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Q921J2

- RHEB_MOUSE

UniProt

Q921J2 - RHEB_MOUSE

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Protein
GTP-binding protein Rheb
Gene
Rheb
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity By similarity.

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Inactivated by TSC1-TSC2 via the GTPase activating protein (GAP) domain of TSC2 By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi20 – 201Magnesium By similarity
Metal bindingi38 – 381Magnesium By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 216GTP By similarity
Nucleotide bindingi32 – 387GTP By similarity
Nucleotide bindingi149 – 1502GTP By similarity

GO - Molecular functioni

  1. GDP binding Source: Ensembl
  2. GTP binding Source: UniProtKB
  3. metal ion binding Source: UniProtKB-KW
  4. protein kinase binding Source: UniProtKB

GO - Biological processi

  1. GTP catabolic process Source: InterPro
  2. positive regulation of TOR signaling Source: UniProtKB
  3. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein Rheb
Alternative name(s):
Ras homolog enriched in brain
Gene namesi
Name:Rheb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:97912. Rheb.

Subcellular locationi

GO - Cellular componenti

  1. dendrite Source: Ensembl
  2. neuronal cell body Source: Ensembl
  3. plasma membrane Source: UniProtKB-SubCell
  4. spliceosomal complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi130 – 1301S → A: Abolishes phosphorylation by MAPKAPK5 and impairs GTP-binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 181181GTP-binding protein Rheb
PRO_0000082709Add
BLAST
Propeptidei182 – 1843Removed in mature form By similarity
PRO_0000281366

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei130 – 1301Phosphoserine; by MAPKAPK51 Publication
Modified residuei181 – 1811Cysteine methyl ester By similarity
Lipidationi181 – 1811S-farnesyl cysteine By similarity

Post-translational modificationi

Farnesylation is important for efficiently activating mTORC1-mediated signaling By similarity.
Phosphorylation by MAPKAPK5 impairs GTP-binding and inactivation.

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiQ921J2.
PaxDbiQ921J2.
PRIDEiQ921J2.

PTM databases

PhosphoSiteiQ921J2.

Expressioni

Gene expression databases

ArrayExpressiQ921J2.
BgeeiQ921J2.
CleanExiMM_RHEB.
GenevestigatoriQ921J2.

Interactioni

Subunit structurei

Binds to mTORC1 in a guanyl nucleotide-independent manner. Interacts directly with MTOR, MLST8 and RPTOR. Interacts with TSC2 By similarity.

Protein-protein interaction databases

BioGridi202889. 5 interactions.
IntActiQ921J2. 2 interactions.
MINTiMINT-4611176.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 1410
Helixi19 – 2810
Beta strandi41 – 499
Beta strandi52 – 609
Beta strandi80 – 867
Helixi90 – 10718
Beta strandi114 – 1196
Helixi124 – 1263
Helixi131 – 14010
Beta strandi145 – 1473
Helixi153 – 16715

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4O25X-ray2.20A/B1-169[»]
4O2LX-ray2.40A/B1-169[»]
4O2RX-ray2.25A/B1-169[»]
ProteinModelPortaliQ921J2.
SMRiQ921J2. Positions 3-169.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi35 – 439Effector region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00730000110570.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiQ921J2.
KOiK07208.
OMAiTILNSKH.
OrthoDBiEOG7Q2N6K.
PhylomeDBiQ921J2.
TreeFamiTF314986.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00173. RAS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q921J2-1 [UniParc]FASTAAdd to Basket

« Hide

MPQSKSRKIA ILGYRSVGKS SLTIQFVEGQ FVDSYDPTIE NTFTKLITVN    50
GQEYHLQLVD TAGQDEYSIF PQTYSIDING YILVYSVTSI KSFEVIKVIH 100
GKLLDMVGKV QIPIMLVGNK KDLHMERVIS YEEGKALAES WNAAFLESSA 150
KENQTAVDVF KRIILEAEKI DGAASQGKSS CSVM 184
Length:184
Mass (Da):20,451
Last modified:December 1, 2001 - v1
Checksum:i7EC58080BDF92DFB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC012273 mRNA. Translation: AAH12273.1.
CCDSiCCDS39032.1.
RefSeqiNP_444305.2. NM_053075.3.
UniGeneiMm.319175.

Genome annotation databases

EnsembliENSMUST00000030787; ENSMUSP00000030787; ENSMUSG00000028945.
GeneIDi19744.
KEGGimmu:19744.
UCSCiuc008wsj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC012273 mRNA. Translation: AAH12273.1 .
CCDSi CCDS39032.1.
RefSeqi NP_444305.2. NM_053075.3.
UniGenei Mm.319175.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4O25 X-ray 2.20 A/B 1-169 [» ]
4O2L X-ray 2.40 A/B 1-169 [» ]
4O2R X-ray 2.25 A/B 1-169 [» ]
ProteinModelPortali Q921J2.
SMRi Q921J2. Positions 3-169.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202889. 5 interactions.
IntActi Q921J2. 2 interactions.
MINTi MINT-4611176.

PTM databases

PhosphoSitei Q921J2.

Proteomic databases

MaxQBi Q921J2.
PaxDbi Q921J2.
PRIDEi Q921J2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000030787 ; ENSMUSP00000030787 ; ENSMUSG00000028945 .
GeneIDi 19744.
KEGGi mmu:19744.
UCSCi uc008wsj.1. mouse.

Organism-specific databases

CTDi 6009.
MGIi MGI:97912. Rheb.

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00730000110570.
HOGENOMi HOG000233973.
HOVERGENi HBG009351.
InParanoidi Q921J2.
KOi K07208.
OMAi TILNSKH.
OrthoDBi EOG7Q2N6K.
PhylomeDBi Q921J2.
TreeFami TF314986.

Miscellaneous databases

ChiTaRSi RHEB. mouse.
NextBioi 297194.
PROi Q921J2.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q921J2.
Bgeei Q921J2.
CleanExi MM_RHEB.
Genevestigatori Q921J2.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view ]
PANTHERi PTHR24070. PTHR24070. 1 hit.
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00173. RAS. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51421. RAS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. "Inactivation of Rheb by PRAK-mediated phosphorylation is essential for energy-depletion-induced suppression of mTORC1."
    Zheng M., Wang Y.H., Wu X.N., Wu S.Q., Lu B.J., Dong M.Q., Zhang H., Sun P., Lin S.C., Guan K.L., Han J.
    Nat. Cell Biol. 13:263-272(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-130, MUTAGENESIS OF SER-130.

Entry informationi

Entry nameiRHEB_MOUSE
AccessioniPrimary (citable) accession number: Q921J2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The conserved catalytic Gln-64 found in other Ras-like GTPases seems not to be involved in GTP hydrolysis in RHEB By similarity.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi