Reviewed, UniProtKB/Swiss-Prot Q921H8 (THIKA_MOUSE)

Names and origin

Protein names

Recommended name:
    3-ketoacyl-CoA thiolase A, peroxisomal
    EC=2.3.1.16
Alternative name(s):
    Beta-ketothiolase A
    Acetyl-CoA acyltransferase A
    Peroxisomal 3-oxoacyl-CoA thiolase A

Gene names

Name:	Acaa1a
Synonyms:	Acaa1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.
Pathway	Lipid metabolism; fatty acid metabolism.
Subunit structure	Homodimer By similarity.
Subcellular location	Peroxisome By similarity.
Tissue specificity	Mainly expressed in liver and intestine. Ref.1
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Peroxisome
Domain	Transit peptide
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	fatty acid metabolic process Traceable author statement. Source: MGI
Cellular component	mitochondrion Inferred from direct assay. Source: MGI peroxisome Traceable author statement. Source: MGI
Molecular function	acetyl-CoA C-acetyltransferase activity Traceable author statement. Source: MGI acetyl-CoA C-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 26	26	Peroxisome By similarity
	Chain	27 – 424	398	3-ketoacyl-CoA thiolase A, peroxisomal		PRO_0000034068
Sites
	Active site	123	1	Acyl-thioester intermediate By similarity
	Active site	377	1	Proton acceptor By similarity
	Active site	408	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q921H8-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 56F30DF43198C9A8	FASTA	424	43,953
	10 20 30 40 50 60 MHRLQVVLGH LAGRPESSSA LQAAPCSARF PQASASDVVV VHGRRTPIGR ASRGGFKNTT 70 80 90 100 110 120 PDELLSAVLT AVLQDVRLKP EQLGDISVGN VLEPGAGAVM ARIAQFLSGI PETVPLSTVN 130 140 150 160 170 180 RQCSSGLQAV ANIAGGIRNG SYDIGMACGV ESMSLSGMGN PGNISSRLLE SEKARDCLTP 190 200 210 220 230 240 MGMTSENVAE RFGISRQKQD DFALASQQKA ASAQSRGCFR AEIVPVTTTV LDDKGDKKTI 250 260 270 280 290 300 TVSQDEGVRP STTMQGLAKL KPAFKDGGST TAGNSSQVSD GAAAVLLARR SKAEELGLPI 310 320 330 340 350 360 LGVLRSYAVV GVPPDVMGIG PAYAIPAALQ KAGLTVNDID IFEINEAFAS QAVYCVEKLG 370 380 390 400 410 420 IPAEKVNPLG GAIALGHPLG CTGARQVVTL LNELKRRGRR AYGVVSMCIG TGMGAAAVFE YPGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, gene structure and expression profile of two mouse peroxisomal 3-ketoacyl-CoA thiolase genes." Chevillard G., Clemencet M.-C., Etienne P., Martin P., Pineau T., Latruffe N., Nicolas-Frances V. BMC Biochem. 5:3-3(2004) [PubMed: 15043762] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY. Strain: 129/Sv and C57BL/6J. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY273811 mRNA. Translation: AAP31668.1. AY304542 Genomic DNA. Translation: AAP72964.1. BC012400 mRNA. Translation: AAH12400.1.
IPI	IPI00121833.
RefSeq	NP_570934.1.
UniGene	Mm.205266
3D structure databases
SMR	Q921H8. Positions 31-424.
ModBase	Search...
Protein-protein interaction databases
STRING	Q921H8.
Proteomic databases
PRIDE	Q921H8.
Genome annotation databases
Ensembl	ENSMUST00000039784; ENSMUSP00000042351; ENSMUSG00000036138; Mus musculus. [Genome view]
GeneID	113868.
KEGG	mmu:113868.
NMPDR	fig|10090.3.peg.21029.
UCSC	uc009sas.1. mouse.
Organism-specific databases
CTD	113868.
MGI	MGI:2148491. Acaa1a.
Phylogenomic databases
HOGENOM	HBG370930.
HOVERGEN	Q921H8.
InParanoid	Q921H8.
OMA	ANCKVPM.
OrthoDB	EOG970X2X.
PhylomeDB	Q921H8.
Enzyme and pathway databases
BRENDA	2.3.1.16. 244.
Gene expression databases
ArrayExpress	Q921H8.
Bgee	Q921H8.
CleanEx	MM_ACAA1A.
Genevestigator	Q921H8.
GermOnline	ENSMUSG00000036138. Mus musculus.
Family and domain databases
InterPro	IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020615. Thiolase_acyl_enz_int_AS. IPR020610. Thiolase_AS. IPR020617. Thiolase_C. IPR020613. Thiolase_CS. IPR020616. Thiolase_N. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 1 hit.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	368045.
SOURCE	Search...

Entry information

Entry name

THIKA_MOUSE

Accession

Primary (citable) accession number: Q921H8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	December 1, 2001
Last modified:	February 9, 2010
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)