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Protein

GTP-binding protein Rheb

Gene

Rheb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activates the protein kinase activity of mTORC1, and thereby plays a role in the regulation of apoptosis. Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Has low intrinsic GTPase activity.Curated1 Publication

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Inactivated by TSC1-TSC2 via the GTPase activating protein (GAP) domain of TSC2.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi20Magnesium1 Publication1
Metal bindingi38MagnesiumBy similarity1
Binding sitei63GTP; via amide nitrogen1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi16 – 21GTP1 Publication6
Nucleotide bindingi32 – 38GTP1 Publication7
Nucleotide bindingi119 – 122GTP1 Publication4
Nucleotide bindingi149 – 150GTP1 Publication2

GO - Molecular functioni

  • GTP binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • protein kinase binding Source: UniProtKB

GO - Biological processi

  • positive regulation of oligodendrocyte differentiation Source: MGI
  • positive regulation of TOR signaling Source: UniProtKB
  • regulation of TOR signaling Source: CACAO
  • regulation of type B pancreatic cell development Source: MGI
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1632852. Macroautophagy.
R-MMU-165159. mTOR signalling.
R-MMU-166208. mTORC1-mediated signalling.
R-MMU-380972. Energy dependent regulation of mTOR by LKB1-AMPK.
R-MMU-5628897. TP53 Regulates Metabolic Genes.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein Rheb
Alternative name(s):
Ras homolog enriched in brain
Gene namesi
Name:Rheb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:97912. Rheb.

Subcellular locationi

  • Endomembrane system By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
  • Golgi apparatus membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
  • Cytoplasmcytosol By similarity
  • Endoplasmic reticulum membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi63G → A: Impairs GTPase activity and increases stimulation of the protein kinase activity of mTORC1. 1 Publication1
Mutagenesisi130S → A: Abolishes phosphorylation by MAPKAPK5 and impairs GTP-binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000827091 – 181GTP-binding protein RhebAdd BLAST181
PropeptideiPRO_0000281366182 – 184Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei130Phosphoserine; by MAPKAPK51 Publication1
Modified residuei181Cysteine methyl esterBy similarity1
Lipidationi181S-farnesyl cysteineBy similarity1

Post-translational modificationi

Farnesylation is important for efficiently activating mTORC1-mediated signaling.By similarity
Phosphorylation by MAPKAPK5 impairs GTP-binding and inactivation.1 Publication

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

EPDiQ921J2.
MaxQBiQ921J2.
PaxDbiQ921J2.
PRIDEiQ921J2.

PTM databases

iPTMnetiQ921J2.
PhosphoSitePlusiQ921J2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028945.
CleanExiMM_RHEB.
ExpressionAtlasiQ921J2. baseline and differential.
GenevisibleiQ921J2. MM.

Interactioni

Subunit structurei

Binds to mTORC1 in a guanyl nucleotide-independent manner. Interacts directly with MTOR, MLST8 and RPTOR. Interacts with TSC2 (By similarity). Interacts (when prenylated) with PDE6D; this promotes release from membranes (By similarity).By similarity

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi202889. 6 interactors.
IntActiQ921J2. 2 interactors.
MINTiMINT-4611176.
STRINGi10090.ENSMUSP00000030787.

Structurei

Secondary structure

1184
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 14Combined sources10
Helixi19 – 28Combined sources10
Beta strandi41 – 49Combined sources9
Beta strandi52 – 60Combined sources9
Beta strandi80 – 86Combined sources7
Helixi90 – 107Combined sources18
Beta strandi114 – 119Combined sources6
Helixi124 – 126Combined sources3
Helixi131 – 140Combined sources10
Beta strandi145 – 147Combined sources3
Helixi153 – 167Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4O25X-ray2.20A/B1-169[»]
4O2LX-ray2.40A/B1-169[»]
4O2RX-ray2.25A/B1-169[»]
ProteinModelPortaliQ921J2.
SMRiQ921J2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi35 – 43Effector region9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rheb family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133696.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiQ921J2.
KOiK07208.
OMAiNHLGTES.
OrthoDBiEOG091G0ONO.
PhylomeDBiQ921J2.
TreeFamiTF314986.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q921J2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQSKSRKIA ILGYRSVGKS SLTIQFVEGQ FVDSYDPTIE NTFTKLITVN
60 70 80 90 100
GQEYHLQLVD TAGQDEYSIF PQTYSIDING YILVYSVTSI KSFEVIKVIH
110 120 130 140 150
GKLLDMVGKV QIPIMLVGNK KDLHMERVIS YEEGKALAES WNAAFLESSA
160 170 180
KENQTAVDVF KRIILEAEKI DGAASQGKSS CSVM
Length:184
Mass (Da):20,451
Last modified:December 1, 2001 - v1
Checksum:i7EC58080BDF92DFB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC012273 mRNA. Translation: AAH12273.1.
CCDSiCCDS39032.1.
RefSeqiNP_444305.2. NM_053075.3.
UniGeneiMm.319175.

Genome annotation databases

EnsembliENSMUST00000030787; ENSMUSP00000030787; ENSMUSG00000028945.
GeneIDi19744.
KEGGimmu:19744.
UCSCiuc008wsj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC012273 mRNA. Translation: AAH12273.1.
CCDSiCCDS39032.1.
RefSeqiNP_444305.2. NM_053075.3.
UniGeneiMm.319175.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4O25X-ray2.20A/B1-169[»]
4O2LX-ray2.40A/B1-169[»]
4O2RX-ray2.25A/B1-169[»]
ProteinModelPortaliQ921J2.
SMRiQ921J2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202889. 6 interactors.
IntActiQ921J2. 2 interactors.
MINTiMINT-4611176.
STRINGi10090.ENSMUSP00000030787.

PTM databases

iPTMnetiQ921J2.
PhosphoSitePlusiQ921J2.

Proteomic databases

EPDiQ921J2.
MaxQBiQ921J2.
PaxDbiQ921J2.
PRIDEiQ921J2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030787; ENSMUSP00000030787; ENSMUSG00000028945.
GeneIDi19744.
KEGGimmu:19744.
UCSCiuc008wsj.1. mouse.

Organism-specific databases

CTDi6009.
MGIiMGI:97912. Rheb.

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133696.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiQ921J2.
KOiK07208.
OMAiNHLGTES.
OrthoDBiEOG091G0ONO.
PhylomeDBiQ921J2.
TreeFamiTF314986.

Enzyme and pathway databases

ReactomeiR-MMU-1632852. Macroautophagy.
R-MMU-165159. mTOR signalling.
R-MMU-166208. mTORC1-mediated signalling.
R-MMU-380972. Energy dependent regulation of mTOR by LKB1-AMPK.
R-MMU-5628897. TP53 Regulates Metabolic Genes.

Miscellaneous databases

ChiTaRSiRheb. mouse.
PROiQ921J2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028945.
CleanExiMM_RHEB.
ExpressionAtlasiQ921J2. baseline and differential.
GenevisibleiQ921J2. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRHEB_MOUSE
AccessioniPrimary (citable) accession number: Q921J2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The conserved catalytic Gln-64 found in other Ras-like GTPases seems not to be involved in GTP hydrolysis in RHEB.By similarity

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.