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Q921J2 (RHEB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTP-binding protein Rheb
Alternative name(s):
Ras homolog enriched in brain
Gene names
Name:Rheb
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length184 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity By similarity.

Enzyme regulation

Alternates between an inactive form bound to GDP and an active form bound to GTP. Inactivated by TSC1-TSC2 via the GTPase activating protein (GAP) domain of TSC2 By similarity.

Subunit structure

Binds to mTORC1 in a guanyl nucleotide-independent manner. Interacts directly with MTOR, MLST8 and RPTOR. Interacts with TSC2 By similarity.

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side Potential.

Post-translational modification

Farnesylation is important for efficiently activating mTORC1-mediated signaling By similarity.

Phosphorylation by MAPKAPK5 impairs GTP-binding and inactivation.

Miscellaneous

The conserved catalytic Gln-64 found in other Ras-like GTPases seems not to be involved in GTP hydrolysis in RHEB By similarity.

Sequence similarities

Belongs to the small GTPase superfamily. Rheb family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 181181GTP-binding protein Rheb
PRO_0000082709
Propeptide182 – 1843Removed in mature form By similarity
PRO_0000281366

Regions

Nucleotide binding16 – 216GTP By similarity
Nucleotide binding32 – 387GTP By similarity
Nucleotide binding149 – 1502GTP By similarity
Motif35 – 439Effector region

Sites

Metal binding201Magnesium By similarity
Metal binding381Magnesium By similarity

Amino acid modifications

Modified residue1301Phosphoserine; by MAPKAPK5 Ref.2
Modified residue1811Cysteine methyl ester By similarity
Lipidation1811S-farnesyl cysteine By similarity

Experimental info

Mutagenesis1301S → A: Abolishes phosphorylation by MAPKAPK5 and impairs GTP-binding. Ref.2

Secondary structure

....................... 184
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q921J2 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 7EC58080BDF92DFB

FASTA18420,451
        10         20         30         40         50         60 
MPQSKSRKIA ILGYRSVGKS SLTIQFVEGQ FVDSYDPTIE NTFTKLITVN GQEYHLQLVD 

        70         80         90        100        110        120 
TAGQDEYSIF PQTYSIDING YILVYSVTSI KSFEVIKVIH GKLLDMVGKV QIPIMLVGNK 

       130        140        150        160        170        180 
KDLHMERVIS YEEGKALAES WNAAFLESSA KENQTAVDVF KRIILEAEKI DGAASQGKSS 


CSVM 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]"Inactivation of Rheb by PRAK-mediated phosphorylation is essential for energy-depletion-induced suppression of mTORC1."
Zheng M., Wang Y.H., Wu X.N., Wu S.Q., Lu B.J., Dong M.Q., Zhang H., Sun P., Lin S.C., Guan K.L., Han J.
Nat. Cell Biol. 13:263-272(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-130, MUTAGENESIS OF SER-130.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC012273 mRNA. Translation: AAH12273.1.
CCDSCCDS39032.1.
RefSeqNP_444305.2. NM_053075.3.
UniGeneMm.319175.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4O25X-ray2.20A/B1-169[»]
4O2LX-ray2.40A/B1-169[»]
4O2RX-ray2.25A/B1-169[»]
ProteinModelPortalQ921J2.
SMRQ921J2. Positions 3-169.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202889. 5 interactions.
IntActQ921J2. 2 interactions.
MINTMINT-4611176.

PTM databases

PhosphoSiteQ921J2.

Proteomic databases

MaxQBQ921J2.
PaxDbQ921J2.
PRIDEQ921J2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030787; ENSMUSP00000030787; ENSMUSG00000028945.
GeneID19744.
KEGGmmu:19744.
UCSCuc008wsj.1. mouse.

Organism-specific databases

CTD6009.
MGIMGI:97912. Rheb.

Phylogenomic databases

eggNOGCOG1100.
GeneTreeENSGT00730000110570.
HOGENOMHOG000233973.
HOVERGENHBG009351.
InParanoidQ921J2.
KOK07208.
OMATILNSKH.
OrthoDBEOG7Q2N6K.
PhylomeDBQ921J2.
TreeFamTF314986.

Gene expression databases

ArrayExpressQ921J2.
BgeeQ921J2.
CleanExMM_RHEB.
GenevestigatorQ921J2.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERPTHR24070. PTHR24070. 1 hit.
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00173. RAS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRHEB. mouse.
NextBio297194.
PROQ921J2.
SOURCESearch...

Entry information

Entry nameRHEB_MOUSE
AccessionPrimary (citable) accession number: Q921J2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot