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Q92207

- HOG1_CANAL

UniProt

Q92207 - HOG1_CANAL

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Protein

Mitogen-activated protein kinase HOG1

Gene

HOG1

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes. Regulates stress-induced production and accumulation of glycerol and D-arabitol. HOG1 is also involved in virulence, morphogenesis and oxidative stress response especially through its role in chlamydospore formation, an oxygen-dependent morphogenetic program.10 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521ATPPROSITE-ProRule annotation
Active sitei144 – 1441Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 379ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: CGD
  3. protein kinase activity Source: CGD
  4. protein serine/threonine kinase activity Source: CGD

GO - Biological processi

  1. calcium-mediated signaling Source: CGD
  2. cellular response to cadmium ion Source: CGD
  3. cellular response to drug Source: CGD
  4. cellular response to heat Source: CGD
  5. cellular response to osmotic stress Source: CGD
  6. cellular response to oxidative stress Source: CGD
  7. cellular response to pH Source: CGD
  8. chlamydospore formation Source: CGD
  9. filamentous growth Source: CGD
  10. filamentous growth of a population of unicellular organisms Source: CGD
  11. filamentous growth of a population of unicellular organisms in response to biotic stimulus Source: CGD
  12. filamentous growth of a population of unicellular organisms in response to chemical stimulus Source: CGD
  13. filamentous growth of a population of unicellular organisms in response to heat Source: CGD
  14. fungal-type cell wall organization Source: CGD
  15. intracellular accumulation of glycerol Source: CGD
  16. negative regulation of filamentous growth of a population of unicellular organisms Source: CGD
  17. negative regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus Source: CGD
  18. negative regulation of filamentous growth of a population of unicellular organisms in response to heat Source: CGD
  19. pathogenesis Source: CGD
  20. polyol biosynthetic process Source: CGD
  21. positive regulation of filamentous growth of a population of unicellular organisms in response to chemical stimulus Source: CGD
  22. protein phosphorylation Source: CGD
  23. regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus Source: CGD
  24. regulation of transcription, DNA-templated Source: UniProtKB-KW
  25. response to salt stress Source: CGD
  26. stress-activated MAPK cascade Source: CGD
  27. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 1096.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase HOG1 (EC:2.7.11.24)
Short name:
MAP kinase HOG1
Gene namesi
Name:HOG1
ORF Names:CaO19.8514, CaO19.895
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
ProteomesiUP000000559: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0002931. HOG1.

Subcellular locationi

Cytoplasm. Nucleus
Note: Predominantly cytoplasmic in unstressed cells but rapidly concentrates within the nucleus in response to hyperosmotic conditions and phosphorylation.

GO - Cellular componenti

  1. cytoplasm Source: CGD
  2. nucleus Source: CGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 377377Mitogen-activated protein kinase HOG1PRO_0000186328Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei174 – 1741PhosphothreonineBy similarity
Modified residuei176 – 1761PhosphotyrosineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-174 and Tyr-176, which activates the enzyme (By similarity). Phosphorylated in response to oxidative and salt stress.By similarity5 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ92207.

Interactioni

Protein-protein interaction databases

BioGridi1220487. 2 interactions.
STRINGi5476.CAL0002931.

Structurei

3D structure databases

ProteinModelPortaliQ92207.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 305283Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi174 – 1763TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
InParanoidiQ92207.
KOiK04441.
OrthoDBiEOG7K3TWD.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92207-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSADGEFTRT QIFGTVFEIT NRYTELNPVG MGAFGLVCSA VDRLTGQNVA
60 70 80 90 100
VKKVMKPFST SVLAKRTYRE LKLLKHLKHE NLITLDDIFI SPLEDIYFVN
110 120 130 140 150
ELQGTDLHRL LNSRPLEKQF IQYFTYQIMR GLKYIHSAGV IHRDLKPSNI
160 170 180 190 200
LINENCDLKI CDFGLARLQD PQMTGYVSTR YYRAPEIMLT WQKYDTEVDL
210 220 230 240 250
WSVGCILAEM IEGKPLFPGK DHVHQFSIIT ELLGSPPADV IDTICSENTL
260 270 280 290 300
RFVQSLPHRD PIPFSERFAS CTHVEPEAID LLAKLLVFDP KKRISAVEGL
310 320 330 340 350
THPYMEAYHD PTDEPVCESK FDWSFNDADL PVDTWRVMMY SEILDFHQTV
360 370
GVANETEGSE QPDSQVEQNN LDSANGA
Length:377
Mass (Da):42,947
Last modified:May 29, 2007 - v2
Checksum:i8DB21BD497A4F52E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti298 – 2981E → Q in CAA62214. (PubMed:8824643)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X90586 Genomic DNA. Translation: CAA62214.1.
AACQ01000018 Genomic DNA. Translation: EAL02326.1.
AACQ01000019 Genomic DNA. Translation: EAL02199.1.
RefSeqiXP_721016.1. XM_715923.1.
XP_721137.1. XM_716044.1.

