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Protein

Torsin-1A-interacting protein 1

Gene

Tor1aip1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for nuclear membrane integrity. Induces TOR1A and TOR1B ATPase activity and is required for their location on the nuclear membrane. Binds to A- and B-type lamins. Possible role in membrane attachment and assembly of the nuclear lamina.1 Publication

Caution

GO - Molecular functioni

GO - Biological processi

  • nuclear membrane organization Source: MGI
  • positive regulation of ATPase activity Source: UniProtKB
  • protein localization to nucleus Source: MGI

Names & Taxonomyi

Protein namesi
Recommended name:
Torsin-1A-interacting protein 1
Alternative name(s):
Lamina-associated polypeptide 1B
Short name:
LAP1B
Gene namesi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:3582693 Tor1aip1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 351NuclearSequence analysisAdd BLAST351
Transmembranei352 – 372HelicalSequence analysisAdd BLAST21
Topological domaini373 – 595Perinuclear spaceSequence analysisAdd BLAST223

Keywords - Cellular componenti

Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutant mice exhibit perinatal mortality, typically dying on the last prenatal or first postnatal day. All tissues tested exhibit nuclear membrane abnormalities with membranous vesicle-appearing structures observed in the perinuclear space of neurons.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002288361 – 595Torsin-1A-interacting protein 1Add BLAST595

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei60PhosphoserineCombined sources1
Modified residuei134PhosphoserineBy similarity1
Modified residuei140PhosphoserineCombined sources1
Modified residuei151PhosphoserineCombined sources1
Modified residuei153PhosphoserineBy similarity1
Modified residuei154PhosphoserineBy similarity1
Modified residuei155PhosphoserineBy similarity1
Modified residuei235PhosphothreonineBy similarity1
Modified residuei241PhosphoserineBy similarity1
Modified residuei244PhosphoserineBy similarity1
Modified residuei255PhosphoserineBy similarity1
Cross-linki321Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei328PhosphoserineBy similarity1
Glycosylationi411N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ921T2
PeptideAtlasiQ921T2
PRIDEiQ921T2

PTM databases

iPTMnetiQ921T2
PhosphoSitePlusiQ921T2

Expressioni

Tissue specificityi

Expressed in the spinal cord and liver (at protein level).1 Publication

Developmental stagei

At E16, widely expressed with high expression levels in hippocampus and low levels in heart. In the spinal cord, expressed as early as E12 until p21, the expression levels decrease in the adulthood (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000026466
CleanExiMM_TOR1AIP1
ExpressionAtlasiQ921T2 baseline and differential
GenevisibleiQ921T2 MM

Interactioni

Subunit structurei

Interacts with ATP1B4. Interacts with TOR1A (ATP-bound). Interacts with TOR1B, TOR2A and TOR3A. Interacts with VIM.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi22897036 interactors.
DIPiDIP-56693N
IntActiQ921T2 36 interactors.
MINTiQ921T2
STRINGi10090.ENSMUSP00000095134

Structurei

3D structure databases

ProteinModelPortaliQ921T2
SMRiQ921T2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni368 – 595Interaction with TOR1ABy similarityAdd BLAST228

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili373 – 400Sequence analysisAdd BLAST28

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi22 – 129Arg-richAdd BLAST108

Sequence similaritiesi

Belongs to the TOR1AIP family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJUB Eukaryota
ENOG4111IZJ LUCA
GeneTreeiENSGT00390000012166
HOGENOMiHOG000015293
HOVERGENiHBG083152
InParanoidiQ921T2
OMAiFQNQMKQ
OrthoDBiEOG091G05ZP
TreeFamiTF329438

