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Protein

Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial

Gene

Etfdh

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accepts electrons from ETF and reduces ubiquinone.By similarity

Catalytic activityi

Reduced electron-transferring flavoprotein + ubiquinone = electron-transferring flavoprotein + ubiquinol.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei304Ubiquinone; via carbonyl oxygenBy similarity1
Binding sitei305Ubiquinone; via amide nitrogenBy similarity1
Metal bindingi560Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi585Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi588Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi591Iron-sulfur (4Fe-4S)Sequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi70 – 84FADSequence analysisAdd BLAST15

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

4Fe-4S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, Ubiquinone

Enzyme and pathway databases

ReactomeiR-MMU-611105. Respiratory electron transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial (EC:1.5.5.1)
Short name:
ETF-QO
Short name:
ETF-ubiquinone oxidoreductase
Alternative name(s):
Electron-transferring-flavoprotein dehydrogenase
Short name:
ETF dehydrogenase
Gene namesi
Name:Etfdh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:106100. Etfdh.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Intramembranei108 – 129By similarityAdd BLAST22
Intramembranei427 – 446By similarityAdd BLAST20

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 32MitochondrionSequence analysisAdd BLAST32
ChainiPRO_000000866233 – 616Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrialAdd BLAST584

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei95N6-acetyllysineCombined sources1
Modified residuei131N6-acetyllysineCombined sources1
Modified residuei222N6-acetyllysineCombined sources1
Modified residuei356N6-acetyllysineCombined sources1
Modified residuei415N6-acetyllysineCombined sources1
Modified residuei550PhosphoserineCombined sources1

Post-translational modificationi

Acetylation of Lys-95 and Lys-222 is observed in liver mitochondria from fasted mice but not from fed mice.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ921G7.
MaxQBiQ921G7.
PaxDbiQ921G7.
PeptideAtlasiQ921G7.
PRIDEiQ921G7.

PTM databases

iPTMnetiQ921G7.
PhosphoSitePlusiQ921G7.
SwissPalmiQ921G7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027809.
CleanExiMM_ETFDH.
ExpressionAtlasiQ921G7. baseline and differential.
GenevisibleiQ921G7. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiQ921G7. 3 interactors.
MINTiMINT-1844957.
STRINGi10090.ENSMUSP00000029386.

Structurei

3D structure databases

ProteinModelPortaliQ921G7.
SMRiQ921G7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini576 – 6054Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd BLAST30

Sequence similaritiesi

Belongs to the ETF-QO/FixC family.Curated
Contains 1 4Fe-4S ferredoxin-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2415. Eukaryota.
COG0644. LUCA.
GeneTreeiENSGT00390000010773.
HOGENOMiHOG000259450.
HOVERGENiHBG005615.
InParanoidiQ921G7.
KOiK00311.
OMAiPKTTFER.
OrthoDBiEOG091G03C7.
PhylomeDBiQ921G7.
TreeFamiTF105687.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR007859. ETFD_OxRdtase/FixX.
IPR023753. FAD/NAD-binding_dom.
IPR000103. Pyridine_nuc-diS_OxRdtase_2.
[Graphical view]
PANTHERiPTHR10617:SF107. PTHR10617:SF107. 1 hit.
PfamiPF05187. ETF_QO. 1 hit.
[Graphical view]
PRINTSiPR00469. PNDRDTASEII.
SUPFAMiSSF51905. SSF51905. 2 hits.
PROSITEiPS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q921G7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVRLTKLSC PAYHWFHALK IKKCLPLCAP RCSSTSAVPQ ITTHYTVHPR
60 70 80 90 100
EKDKRWEGVN MERFAEEADV VIVGAGPAGL SAAIRLKQLA AEQGKDIRVC
110 120 130 140 150
LVEKAAQIGA HTLSGACLDP AAFKELFPDW KEKGAPLNTP VTEDRFAILT
160 170 180 190 200
EKHRIPVPIL PGLPMNNHGN YIVRLGHLVS WMGEQAEALG VEVYPGYAAA
210 220 230 240 250
EVLYHEDGSV KGIATNDVGI QKDGAPKTTF ERGLELHAKV TVFAEGCHGH
260 270 280 290 300
LAKQLYKKFD LRASCDAQTY GIGLKELWII DEKKWKPGRV DHTVGWPLDR
310 320 330 340 350
HTYGGSFLYH LNEGEPLVAV GFVVGLDYQN PYLSPFREFQ RWKHHPSIQP
360 370 380 390 400
TLEGGKRIAY GARALNEGGL QSIPKLTFPG GLLIGCSPGF MNVPKIKGTH
410 420 430 440 450
TAMKSGSLAA ESIFKQLTSE NLQSKTTGLH VTEYEDNLKQ SWVWKELHAV
460 470 480 490 500
RNIRPSCHGI LGVYGGMIYT GIFYWILRGM EPWTLKHKGS DSDQLKPAKD
510 520 530 540 550
CTPIEYPKPD GQISFDLLSS VALSGTNHEH DQPAHLTLKD DSIPVNRNLS
560 570 580 590 600
IYDGPEQRFC PAGVYEFVPL EQGDGFRLQI NAQNCVHCKT CDIKDPSQNI
610
NWVVPEGGGG PAYNGM
Length:616
Mass (Da):68,091
Last modified:December 1, 2001 - v1
Checksum:i1E5C6F85725E3CEE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti342W → C in BAB22135 (PubMed:16141072).Curated1
Sequence conflicti355G → W in BAC35888 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002483 mRNA. Translation: BAB22135.1.
AK075673 mRNA. Translation: BAC35888.1.
AK141684 mRNA. Translation: BAE24798.1.
AK152624 mRNA. Translation: BAE31367.1.
AK169686 mRNA. Translation: BAE41304.1.
BC012522 mRNA. Translation: AAH12522.1.
CCDSiCCDS17418.1.
RefSeqiNP_080070.2. NM_025794.2.
UniGeneiMm.28336.

Genome annotation databases

EnsembliENSMUST00000029386; ENSMUSP00000029386; ENSMUSG00000027809.
GeneIDi66841.
KEGGimmu:66841.
UCSCiuc008pnq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002483 mRNA. Translation: BAB22135.1.
AK075673 mRNA. Translation: BAC35888.1.
AK141684 mRNA. Translation: BAE24798.1.
AK152624 mRNA. Translation: BAE31367.1.
AK169686 mRNA. Translation: BAE41304.1.
BC012522 mRNA. Translation: AAH12522.1.
CCDSiCCDS17418.1.
RefSeqiNP_080070.2. NM_025794.2.
UniGeneiMm.28336.

3D structure databases

ProteinModelPortaliQ921G7.
SMRiQ921G7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ921G7. 3 interactors.
MINTiMINT-1844957.
STRINGi10090.ENSMUSP00000029386.

PTM databases

iPTMnetiQ921G7.
PhosphoSitePlusiQ921G7.
SwissPalmiQ921G7.

Proteomic databases

EPDiQ921G7.
MaxQBiQ921G7.
PaxDbiQ921G7.
PeptideAtlasiQ921G7.
PRIDEiQ921G7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029386; ENSMUSP00000029386; ENSMUSG00000027809.
GeneIDi66841.
KEGGimmu:66841.
UCSCiuc008pnq.2. mouse.

Organism-specific databases

CTDi2110.
MGIiMGI:106100. Etfdh.

Phylogenomic databases

eggNOGiKOG2415. Eukaryota.
COG0644. LUCA.
GeneTreeiENSGT00390000010773.
HOGENOMiHOG000259450.
HOVERGENiHBG005615.
InParanoidiQ921G7.
KOiK00311.
OMAiPKTTFER.
OrthoDBiEOG091G03C7.
PhylomeDBiQ921G7.
TreeFamiTF105687.

Enzyme and pathway databases

ReactomeiR-MMU-611105. Respiratory electron transport.

Miscellaneous databases

PROiQ921G7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027809.
CleanExiMM_ETFDH.
ExpressionAtlasiQ921G7. baseline and differential.
GenevisibleiQ921G7. MM.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR007859. ETFD_OxRdtase/FixX.
IPR023753. FAD/NAD-binding_dom.
IPR000103. Pyridine_nuc-diS_OxRdtase_2.
[Graphical view]
PANTHERiPTHR10617:SF107. PTHR10617:SF107. 1 hit.
PfamiPF05187. ETF_QO. 1 hit.
[Graphical view]
PRINTSiPR00469. PNDRDTASEII.
SUPFAMiSSF51905. SSF51905. 2 hits.
PROSITEiPS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiETFD_MOUSE
AccessioniPrimary (citable) accession number: Q921G7
Secondary accession number(s): Q3U7K2, Q8BK82, Q9DCT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.