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Protein

Probable ATP-dependent RNA helicase DDX27

Gene

Ddx27

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable ATP-dependent RNA helicase. Component of the nucleolar ribosomal RNA (rRNA) processing machinery that regulates 3' end formation of ribosomal 47S rRNA.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi228 – 235ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX27 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 27
Gene namesi
Name:Ddx27
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2385884. Ddx27.

Subcellular locationi

  • Nucleusnucleolus By similarity
  • Chromosome By similarity

  • Note: Associates with 60S and 90S pre-ribosomal particles.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550321 – 760Probable ATP-dependent RNA helicase DDX27Add BLAST760

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei23PhosphoserineCombined sources1
Modified residuei25PhosphoserineCombined sources1
Modified residuei48PhosphoserineBy similarity1
Modified residuei133PhosphoserineBy similarity1
Modified residuei144PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ921N6.
PaxDbiQ921N6.
PeptideAtlasiQ921N6.
PRIDEiQ921N6.

PTM databases

iPTMnetiQ921N6.
PhosphoSitePlusiQ921N6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000017999.
CleanExiMM_DDX27.
ExpressionAtlasiQ921N6. baseline and differential.
GenevisibleiQ921N6. MM.

Interactioni

Subunit structurei

Associates with PeBoW complex, composed of BOP1, PES1 and WDR12. Interacts directly with BOP1 and PES1.By similarity

Protein-protein interaction databases

BioGridi230792. 3 interactors.
IntActiQ921N6. 1 interactor.
STRINGi10090.ENSMUSP00000018143.

Structurei

3D structure databases

ProteinModelPortaliQ921N6.
SMRiQ921N6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini215 – 389Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST175
Domaini419 – 569Helicase C-terminalPROSITE-ProRule annotationAdd BLAST151

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi55 – 57Required for interaction with the PEBOW complexBy similarity3
Motifi157 – 166Nuclear localization signalSequence analysis10
Motifi184 – 212Q motifAdd BLAST29
Motifi337 – 340DEAD box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi13 – 182Asp/Glu/Lys-richAdd BLAST170

Domaini

The C-terminal domain regulates nucleolar localization.By similarity

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0338. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00550000074997.
HOGENOMiHOG000265456.
HOVERGENiHBG106162.
InParanoidiQ921N6.
KOiK13181.
OMAiNHEPERS.
OrthoDBiEOG091G048O.
TreeFamiTF314780.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q921N6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLAELGFIRT IGENDEVPVE PESDSGDEEE EGPIVLGRKQ KALQKNRSAD
60 70 80 90 100
FNPDFVFTEK EGMYDGSWAL ADVMSQLKKK RAATTLDEKI EKVRKRRKAE
110 120 130 140 150
DKEAKSGKVE EKEGQADSDL KGQENPGEDE AGSKDEDSET DYSSEDEEIL
160 170 180 190 200
TKADTLKVKE KKKKKKGQAA GGFFEDASEY DKSLSFQDMN LSRPLLKAIT
210 220 230 240 250
AMGFKQPTPI QKACIPVGLL GKDICACAAT GTGKTAAFAL PVLERLIYKP
260 270 280 290 300
RQAAVTRVLV LVPTRELGIQ VHSVTKQLAQ FCSITTCLAV GGLDVKSQEA
310 320 330 340 350
ALRAAPDILI ATPGRLIDHL HNCPSFHLSS IEVLILDEAD RMLDEYFEEQ
360 370 380 390 400
MKEIIRMCSH HRQTMLFSAT MTDEVKDLAS VSLKNPVRIF VNSNTDVAPF
410 420 430 440 450
LRQEFIRIRP NREGDREAIV AALLMRTFTD HVMLFTQTKK QAHRMHILLG
460 470 480 490 500
LLGLQVGELH GNLSQTQRLE ALRRFKDEQI DILVATDVAA RGLDIEGVKT
510 520 530 540 550
VINFTMPNTV KHYVHRVGRT ARAGRAGRSV SLVGEEERKM LKEIVKAAKA
560 570 580 590 600
PVKARILPQD VILKFRDKIE KLEKDVYAVL QLEAEEKEMQ QSEAQIDTAQ
610 620 630 640 650
RLLAKGKETA DQEPERSWFQ TKEERKKEKI AKALQEFDLA LRGKKKRKKF
660 670 680 690 700
MKDAKKKGEM TAEERSQFEI LKAQMFAERL AKRNRRTKRA RAMPEDEPTG
710 720 730 740 750
PAKKQKQQQK SVFDEELTNT SKKALKQYRA GPSFEERKQS GLPRQRRGNF
760
KSKSRYKRKK
Length:760
Mass (Da):85,939
Last modified:July 27, 2011 - v3
Checksum:iC351B13DAC1B1E0B
GO
Isoform 2 (identifier: Q921N6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     292-292: G → E
     293-760: Missing.

