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Protein

Chitobiosyldiphosphodolichol beta-mannosyltransferase

Gene

Alg1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man5 intermediate on the cytoplasmic surface of the ER (By similarity).By similarity

Catalytic activityi

GDP-mannose + chitobiosyldiphosphodolichol = GDP + beta-1,4-D-mannosylchitobiosyldiphosphodolichol.

Pathwayi

GO - Molecular functioni

  1. chitobiosyldiphosphodolichol beta-mannosyltransferase activity Source: UniProtKB-EC
  2. mannosyltransferase activity Source: GO_Central

GO - Biological processi

  1. mannosylation Source: GOC
  2. protein glycosylation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_262413. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT33. Glycosyltransferase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitobiosyldiphosphodolichol beta-mannosyltransferase (EC:2.4.1.142)
Alternative name(s):
Asparagine-linked glycosylation protein 1 homolog
Beta-1,4-mannosyltransferase
GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase
GDP-mannose-dolichol diphosphochitobiose mannosyltransferase
Gene namesi
Name:Alg1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:2384774. Alg1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 22CytoplasmicSequence Analysis
Transmembranei3 – 2321Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini24 – 482459LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: GO_Central
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 482482Chitobiosyldiphosphodolichol beta-mannosyltransferasePRO_0000080250Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei242 – 2421PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ921Q3.
PaxDbiQ921Q3.
PRIDEiQ921Q3.

PTM databases

PhosphoSiteiQ921Q3.

Expressioni

Gene expression databases

BgeeiQ921Q3.
GenevestigatoriQ921Q3.

Structurei

3D structure databases

ProteinModelPortaliQ921Q3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0438.
GeneTreeiENSGT00390000008647.
HOGENOMiHOG000216716.
HOVERGENiHBG055036.
InParanoidiQ921Q3.
KOiK03842.
OMAiSIPTFHV.
OrthoDBiEOG70W3DB.
PhylomeDBiQ921Q3.
TreeFamiTF314121.

Family and domain databases

InterProiIPR026051. ALG1-like.
[Graphical view]
PANTHERiPTHR13036. PTHR13036. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q921Q3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASCVALLV LALLLLVLLL GLWKRGRQTG RARHMVVVVL GDVGRSPRMQ
60 70 80 90 100
YHALSLAQSG FSVTLLGFYN SKPRDELLQN DRIRIVKLTD LRGLGAGPRI
110 120 130 140 150
LQYGVKVVFQ AVYLLWKMMR MDPAAYIFLQ NPPGLPAIAV CWFVGCICGS
160 170 180 190 200
KLVIDWHNYG YSIMGLVHGP RHPIVLLAKW YEKFFGRLSH LNLCVTNAMR
210 220 230 240 250
EDLAENWCVR AVTLYDKPAS FFKETPLDLQ HELFMKLSHT YSPFQSCSDP
260 270 280 290 300
SHPDTERSAF TERDCQSGVV RRLHGRPALL VSSTSWTEDE DFSILLRALE
310 320 330 340 350
KFEQQALTGD SLPSLVCVIT GKGPLREHYR HLISQKHLQH VRFCTPWLEA
360 370 380 390 400
EDYPLLLGSA DLGVCLHMSS SGLDLPMKVV DMFGCHLPVC AVNFKCLHEL
410 420 430 440 450
VRHGENGLVF KDAEELAAQL QMLFSKFPDP AGKLSQFRKK LQESGQQRWD
460 470 480
ESWQHTVLPL LAHSQMTPRP HPPCGHPSCR GF
Length:482
Mass (Da):54,417
Last modified:October 13, 2009 - v3
Checksum:iFA98A1062D8A53F6
GO
Isoform 2 (identifier: Q921Q3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     70-96: NSKPRDELLQNDRIRIVKLTDLRGLGA → T

Show »
Length:456
Mass (Da):51,445
Checksum:i6DFDF1F48D1C938A
GO

Sequence cautioni

The sequence BC011281 differs from that shown. Reason: Frameshift at position 462. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti339 – 3413QHV → RHI in BC011281. (PubMed:15489334)Curated
Sequence conflicti445 – 4451G → R in BC011281. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei70 – 9627NSKPR…RGLGA → T in isoform 2. 1 PublicationVSP_032516Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK145871 mRNA. Translation: BAE26713.1.
BC011281 mRNA. No translation available.
CCDSiCCDS49753.1. [Q921Q3-1]
RefSeqiNP_663337.2. NM_145362.2. [Q921Q3-1]
UniGeneiMm.34581.
Mm.45780.

Genome annotation databases

EnsembliENSMUST00000049207; ENSMUSP00000046534; ENSMUSG00000039427. [Q921Q3-2]
ENSMUST00000100196; ENSMUSP00000097770; ENSMUSG00000039427. [Q921Q3-1]
GeneIDi208211.
KEGGimmu:208211.
UCSCiuc007yby.2. mouse. [Q921Q3-1]
uc012aba.2. mouse. [Q921Q3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK145871 mRNA. Translation: BAE26713.1.
BC011281 mRNA. No translation available.
CCDSiCCDS49753.1. [Q921Q3-1]
RefSeqiNP_663337.2. NM_145362.2. [Q921Q3-1]
UniGeneiMm.34581.
Mm.45780.

3D structure databases

ProteinModelPortaliQ921Q3.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT33. Glycosyltransferase Family 33.

PTM databases

PhosphoSiteiQ921Q3.

Proteomic databases

MaxQBiQ921Q3.
PaxDbiQ921Q3.
PRIDEiQ921Q3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049207; ENSMUSP00000046534; ENSMUSG00000039427. [Q921Q3-2]
ENSMUST00000100196; ENSMUSP00000097770; ENSMUSG00000039427. [Q921Q3-1]
GeneIDi208211.
KEGGimmu:208211.
UCSCiuc007yby.2. mouse. [Q921Q3-1]
uc012aba.2. mouse. [Q921Q3-2]

Organism-specific databases

CTDi56052.
MGIiMGI:2384774. Alg1.

Phylogenomic databases

eggNOGiCOG0438.
GeneTreeiENSGT00390000008647.
HOGENOMiHOG000216716.
HOVERGENiHBG055036.
InParanoidiQ921Q3.
KOiK03842.
OMAiSIPTFHV.
OrthoDBiEOG70W3DB.
PhylomeDBiQ921Q3.
TreeFamiTF314121.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_262413. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

ChiTaRSiAlg1. mouse.
NextBioi372220.
PROiQ921Q3.
SOURCEiSearch...

Gene expression databases

BgeeiQ921Q3.
GenevestigatoriQ921Q3.

Family and domain databases

InterProiIPR026051. ALG1-like.
[Graphical view]
PANTHERiPTHR13036. PTHR13036. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Placenta.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiALG1_MOUSE
AccessioniPrimary (citable) accession number: Q921Q3
Secondary accession number(s): Q3UKT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: October 13, 2009
Last modified: February 4, 2015
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.