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Q921Q3 (ALG1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chitobiosyldiphosphodolichol beta-mannosyltransferase

EC=2.4.1.142
Alternative name(s):
Asparagine-linked glycosylation protein 1 homolog
Beta-1,4-mannosyltransferase
GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase
GDP-mannose-dolichol diphosphochitobiose mannosyltransferase
Gene names
Name:Alg1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length482 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man5 intermediate on the cytoplasmic surface of the ER By similarity.

Catalytic activity

GDP-mannose + chitobiosyldiphosphodolichol = GDP + beta-1,4-D-mannosylchitobiosyldiphosphodolichol.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity.

Sequence similarities

Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 33 subfamily.

Sequence caution

The sequence BC011281 differs from that shown. Reason: Frameshift at position 462.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q921Q3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q921Q3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     70-96: NSKPRDELLQNDRIRIVKLTDLRGLGA → T

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 482482Chitobiosyldiphosphodolichol beta-mannosyltransferase
PRO_0000080250

Regions

Topological domain1 – 22Cytoplasmic Potential
Transmembrane3 – 2321Helical; Signal-anchor for type II membrane protein; Potential
Topological domain24 – 482459Lumenal Potential

Amino acid modifications

Modified residue2421Phosphoserine By similarity

Natural variations

Alternative sequence70 – 9627NSKPR…RGLGA → T in isoform 2.
VSP_032516

Experimental info

Sequence conflict339 – 3413QHV → RHI in BC011281. Ref.2
Sequence conflict4451G → R in BC011281. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 13, 2009. Version 3.
Checksum: FA98A1062D8A53F6

FASTA48254,417
        10         20         30         40         50         60 
MAASCVALLV LALLLLVLLL GLWKRGRQTG RARHMVVVVL GDVGRSPRMQ YHALSLAQSG 

        70         80         90        100        110        120 
FSVTLLGFYN SKPRDELLQN DRIRIVKLTD LRGLGAGPRI LQYGVKVVFQ AVYLLWKMMR 

       130        140        150        160        170        180 
MDPAAYIFLQ NPPGLPAIAV CWFVGCICGS KLVIDWHNYG YSIMGLVHGP RHPIVLLAKW 

       190        200        210        220        230        240 
YEKFFGRLSH LNLCVTNAMR EDLAENWCVR AVTLYDKPAS FFKETPLDLQ HELFMKLSHT 

       250        260        270        280        290        300 
YSPFQSCSDP SHPDTERSAF TERDCQSGVV RRLHGRPALL VSSTSWTEDE DFSILLRALE 

       310        320        330        340        350        360 
KFEQQALTGD SLPSLVCVIT GKGPLREHYR HLISQKHLQH VRFCTPWLEA EDYPLLLGSA 

       370        380        390        400        410        420 
DLGVCLHMSS SGLDLPMKVV DMFGCHLPVC AVNFKCLHEL VRHGENGLVF KDAEELAAQL 

       430        440        450        460        470        480 
QMLFSKFPDP AGKLSQFRKK LQESGQQRWD ESWQHTVLPL LAHSQMTPRP HPPCGHPSCR 


GF 

« Hide

Isoform 2 [UniParc].

Checksum: 6DFDF1F48D1C938A
Show »

FASTA45651,445

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Placenta.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: FVB/N.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK145871 mRNA. Translation: BAE26713.1.
BC011281 mRNA. No translation available.
RefSeqNP_663337.2. NM_145362.2.
UniGeneMm.34581.
Mm.45780.

3D structure databases

ProteinModelPortalQ921Q3.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT33. Glycosyltransferase Family 33.

PTM databases

PhosphoSiteQ921Q3.

Proteomic databases

PaxDbQ921Q3.
PRIDEQ921Q3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000049207; ENSMUSP00000046534; ENSMUSG00000039427. [Q921Q3-2]
ENSMUST00000100196; ENSMUSP00000097770; ENSMUSG00000039427. [Q921Q3-1]
GeneID208211.
KEGGmmu:208211.
UCSCuc007yby.2. mouse. [Q921Q3-1]
uc012aba.2. mouse. [Q921Q3-2]

Organism-specific databases

CTD56052.
MGIMGI:2384774. Alg1.

Phylogenomic databases

eggNOGCOG0438.
GeneTreeENSGT00390000008647.
HOGENOMHOG000216716.
HOVERGENHBG055036.
InParanoidQ921Q3.
KOK03842.
OMADIVGYKE.
OrthoDBEOG70W3DB.
PhylomeDBQ921Q3.
TreeFamTF314121.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

BgeeQ921Q3.
GenevestigatorQ921Q3.

Family and domain databases

InterProIPR026051. ALG1-like.
[Graphical view]
PANTHERPTHR13036. PTHR13036. 1 hit.
ProtoNetSearch...

Other

ChiTaRSALG1. mouse.
NextBio372220.
PROQ921Q3.
SOURCESearch...

Entry information

Entry nameALG1_MOUSE
AccessionPrimary (citable) accession number: Q921Q3
Secondary accession number(s): Q3UKT8
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: October 13, 2009
Last modified: April 16, 2014
This is version 96 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot