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Protein

DNA-directed RNA polymerase III subunit RPC6

Gene

Polr3f

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct RNA Pol III binding to the TFIIIB-DNA complex. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity, Transcription

Enzyme and pathway databases

ReactomeiR-MMU-1834949. Cytosolic sensors of pathogen-associated DNA.
R-MMU-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-MMU-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-MMU-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC6
Short name:
RNA polymerase III subunit C6
Alternative name(s):
DNA-directed RNA polymerase III subunit F
Gene namesi
Name:Polr3f
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1924086. Polr3f.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000739732 – 316DNA-directed RNA polymerase III subunit RPC6Add BLAST315

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Cross-linki7Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ921X6.
MaxQBiQ921X6.
PaxDbiQ921X6.
PRIDEiQ921X6.

PTM databases

iPTMnetiQ921X6.
PhosphoSitePlusiQ921X6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027427.
CleanExiMM_POLR3F.
ExpressionAtlasiQ921X6. baseline and differential.
GenevisibleiQ921X6. MM.

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits. RPC3/POLR3C, RPC6/POLR3F and RPC7/POLR3G form a Pol III subcomplex. Interacts with TBP and TFIIIB90 and GTF3C4 (By similarity).By similarity

Protein-protein interaction databases

BioGridi214030. 3 interactors.
STRINGi10090.ENSMUSP00000028914.

Structurei

Secondary structure

1316
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 13Combined sources3
Helixi15 – 28Combined sources14
Helixi35 – 41Combined sources7
Helixi47 – 60Combined sources14
Beta strandi62 – 67Combined sources6
Beta strandi69 – 76Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DK8NMR-A11-78[»]
ProteinModelPortaliQ921X6.
SMRiQ921X6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ921X6.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3233. Eukaryota.
COG5111. LUCA.
GeneTreeiENSGT00390000009679.
HOGENOMiHOG000231688.
HOVERGENiHBG006817.
InParanoidiQ921X6.
KOiK03025.
OMAiATSHEVW.
OrthoDBiEOG091G0DUX.
PhylomeDBiQ921X6.
TreeFamiTF103051.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR007832. RNA_pol_Rpc34.
IPR016049. RNA_pol_Rpc34-like.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12780. PTHR12780. 1 hit.
PfamiPF05158. RNA_pol_Rpc34. 1 hit.
[Graphical view]
PIRSFiPIRSF028763. RNA_pol_Rpc34. 1 hit.
SUPFAMiSSF46785. SSF46785. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q921X6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEVKVKVQP PDADPVEIEN RIIELCHQFP HGITDQVIQN EMPHIEAQQR
60 70 80 90 100
AVAINRLLSM GQLDLLRSNT GLLYRIKDSQ NAGKMKGSDN QEKLVYQIIE
110 120 130 140 150
DAGNKGIWSR DIRYKSNLPL TEINKILKNL ESKKLIKAVK SVAASKKKVY
160 170 180 190 200
MLYNLQPDRS VTGGAWYSDQ DFESEFVEVL NQQCFKFLQS KAETARESKQ
210 220 230 240 250
NPVIQRNSSF ASSHEVWKYI CELGISKVEL SMEDIETILN TLIYDGKVEM
260 270 280 290 300
TIIAAKEGTV GSVDGHMKLY RAVNPILPPT GVVRAPCGLC PVFEDCHEGG
310
EISPSNCIYM TEWLEF
Length:316
Mass (Da):35,652
Last modified:December 1, 2001 - v1
Checksum:i0CEB63A5B3536421
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036166 mRNA. Translation: BAC29327.1.
AK076538 mRNA. Translation: BAC36385.1.
AK144679 mRNA. Translation: BAE26008.1.
BC009159 mRNA. Translation: AAH09159.1.
CCDSiCCDS16820.1.
RefSeqiNP_084039.2. NM_029763.3.
UniGeneiMm.389351.

Genome annotation databases

EnsembliENSMUST00000028914; ENSMUSP00000028914; ENSMUSG00000027427.
GeneIDi70408.
KEGGimmu:70408.
UCSCiuc008mrg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036166 mRNA. Translation: BAC29327.1.
AK076538 mRNA. Translation: BAC36385.1.
AK144679 mRNA. Translation: BAE26008.1.
BC009159 mRNA. Translation: AAH09159.1.
CCDSiCCDS16820.1.
RefSeqiNP_084039.2. NM_029763.3.
UniGeneiMm.389351.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DK8NMR-A11-78[»]
ProteinModelPortaliQ921X6.
SMRiQ921X6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214030. 3 interactors.
STRINGi10090.ENSMUSP00000028914.

PTM databases

iPTMnetiQ921X6.
PhosphoSitePlusiQ921X6.

Proteomic databases

EPDiQ921X6.
MaxQBiQ921X6.
PaxDbiQ921X6.
PRIDEiQ921X6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028914; ENSMUSP00000028914; ENSMUSG00000027427.
GeneIDi70408.
KEGGimmu:70408.
UCSCiuc008mrg.1. mouse.

Organism-specific databases

CTDi10621.
MGIiMGI:1924086. Polr3f.

Phylogenomic databases

eggNOGiKOG3233. Eukaryota.
COG5111. LUCA.
GeneTreeiENSGT00390000009679.
HOGENOMiHOG000231688.
HOVERGENiHBG006817.
InParanoidiQ921X6.
KOiK03025.
OMAiATSHEVW.
OrthoDBiEOG091G0DUX.
PhylomeDBiQ921X6.
TreeFamiTF103051.

Enzyme and pathway databases

ReactomeiR-MMU-1834949. Cytosolic sensors of pathogen-associated DNA.
R-MMU-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-MMU-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-MMU-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Miscellaneous databases

EvolutionaryTraceiQ921X6.
PROiQ921X6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027427.
CleanExiMM_POLR3F.
ExpressionAtlasiQ921X6. baseline and differential.
GenevisibleiQ921X6. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR007832. RNA_pol_Rpc34.
IPR016049. RNA_pol_Rpc34-like.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12780. PTHR12780. 1 hit.
PfamiPF05158. RNA_pol_Rpc34. 1 hit.
[Graphical view]
PIRSFiPIRSF028763. RNA_pol_Rpc34. 1 hit.
SUPFAMiSSF46785. SSF46785. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiRPC6_MOUSE
AccessioniPrimary (citable) accession number: Q921X6
Secondary accession number(s): Q544K6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.