Q921N6 (DDX27_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ATP-dependent RNA helicase DDX27
EC=3.6.4.13

Alternative name(s):
DEAD box protein 27

Gene names

Name:

Ddx27

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	760 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable ATP-dependent RNA helicase.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subcellular location	Nucleus Potential.
Sequence similarities	Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Sequence caution	The sequence AAH11321.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q921N6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q921N6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     292-292: G → E
     293-760: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 760	760	Probable ATP-dependent RNA helicase DDX27		PRO_0000055032
Regions
	Domain	215 – 389	175	Helicase ATP-binding
	Domain	419 – 569	151	Helicase C-terminal
	Nucleotide binding	228 – 235	8	ATP By similarity
	Motif	157 – 166	10	Nuclear localization signal Potential
	Motif	184 – 212	29	Q motif
	Motif	337 – 340	4	DEAD box
	Compositional bias	13 – 182	170	Asp/Glu/Lys-rich
Amino acid modifications
	Modified residue	23	1	Phosphoserine Ref.5
	Modified residue	25	1	Phosphoserine Ref.5
	Modified residue	48	1	Phosphoserine By similarity
	Modified residue	133	1	Phosphoserine By similarity
	Modified residue	144	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	292	1	G → E in isoform 2.		VSP_007073
	Alternative sequence	293 – 760	468	Missing in isoform 2.		VSP_007074
Experimental info
	Sequence conflict	341	1	R → K in AAH11321. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 27, 2011. Version 3. Checksum: C351B13DAC1B1E0B	FASTA	760	85,939
	10 20 30 40 50 60 MLAELGFIRT IGENDEVPVE PESDSGDEEE EGPIVLGRKQ KALQKNRSAD FNPDFVFTEK 70 80 90 100 110 120 EGMYDGSWAL ADVMSQLKKK RAATTLDEKI EKVRKRRKAE DKEAKSGKVE EKEGQADSDL 130 140 150 160 170 180 KGQENPGEDE AGSKDEDSET DYSSEDEEIL TKADTLKVKE KKKKKKGQAA GGFFEDASEY 190 200 210 220 230 240 DKSLSFQDMN LSRPLLKAIT AMGFKQPTPI QKACIPVGLL GKDICACAAT GTGKTAAFAL 250 260 270 280 290 300 PVLERLIYKP RQAAVTRVLV LVPTRELGIQ VHSVTKQLAQ FCSITTCLAV GGLDVKSQEA 310 320 330 340 350 360 ALRAAPDILI ATPGRLIDHL HNCPSFHLSS IEVLILDEAD RMLDEYFEEQ MKEIIRMCSH 370 380 390 400 410 420 HRQTMLFSAT MTDEVKDLAS VSLKNPVRIF VNSNTDVAPF LRQEFIRIRP NREGDREAIV 430 440 450 460 470 480 AALLMRTFTD HVMLFTQTKK QAHRMHILLG LLGLQVGELH GNLSQTQRLE ALRRFKDEQI 490 500 510 520 530 540 DILVATDVAA RGLDIEGVKT VINFTMPNTV KHYVHRVGRT ARAGRAGRSV SLVGEEERKM 550 560 570 580 590 600 LKEIVKAAKA PVKARILPQD VILKFRDKIE KLEKDVYAVL QLEAEEKEMQ QSEAQIDTAQ 610 620 630 640 650 660 RLLAKGKETA DQEPERSWFQ TKEERKKEKI AKALQEFDLA LRGKKKRKKF MKDAKKKGEM 670 680 690 700 710 720 TAEERSQFEI LKAQMFAERL AKRNRRTKRA RAMPEDEPTG PAKKQKQQQK SVFDEELTNT 730 740 750 760 SKKALKQYRA GPSFEERKQS GLPRQRRGNF KSKSRYKRKK
	Isoform 2 [UniParc]. Checksum: EA75C256EE2A0977	FASTA	292	32,023
	10 20 30 40 50 60 MLAELGFIRT IGENDEVPVE PESDSGDEEE EGPIVLGRKQ KALQKNRSAD FNPDFVFTEK 70 80 90 100 110 120 EGMYDGSWAL ADVMSQLKKK RAATTLDEKI EKVRKRRKAE DKEAKSGKVE EKEGQADSDL 130 140 150 160 170 180 KGQENPGEDE AGSKDEDSET DYSSEDEEIL TKADTLKVKE KKKKKKGQAA GGFFEDASEY 190 200 210 220 230 240 DKSLSFQDMN LSRPLLKAIT AMGFKQPTPI QKACIPVGLL GKDICACAAT GTGKTAAFAL 250 260 270 280 290 PVLERLIYKP RQAAVTRVLV LVPTRELGIQ VHSVTKQLAQ FCSITTCLAV GE

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Forelimb and Hypothalamus.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Eye, Mammary cancer and Salivary gland.
[5]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23 AND SER-25, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK134274 mRNA. Translation: BAE22077.1. AK138442 mRNA. Translation: BAE23664.1. AL591711 Genomic DNA. Translation: CAM17306.1. CH466551 Genomic DNA. Translation: EDL06495.1. BC011321 mRNA. Translation: AAH11321.1. Different initiation. BC024730 mRNA. Translation: AAH24730.1. BC026381 mRNA. Translation: AAH26381.1.
IPI	IPI00122038. IPI00230236.
RefSeq	NP_694705.2. NM_153065.3.
UniGene	Mm.295031.
3D structure databases
ProteinModelPortal	Q921N6.
SMR	Q921N6. Positions 185-546.
ModBase	Search...
PTM databases
PhosphoSite	Q921N6.
Proteomic databases
PaxDb	Q921N6.
PRIDE	Q921N6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000018143; ENSMUSP00000018143; ENSMUSG00000017999. ENSMUST00000150571; ENSMUSP00000135265; ENSMUSG00000017999.
GeneID	228889.
KEGG	mmu:228889.
UCSC	uc008nza.1. mouse.
Organism-specific databases
CTD	55661.
MGI	MGI:2385884. Ddx27.
Phylogenomic databases
eggNOG	COG0513.
GeneTree	ENSGT00550000074997.
HOGENOM	HOG000265456.
HOVERGEN	HBG106162.
InParanoid	Q3UUG2.
KO	K13181.
OMA	TRTFTDH.
OrthoDB	EOG4NVZJV.
Gene expression databases
ArrayExpress	Q921N6.
Bgee	Q921N6.
CleanEx	MM_DDX27.
Genevestigator	Q921N6.
GermOnline	ENSMUSG00000017999. Mus musculus.
Family and domain databases
InterPro	IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR000629. RNA-helicase_DEAD-box_CS. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00039. DEAD_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	379240.
SOURCE	Search...

Entry information

Entry name

DDX27_MOUSE

Accession

Primary (citable) accession number: Q921N6
Secondary accession number(s): Q3UUG2, Q8R0W3, Q8R1E2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 91 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program