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Protein

Torsin-1A-interacting protein 1

Gene

Tor1aip1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for nuclear membrane integrity. Induces TOR1A and TOR1B ATPase activity and is required for their location on the nuclear membrane. Binds to A- and B-type lamins. Possible role in membrane attachment and assembly of the nuclear lamina.1 Publication

GO - Molecular functioni

GO - Biological processi

  • nuclear membrane organization Source: MGI
  • positive regulation of ATPase activity Source: UniProtKB
  • protein localization to nucleus Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Torsin-1A-interacting protein 1
Alternative name(s):
Lamina-associated polypeptide 1B
Short name:
LAP1B
Gene namesi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:3582693. Tor1aip1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 351351NuclearSequence analysisAdd
BLAST
Transmembranei352 – 37221HelicalSequence analysisAdd
BLAST
Topological domaini373 – 595223Perinuclear spaceSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • nuclear envelope Source: MGI
  • nuclear inner membrane Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutant mice exhibit perinatal mortality, typically dying on the last prenatal or first postnatal day. All tissues tested exhibit nuclear membrane abnormalities with membranous vesicle-appearing structures observed in the perinuclear space of neurons.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 595595Torsin-1A-interacting protein 1PRO_0000228836Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei60 – 601PhosphoserineCombined sources
Modified residuei134 – 1341PhosphoserineBy similarity
Modified residuei140 – 1401PhosphoserineCombined sources
Modified residuei151 – 1511PhosphoserineCombined sources
Modified residuei153 – 1531PhosphoserineBy similarity
Modified residuei154 – 1541PhosphoserineBy similarity
Modified residuei155 – 1551PhosphoserineBy similarity
Modified residuei235 – 2351PhosphothreonineBy similarity
Modified residuei241 – 2411PhosphoserineBy similarity
Modified residuei244 – 2441PhosphoserineBy similarity
Modified residuei255 – 2551PhosphoserineBy similarity
Modified residuei328 – 3281PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ921T2.
PaxDbiQ921T2.
PeptideAtlasiQ921T2.
PRIDEiQ921T2.

PTM databases

iPTMnetiQ921T2.
PhosphoSiteiQ921T2.

Expressioni

Tissue specificityi

Expressed in the spinal cord and liver (at protein level).1 Publication

Developmental stagei

At E16, widely expressed with high expression levels in hippocampus and low levels in heart. In the spinal cord, expressed as early as E12 until p21, the expression levels decrease in the adulthood (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000026466.
CleanExiMM_TOR1AIP1.
ExpressionAtlasiQ921T2. baseline and differential.
GenevisibleiQ921T2. MM.

Interactioni

Subunit structurei

Interacts with ATP1B4. Interacts with TOR1A (ATP-bound). Interacts with TOR1B, TOR2A and TOR3A. Interacts with VIM.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi228970. 35 interactions.
DIPiDIP-56693N.
IntActiQ921T2. 35 interactions.
MINTiMINT-4122627.
STRINGi10090.ENSMUSP00000095134.

Structurei

3D structure databases

ProteinModelPortaliQ921T2.
SMRiQ921T2. Positions 372-595.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni368 – 595228Interaction with TOR1ABy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili373 – 40028Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi22 – 129108Arg-richAdd
BLAST

Sequence similaritiesi

Belongs to the TOR1AIP family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJUB. Eukaryota.
ENOG4111IZJ. LUCA.
GeneTreeiENSGT00390000012166.
HOGENOMiHOG000015293.
HOVERGENiHBG083152.
InParanoidiQ921T2.
OMAiTAVQEFQ.
OrthoDBiEOG091G05ZP.
TreeFamiTF329438.

Family and domain databases

InterProiIPR008662. Lamina-ass_polypeptide_CLAP1C.
[Graphical view]
PfamiPF05609. LAP1C. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q921T2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGERWQAEG PGEGWAIYVT PRAPIREGRR RLDPRNGDSS DAPAYGAHPS
60 70 80 90 100
RRGRREVRFS EEPAEVYGDF EPRAAKERSP GGRRTPPEKF RPASAGEEVR
110 120 130 140 150
ESAYNLRSRP RRQRRAQEAE EMKTRRSARL EQHSQQPQLS PATSGRGLRD
160 170 180 190 200
SPSSSEDREE DEPSSRPVTS QTASKKTLRT PEASVMNEDP ISNLCRPPLR
210 220 230 240 250
SPRLDSTYQT NGNTKTNERE ATIVQQVNFF EEGETEDDLE SSYSDITIRA
260 270 280 290 300
RSSDSLESRD EATPAAGNHP DSLRGLPHNQ DFPAHENQPL LLTSGCQENP
310 320 330 340 350
QEWVDRAVRM RSRMAYNNIQ KSNFGNQSPS TSRPQSAIHH PNEPSVKIKW
360 370 380 390 400
WLLGLVAILA VGLFWFFHTP AVETTAVQEF QNQMKQLQSK YQSQNEKLWK
410 420 430 440 450
RGTTFLEKHL NSSLPRPQPA ILLLTAAQDA AEVLKCLSEQ IADAYSSFRS
460 470 480 490 500
VRAIRIDGAG KAAQDSDLVK HEVDQELTDG FKNGQNAAVV HRFESLPAGS
510 520 530 540 550
TLIFYKYCDH ENAAFKDVAL VLTVLLEEKT LEASLGLKEI EEKVRDFLKV
560 570 580 590
KFTSSSTASS YNHMDPDKLN GLWSRISHLV LPVQPENTLK AGSCL
Length:595
Mass (Da):66,781
Last modified:April 18, 2012 - v3
Checksum:i8456CF717594ED53
GO
Isoform 2 (identifier: Q921T2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     261-279: Missing.

