Reviewed, UniProtKB/Swiss-Prot Q921Q3 (ALG1_MOUSE)

Names and origin

Protein names

Recommended name:
    Chitobiosyldiphosphodolichol beta-mannosyltransferase
    EC=2.4.1.142
Alternative name(s):
    GDP-mannose-dolichol diphosphochitobiose mannosyltransferase
    GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase
    Beta-1,4-mannosyltransferase
    Asparagine-linked glycosylation protein 1

Gene names

Name:

Alg1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man5 intermediate on the cytoplasmic surface of the ER By similarity.
Catalytic activity	GDP-mannose + chitobiosyldiphosphodolichol = GDP + beta-1,4-D-mannosylchitobiosyldiphosphodolichol.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 1 family.
Sequence caution	The sequence BAE26713.1 differs from that shown. Reason: Frameshift at position 462.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitobiosyldiphosphodolichol beta-mannosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q921Q3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q921Q3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     70-96: NSKPRDELLQNDRIRIVKLTDLRGLGA → T

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Chitobiosyldiphosphodolichol beta-mannosyltransferase		PRO_0000080250
Regions
	Topological domain	1 – 2	2	Cytoplasmic Potential
	Transmembrane	3 – 23	21	Signal-anchor for type II membrane protein Potential
	Topological domain	24 – 464	441	Lumenal Potential
Amino acid modifications
	Modified residue	242	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	70 – 96	27	NSKPR…RGLGA → T in isoform 2.		VSP_032516
Experimental info
	Sequence conflict	339 – 341	3	QHV → RHI in AAH11281. Ref.2
	Sequence conflict	445	1	G → R in AAH11281. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: C8E9FF9DC615E2DF	FASTA	464	52,529
	10 20 30 40 50 60 MAASCVALLV LALLLLVLLL GLWKRGRQTG RARHMVVVVL GDVGRSPRMQ YHALSLAQSG 70 80 90 100 110 120 FSVTLLGFYN SKPRDELLQN DRIRIVKLTD LRGLGAGPRI LQYGVKVVFQ AVYLLWKMMR 130 140 150 160 170 180 MDPAAYIFLQ NPPGLPAIAV CWFVGCICGS KLVIDWHNYG YSIMGLVHGP RHPIVLLAKW 190 200 210 220 230 240 YEKFFGRLSH LNLCVTNAMR EDLAENWCVR AVTLYDKPAS FFKETPLDLQ HELFMKLSHT 250 260 270 280 290 300 YSPFQSCSDP SHPDTERSAF TERDCQSGVV RRLHGRPALL VSSTSWTEDE DFSILLRALE 310 320 330 340 350 360 KFEQQALTGD SLPSLVCVIT GKGPLREHYR HLISQKHLQH VRFCTPWLEA EDYPLLLGSA 370 380 390 400 410 420 DLGVCLHMSS SGLDLPMKVV DMFGCHLPVC AVNFKCLHEL VRHGENGLVF KDAEELAAQL 430 440 450 460 QMLFSKFPDP AGKLSQFRKK LQESGQQRWD ESWQHTVLPL LTHR
	Isoform 2. Checksum: 06B8383BA017890B	FASTA	438	49,557
	10 20 30 40 50 60 MAASCVALLV LALLLLVLLL GLWKRGRQTG RARHMVVVVL GDVGRSPRMQ YHALSLAQSG 70 80 90 100 110 120 FSVTLLGFYT GPRILQYGVK VVFQAVYLLW KMMRMDPAAY IFLQNPPGLP AIAVCWFVGC 130 140 150 160 170 180 ICGSKLVIDW HNYGYSIMGL VHGPRHPIVL LAKWYEKFFG RLSHLNLCVT NAMREDLAEN 190 200 210 220 230 240 WCVRAVTLYD KPASFFKETP LDLQHELFMK LSHTYSPFQS CSDPSHPDTE RSAFTERDCQ 250 260 270 280 290 300 SGVVRRLHGR PALLVSSTSW TEDEDFSILL RALEKFEQQA LTGDSLPSLV CVITGKGPLR 310 320 330 340 350 360 EHYRHLISQK HLQHVRFCTP WLEAEDYPLL LGSADLGVCL HMSSSGLDLP MKVVDMFGCH 370 380 390 400 410 420 LPVCAVNFKC LHELVRHGEN GLVFKDAEEL AAQLQMLFSK FPDPAGKLSQ FRKKLQESGQ 430 QRWDESWQHT VLPLLTHR

References

[1]

"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Placenta.

[2]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: FVB/N. Tissue: Mammary tumor.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK145871 mRNA. Translation: BAE26713.1. Frameshift. BC011281 mRNA. Translation: AAH11281.1.
IPI	IPI00122129. IPI00653330.
RefSeq	NP_663337.2.
UniGene	Mm.34581 Mm.45780
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT33. Glycosyltransferase Family 33.
Genome annotation databases
Ensembl	ENSMUSG00000039427. Mus musculus. [Contig view]
GeneID	208211.
KEGG	mmu:208211.
Organism-specific databases
MGI	MGI:2384774. Alg1.
Phylogenomic databases
HOVERGEN	Q921Q3.
Enzyme and pathway databases
BRENDA	2.4.1.142. 244.
Gene expression databases
Bgee	Q921Q3.
GermOnline	ENSMUSG00000039427. Mus musculus.
Family and domain databases
InterPro	IPR001296. Glyco_trans_1. [Graphical view]
Pfam	PF00534. Glycos_transf_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	372220.
SOURCE	Search...

Entry information

Entry name

ALG1_MOUSE

Accession

Primary (citable) accession number: Q921Q3
Secondary accession number(s): Q3UKT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	March 18, 2008
Last modified:	April 14, 2009
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)