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Protein

Mediator of RNA polymerase II transcription subunit 6

Gene

Med6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-212436. Generic Transcription Pathway.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 6
Alternative name(s):
Mediator complex subunit 6
Gene namesi
Name:Med6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1917042. Med6.

Subcellular locationi

GO - Cellular componenti

  • mediator complex Source: MGI
  • membrane Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 246246Mediator of RNA polymerase II transcription subunit 6PRO_0000303042Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei236 – 2361N6-acetyllysineCombined sources
Modified residuei241 – 2411N6-acetyllysineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ921D4.
MaxQBiQ921D4.
PaxDbiQ921D4.
PRIDEiQ921D4.

PTM databases

iPTMnetiQ921D4.
PhosphoSiteiQ921D4.

Expressioni

Gene expression databases

BgeeiQ921D4.
ExpressionAtlasiQ921D4. baseline and differential.
GenevisibleiQ921D4. MM.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with CTNNB1 and GLI3 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi213683. 2 interactions.
DIPiDIP-60703N.
IntActiQ921D4. 4 interactions.
MINTiMINT-217697.
STRINGi10090.ENSMUSP00000002756.

Structurei

3D structure databases

ProteinModelPortaliQ921D4.
SMRiQ921D4. Positions 13-150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 6 family.Curated

Phylogenomic databases

eggNOGiKOG3169. Eukaryota.
COG5097. LUCA.
GeneTreeiENSGT00390000017666.
HOGENOMiHOG000035127.
HOVERGENiHBG006825.
InParanoidiQ921D4.
KOiK15128.
OMAiQTVMDYF.
OrthoDBiEOG7RNK17.
PhylomeDBiQ921D4.
TreeFamiTF313577.

Family and domain databases

InterProiIPR007018. Mediator_Med6.
IPR016820. Mediator_Med6_met/pln.
[Graphical view]
PANTHERiPTHR13104. PTHR13104. 1 hit.
PfamiPF04934. Med6. 1 hit.
[Graphical view]
PIRSFiPIRSF023869. Mediator_MED6_meta/pln. 1 hit.

Sequencei

Sequence statusi: Complete.

Q921D4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVDIRDNL LGISWVDSSW IPILNSGSVL DYFSERSNPF YDRTCNNEVV
60 70 80 90 100
KMQRLTLEHL NQMVGIEYIL LHAQEPILFI IRKQQRQSPA QVIPLADYYI
110 120 130 140 150
IAGVIYQAPD LGSVINSRVL TAVHGIQSAF DEAMSYCRYH PSKGYWWHFK
160 170 180 190 200
DHEEQEKVKP KAKRKEEPSS IFQRQRVDAL LIDLRQKFPP RFVQQKSGEK
210 220 230 240
PVPVDQAKKE AEPLPETVKS EEKESTKNIQ QTVSTKGPPE KRMRLQ
Length:246
Mass (Da):28,434
Last modified:September 11, 2007 - v2
Checksum:i51DB8A599B24DCF6
GO

Sequence cautioni

The sequence AAH13096.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAE42663.1 differs from that shown. Reason: Frameshift at position 210. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK089101 mRNA. Translation: BAC40749.1.
AK171784 mRNA. Translation: BAE42663.1. Frameshift.
BC013096 mRNA. Translation: AAH13096.1. Different initiation.
RefSeqiNP_081489.2. NM_027213.2.
XP_006516274.1. XM_006516211.2.
XP_011242473.1. XM_011244171.1.
UniGeneiMm.279993.

Genome annotation databases

EnsembliENSMUST00000161211; ENSMUSP00000125156; ENSMUSG00000002679.
GeneIDi69792.
KEGGimmu:69792.
UCSCiuc007ocl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK089101 mRNA. Translation: BAC40749.1.
AK171784 mRNA. Translation: BAE42663.1. Frameshift.
BC013096 mRNA. Translation: AAH13096.1. Different initiation.
RefSeqiNP_081489.2. NM_027213.2.
XP_006516274.1. XM_006516211.2.
XP_011242473.1. XM_011244171.1.
UniGeneiMm.279993.

3D structure databases

ProteinModelPortaliQ921D4.
SMRiQ921D4. Positions 13-150.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213683. 2 interactions.
DIPiDIP-60703N.
IntActiQ921D4. 4 interactions.
MINTiMINT-217697.
STRINGi10090.ENSMUSP00000002756.

PTM databases

iPTMnetiQ921D4.
PhosphoSiteiQ921D4.

Proteomic databases

EPDiQ921D4.
MaxQBiQ921D4.
PaxDbiQ921D4.
PRIDEiQ921D4.

Protocols and materials databases

DNASUi69792.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000161211; ENSMUSP00000125156; ENSMUSG00000002679.
GeneIDi69792.
KEGGimmu:69792.
UCSCiuc007ocl.1. mouse.

Organism-specific databases

CTDi10001.
MGIiMGI:1917042. Med6.

Phylogenomic databases

eggNOGiKOG3169. Eukaryota.
COG5097. LUCA.
GeneTreeiENSGT00390000017666.
HOGENOMiHOG000035127.
HOVERGENiHBG006825.
InParanoidiQ921D4.
KOiK15128.
OMAiQTVMDYF.
OrthoDBiEOG7RNK17.
PhylomeDBiQ921D4.
TreeFamiTF313577.

Enzyme and pathway databases

ReactomeiR-MMU-212436. Generic Transcription Pathway.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

ChiTaRSiMed6. mouse.
PROiQ921D4.
SOURCEiSearch...

Gene expression databases

BgeeiQ921D4.
ExpressionAtlasiQ921D4. baseline and differential.
GenevisibleiQ921D4. MM.

Family and domain databases

InterProiIPR007018. Mediator_Med6.
IPR016820. Mediator_Med6_met/pln.
[Graphical view]
PANTHERiPTHR13104. PTHR13104. 1 hit.
PfamiPF04934. Med6. 1 hit.
[Graphical view]
PIRSFiPIRSF023869. Mediator_MED6_meta/pln. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: NOD.
    Tissue: Spleen and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-246.
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-236 AND LYS-241, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiMED6_MOUSE
AccessioniPrimary (citable) accession number: Q921D4
Secondary accession number(s): Q3TAK5, Q8BTP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: June 8, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.