Genome annotation databases

GeneIDi3637270.
3637393.
KEGGical:CaO19.8514.
cal:CaO19.895.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X90586 Genomic DNA. Translation: CAA62214.1 .
AACQ01000018 Genomic DNA. Translation: EAL02326.1 .
AACQ01000019 Genomic DNA. Translation: EAL02199.1 .
RefSeqi XP_721016.1. XM_715923.1.
XP_721137.1. XM_716044.1.

3D structure databases

ProteinModelPortali Q92207.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 1220487. 2 interactions.
STRINGi 5476.CAL0002931.

Proteomic databases

PRIDEi Q92207.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3637270.
3637393.
KEGGi cal:CaO19.8514.
cal:CaO19.895.

Organism-specific databases

CGDi CAL0002931. HOG1.

Phylogenomic databases

eggNOGi COG0515.
InParanoidi Q92207.
KOi K04441.
OrthoDBi EOG7K3TWD.

Enzyme and pathway databases

BRENDAi 2.7.11.24. 1096.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The mitogen-activated protein kinase homolog HOG1 gene controls glycerol accumulation in the pathogenic fungus Candida albicans."
    San Jose C., Monge R.A., Perez-Diaz R., Pla J., Nombela C.
    J. Bacteriol. 178:5850-5852(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: ATCC 64385 / 1001.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.
  3. "Role of the mitogen-activated protein kinase Hog1p in morphogenesis and virulence of Candida albicans."
    Alonso-Monge R., Navarro-Garcia F., Molero G., Diez-Orejas R., Gustin M.C., Pla J., Sanchez M., Nombela C.
    J. Bacteriol. 181:3058-3068(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "The Hog1 mitogen-activated protein kinase is essential in the oxidative stress response and chlamydospore formation in Candida albicans."
    Alonso-Monge R., Navarro-Garcia F., Roman E., Negredo A.I., Eisman B., Nombela C., Pla J.
    Eukaryot. Cell 2:351-361(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION.
  5. "Candida albicans response regulator gene SSK1 regulates a subset of genes whose functions are associated with cell wall biosynthesis and adaptation to oxidative stress."
    Chauhan N., Inglis D., Roman E., Pla J., Li D., Calera J.A., Calderone R.
    Eukaryot. Cell 2:1018-1024(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION.
  6. "A conserved stress-activated protein kinase regulates a core stress response in the human pathogen Candida albicans."
    Smith D.A., Nicholls S., Morgan B.A., Brown A.J.P., Quinn J.
    Mol. Biol. Cell 15:4179-4190(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
  7. "The Pbs2 MAP kinase kinase is essential for the oxidative-stress response in the fungal pathogen Candida albicans."
    Arana D.M., Nombela C., Alonso-Monge R., Pla J.
    Microbiology 151:1033-1049(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. "The MAP kinase Mkc1p is activated under different stress conditions in Candida albicans."
    Navarro-Garcia F., Eisman B., Fiuza S.M., Nombela C., Pla J.
    Microbiology 151:2737-2749(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION.
  9. "The MAP kinase Hog1p differentially regulates stress-induced production and accumulation of glycerol and D-arabitol in Candida albicans."
    Kayingo G., Wong B.
    Microbiology 151:2987-2999(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "The Cek1 and Hog1 mitogen-activated protein kinases play complementary roles in cell wall biogenesis and chlamydospore formation in the fungal pathogen Candida albicans."
    Eisman B., Alonso-Monge R., Roman E., Arana D.M., Nombela C., Pla J.
    Eukaryot. Cell 5:347-358(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Role of the Hog1 stress-activated protein kinase in the global transcriptional response to stress in the fungal pathogen Candida albicans."
    Enjalbert B., Smith D.A., Cornell M.J., Alam I., Nicholls S., Brown A.J.P., Quinn J.
    Mol. Biol. Cell 17:1018-1032(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Functional studies of the Ssk1p response regulator protein of Candida albicans as determined by phenotypic analysis of receiver domain point mutants."
    Menon V., Li D., Chauhan N., Rajnarayanan R., Dubrovska A., West A.H., Calderone R.
    Mol. Microbiol. 62:997-1013(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION.

Entry informationi

Entry nameiHOG1_CANAL
AccessioniPrimary (citable) accession number: Q92207
Secondary accession number(s): Q5AHA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 29, 2007
Last modified: November 26, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3