Note: No experimental confirmation available.
Show »
Length:292
Mass (Da):32,023
Checksum:iEA75C256EE2A0977
GO

Sequence cautioni

The sequence AAH11321 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti341R → K in AAH11321 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007073292G → E in isoform 2. 1 Publication1
Alternative sequenceiVSP_007074293 – 760Missing in isoform 2. 1 PublicationAdd BLAST468

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK134274 mRNA. Translation: BAE22077.1.
AK138442 mRNA. Translation: BAE23664.1.
AL591711 Genomic DNA. Translation: CAM17306.1.
CH466551 Genomic DNA. Translation: EDL06495.1.
BC011321 mRNA. Translation: AAH11321.1. Different initiation.
BC024730 mRNA. Translation: AAH24730.1.
BC026381 mRNA. Translation: AAH26381.1.
CCDSiCCDS38336.1. [Q921N6-1]
RefSeqiNP_694705.2. NM_153065.3. [Q921N6-1]
UniGeneiMm.295031.

Genome annotation databases

EnsembliENSMUST00000018143; ENSMUSP00000018143; ENSMUSG00000017999. [Q921N6-1]
ENSMUST00000150571; ENSMUSP00000135265; ENSMUSG00000017999. [Q921N6-2]
GeneIDi228889.
KEGGimmu:228889.
UCSCiuc008nza.1. mouse. [Q921N6-2]
uc008nzb.1. mouse. [Q921N6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK134274 mRNA. Translation: BAE22077.1.
AK138442 mRNA. Translation: BAE23664.1.
AL591711 Genomic DNA. Translation: CAM17306.1.
CH466551 Genomic DNA. Translation: EDL06495.1.
BC011321 mRNA. Translation: AAH11321.1. Different initiation.
BC024730 mRNA. Translation: AAH24730.1.
BC026381 mRNA. Translation: AAH26381.1.
CCDSiCCDS38336.1. [Q921N6-1]
RefSeqiNP_694705.2. NM_153065.3. [Q921N6-1]
UniGeneiMm.295031.

3D structure databases

ProteinModelPortaliQ921N6.
SMRiQ921N6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230792. 3 interactors.
IntActiQ921N6. 1 interactor.
STRINGi10090.ENSMUSP00000018143.

PTM databases

iPTMnetiQ921N6.
PhosphoSitePlusiQ921N6.

Proteomic databases

EPDiQ921N6.
PaxDbiQ921N6.
PeptideAtlasiQ921N6.
PRIDEiQ921N6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018143; ENSMUSP00000018143; ENSMUSG00000017999. [Q921N6-1]
ENSMUST00000150571; ENSMUSP00000135265; ENSMUSG00000017999. [Q921N6-2]
GeneIDi228889.
KEGGimmu:228889.
UCSCiuc008nza.1. mouse. [Q921N6-2]
uc008nzb.1. mouse. [Q921N6-1]

Organism-specific databases

CTDi55661.
MGIiMGI:2385884. Ddx27.

Phylogenomic databases

eggNOGiKOG0338. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00550000074997.
HOGENOMiHOG000265456.
HOVERGENiHBG106162.
InParanoidiQ921N6.
KOiK13181.
OMAiNHEPERS.
OrthoDBiEOG091G048O.
TreeFamiTF314780.

Miscellaneous databases

PROiQ921N6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000017999.
CleanExiMM_DDX27.
ExpressionAtlasiQ921N6. baseline and differential.
GenevisibleiQ921N6. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDDX27_MOUSE
AccessioniPrimary (citable) accession number: Q921N6
Secondary accession number(s): Q3UUG2, Q8R0W3, Q8R1E2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.