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Protein

Splicing factor 3B subunit 3

Gene

Sf3b3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72165 mRNA Splicing - Minor Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor 3B subunit 3
Alternative name(s):
Pre-mRNA-splicing factor SF3b 130 kDa subunit
Short name:
SF3b130
Spliceosome-associated protein 130
Short name:
SAP 130
Gene namesi
Name:Sf3b3
Synonyms:Kiaa0017
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1289341 Sf3b3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002767551 – 1217Splicing factor 3B subunit 3Add BLAST1217

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei156PhosphoserineBy similarity1
Modified residuei1200PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ921M3
PaxDbiQ921M3
PeptideAtlasiQ921M3
PRIDEiQ921M3

PTM databases

iPTMnetiQ921M3
PhosphoSitePlusiQ921M3
SwissPalmiQ921M3

Expressioni

Gene expression databases

BgeeiENSMUSG00000033732
CleanExiMM_SF3B3
ExpressionAtlasiQ921M3 baseline and differential
GenevisibleiQ921M3 MM

Interactioni

Subunit structurei

Identified in the spliceosome A complex; remains associated with the spliceosome throughout the splicing process. Component of the spliceosome B complex. Identified in the spliceosome C complex. Identified in the spliceosome E complex. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A and DDX42. SF3B associates with the splicing factor SF3A and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interaction between SF3B3 and SF3B1 is tighter than the interaction between SF3B3 and SF3B2. Within the SF3B complex interacts directly with SF3B1 (via HEAT domain), SF3B5 and PHF5A. The SF3B complex composed of SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6 and PHF5A interacts with U2AF2. Associates with the STAGA transcription coactivator-HAT complex. Interacts with SUPT3H. Interacts with TAF3.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei284Interaction with SF3B5By similarity1
Sitei306Interaction with SF3B5By similarity1
Sitei352Interaction with SF3B5By similarity1
Sitei429Interaction with SF3B5By similarity1
Sitei988Interaction with SF3B1By similarity1
Sitei1171Interaction with SF3B1By similarity1

Protein-protein interaction databases

BioGridi221759, 4 interactors
IntActiQ921M3, 3 interactors
MINTiQ921M3
STRINGi10090.ENSMUSP00000045073

Structurei

3D structure databases

ProteinModelPortaliQ921M3
SMRiQ921M3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni105 – 119Interaction with PHF5A, SF3B1 and SF3B5By similarityAdd BLAST15
Regioni145 – 168Interaction with PHF5A, SF3B1 and SF3B5By similarityAdd BLAST24
Regioni193 – 231Interaction with SF3B1 and SF3B5By similarityAdd BLAST39
Regioni786 – 804Interaction with SF3B1 and SF3B5By similarityAdd BLAST19
Regioni1028 – 1049Interaction with SF3B1By similarityAdd BLAST22
Regioni1100 – 1123Interaction with SF3B5By similarityAdd BLAST24

Domaini

The core of the protein consists of three beta-propeller domains.By similarity

Sequence similaritiesi

Belongs to the RSE1 family.Curated

Phylogenomic databases

eggNOGiKOG1898 Eukaryota
ENOG410XPFQ LUCA
GeneTreeiENSGT00530000063396
HOGENOMiHOG000216677
HOVERGENiHBG093942
InParanoidiQ921M3
KOiK12830
OMAiEWKAPGK
OrthoDBiEOG091G00O8
PhylomeDBiQ921M3
TreeFamiTF105685

Family and domain databases

Gene3Di2.130.10.10, 5 hits
InterProiView protein in InterPro
IPR004871 Cleavage/polyA-sp_fac_asu_C
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF03178 CPSF_A, 1 hit
SUPFAMiSSF50978 SSF50978, 3 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q921M3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFLYNLTLQR ATGISFAIHG NFSGTKQQEI VVSRGKILEL LRPDPNTGKV
60 70 80 90 100
HTLLTVEVFG VIRSLMAFRL TGGTKDYIVV GSDSGRIVIL EYQPSKNMFE
110 120 130 140 150
KIHQETFGKS GCRRIVPGQF LAVDPKGRAV MISAIEKQKL VYILNRDAAA
160 170 180 190 200
RLTISSPLEA HKANTLVYHV VGVDVGFENP MFACLEMDYE EADNDPTGEA
210 220 230 240 250
AANTQQTLTF YELDLGLNHV VRKYSEPLEE HGNFLITVPG GSDGPSGVLI
260 270 280 290 300
CSENYITYKN FGDQPDIRCP IPRRRNDLDD PERGMIFVCS ATHKTKSMFF
310 320 330 340 350
FLAQTEQGDI FKITLETDED MVTEIRLKYF DTVPVAAAMC VLKTGFLFVA
360 370 380 390 400
SEFGNHYLYQ IAHLGDDDEE PEFSSAMPLE EGDTFFFQPR PLKNLVLVDE
410 420 430 440 450
LDSLSPILFC QIADLANEDT PQLYVACGRG PRSSLRVLRH GLEVSEMAVS
460 470 480 490 500
ELPGNPNAVW TVRRHIEDEF DAYIIVSFVN ATLVLSIGET VEEVTDSGFL
510 520 530 540 550
GTTPTLSCSL LGDDALVQVY PDGIRHIRAD KRVNEWKTPG KKTIVKCAVN
560 570 580 590 600
QRQVVIALTG GELVYFEMDP SGQLNEYTER KEMSADVVCM SLANVPPGEQ
610 620 630 640 650
RSRFLAVGLV DNTVRIISLD PSDCLQPLSM QALPAQPESL CIVEMGGTEK
660 670 680 690 700
QDELGERGSI GFLYLNIGLQ NGVLLRTVLD PVTGDLSDTR TRYLGSRPVK
710 720 730 740 750
LFRVRMQGQE AVLAMSSRSW LSYSYQSRFH LTPLSYETLE FASGFASEQC
760 770 780 790 800
PEGIVAISTN TLRILALEKL GAVFNQVAFP LQYTPRKFVI HPESNNLIII
810 820 830 840 850
ETDHNAYTEA TKAQRKQQMA EEMVEAAGED ERELAAEMAA AFLNENLPES
860 870 880 890 900
IFGAPKAGNG QWASVIRVMN PIQGNTLDLV QLEQNEAAFS VAVCRFSNTG
910 920 930 940 950
EDWYVLVGVA KDLILSPRSV AGGFVYTYKL VNNGEKLEFL HKTPVEEVPA
960 970 980 990 1000
AIAPFQGRVL IGVGKLLRVY DLGKKKLLRK CENKHIANYI SGIQTIGHRV
1010 1020 1030 1040 1050
IVSDVQESFI WVRYKRNENQ LIIFADDTYP RWVTTASLLD YDTVAGADKF
1060 1070 1080 1090 1100
GNICVVRLPP NTNDEVDEDP TGNKALWDRG LLNGASQKAE VIMNYHVGET
1110 1120 1130 1140 1150
VLSLQKTTLI PGGSESLVYT TLSGGIGILV PFTSHEDHDF FQHVEMHLRS
1160 1170 1180 1190 1200
EHPPLCGRDH LSFRSYYFPV KNVIDGDLCE QFNSMEPNKQ KNVSEELDRT
1210
PPEVSKKLED IRTRYAF
Length:1,217
Mass (Da):135,550
Last modified:December 1, 2001 - v1
Checksum:iDD12A12EC8E8B23A
GO
Isoform 2 (identifier: Q921M3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     819-913: Missing.

Note: No experimental confirmation available.
Show »
Length:1,122
Mass (Da):125,308
Checksum:i5ADE870D11D99217
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti327L → I in BAE28225 (PubMed:16141072).Curated1
Sequence conflicti870N → S in BAE28225 (PubMed:16141072).Curated1
Sequence conflicti1135H → Y in BAC97845 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_022980819 – 913Missing in isoform 2. 1 PublicationAdd BLAST95

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK085705 mRNA Translation: BAC39513.1
AK088268 mRNA Translation: BAC40248.1
AK129035 Transcribed RNA Translation: BAC97845.1
AK147914 mRNA Translation: BAE28225.1
BC011412 mRNA Translation: AAH11412.1
BC031197 mRNA Translation: AAH31197.2
BC042580 mRNA Translation: AAH42580.1
CCDSiCCDS22665.1 [Q921M3-1]
RefSeqiNP_598714.1, NM_133953.2 [Q921M3-1]
XP_006530581.1, XM_006530518.3 [Q921M3-1]
UniGeneiMm.236123

Genome annotation databases

EnsembliENSMUST00000042012; ENSMUSP00000045073; ENSMUSG00000033732 [Q921M3-1]
GeneIDi101943
KEGGimmu:101943
UCSCiuc009nlb.1 mouse [Q921M3-1]
uc009nlc.1 mouse [Q921M3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSF3B3_MOUSE
AccessioniPrimary (citable) accession number: Q921M3
Secondary accession number(s): Q3UGI3
, Q6ZQL0, Q8BUD9, Q8K2J8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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