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Protein

Golgin subfamily A member 2

Gene

Golga2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis. Also plays a key role in spindle pole assembly and centrosome organization (By similarity). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (By similarity). Regulates the meiotic spindle pole assembly, probably via the same mechanism (PubMed:21552007). Also regulates the centrosome organization (By similarity). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • asymmetric cell division Source: UniProtKB
  • centrosome organization Source: UniProtKB
  • ER to Golgi vesicle-mediated transport Source: UniProtKB
  • Golgi disassembly Source: UniProtKB
  • Golgi localization Source: MGI
  • Golgi ribbon formation Source: UniProtKB
  • microtubule nucleation Source: UniProtKB
  • mitotic spindle assembly Source: UniProtKB
  • negative regulation of autophagy Source: MGI
  • negative regulation of protein binding Source: MGI
  • positive regulation of axonogenesis Source: MGI
  • positive regulation of protein glycosylation Source: MGI
  • protein glycosylation Source: UniProtKB
  • protein homotetramerization Source: UniProtKB
  • spindle assembly Source: UniProtKB
  • spindle assembly involved in meiosis Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-162658. Golgi Cisternae Pericentriolar Stack Reorganization.
R-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-6807878. COPI-mediated anterograde transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Golgin subfamily A member 2
Alternative name(s):
130 kDa cis-Golgi matrix protein
Short name:
GM130
Gene namesi
Name:Golga2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2139395. Golga2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 999999Golgin subfamily A member 2PRO_0000190055Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei18 – 181Omega-N-methylated arginineBy similarity
Modified residuei30 – 301Dimethylated arginineBy similarity
Modified residuei37 – 371PhosphoserineBy similarity
Modified residuei273 – 2731PhosphoserineBy similarity
Modified residuei438 – 4381PhosphoserineBy similarity
Modified residuei697 – 6971PhosphoserineBy similarity
Modified residuei934 – 9341PhosphoserineBy similarity
Modified residuei978 – 9781PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated at Ser-37 by CDK1 at the onset of mitosis, inhibiting the interaction with p115/USO1 and triggering Golgi disassembly. A report however suggests that Golgi disassembly is independent of phosphorylation at Ser-37. Phosphorylated at Ser-37 in prophase as the Golgi complex starts to break down, and remains phosphorylated during further breakdown and partitioning of the Golgi fragments in metaphase and anaphase. In telophase, GM130 is dephosphorylated by PP2A as the Golgi fragments start to reassemble.By similarity
Cleaved by caspases at the onset of apoptosis.By similarity
Methylation by PRMT5 is required for Golgi ribbon formation.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ921M4.
MaxQBiQ921M4.
PaxDbiQ921M4.
PRIDEiQ921M4.

PTM databases

iPTMnetiQ921M4.
PhosphoSiteiQ921M4.
SwissPalmiQ921M4.

Expressioni

Tissue specificityi

Present in oocytes during all oocyte meiotic maturation (at protein level).1 Publication

Gene expression databases

BgeeiQ921M4.
CleanExiMM_GOLGA2.
ExpressionAtlasiQ921M4. baseline and differential.
GenevisibleiQ921M4. MM.

Interactioni

Subunit structurei

Part of a larger oligomeric complex (By similarity). Interacts with p115/USO1 (By similarity). Interacts with RAB1B that has been activated by GTP-binding. Interacts with GORASP1/GRASP65. Interacts with ZFPL1. Interacts with AKAP450/AKAP9; leading to recruit AKAP450/AKAP9 to the cis-Golgi (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ921M4. 7 interactions.
MINTiMINT-4096435.
STRINGi10090.ENSMUSP00000097768.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 8686Interaction with p115/USO1By similarityAdd
BLAST
Regioni989 – 99911Interaction with GORASP1/GRASP65By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili147 – 895749Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi26 – 4924Nuclear localization signalBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi45 – 517Poly-Lys
Compositional biasi449 – 4557Poly-Pro
Compositional biasi603 – 69593Gln-richAdd
BLAST

Domaini

Extended rod-like protein with coiled-coil domains.By similarity
The nuclear localization signal (cNLS) mediates interaction with importin-alpha, recruiting importin-alpha to the Golgi membrane and liberating TPX2.By similarity

Sequence similaritiesi

Belongs to the GOLGA2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410INYK. Eukaryota.
ENOG4111MFD. LUCA.
GeneTreeiENSGT00530000062932.
HOGENOMiHOG000218631.
HOVERGENiHBG051752.
InParanoidiQ921M4.
OrthoDBiEOG71P29T.
PhylomeDBiQ921M4.
TreeFamiTF316990.

Family and domain databases

InterProiIPR024858. Golgin_A.
[Graphical view]
PfamiPF15070. GOLGA2L5. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q921M4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWPPRFPPPR PGMSEETRQS KLAAAKKKLR EYQQKNSPGV PAGAKKKKKI
60 70 80 90 100
KNGHSPERPT ASDCQSPENV PTDHIAPAPP TAATDTMFLG VTPSPDADLT
110 120 130 140 150
QSHDAGNCSN LMEETKTFSS TESLRQLSQQ LNGLVSESTS YINGEGLTSS
160 170 180 190 200
NMKELENRYQ ELAVALDSSY VTNKQLSSTI EELKQQNQDT LDQLEKEKKD
210 220 230 240 250
YQQKLAKEQG SLREQLQVHI QTIGILVSEK AELQTALAHT QQAARQKAGE
260 270 280 290 300
SEDLASRLQS SRQRVGELER TLSTVSTQQK QADRYNKDLT KERDALKLEL
310 320 330 340 350
YKNSKSNEDL RQQNSELEEK LRVLVAEKAA AQLGVEELQK KLEMSELLLQ
360 370 380 390 400
QFSSQSSAAG GNEQLQHAME ERAQLETHVS QLMESLKQLQ VERDQYAENL
410 420 430 440 450
KGESAMWQQR VQQMAEQVHT LKEEKEHRER QVQELETSLA ALRSQMEEPP
460 470 480 490 500
PPEPPAGPSE AEEQLQGEVE QLHKELERLT GQLRAQVQDN ESLSHLNREQ
510 520 530 540 550
EGRLLELERE AQRWSEQAEE RKQILESMQS DRTTISRALS QNRELKEQLA
560 570 580 590 600
ELQNGFVRLT NENMEITSAL QSEQHVKKEL ARKLGELQER LGELKETVEL
610 620 630 640 650
KSQEAQGLQE QRDQCLSHLQ QYAAAYQQHL AAYEQLTSEK EAIHKQLLLQ
660 670 680 690 700
TQLMDQLQHE EVQGKMAAEL ARQELQEAQE RLKATSQENQ QLQAQLSLLV
710 720 730 740 750
LPGEGDVDQE EEDEEVPQSS LAIPEDLDSR EAMVAFFNAA IARAEEEQAR
760 770 780 790 800
LRVQLKEQKA RCRSLSHLAA PVQSKLEKEA VVPRNVDDSA SEESNQALHV
810 820 830 840 850
AMEKLQSRFL EVMQEKVELK ERVEELEHCC IQLSGETDTI GEYIALYQNQ
860 870 880 890 900
RAVLKARHLE KEEYISRLAQ DKEEMKVKLL ELQELVLRLV NERNEWQGKF
910 920 930 940 950
LAVSQNPGDV LTPVPTGSQE FGAADQQDDL REVSLADDIE PAQGEAGVPA
960 970 980 990
PHENPTAQQI MQLLREIQNP RERPGLGSNP CIPFFYRADE NDEVKIMVV
Length:999
Mass (Da):113,278
Last modified:April 20, 2010 - v3
Checksum:i0D84161E727EA9F5
GO
Isoform 2 (identifier: Q921M4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     70-101: Missing.

Show »
Length:967
Mass (Da):110,048
Checksum:iC8AB99D83B3205A7
GO

Sequence cautioni

The sequence AAH11407.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAM15849.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei70 – 10132Missing in isoform 2. 1 PublicationVSP_039027Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL808027 Genomic DNA. Translation: CAM15845.1.
AL808027 Genomic DNA. Translation: CAM15849.1. Different initiation.
CH466542 Genomic DNA. Translation: EDL08537.1.
BC011407 mRNA. Translation: AAH11407.1. Different initiation.
CCDSiCCDS38101.1. [Q921M4-1]
RefSeqiNP_001074437.1. NM_001080968.1. [Q921M4-1]
XP_006498556.1. XM_006498493.2. [Q921M4-2]
UniGeneiMm.106376.

Genome annotation databases

EnsembliENSMUST00000113377; ENSMUSP00000109004; ENSMUSG00000002546. [Q921M4-1]
GeneIDi99412.
KEGGimmu:99412.
UCSCiuc008jew.1. mouse. [Q921M4-1]
uc008jex.1. mouse. [Q921M4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL808027 Genomic DNA. Translation: CAM15845.1.
AL808027 Genomic DNA. Translation: CAM15849.1. Different initiation.
CH466542 Genomic DNA. Translation: EDL08537.1.
BC011407 mRNA. Translation: AAH11407.1. Different initiation.
CCDSiCCDS38101.1. [Q921M4-1]
RefSeqiNP_001074437.1. NM_001080968.1. [Q921M4-1]
XP_006498556.1. XM_006498493.2. [Q921M4-2]
UniGeneiMm.106376.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ921M4. 7 interactions.
MINTiMINT-4096435.
STRINGi10090.ENSMUSP00000097768.

PTM databases

iPTMnetiQ921M4.
PhosphoSiteiQ921M4.
SwissPalmiQ921M4.

Proteomic databases

EPDiQ921M4.
MaxQBiQ921M4.
PaxDbiQ921M4.
PRIDEiQ921M4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113377; ENSMUSP00000109004; ENSMUSG00000002546. [Q921M4-1]
GeneIDi99412.
KEGGimmu:99412.
UCSCiuc008jew.1. mouse. [Q921M4-1]
uc008jex.1. mouse. [Q921M4-2]

Organism-specific databases

CTDi2801.
MGIiMGI:2139395. Golga2.

Phylogenomic databases

eggNOGiENOG410INYK. Eukaryota.
ENOG4111MFD. LUCA.
GeneTreeiENSGT00530000062932.
HOGENOMiHOG000218631.
HOVERGENiHBG051752.
InParanoidiQ921M4.
OrthoDBiEOG71P29T.
PhylomeDBiQ921M4.
TreeFamiTF316990.

Enzyme and pathway databases

ReactomeiR-MMU-162658. Golgi Cisternae Pericentriolar Stack Reorganization.
R-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-6807878. COPI-mediated anterograde transport.

Miscellaneous databases

ChiTaRSiGolga2. mouse.
NextBioi353919.
PROiQ921M4.
SOURCEiSearch...

Gene expression databases

BgeeiQ921M4.
CleanExiMM_GOLGA2.
ExpressionAtlasiQ921M4. baseline and differential.
GenevisibleiQ921M4. MM.

Family and domain databases

InterProiIPR024858. Golgin_A.
[Graphical view]
PfamiPF15070. GOLGA2L5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 16-999 (ISOFORM 2).
    Tissue: Mammary cancer.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-978, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  5. "GM130, a cis-Golgi protein, regulates meiotic spindle assembly and asymmetric division in mouse oocyte."
    Zhang C.H., Wang Z.B., Quan S., Huang X., Tong J.S., Ma J.Y., Guo L., Wei Y.C., Ouyang Y.C., Hou Y., Xing F.Q., Sun Q.Y.
    Cell Cycle 10:1861-1870(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. "GM130 regulates Golgi-derived spindle assembly by activating TPX2 and capturing microtubules."
    Wei J.H., Zhang Z.C., Wynn R.M., Seemann J.
    Cell 162:287-299(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MICROTUBULE-BINDING.

Entry informationi

Entry nameiGOGA2_MOUSE
AccessioniPrimary (citable) accession number: Q921M4
Secondary accession number(s): A2AN43, A2AN47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 3, 2003
Last sequence update: April 20, 2010
Last modified: May 11, 2016
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.