Family and domain databases

Gene3Di3.40.50.121901 hit
InterProiView protein in InterPro
IPR008662 Lamina-ass_polypeptide_CLAP1C
IPR038599 LAP1C-like_C_sf
PANTHERiPTHR18843 PTHR18843, 1 hit
PfamiView protein in Pfam
PF05609 LAP1C, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q921T2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGERWQAEG PGEGWAIYVT PRAPIREGRR RLDPRNGDSS DAPAYGAHPS
60 70 80 90 100
RRGRREVRFS EEPAEVYGDF EPRAAKERSP GGRRTPPEKF RPASAGEEVR
110 120 130 140 150
ESAYNLRSRP RRQRRAQEAE EMKTRRSARL EQHSQQPQLS PATSGRGLRD
160 170 180 190 200
SPSSSEDREE DEPSSRPVTS QTASKKTLRT PEASVMNEDP ISNLCRPPLR
210 220 230 240 250
SPRLDSTYQT NGNTKTNERE ATIVQQVNFF EEGETEDDLE SSYSDITIRA
260 270 280 290 300
RSSDSLESRD EATPAAGNHP DSLRGLPHNQ DFPAHENQPL LLTSGCQENP
310 320 330 340 350
QEWVDRAVRM RSRMAYNNIQ KSNFGNQSPS TSRPQSAIHH PNEPSVKIKW
360 370 380 390 400
WLLGLVAILA VGLFWFFHTP AVETTAVQEF QNQMKQLQSK YQSQNEKLWK
410 420 430 440 450
RGTTFLEKHL NSSLPRPQPA ILLLTAAQDA AEVLKCLSEQ IADAYSSFRS
460 470 480 490 500
VRAIRIDGAG KAAQDSDLVK HEVDQELTDG FKNGQNAAVV HRFESLPAGS
510 520 530 540 550
TLIFYKYCDH ENAAFKDVAL VLTVLLEEKT LEASLGLKEI EEKVRDFLKV
560 570 580 590
KFTSSSTASS YNHMDPDKLN GLWSRISHLV LPVQPENTLK AGSCL
Length:595
Mass (Da):66,781
Last modified:April 18, 2012 - v3
Checksum:i8456CF717594ED53
GO
Isoform 2 (identifier: Q921T2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     261-279: Missing.

Show »
Length:576
Mass (Da):64,845
Checksum:iEBB638C2B681D547
GO
Isoform 3 (identifier: Q921T2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     260-335: DEATPAAGNH...NQSPSTSRPQ → E

Note: No experimental confirmation available.
Show »
Length:520
Mass (Da):58,416
Checksum:i9BCAA96171EF0E5F
GO

Sequence cautioni

The sequence BAE31466 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti108S → A in BAE31466 (PubMed:16141072).Curated1
Sequence conflicti254D → N in BAE94915 (Ref. 1) Curated1
Sequence conflicti254D → N in AAH10841 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042952260 – 335DEATP…TSRPQ → E in isoform 3. 1 PublicationAdd BLAST76
Alternative sequenceiVSP_042953261 – 279Missing in isoform 2. 1 PublicationAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB251963 mRNA Translation: BAE94915.1
AC159964 Genomic DNA No translation available.
BC010841 mRNA Translation: AAH10841.1
AK152751 mRNA Translation: BAE31466.1 Different initiation.
CCDSiCCDS15387.1 [Q921T2-3]
CCDS48402.1 [Q921T2-2]
RefSeqiNP_001153490.1, NM_001160018.1 [Q921T2-2]
NP_001153491.1, NM_001160019.1
NP_659040.2, NM_144791.2 [Q921T2-3]
UniGeneiMm.211654

Genome annotation databases

EnsembliENSMUST00000027738; ENSMUSP00000027738; ENSMUSG00000026466 [Q921T2-3]
ENSMUST00000097527; ENSMUSP00000095134; ENSMUSG00000026466 [Q921T2-2]
GeneIDi208263
KEGGimmu:208263
UCSCiuc007dbu.2 mouse [Q921T2-3]
uc007dbw.2 mouse [Q921T2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTOIP1_MOUSE
AccessioniPrimary (citable) accession number: Q921T2
Secondary accession number(s): E9QLK1, Q1EQW1, Q3U7A4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: April 18, 2012
Last modified: March 28, 2018
This is version 119 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome