket

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    MSADGEFTRT QIFGTVFEIT NRYTELNPVG MGAFGLVCSA VDRLTGQNVA    50
    VKKVMKPFST SVLAKRTYRE LKLLKHLKHE NLITLDDIFI SPLEDIYFVN 100
    ELQGTDLHRL LNSRPLEKQF IQYFTYQIMR GLKYIHSAGV IHRDLKPSNI 150
    LINENCDLKI CDFGLARLQD PQMTGYVSTR YYRAPEIMLT WQKYDTEVDL 200
    WSVGCILAEM IEGKPLFPGK DHVHQFSIIT ELLGSPPADV IDTICSENTL 250
    RFVQSLPHRD PIPFSERFAS CTHVEPEAID LLAKLLVFDP KKRISAVEGL 300
    THPYMEAYHD PTDEPVCESK FDWSFNDADL PVDTWRVMMY SEILDFHQTV 350
    GVANETEGSE QPDSQVEQNN LDSANGA 377
    Length:377
    Mass (Da):42,947
    Last modified:May 29, 2007 - v2
    Checksum:i8DB21BD497A4F52E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti298 – 2981E → Q in CAA62214. (PubMed:8824643)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X90586 Genomic DNA. Translation: CAA62214.1.
    AACQ01000018 Genomic DNA. Translation: EAL02326.1.
    AACQ01000019 Genomic DNA. Translation: EAL02199.1.
    RefSeqiXP_721016.1. XM_715923.1.
    XP_721137.1. XM_716044.1.

    Genome annotation databases

    GeneIDi3637270.
    3637393.
    KEGGical:CaO19.8514.
    cal:CaO19.895.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X90586 Genomic DNA. Translation: CAA62214.1 .
    AACQ01000018 Genomic DNA. Translation: EAL02326.1 .
    AACQ01000019 Genomic DNA. Translation: EAL02199.1 .
    RefSeqi XP_721016.1. XM_715923.1.
    XP_721137.1. XM_716044.1.

    3D structure databases

    ProteinModelPortali Q92207.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 1220487. 2 interactions.
    STRINGi 5476.CAL0002931.

    Proteomic databases

    PRIDEi Q92207.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3637270.
    3637393.
    KEGGi cal:CaO19.8514.
    cal:CaO19.895.

    Organism-specific databases

    CGDi CAL0002931. HOG1.

    Phylogenomic databases

    eggNOGi COG0515.
    KOi K04441.
    OrthoDBi EOG7K3TWD.

    Enzyme and pathway databases

    BRENDAi 2.7.11.24. 1096.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The mitogen-activated protein kinase homolog HOG1 gene controls glycerol accumulation in the pathogenic fungus Candida albicans."
      San Jose C., Monge R.A., Perez-Diaz R., Pla J., Nombela C.
      J. Bacteriol. 178:5850-5852(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: ATCC 64385 / 1001.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SC5314 / ATCC MYA-2876.
    3. "Role of the mitogen-activated protein kinase Hog1p in morphogenesis and virulence of Candida albicans."
      Alonso-Monge R., Navarro-Garcia F., Molero G., Diez-Orejas R., Gustin M.C., Pla J., Sanchez M., Nombela C.
      J. Bacteriol. 181:3058-3068(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "The Hog1 mitogen-activated protein kinase is essential in the oxidative stress response and chlamydospore formation in Candida albicans."
      Alonso-Monge R., Navarro-Garcia F., Roman E., Negredo A.I., Eisman B., Nombela C., Pla J.
      Eukaryot. Cell 2:351-361(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION.
    5. "Candida albicans response regulator gene SSK1 regulates a subset of genes whose functions are associated with cell wall biosynthesis and adaptation to oxidative stress."
      Chauhan N., Inglis D., Roman E., Pla J., Li D., Calera J.A., Calderone R.
      Eukaryot. Cell 2:1018-1024(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION.
    6. "A conserved stress-activated protein kinase regulates a core stress response in the human pathogen Candida albicans."
      Smith D.A., Nicholls S., Morgan B.A., Brown A.J.P., Quinn J.
      Mol. Biol. Cell 15:4179-4190(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
    7. "The Pbs2 MAP kinase kinase is essential for the oxidative-stress response in the fungal pathogen Candida albicans."
      Arana D.M., Nombela C., Alonso-Monge R., Pla J.
      Microbiology 151:1033-1049(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    8. "The MAP kinase Mkc1p is activated under different stress conditions in Candida albicans."
      Navarro-Garcia F., Eisman B., Fiuza S.M., Nombela C., Pla J.
      Microbiology 151:2737-2749(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION.
    9. "The MAP kinase Hog1p differentially regulates stress-induced production and accumulation of glycerol and D-arabitol in Candida albicans."
      Kayingo G., Wong B.
      Microbiology 151:2987-2999(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "The Cek1 and Hog1 mitogen-activated protein kinases play complementary roles in cell wall biogenesis and chlamydospore formation in the fungal pathogen Candida albicans."
      Eisman B., Alonso-Monge R., Roman E., Arana D.M., Nombela C., Pla J.
      Eukaryot. Cell 5:347-358(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "Role of the Hog1 stress-activated protein kinase in the global transcriptional response to stress in the fungal pathogen Candida albicans."
      Enjalbert B., Smith D.A., Cornell M.J., Alam I., Nicholls S., Brown A.J.P., Quinn J.
      Mol. Biol. Cell 17:1018-1032(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "Functional studies of the Ssk1p response regulator protein of Candida albicans as determined by phenotypic analysis of receiver domain point mutants."
      Menon V., Li D., Chauhan N., Rajnarayanan R., Dubrovska A., West A.H., Calderone R.
      Mol. Microbiol. 62:997-1013(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION.

    Entry informationi

    Entry nameiHOG1_CANAL
    AccessioniPrimary (citable) accession number: Q92207
    Secondary accession number(s): Q5AHA1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 98 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Candida albicans
      Candida albicans: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3