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Protein

Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

Gene

Mgat2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes an essential step in the conversion of oligo-mannose to complex N-glycans.By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-(alpha-D-Man-(1->6))-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-(beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6))-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein].

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Source: MGI
  • carbohydrate binding Source: Ensembl

GO - Biological processi

  • oligosaccharide biosynthetic process Source: InterPro
  • protein N-linked glycosylation via asparagine Source: MGI

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-975578 Reactions specific to the complex N-glycan synthesis pathway
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT16 Glycosyltransferase Family 16

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC:2.4.1.143)
Alternative name(s):
Beta-1,2-N-acetylglucosaminyltransferase II
GlcNAc-T II
Short name:
GNT-II
Mannoside acetylglucosaminyltransferase 2
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II
Gene namesi
Name:Mgat2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2384966 Mgat2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicSequence analysis9
Transmembranei10 – 29Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST20
Topological domaini30 – 442LumenalSequence analysisAdd BLAST413

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2375203

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805181 – 442Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferaseAdd BLAST442

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi64N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi81N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ921V5
MaxQBiQ921V5
PaxDbiQ921V5
PRIDEiQ921V5

PTM databases

PhosphoSitePlusiQ921V5

Expressioni

Gene expression databases

BgeeiENSMUSG00000043998
CleanExiMM_MGAT2
GenevisibleiQ921V5 MM

Interactioni

Protein-protein interaction databases

IntActiQ921V5, 1 interactor
MINTiQ921V5
STRINGi10090.ENSMUSP00000057905

Chemistry databases

BindingDBiQ921V5

Structurei

3D structure databases

ProteinModelPortaliQ921V5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2791 Eukaryota
ENOG410XPYF LUCA
GeneTreeiENSGT00390000007341
HOGENOMiHOG000261661
HOVERGENiHBG052467
InParanoidiQ921V5
KOiK00736
OMAiRDHELCK
OrthoDBiEOG091G05U3
PhylomeDBiQ921V5
TreeFamiTF314772

Family and domain databases

InterProiView protein in InterPro
IPR007754 GlcNAc_II
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR12871 PTHR12871, 1 hit
PfamiView protein in Pfam
PF05060 MGAT2, 1 hit
SUPFAMiSSF53448 SSF53448, 2 hits

Sequencei

Sequence statusi: Complete.

Q921V5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFRIYKRKV LILTLVVAAC GFVLWSSNGR QRKSDALGPP LLDAEPVRGA
60 70 80 90 100
GHLAVSVGIR RVSNESAAPL VPAVPRPEVD NLTLRYRSLV YQLNFDQMLR
110 120 130 140 150
NVGNDGTWSP GELVLVVQVH NRPEYLRLLI DSLRKAQGIQ EVLVIFSHDF
160 170 180 190 200
WSAEINSLIS RVDFCPVLQV FFPFSIQLYP NEFPGSDPRD CPRDLKKNAA
210 220 230 240 250
LKLGCINAEY PDSFGHYREA KFSQTKHHWW WKLHFVWERV KVLQDYTGLI
260 270 280 290 300
LFLEEDHYLA PDFYHVFKKM WKLKQQECPG CDVLSLGTYT TIRSFYGIAD
310 320 330 340 350
KVDVKTWKST EHNMGLALTR DAYQKLIECT DTFCTYDDYN WDWTLQYLTL
360 370 380 390 400
ACLPKIWKVL VPQAPRIFHA GDCGMHHKKT CRPSTQSAQI ESLLNSNKQY
410 420 430 440
LFPETLVIGE KFPMAAISPP RKNGGWGDIR DHELCKSYRR LQ
Length:442
Mass (Da):51,030
Last modified:December 1, 2001 - v1
Checksum:iE32D078C4F209843
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9K → E in BAC38888 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK083370 mRNA Translation: BAC38888.1
AK152925 mRNA Translation: BAE31600.1
BC010583 mRNA Translation: AAH10583.1
BC027169 mRNA Translation: AAH27169.1
CCDSiCCDS25947.1
RefSeqiNP_666147.1, NM_146035.2
UniGeneiMm.206642
Mm.398746
Mm.483133

Genome annotation databases

EnsembliENSMUST00000060579; ENSMUSP00000057905; ENSMUSG00000043998
GeneIDi217664
KEGGimmu:217664
UCSCiuc011ymy.1 mouse

Similar proteinsi

Entry informationi

Entry nameiMGAT2_MOUSE
AccessioniPrimary (citable) accession number: Q921V5
Secondary accession number(s): Q3U6X4, Q8C3Z6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: December 1, 2001
Last modified: April 25, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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