Show »
Length:576
Mass (Da):64,845
Checksum:iEBB638C2B681D547
GO
Isoform 3 (identifier: Q921T2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     260-335: DEATPAAGNH...NQSPSTSRPQ → E

Note: No experimental confirmation available.
Show »
Length:520
Mass (Da):58,416
Checksum:i9BCAA96171EF0E5F
GO

Sequence cautioni

The sequence BAE31466 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti108 – 1081S → A in BAE31466 (PubMed:16141072).Curated
Sequence conflicti254 – 2541D → N in BAE94915 (Ref. 1) Curated
Sequence conflicti254 – 2541D → N in AAH10841 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei260 – 33576DEATP…TSRPQ → E in isoform 3. 1 PublicationVSP_042952Add
BLAST
Alternative sequencei261 – 27919Missing in isoform 2. 1 PublicationVSP_042953Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB251963 mRNA. Translation: BAE94915.1.
AC159964 Genomic DNA. No translation available.
BC010841 mRNA. Translation: AAH10841.1.
AK152751 mRNA. Translation: BAE31466.1. Different initiation.
CCDSiCCDS15387.1. [Q921T2-3]
CCDS48402.1. [Q921T2-2]
RefSeqiNP_001153490.1. NM_001160018.1. [Q921T2-2]
NP_001153491.1. NM_001160019.1.
NP_659040.2. NM_144791.2. [Q921T2-3]
UniGeneiMm.211654.

Genome annotation databases

EnsembliENSMUST00000027738; ENSMUSP00000027738; ENSMUSG00000026466. [Q921T2-3]
ENSMUST00000097527; ENSMUSP00000095134; ENSMUSG00000026466. [Q921T2-2]
GeneIDi208263.
KEGGimmu:208263.
UCSCiuc007dbu.2. mouse. [Q921T2-3]
uc007dbw.2. mouse. [Q921T2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB251963 mRNA. Translation: BAE94915.1.
AC159964 Genomic DNA. No translation available.
BC010841 mRNA. Translation: AAH10841.1.
AK152751 mRNA. Translation: BAE31466.1. Different initiation.
CCDSiCCDS15387.1. [Q921T2-3]
CCDS48402.1. [Q921T2-2]
RefSeqiNP_001153490.1. NM_001160018.1. [Q921T2-2]
NP_001153491.1. NM_001160019.1.
NP_659040.2. NM_144791.2. [Q921T2-3]
UniGeneiMm.211654.

3D structure databases

ProteinModelPortaliQ921T2.
SMRiQ921T2. Positions 372-595.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228970. 35 interactions.
DIPiDIP-56693N.
IntActiQ921T2. 35 interactions.
MINTiMINT-4122627.
STRINGi10090.ENSMUSP00000095134.

PTM databases

iPTMnetiQ921T2.
PhosphoSiteiQ921T2.

Proteomic databases

MaxQBiQ921T2.
PaxDbiQ921T2.
PeptideAtlasiQ921T2.
PRIDEiQ921T2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027738; ENSMUSP00000027738; ENSMUSG00000026466. [Q921T2-3]
ENSMUST00000097527; ENSMUSP00000095134; ENSMUSG00000026466. [Q921T2-2]
GeneIDi208263.
KEGGimmu:208263.
UCSCiuc007dbu.2. mouse. [Q921T2-3]
uc007dbw.2. mouse. [Q921T2-2]

Organism-specific databases

CTDi26092.
MGIiMGI:3582693. Tor1aip1.

Phylogenomic databases

eggNOGiENOG410IJUB. Eukaryota.
ENOG4111IZJ. LUCA.
GeneTreeiENSGT00390000012166.
HOGENOMiHOG000015293.
HOVERGENiHBG083152.
InParanoidiQ921T2.
OMAiTAVQEFQ.
OrthoDBiEOG091G05ZP.
TreeFamiTF329438.

Miscellaneous databases

PROiQ921T2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026466.
CleanExiMM_TOR1AIP1.
ExpressionAtlasiQ921T2. baseline and differential.
GenevisibleiQ921T2. MM.

Family and domain databases

InterProiIPR008662. Lamina-ass_polypeptide_CLAP1C.
[Graphical view]
PfamiPF05609. LAP1C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOIP1_MOUSE
AccessioniPrimary (citable) accession number: Q921T2
Secondary accession number(s): E9QLK1, Q1EQW1, Q3U7A4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: April 18, 2012
Last modified: September 7, 2016
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.