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Q921J4

- UBE2S_MOUSE

UniProt

Q921J4 - UBE2S_MOUSE

Protein

Ubiquitin-conjugating enzyme E2 S

Gene

Ube2s

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei95 – 951Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. activation of anaphase-promoting complex activity Source: UniProtKB
    2. anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
    3. exit from mitosis Source: UniProtKB
    4. free ubiquitin chain polymerization Source: UniProtKB
    5. protein K11-linked ubiquitination Source: UniProtKB
    6. protein K27-linked ubiquitination Source: UniProtKB
    7. protein K29-linked ubiquitination Source: UniProtKB
    8. protein K63-linked ubiquitination Source: UniProtKB
    9. protein K6-linked ubiquitination Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Cell cycle, Cell division, Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 S (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein S
    Ubiquitin-conjugating enzyme E2-24 kDa
    Ubiquitin-conjugating enzyme E2-EPF5
    Ubiquitin-protein ligase S
    Gene namesi
    Name:Ube2s
    Synonyms:E2epf
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1925141. Ube2s.

    Subcellular locationi

    GO - Cellular componenti

    1. anaphase-promoting complex Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 223223Ubiquitin-conjugating enzyme E2 SPRO_0000082508Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Post-translational modificationi

    Autoubiquitinated by the APC/C complex during G1, leading to its degradation by the proteasome.By similarity

    Keywords - PTMi

    Acetylation, Ubl conjugation

    Proteomic databases

    MaxQBiQ921J4.
    PaxDbiQ921J4.
    PRIDEiQ921J4.

    2D gel databases

    REPRODUCTION-2DPAGEQ921J4.

    PTM databases

    PhosphoSiteiQ921J4.

    Expressioni

    Gene expression databases

    BgeeiQ921J4.
    CleanExiMM_UBE2S.
    GenevestigatoriQ921J4.

    Interactioni

    Subunit structurei

    Component of the APC/C complex. Interacts with CDC20, FZR1/CDH1 and VHL By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ921J4. 1 interaction.
    MINTiMINT-8178472.

    Structurei

    3D structure databases

    ProteinModelPortaliQ921J4.
    SMRiQ921J4. Positions 6-156.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00730000110565.
    HOGENOMiHOG000233455.
    HOVERGENiHBG063308.
    InParanoidiQ8CF67.
    KOiK10583.
    OMAiPDLGIKH.
    OrthoDBiEOG73JKXD.
    PhylomeDBiQ921J4.
    TreeFamiTF101120.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q921J4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSNVENLPP HIIRLVYKEV TTLTADPPDG IKVFPNEEDL TDLQVTIEGP    50
    EGTPYAGGLF RMKLLLGKDF PASPPKGYFL TKIFHPNVGP NGEICVNVLK 100
    RDWTAELGIR HVLLTIKCLL IHPNPESALN EEAGRLLLEN YEEYAARARL 150
    LTEIHGGACS TSSGRAEATQ DLASGASASS ADPMIPGVLG GAEGPMAKKH 200
    AGERDKKLAA KKKLDKKRAL RRL 223
    Length:223
    Mass (Da):24,183
    Last modified:December 1, 2001 - v1
    Checksum:i10816CE4377BC406
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti213 – 2131K → N in BAC25019. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK003078 mRNA. Translation: BAC25019.1.
    AK017648 mRNA. Translation: BAC25523.1.
    AK146276 mRNA. Translation: BAE27035.1.
    AK154026 mRNA. Translation: BAE32323.1.
    AK154067 mRNA. Translation: BAE32353.1.
    BC012255 mRNA. Translation: AAH12255.1.
    BC030171 mRNA. Translation: AAH30171.1.
    BC083323 mRNA. Translation: AAH83323.1.
    CCDSiCCDS39743.1.
    RefSeqiNP_598538.1. NM_133777.2.
    UniGeneiMm.389787.

    Genome annotation databases

    EnsembliENSMUST00000079496; ENSMUSP00000078459; ENSMUSG00000060860.
    GeneIDi77891.
    KEGGimmu:77891.
    UCSCiuc009eyt.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK003078 mRNA. Translation: BAC25019.1 .
    AK017648 mRNA. Translation: BAC25523.1 .
    AK146276 mRNA. Translation: BAE27035.1 .
    AK154026 mRNA. Translation: BAE32323.1 .
    AK154067 mRNA. Translation: BAE32353.1 .
    BC012255 mRNA. Translation: AAH12255.1 .
    BC030171 mRNA. Translation: AAH30171.1 .
    BC083323 mRNA. Translation: AAH83323.1 .
    CCDSi CCDS39743.1.
    RefSeqi NP_598538.1. NM_133777.2.
    UniGenei Mm.389787.

    3D structure databases

    ProteinModelPortali Q921J4.
    SMRi Q921J4. Positions 6-156.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q921J4. 1 interaction.
    MINTi MINT-8178472.

    PTM databases

    PhosphoSitei Q921J4.

    2D gel databases

    REPRODUCTION-2DPAGE Q921J4.

    Proteomic databases

    MaxQBi Q921J4.
    PaxDbi Q921J4.
    PRIDEi Q921J4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000079496 ; ENSMUSP00000078459 ; ENSMUSG00000060860 .
    GeneIDi 77891.
    KEGGi mmu:77891.
    UCSCi uc009eyt.1. mouse.

    Organism-specific databases

    CTDi 27338.
    MGIi MGI:1925141. Ube2s.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00730000110565.
    HOGENOMi HOG000233455.
    HOVERGENi HBG063308.
    InParanoidi Q8CF67.
    KOi K10583.
    OMAi PDLGIKH.
    OrthoDBi EOG73JKXD.
    PhylomeDBi Q921J4.
    TreeFami TF101120.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    NextBioi 347761.
    PROi Q921J4.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q921J4.
    CleanExi MM_UBE2S.
    Genevestigatori Q921J4.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J, DBA/2 and NOD.
      Tissue: Spleen and Thymus.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6 and Czech II.
      Tissue: Mammary gland.

    Entry informationi

    Entry nameiUBE2S_MOUSE
    AccessioniPrimary (citable) accession number: Q921J4
    Secondary accession number(s): Q3U4V7, Q8CF67
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 10, 2002
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3