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Protein

Mediator of RNA polymerase II transcription subunit 6

Gene

Med6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-212436. Generic Transcription Pathway.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 6
Alternative name(s):
Mediator complex subunit 6
Gene namesi
Name:Med6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1917042. Med6.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003030421 – 246Mediator of RNA polymerase II transcription subunit 6Add BLAST246

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki208Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei236N6-acetyllysineCombined sources1
Modified residuei241N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ921D4.
PaxDbiQ921D4.
PeptideAtlasiQ921D4.
PRIDEiQ921D4.

PTM databases

iPTMnetiQ921D4.
PhosphoSitePlusiQ921D4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000002679.
ExpressionAtlasiQ921D4. baseline and differential.
GenevisibleiQ921D4. MM.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with CTNNB1 and GLI3 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi213683. 2 interactors.
CORUMiQ921D4.
DIPiDIP-60703N.
IntActiQ921D4. 5 interactors.
MINTiMINT-217697.
STRINGi10090.ENSMUSP00000002756.

Structurei

3D structure databases

ProteinModelPortaliQ921D4.
SMRiQ921D4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 6 family.Curated

Phylogenomic databases

eggNOGiKOG3169. Eukaryota.
COG5097. LUCA.
GeneTreeiENSGT00390000017666.
HOGENOMiHOG000035127.
HOVERGENiHBG006825.
InParanoidiQ921D4.
KOiK15128.
OMAiYARYHPN.
OrthoDBiEOG091G0MJA.
PhylomeDBiQ921D4.
TreeFamiTF313577.

Family and domain databases

InterProiView protein in InterPro
IPR007018. Mediator_Med6.
IPR016820. Mediator_Med6_met/pln.
PANTHERiPTHR13104. PTHR13104. 1 hit.
PfamiView protein in Pfam
PF04934. Med6. 1 hit.
PIRSFiPIRSF023869. Mediator_MED6_meta/pln. 1 hit.

Sequencei

Sequence statusi: Complete.

Q921D4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVDIRDNL LGISWVDSSW IPILNSGSVL DYFSERSNPF YDRTCNNEVV
60 70 80 90 100
KMQRLTLEHL NQMVGIEYIL LHAQEPILFI IRKQQRQSPA QVIPLADYYI
110 120 130 140 150
IAGVIYQAPD LGSVINSRVL TAVHGIQSAF DEAMSYCRYH PSKGYWWHFK
160 170 180 190 200
DHEEQEKVKP KAKRKEEPSS IFQRQRVDAL LIDLRQKFPP RFVQQKSGEK
210 220 230 240
PVPVDQAKKE AEPLPETVKS EEKESTKNIQ QTVSTKGPPE KRMRLQ
Length:246
Mass (Da):28,434
Last modified:September 11, 2007 - v2
Checksum:i51DB8A599B24DCF6
GO

Sequence cautioni

The sequence AAH13096 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE42663 differs from that shown. Reason: Frameshift at position 210.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK089101 mRNA. Translation: BAC40749.1.
AK171784 mRNA. Translation: BAE42663.1. Frameshift.
BC013096 mRNA. Translation: AAH13096.1. Different initiation.
CCDSiCCDS83983.1.
RefSeqiNP_001334313.1. NM_001347384.1.
NP_001334315.1. NM_001347386.1.
NP_081489.2. NM_027213.2.
UniGeneiMm.279993.

Genome annotation databases

EnsembliENSMUST00000161211; ENSMUSP00000125156; ENSMUSG00000002679.
GeneIDi69792.
KEGGimmu:69792.
UCSCiuc007ocl.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiMED6_MOUSE
AccessioniPrimary (citable) accession number: Q921D4
Secondary accession number(s): Q3TAK5, Q8BTP9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: November 22, 2017
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families