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Q921N6

- DDX27_MOUSE

UniProt

Q921N6 - DDX27_MOUSE

Protein

Probable ATP-dependent RNA helicase DDX27

Gene

Ddx27

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Probable ATP-dependent RNA helicase.

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi228 – 2358ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent helicase activity Source: InterPro
    3. nucleic acid binding Source: InterPro

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable ATP-dependent RNA helicase DDX27 (EC:3.6.4.13)
    Alternative name(s):
    DEAD box protein 27
    Gene namesi
    Name:Ddx27
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:2385884. Ddx27.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 760760Probable ATP-dependent RNA helicase DDX27PRO_0000055032Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei23 – 231Phosphoserine1 Publication
    Modified residuei25 – 251Phosphoserine1 Publication
    Modified residuei48 – 481PhosphoserineBy similarity
    Modified residuei133 – 1331PhosphoserineBy similarity
    Modified residuei144 – 1441PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ921N6.
    PaxDbiQ921N6.
    PRIDEiQ921N6.

    PTM databases

    PhosphoSiteiQ921N6.

    Expressioni

    Gene expression databases

    ArrayExpressiQ921N6.
    BgeeiQ921N6.
    CleanExiMM_DDX27.
    GenevestigatoriQ921N6.

    Interactioni

    Protein-protein interaction databases

    BioGridi230792. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ921N6.
    SMRiQ921N6. Positions 184-547.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini215 – 389175Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini419 – 569151Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi157 – 16610Nuclear localization signalSequence Analysis
    Motifi184 – 21229Q motifAdd
    BLAST
    Motifi337 – 3404DEAD box

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi13 – 182170Asp/Glu/Lys-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0513.
    GeneTreeiENSGT00550000074997.
    HOGENOMiHOG000265456.
    HOVERGENiHBG106162.
    InParanoidiQ3UUG2.
    KOiK13181.
    OMAiQFCNITT.
    OrthoDBiEOG7G1V5Q.
    TreeFamiTF314780.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q921N6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLAELGFIRT IGENDEVPVE PESDSGDEEE EGPIVLGRKQ KALQKNRSAD    50
    FNPDFVFTEK EGMYDGSWAL ADVMSQLKKK RAATTLDEKI EKVRKRRKAE 100
    DKEAKSGKVE EKEGQADSDL KGQENPGEDE AGSKDEDSET DYSSEDEEIL 150
    TKADTLKVKE KKKKKKGQAA GGFFEDASEY DKSLSFQDMN LSRPLLKAIT 200
    AMGFKQPTPI QKACIPVGLL GKDICACAAT GTGKTAAFAL PVLERLIYKP 250
    RQAAVTRVLV LVPTRELGIQ VHSVTKQLAQ FCSITTCLAV GGLDVKSQEA 300
    ALRAAPDILI ATPGRLIDHL HNCPSFHLSS IEVLILDEAD RMLDEYFEEQ 350
    MKEIIRMCSH HRQTMLFSAT MTDEVKDLAS VSLKNPVRIF VNSNTDVAPF 400
    LRQEFIRIRP NREGDREAIV AALLMRTFTD HVMLFTQTKK QAHRMHILLG 450
    LLGLQVGELH GNLSQTQRLE ALRRFKDEQI DILVATDVAA RGLDIEGVKT 500
    VINFTMPNTV KHYVHRVGRT ARAGRAGRSV SLVGEEERKM LKEIVKAAKA 550
    PVKARILPQD VILKFRDKIE KLEKDVYAVL QLEAEEKEMQ QSEAQIDTAQ 600
    RLLAKGKETA DQEPERSWFQ TKEERKKEKI AKALQEFDLA LRGKKKRKKF 650
    MKDAKKKGEM TAEERSQFEI LKAQMFAERL AKRNRRTKRA RAMPEDEPTG 700
    PAKKQKQQQK SVFDEELTNT SKKALKQYRA GPSFEERKQS GLPRQRRGNF 750
    KSKSRYKRKK 760
    Length:760
    Mass (Da):85,939
    Last modified:July 27, 2011 - v3
    Checksum:iC351B13DAC1B1E0B
    GO
    Isoform 2 (identifier: Q921N6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         292-292: G → E
         293-760: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:292
    Mass (Da):32,023
    Checksum:iEA75C256EE2A0977
    GO

    Sequence cautioni

    The sequence AAH11321.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti341 – 3411R → K in AAH11321. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei292 – 2921G → E in isoform 2. 1 PublicationVSP_007073
    Alternative sequencei293 – 760468Missing in isoform 2. 1 PublicationVSP_007074Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK134274 mRNA. Translation: BAE22077.1.
    AK138442 mRNA. Translation: BAE23664.1.
    AL591711 Genomic DNA. Translation: CAM17306.1.
    CH466551 Genomic DNA. Translation: EDL06495.1.
    BC011321 mRNA. Translation: AAH11321.1. Different initiation.
    BC024730 mRNA. Translation: AAH24730.1.
    BC026381 mRNA. Translation: AAH26381.1.
    CCDSiCCDS38336.1. [Q921N6-1]
    RefSeqiNP_694705.2. NM_153065.3. [Q921N6-1]
    UniGeneiMm.295031.

    Genome annotation databases

    EnsembliENSMUST00000018143; ENSMUSP00000018143; ENSMUSG00000017999. [Q921N6-1]
    ENSMUST00000150571; ENSMUSP00000135265; ENSMUSG00000017999. [Q921N6-2]
    GeneIDi228889.
    KEGGimmu:228889.
    UCSCiuc008nza.1. mouse. [Q921N6-2]
    uc008nzb.1. mouse. [Q921N6-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK134274 mRNA. Translation: BAE22077.1 .
    AK138442 mRNA. Translation: BAE23664.1 .
    AL591711 Genomic DNA. Translation: CAM17306.1 .
    CH466551 Genomic DNA. Translation: EDL06495.1 .
    BC011321 mRNA. Translation: AAH11321.1 . Different initiation.
    BC024730 mRNA. Translation: AAH24730.1 .
    BC026381 mRNA. Translation: AAH26381.1 .
    CCDSi CCDS38336.1. [Q921N6-1 ]
    RefSeqi NP_694705.2. NM_153065.3. [Q921N6-1 ]
    UniGenei Mm.295031.

    3D structure databases

    ProteinModelPortali Q921N6.
    SMRi Q921N6. Positions 184-547.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 230792. 2 interactions.

    PTM databases

    PhosphoSitei Q921N6.

    Proteomic databases

    MaxQBi Q921N6.
    PaxDbi Q921N6.
    PRIDEi Q921N6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000018143 ; ENSMUSP00000018143 ; ENSMUSG00000017999 . [Q921N6-1 ]
    ENSMUST00000150571 ; ENSMUSP00000135265 ; ENSMUSG00000017999 . [Q921N6-2 ]
    GeneIDi 228889.
    KEGGi mmu:228889.
    UCSCi uc008nza.1. mouse. [Q921N6-2 ]
    uc008nzb.1. mouse. [Q921N6-1 ]

    Organism-specific databases

    CTDi 55661.
    MGIi MGI:2385884. Ddx27.

    Phylogenomic databases

    eggNOGi COG0513.
    GeneTreei ENSGT00550000074997.
    HOGENOMi HOG000265456.
    HOVERGENi HBG106162.
    InParanoidi Q3UUG2.
    KOi K13181.
    OMAi QFCNITT.
    OrthoDBi EOG7G1V5Q.
    TreeFami TF314780.

    Miscellaneous databases

    NextBioi 379240.
    PROi Q921N6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q921N6.
    Bgeei Q921N6.
    CleanExi MM_DDX27.
    Genevestigatori Q921N6.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    PROSITEi PS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Forelimb and Hypothalamus.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Eye, Mammary cancer and Salivary gland.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23 AND SER-25, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiDDX27_MOUSE
    AccessioniPrimary (citable) accession number: Q921N6
    Secondary accession number(s): Q3UUG2, Q8R0W3, Q8R1E2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 28, 2003
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 104 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3