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Protein

Ras and Rab interactor 1

Gene

Rin1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ras effector protein, which may serve as an inhibitory modulator of neuronal plasticity in aversive memory formation. Can affect Ras signaling at different levels. First, by competing with RAF1 protein for binding to activated Ras. Second, by enhancing signaling from ABL1 and ABL2, which regulate cytoskeletal remodeling. Third, by activating RAB5A, possibly by functioning as a guanine nucleotide exchange factor (GEF) for RAB5A, by exchanging bound GDP for free GTP, and facilitating Ras-activated receptor endocytosis (By similarity).By similarity1 Publication

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB-KW
  • Ras GTPase binding Source: MGI

GO - Biological processi

  • associative learning Source: MGI
  • endocytosis Source: UniProtKB-KW
  • memory Source: MGI
  • negative regulation of synaptic plasticity Source: MGI
  • signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

Endocytosis

Enzyme and pathway databases

ReactomeiR-MMU-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras and Rab interactor 1
Alternative name(s):
Ras interaction/interference protein 1
Gene namesi
Name:Rin1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2385695. Rin1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytoskeleton Source: UniProtKB-SubCell
  • dendrite Source: MGI
  • neuronal cell body Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001913181 – 763Ras and Rab interactor 1Add BLAST763

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei35Phosphotyrosine; by ABL1 and ABL2Combined sources1
Modified residuei198PhosphoserineCombined sources1
Modified residuei246PhosphoserineBy similarity1
Modified residuei319PhosphoserineBy similarity1
Modified residuei323PhosphoserineBy similarity1
Modified residuei340Phosphoserine; by PKD/PRKD1By similarity1
Modified residuei598PhosphoserineBy similarity1
Modified residuei681Omega-N-methylarginineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues by ABL1 and ABL2. Phosphorylation at Ser-340 by PRKD1 induces interaction with 14-3-3 proteins (By similarity).By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

MaxQBiQ921Q7.
PaxDbiQ921Q7.
PeptideAtlasiQ921Q7.
PRIDEiQ921Q7.

PTM databases

iPTMnetiQ921Q7.
PhosphoSitePlusiQ921Q7.

Expressioni

Tissue specificityi

Highly expressed in brain. Weakly or no expressed in other tissues, except in testis, where it is expressed at intermediate level. In brain, it is mainly expressed in postnatal forebrain neurons in which it is localized in dendrites and colocalizes with Ras.1 Publication

Gene expression databases

BgeeiENSMUSG00000024883.
CleanExiMM_RIN1.
GenevisibleiQ921Q7. MM.

Interactioni

Subunit structurei

Interacts with the GTP-bound form of Ras proteins (NRAS, HRAS and KRAS). This interaction prevents the association between RAF1 and Ras. Interacts with 14-3-3 proteins YWHAB, YWHAE and YWHAZ when phosphorylated on Ser-340. Interacts with the SH3 domain of ABL1 and ABL2. Interacts with RAB5A. The interaction with Ras is probably regulated and antagonized by the interaction with 14-3-3 proteins. The interaction with 14-3-3 proteins is regulated by phosphorylation on Ser-340 (By similarity).By similarity

GO - Molecular functioni

  • Ras GTPase binding Source: MGI

Protein-protein interaction databases

BioGridi230435. 5 interactors.
DIPiDIP-46280N.
STRINGi10090.ENSMUSP00000025818.

Structurei

3D structure databases

ProteinModelPortaliQ921Q7.
SMRiQ921Q7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 151SH2Add BLAST84
Domaini445 – 587VPS9PROSITE-ProRule annotationAdd BLAST143
Domaini613 – 695Ras-associatingPROSITE-ProRule annotationAdd BLAST83

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi247 – 256Pro-rich10
Compositional biasi402 – 601Leu-richAdd BLAST200

Sequence similaritiesi

Contains 1 Ras-associating domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.Curated
Contains 1 VPS9 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiKOG2320. Eukaryota.
ENOG410ZZW5. LUCA.
GeneTreeiENSGT00530000063053.
HOGENOMiHOG000154128.
HOVERGENiHBG036105.
InParanoidiQ921Q7.
KOiK17638.
OMAiFFNPLFP.
OrthoDBiEOG091G02V2.
PhylomeDBiQ921Q7.
TreeFamiTF331067.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR000159. RA_dom.
IPR000980. SH2.
IPR003123. VPS9.
[Graphical view]
PfamiPF02204. VPS9. 1 hit.
[Graphical view]
SMARTiSM00314. RA. 1 hit.
SM00252. SH2. 1 hit.
SM00167. VPS9. 1 hit.
[Graphical view]
SUPFAMiSSF55550. SSF55550. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS51205. VPS9. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q921Q7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDPGETGAH PLGATNLNFV PGHQQKEKPS TDPLYDTPDT RGVQAGGSQQ
60 70 80 90 100
PARTVSLRER LLITRPVWLQ LRANAAAALH VLRTEPPGTF LVRKSNTRQC
110 120 130 140 150
QALCVRLPEA SGPSFVSSHY IEESTGGVSL EGSELMFQDL VQLICGYCRT
160 170 180 190 200
RAIHQAATHK ELEAISHLGM EFWSSSLNTK DQQRPSEAPP IPRLKARSPQ
210 220 230 240 250
ELDQGTGAAL CFFNPLFPGD LGPTKREKFK RSFKVRVSTE TSSPLSPPAV
260 270 280 290 300
PPPPVPVLPG TSSSQTERLP PRQLLQRESS VGYRVPGSAA SPCLPPLPSL
310 320 330 340 350
QEVDCCSPSS SEEEGSSGSP TTSPRLSRPR HRRPLLRSMS SAFCSLLAPE
360 370 380 390 400
RQVGRAATML MQNRYTAVGQ LVQDLLTQVR AGPEPRELQG IRQALSRARA
410 420 430 440 450
MLSAELGPEK LLPPERLELV LEKSLHRSVL KPLRPILAAR LRRRLSADGS
460 470 480 490 500
LGRLAEGFRL ARTQGPGAFG SHLTLSSPVE TEQVRQKLLQ LLRAYSPSAQ
510 520 530 540 550
VKWLLQACKL LYTALKSQAG ENAGADEFLP LLSLVLAQCD LPDLLLEAEY
560 570 580 590 600
MSELLEPTLL TGEGGYYLTS LSASLALLSG LSQARALPLS PAQELQRSLA
610 620 630 640 650
LWEQRRLPAT HSFQHLLRVA YQDPSTGCTS KTLAVPPGSS IATLSQLCAT
660 670 680 690 700
KFRVTQPDAF GLFLYKDQGY HRLPPEALAH RLPATGYLIY RRAERPETQG
710 720 730 740 750
AVAEKAKTGS KGPEAGAWEE ETGGLNREGK PRIAVDQEGK DQARGGHIGP
760
EEQKAEGSQA LEE
Length:763
Mass (Da):83,014
Last modified:December 1, 2001 - v1
Checksum:i0CE76F66A4773438
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC011277 mRNA. Translation: AAH11277.1.
CCDSiCCDS29447.1.
RefSeqiNP_663470.1. NM_145495.2.
UniGeneiMm.271922.

Genome annotation databases

EnsembliENSMUST00000025818; ENSMUSP00000025818; ENSMUSG00000024883.
GeneIDi225870.
KEGGimmu:225870.
UCSCiuc008gca.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC011277 mRNA. Translation: AAH11277.1.
CCDSiCCDS29447.1.
RefSeqiNP_663470.1. NM_145495.2.
UniGeneiMm.271922.

3D structure databases

ProteinModelPortaliQ921Q7.
SMRiQ921Q7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230435. 5 interactors.
DIPiDIP-46280N.
STRINGi10090.ENSMUSP00000025818.

PTM databases

iPTMnetiQ921Q7.
PhosphoSitePlusiQ921Q7.

Proteomic databases

MaxQBiQ921Q7.
PaxDbiQ921Q7.
PeptideAtlasiQ921Q7.
PRIDEiQ921Q7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025818; ENSMUSP00000025818; ENSMUSG00000024883.
GeneIDi225870.
KEGGimmu:225870.
UCSCiuc008gca.1. mouse.

Organism-specific databases

CTDi9610.
MGIiMGI:2385695. Rin1.

Phylogenomic databases

eggNOGiKOG2320. Eukaryota.
ENOG410ZZW5. LUCA.
GeneTreeiENSGT00530000063053.
HOGENOMiHOG000154128.
HOVERGENiHBG036105.
InParanoidiQ921Q7.
KOiK17638.
OMAiFFNPLFP.
OrthoDBiEOG091G02V2.
PhylomeDBiQ921Q7.
TreeFamiTF331067.

Enzyme and pathway databases

ReactomeiR-MMU-8876198. RAB GEFs exchange GTP for GDP on RABs.

Miscellaneous databases

PROiQ921Q7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024883.
CleanExiMM_RIN1.
GenevisibleiQ921Q7. MM.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR000159. RA_dom.
IPR000980. SH2.
IPR003123. VPS9.
[Graphical view]
PfamiPF02204. VPS9. 1 hit.
[Graphical view]
SMARTiSM00314. RA. 1 hit.
SM00252. SH2. 1 hit.
SM00167. VPS9. 1 hit.
[Graphical view]
SUPFAMiSSF55550. SSF55550. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS51205. VPS9. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIN1_MOUSE
AccessioniPrimary (citable) accession number: Q921Q7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.