Q921Q3 (ALG1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitobiosyldiphosphodolichol beta-mannosyltransferase
EC=2.4.1.142

Alternative name(s):
Asparagine-linked glycosylation protein 1 homolog
Beta-1,4-mannosyltransferase
GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase
GDP-mannose-dolichol diphosphochitobiose mannosyltransferase

Gene names

Name:

Alg1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man5 intermediate on the cytoplasmic surface of the ER By similarity.
Catalytic activity	GDP-mannose + chitobiosyldiphosphodolichol = GDP + beta-1,4-D-mannosylchitobiosyldiphosphodolichol.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 33 subfamily.
Sequence caution	The sequence BC011281 differs from that shown. Reason: Frameshift at position 462.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitobiosyldiphosphodolichol beta-mannosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q921Q3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q921Q3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     70-96: NSKPRDELLQNDRIRIVKLTDLRGLGA → T

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	Chitobiosyldiphosphodolichol beta-mannosyltransferase		PRO_0000080250
Regions
	Topological domain	1 – 2	2	Cytoplasmic Potential
	Transmembrane	3 – 23	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	24 – 482	459	Lumenal Potential
Amino acid modifications
	Modified residue	242	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	70 – 96	27	NSKPR…RGLGA → T in isoform 2.		VSP_032516
Experimental info
	Sequence conflict	339 – 341	3	QHV → RHI in BC011281. Ref.2
	Sequence conflict	445	1	G → R in BC011281. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 13, 2009. Version 3. Checksum: FA98A1062D8A53F6	FASTA	482	54,417
	10 20 30 40 50 60 MAASCVALLV LALLLLVLLL GLWKRGRQTG RARHMVVVVL GDVGRSPRMQ YHALSLAQSG 70 80 90 100 110 120 FSVTLLGFYN SKPRDELLQN DRIRIVKLTD LRGLGAGPRI LQYGVKVVFQ AVYLLWKMMR 130 140 150 160 170 180 MDPAAYIFLQ NPPGLPAIAV CWFVGCICGS KLVIDWHNYG YSIMGLVHGP RHPIVLLAKW 190 200 210 220 230 240 YEKFFGRLSH LNLCVTNAMR EDLAENWCVR AVTLYDKPAS FFKETPLDLQ HELFMKLSHT 250 260 270 280 290 300 YSPFQSCSDP SHPDTERSAF TERDCQSGVV RRLHGRPALL VSSTSWTEDE DFSILLRALE 310 320 330 340 350 360 KFEQQALTGD SLPSLVCVIT GKGPLREHYR HLISQKHLQH VRFCTPWLEA EDYPLLLGSA 370 380 390 400 410 420 DLGVCLHMSS SGLDLPMKVV DMFGCHLPVC AVNFKCLHEL VRHGENGLVF KDAEELAAQL 430 440 450 460 470 480 QMLFSKFPDP AGKLSQFRKK LQESGQQRWD ESWQHTVLPL LAHSQMTPRP HPPCGHPSCR GF
	Isoform 2 [UniParc]. Checksum: 6DFDF1F48D1C938A	FASTA	456	51,445
	10 20 30 40 50 60 MAASCVALLV LALLLLVLLL GLWKRGRQTG RARHMVVVVL GDVGRSPRMQ YHALSLAQSG 70 80 90 100 110 120 FSVTLLGFYT GPRILQYGVK VVFQAVYLLW KMMRMDPAAY IFLQNPPGLP AIAVCWFVGC 130 140 150 160 170 180 ICGSKLVIDW HNYGYSIMGL VHGPRHPIVL LAKWYEKFFG RLSHLNLCVT NAMREDLAEN 190 200 210 220 230 240 WCVRAVTLYD KPASFFKETP LDLQHELFMK LSHTYSPFQS CSDPSHPDTE RSAFTERDCQ 250 260 270 280 290 300 SGVVRRLHGR PALLVSSTSW TEDEDFSILL RALEKFEQQA LTGDSLPSLV CVITGKGPLR 310 320 330 340 350 360 EHYRHLISQK HLQHVRFCTP WLEAEDYPLL LGSADLGVCL HMSSSGLDLP MKVVDMFGCH 370 380 390 400 410 420 LPVCAVNFKC LHELVRHGEN GLVFKDAEEL AAQLQMLFSK FPDPAGKLSQ FRKKLQESGQ 430 440 450 QRWDESWQHT VLPLLAHSQM TPRPHPPCGH PSCRGF

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Placenta.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: FVB/N. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK145871 mRNA. Translation: BAE26713.1. BC011281 mRNA. No translation available.
IPI	IPI00122129. IPI00653330.
RefSeq	NP_663337.2. NM_145362.2.
UniGene	Mm.34581.
3D structure databases
ProteinModelPortal	Q921Q3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q921Q3.
Protein family/group databases
CAZy	GT33. Glycosyltransferase Family 33.
PTM databases
PhosphoSite	Q921Q3.
Proteomic databases
PRIDE	Q921Q3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000049207; ENSMUSP00000046534; ENSMUSG00000039427. ENSMUST00000100196; ENSMUSP00000097770; ENSMUSG00000039427.
GeneID	208211.
KEGG	mmu:208211.
UCSC	uc007yby.1. mouse. uc012aba.1. mouse.
Organism-specific databases
CTD	56052.
MGI	MGI:2384774. Alg1.
Phylogenomic databases
eggNOG	roNOG14410.
GeneTree	ENSGT00390000008647.
HOGENOM	HBG319770.
HOVERGEN	HBG055036.
InParanoid	Q921Q3.
OMA	LQNPPGL.
OrthoDB	EOG49078X.
PhylomeDB	Q921Q3.
Gene expression databases
ArrayExpress	Q921Q3.
Bgee	Q921Q3.
Genevestigator	Q921Q3.
GermOnline	ENSMUSG00000039427. Mus musculus.
Family and domain databases
InterPro	IPR001296. Glyco_trans_1. [Graphical view]
KO	K03842.
Pfam	PF00534. Glycos_transf_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	372220.
SOURCE	Search...

Entry information

Entry name

ALG1_MOUSE

Accession

Primary (citable) accession number: Q921Q3
Secondary accession number(s): Q3UKT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	October 13, 2009
Last modified:	December 14, 2011
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program