GTP-binding protein Rheb precursor - Mus musculus (Mouse)

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Q921J2 (RHEB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 98. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
GTP-binding protein Rheb

Alternative name(s):
Ras homolog enriched in brain

Gene names

Name:

Rheb

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity By similarity.
Enzyme regulation	Alternates between an inactive form bound to GDP and an active form bound to GTP. Inactivated by TSC1-TSC2 via the GTPase activating protein (GAP) domain of TSC2 By similarity.
Subunit structure	Binds to mTORC1 in a guanyl nucleotide-independent manner. Interacts directly with MTOR, MLST8 and RPTOR. Interacts with TSC2 By similarity.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Post-translational modification	Farnesylation is important for efficiently activating mTORC1-mediated signaling By similarity. Phosphorylation by MAPKAPK5 impairs GTP-binding and inactivation.
Miscellaneous	The conserved catalytic Gln-64 found in other Ras-like GTPases seems not to be involved in GTP hydrolysis in RHEB By similarity.
Sequence similarities	Belongs to the small GTPase superfamily. Rheb family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
PTM	Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of TOR signaling cascade Inferred from direct assay Ref.2. Source: UniProtKB small GTPase mediated signal transduction Inferred from electronic annotation. Source: Compara
Cellular_component	dendrite Inferred from electronic annotation. Source: Compara neuronal cell body Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell spliceosomal complex Inferred from direct assay PubMed 11555636. Source: MGI
Molecular_function	GDP binding Inferred from electronic annotation. Source: Compara GTP binding Inferred from direct assay Ref.2. Source: UniProtKB GTPase activity Inferred from electronic annotation. Source: Compara metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 181

181

GTP-binding protein Rheb

PRO_0000082709

Propeptide

182 – 184

Removed in mature form By similarity

PRO_0000281366

Regions

Nucleotide binding

16 – 21

GTP By similarity

Nucleotide binding

32 – 38

GTP By similarity

Nucleotide binding

149 – 150

GTP By similarity

Motif

35 – 43

Effector region By similarity

Sites

Metal binding

Magnesium By similarity

Metal binding

Magnesium By similarity

Amino acid modifications

Modified residue

130

Phosphoserine; by MAPKAPK5 Ref.2

Modified residue

181

Cysteine methyl ester By similarity

Lipidation

181

S-farnesyl cysteine By similarity

Experimental info

Mutagenesis

130

S → A: Abolishes phosphorylation by MAPKAPK5 and impairs GTP-binding. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q921J2 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 7EC58080BDF92DFB
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FASTA

184

20,451

        10         20         30         40         50         60 
MPQSKSRKIA ILGYRSVGKS SLTIQFVEGQ FVDSYDPTIE NTFTKLITVN GQEYHLQLVD 

        70         80         90        100        110        120 
TAGQDEYSIF PQTYSIDING YILVYSVTSI KSFEVIKVIH GKLLDMVGKV QIPIMLVGNK 

       130        140        150        160        170        180 
KDLHMERVIS YEEGKALAES WNAAFLESSA KENQTAVDVF KRIILEAEKI DGAASQGKSS 


CSVM

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"Inactivation of Rheb by PRAK-mediated phosphorylation is essential for energy-depletion-induced suppression of mTORC1." Zheng M., Wang Y.H., Wu X.N., Wu S.Q., Lu B.J., Dong M.Q., Zhang H., Sun P., Lin S.C., Guan K.L., Han J. Nat. Cell Biol. 13:263-272(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-130, MUTAGENESIS OF SER-130.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC012273 mRNA. Translation: AAH12273.1.
IPI	IPI00177205.
RefSeq	NP_444305.2. NM_053075.3.
UniGene	Mm.319175.
3D structure databases
ProteinModelPortal	Q921J2.
SMR	Q921J2. Positions 6-174.
ModBase	Search...
PTM databases
PhosphoSite	Q921J2.
Proteomic databases
PaxDb	Q921J2.
PRIDE	Q921J2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000030787; ENSMUSP00000030787; ENSMUSG00000028945.
GeneID	19744.
KEGG	mmu:19744.
Organism-specific databases
CTD	6009.
MGI	MGI:97912. Rheb.
Phylogenomic databases
eggNOG	COG1100.
GeneTree	ENSGT00660000095554.
HOGENOM	HOG000233973.
HOVERGEN	HBG009351.
InParanoid	Q921J2.
KO	K07208.
OMA	ERNIAMM.
OrthoDB	EOG4VMFGG.
Gene expression databases
ArrayExpress	Q921J2.
Bgee	Q921J2.
CleanEx	MM_RHEB.
Genevestigator	Q921J2.
GermOnline	ENSMUSG00000028945. Mus musculus.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR020849. Small_GTPase_Ras. [Graphical view]
PANTHER	PTHR24070. PTHR24070. 1 hit.
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00173. RAS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51421. RAS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RHEB. mouse.
NextBio	297194.
SOURCE	Search...

Entry information

Entry name

RHEB_MOUSE

Accession

Primary (citable) accession number: Q921J2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2003
Last sequence update:	December 1, 2001
Last modified:	April 3, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents