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Q921J2

- RHEB_MOUSE

UniProt

Q921J2 - RHEB_MOUSE

Protein

GTP-binding protein Rheb

Gene

Rheb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity By similarity.By similarity

    Enzyme regulationi

    Alternates between an inactive form bound to GDP and an active form bound to GTP. Inactivated by TSC1-TSC2 via the GTPase activating protein (GAP) domain of TSC2 By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi20 – 201MagnesiumBy similarity
    Metal bindingi38 – 381MagnesiumBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi16 – 216GTPBy similarity
    Nucleotide bindingi32 – 387GTPBy similarity
    Nucleotide bindingi149 – 1502GTPBy similarity

    GO - Molecular functioni

    1. GDP binding Source: Ensembl
    2. GTP binding Source: UniProtKB
    3. metal ion binding Source: UniProtKB-KW
    4. protein kinase binding Source: UniProtKB

    GO - Biological processi

    1. GTP catabolic process Source: InterPro
    2. positive regulation of TOR signaling Source: UniProtKB
    3. small GTPase mediated signal transduction Source: InterPro

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTP-binding protein Rheb
    Alternative name(s):
    Ras homolog enriched in brain
    Gene namesi
    Name:Rheb
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:97912. Rheb.

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

    GO - Cellular componenti

    1. dendrite Source: Ensembl
    2. neuronal cell body Source: Ensembl
    3. plasma membrane Source: UniProtKB-SubCell
    4. spliceosomal complex Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi130 – 1301S → A: Abolishes phosphorylation by MAPKAPK5 and impairs GTP-binding. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 181181GTP-binding protein RhebPRO_0000082709Add
    BLAST
    Propeptidei182 – 1843Removed in mature formBy similarityPRO_0000281366

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei130 – 1301Phosphoserine; by MAPKAPK51 Publication
    Modified residuei181 – 1811Cysteine methyl esterBy similarity
    Lipidationi181 – 1811S-farnesyl cysteineBy similarity

    Post-translational modificationi

    Farnesylation is important for efficiently activating mTORC1-mediated signaling.By similarity
    Phosphorylation by MAPKAPK5 impairs GTP-binding and inactivation.1 Publication

    Keywords - PTMi

    Lipoprotein, Methylation, Phosphoprotein, Prenylation

    Proteomic databases

    MaxQBiQ921J2.
    PaxDbiQ921J2.
    PRIDEiQ921J2.

    PTM databases

    PhosphoSiteiQ921J2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ921J2.
    BgeeiQ921J2.
    CleanExiMM_RHEB.
    GenevestigatoriQ921J2.

    Interactioni

    Subunit structurei

    Binds to mTORC1 in a guanyl nucleotide-independent manner. Interacts directly with MTOR, MLST8 and RPTOR. Interacts with TSC2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi202889. 5 interactions.
    IntActiQ921J2. 2 interactions.
    MINTiMINT-4611176.

    Structurei

    Secondary structure

    1
    184
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 1410
    Helixi19 – 2810
    Beta strandi41 – 499
    Beta strandi52 – 609
    Beta strandi80 – 867
    Helixi90 – 10718
    Beta strandi114 – 1196
    Helixi124 – 1263
    Helixi131 – 14010
    Beta strandi145 – 1473
    Helixi153 – 16715

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4O25X-ray2.20A/B1-169[»]
    4O2LX-ray2.40A/B1-169[»]
    4O2RX-ray2.25A/B1-169[»]
    ProteinModelPortaliQ921J2.
    SMRiQ921J2. Positions 3-169.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi35 – 439Effector region

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rheb family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00730000110570.
    HOGENOMiHOG000233973.
    HOVERGENiHBG009351.
    InParanoidiQ921J2.
    KOiK07208.
    OMAiTILNSKH.
    OrthoDBiEOG7Q2N6K.
    PhylomeDBiQ921J2.
    TreeFamiTF314986.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR020849. Small_GTPase_Ras.
    [Graphical view]
    PANTHERiPTHR24070. PTHR24070. 1 hit.
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00173. RAS. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51421. RAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q921J2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPQSKSRKIA ILGYRSVGKS SLTIQFVEGQ FVDSYDPTIE NTFTKLITVN    50
    GQEYHLQLVD TAGQDEYSIF PQTYSIDING YILVYSVTSI KSFEVIKVIH 100
    GKLLDMVGKV QIPIMLVGNK KDLHMERVIS YEEGKALAES WNAAFLESSA 150
    KENQTAVDVF KRIILEAEKI DGAASQGKSS CSVM 184
    Length:184
    Mass (Da):20,451
    Last modified:December 1, 2001 - v1
    Checksum:i7EC58080BDF92DFB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC012273 mRNA. Translation: AAH12273.1.
    CCDSiCCDS39032.1.
    RefSeqiNP_444305.2. NM_053075.3.
    UniGeneiMm.319175.

    Genome annotation databases

    EnsembliENSMUST00000030787; ENSMUSP00000030787; ENSMUSG00000028945.
    GeneIDi19744.
    KEGGimmu:19744.
    UCSCiuc008wsj.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC012273 mRNA. Translation: AAH12273.1 .
    CCDSi CCDS39032.1.
    RefSeqi NP_444305.2. NM_053075.3.
    UniGenei Mm.319175.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4O25 X-ray 2.20 A/B 1-169 [» ]
    4O2L X-ray 2.40 A/B 1-169 [» ]
    4O2R X-ray 2.25 A/B 1-169 [» ]
    ProteinModelPortali Q921J2.
    SMRi Q921J2. Positions 3-169.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202889. 5 interactions.
    IntActi Q921J2. 2 interactions.
    MINTi MINT-4611176.

    PTM databases

    PhosphoSitei Q921J2.

    Proteomic databases

    MaxQBi Q921J2.
    PaxDbi Q921J2.
    PRIDEi Q921J2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030787 ; ENSMUSP00000030787 ; ENSMUSG00000028945 .
    GeneIDi 19744.
    KEGGi mmu:19744.
    UCSCi uc008wsj.1. mouse.

    Organism-specific databases

    CTDi 6009.
    MGIi MGI:97912. Rheb.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00730000110570.
    HOGENOMi HOG000233973.
    HOVERGENi HBG009351.
    InParanoidi Q921J2.
    KOi K07208.
    OMAi TILNSKH.
    OrthoDBi EOG7Q2N6K.
    PhylomeDBi Q921J2.
    TreeFami TF314986.

    Miscellaneous databases

    ChiTaRSi RHEB. mouse.
    NextBioi 297194.
    PROi Q921J2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q921J2.
    Bgeei Q921J2.
    CleanExi MM_RHEB.
    Genevestigatori Q921J2.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR020849. Small_GTPase_Ras.
    [Graphical view ]
    PANTHERi PTHR24070. PTHR24070. 1 hit.
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00173. RAS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51421. RAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    2. "Inactivation of Rheb by PRAK-mediated phosphorylation is essential for energy-depletion-induced suppression of mTORC1."
      Zheng M., Wang Y.H., Wu X.N., Wu S.Q., Lu B.J., Dong M.Q., Zhang H., Sun P., Lin S.C., Guan K.L., Han J.
      Nat. Cell Biol. 13:263-272(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-130, MUTAGENESIS OF SER-130.

    Entry informationi

    Entry nameiRHEB_MOUSE
    AccessioniPrimary (citable) accession number: Q921J2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2003
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The conserved catalytic Gln-64 found in other Ras-like GTPases seems not to be involved in GTP hydrolysis in RHEB.By similarity

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3