Q921C5 (BICD2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein bicaudal D homolog 2
Short name=Bic-D 2

Gene names

Name:	Bicd2
Synonyms:	Kiaa0699

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	820 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in the dynein-dynactin interactions on the surface of membranous organelles, by associating with these complexes. Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport by recruiting the dynein-dynactin motor complex.
Subunit structure	Interacts with NEK9 By similarity. Interacts with DCTN2 and RAB6A.
Subcellular location	Golgi apparatus. Cytoplasm › cytoskeleton. Note: In interphase cells mainly localizes to the Golgi complex and colocalizes with dynactin at microtubule plus ends.
Domain	The fourth coiled coil region is involved in Golgi targeting and in the interaction with DCTN2.
Post-translational modification	Phosphorylated by NEK9 in vitro By similarity.
Sequence similarities	Belongs to the BicD family.
Sequence caution	The sequence BAC65630.1 differs from that shown. Reason: Erroneous initiation. Isoform 3: The sequence BAB22282.1 differs from that shown. Reason: Frameshift at position 70.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Golgi apparatus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	microtubule anchoring at microtubule organizing center Inferred from mutant phenotype PubMed 19825938. Source: BHF-UCL minus-end-directed organelle transport along microtubule Inferred from mutant phenotype Ref.5. Source: BHF-UCL
Cellular_component	Golgi apparatus Inferred from direct assay Ref.5. Source: BHF-UCL cytoplasmic vesicle Inferred from direct assay Ref.5. Source: BHF-UCL cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q921C5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q921C5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     820-820: L → VSHTCACASERAEGAGLANQVFCSEKHSIYCD

Note: No experimental confirmation available.

Isoform 3 (identifier: Q921C5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     328-338: APPSPSLVSDL → VHPLHALCLTV
     339-820: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 820	820	Protein bicaudal D homolog 2		PRO_0000205360
Regions
	Region	662 – 804	143	Interacts with RAB6A
	Coiled coil	20 – 270	251	Potential
	Coiled coil	340 – 539	200	Potential
	Coiled coil	662 – 804	143	Potential
Amino acid modifications
	Modified residue	190	1	Phosphoserine By similarity
	Modified residue	224	1	Phosphoserine By similarity
	Modified residue	345	1	Phosphoserine By similarity
	Modified residue	397	1	Phosphoserine By similarity
	Modified residue	578	1	Phosphoserine By similarity
	Modified residue	819	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	328 – 338	11	APPSPSLVSDL → VHPLHALCLTV in isoform 3.		VSP_007971
	Alternative sequence	339 – 820	482	Missing in isoform 3.		VSP_007972
	Alternative sequence	820	1	L → VSHTCACASERAEGAGLANQ VFCSEKHSIYCD in isoform 2.		VSP_007970

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 0C1A1754CD74DDE1	FASTA	820	93,391
	10 20 30 40 50 60 MSAPSEEEEY ARLVMEAQPE WLRAEVKRLS HELAETTREK IQAAEYGLAV LEEKHQLKLQ 70 80 90 100 110 120 FEELEVDYEA IRSEMEQLKE AFGQAHTNHK KVAADGESRE ESLIQESASK EQYYVRKVLE 130 140 150 160 170 180 LQTELKQLRN VLTNTQSENE RLTSVAQELK EINQNVEIQR GRLRDDIKEY KFREARLLQD 190 200 210 220 230 240 YSELEEENIS LQKQVSVLRQ NQVEFEGLKH EIKRLEEETE YLNSQLEDAI RLKEISERQL 250 260 270 280 290 300 EEALETLKTE REQKNNLRKE LSHYMSINDS FYTSHLQVSL DGLKFSDDTV TAEPNNDAEA 310 320 330 340 350 360 LVNGFEHSGL VKSSLDNKTS TPRKDGLAPP SPSLVSDLLS ELHISEIQKL KQQLVQMERE 370 380 390 400 410 420 KVGLLATLQD TQKQLEQARG TLSEQHEKVN RLTENLSALR RLQAGKERQT SLDNEKDRDS 430 440 450 460 470 480 HEDGDYYEVD INGPEILACK YHVAVAEAGE LREQLKALRS THEAREAQHA EEKGRYEAEG 490 500 510 520 530 540 QALTEKISLL EKASHQDREL LAHLEKELKK VSDVAGETQG SLNVAQDELV TFSEELANLY 550 560 570 580 590 600 HHVCMCNNET PNRVMLDYYR EGQGKAGRTS PEGRGRRSPV LLPKGLLATE VGRADGGTGD 610 620 630 640 650 660 NSPSPSSSLP SPLSDPRREP MNIYNLIAII RDQIKHLQAA VDRTTELSRQ RIASQELGPA 670 680 690 700 710 720 VDKDKEALME EILKLKSLLS TKREQITTLR TVLKANKQTA EVALANLKSK YENEKAMVTE 730 740 750 760 770 780 TMMKLRNELK ALKEDAATFS SLRAMFATRC DEYITQLDEM QRQLAAAEDE KKTLNSLLRM 790 800 810 820 AIQQKLALTQ RLELLELDHE QTRRGRSKAA SKAKPASPSL
	Isoform 2 [UniParc]. Checksum: 25A80CD34D97587F	FASTA	851	96,647
	10 20 30 40 50 60 MSAPSEEEEY ARLVMEAQPE WLRAEVKRLS HELAETTREK IQAAEYGLAV LEEKHQLKLQ 70 80 90 100 110 120 FEELEVDYEA IRSEMEQLKE AFGQAHTNHK KVAADGESRE ESLIQESASK EQYYVRKVLE 130 140 150 160 170 180 LQTELKQLRN VLTNTQSENE RLTSVAQELK EINQNVEIQR GRLRDDIKEY KFREARLLQD 190 200 210 220 230 240 YSELEEENIS LQKQVSVLRQ NQVEFEGLKH EIKRLEEETE YLNSQLEDAI RLKEISERQL 250 260 270 280 290 300 EEALETLKTE REQKNNLRKE LSHYMSINDS FYTSHLQVSL DGLKFSDDTV TAEPNNDAEA 310 320 330 340 350 360 LVNGFEHSGL VKSSLDNKTS TPRKDGLAPP SPSLVSDLLS ELHISEIQKL KQQLVQMERE 370 380 390 400 410 420 KVGLLATLQD TQKQLEQARG TLSEQHEKVN RLTENLSALR RLQAGKERQT SLDNEKDRDS 430 440 450 460 470 480 HEDGDYYEVD INGPEILACK YHVAVAEAGE LREQLKALRS THEAREAQHA EEKGRYEAEG 490 500 510 520 530 540 QALTEKISLL EKASHQDREL LAHLEKELKK VSDVAGETQG SLNVAQDELV TFSEELANLY 550 560 570 580 590 600 HHVCMCNNET PNRVMLDYYR EGQGKAGRTS PEGRGRRSPV LLPKGLLATE VGRADGGTGD 610 620 630 640 650 660 NSPSPSSSLP SPLSDPRREP MNIYNLIAII RDQIKHLQAA VDRTTELSRQ RIASQELGPA 670 680 690 700 710 720 VDKDKEALME EILKLKSLLS TKREQITTLR TVLKANKQTA EVALANLKSK YENEKAMVTE 730 740 750 760 770 780 TMMKLRNELK ALKEDAATFS SLRAMFATRC DEYITQLDEM QRQLAAAEDE KKTLNSLLRM 790 800 810 820 830 840 AIQQKLALTQ RLELLELDHE QTRRGRSKAA SKAKPASPSV SHTCACASER AEGAGLANQV 850 FCSEKHSIYC D
	Isoform 3 [UniParc]. Checksum: E2DF08B87A283BFE	FASTA	338	39,315
	10 20 30 40 50 60 MSAPSEEEEY ARLVMEAQPE WLRAEVKRLS HELAETTREK IQAAEYGLAV LEEKHQLKLQ 70 80 90 100 110 120 FEELEVDYEA IRSEMEQLKE AFGQAHTNHK KVAADGESRE ESLIQESASK EQYYVRKVLE 130 140 150 160 170 180 LQTELKQLRN VLTNTQSENE RLTSVAQELK EINQNVEIQR GRLRDDIKEY KFREARLLQD 190 200 210 220 230 240 YSELEEENIS LQKQVSVLRQ NQVEFEGLKH EIKRLEEETE YLNSQLEDAI RLKEISERQL 250 260 270 280 290 300 EEALETLKTE REQKNNLRKE LSHYMSINDS FYTSHLQVSL DGLKFSDDTV TAEPNNDAEA 310 320 330 LVNGFEHSGL VKSSLDNKTS TPRKDGLVHP LHALCLTV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mammalian Golgi-associated Bicaudal-D2 functions in the dynein-dynactin pathway by interacting with these complexes." Hoogenraad C.C., Akhmanova A., Howell S.A., Dortland B.R., de Zeeuw C.I., Willemsen R., Visser P., Grosveld F., Galjart N. EMBO J. 20:4041-4054(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION.
[2]	"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H. DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Mammary tumor.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Strain: C57BL/6J. Tissue: Embryo and Kidney.
[5]	"Bicaudal-D regulates COPI-independent Golgi-ER transport by recruiting the dynein-dynactin motor complex." Matanis T., Akhmanova A., Wulf P., Del Nery E., Weide T., Stepanova T., Galjart N., Grosveld F., Goud B., De Zeeuw C.I., Barnekow A., Hoogenraad C.C. Nat. Cell Biol. 4:986-992(2002) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ250106 mRNA. Translation: CAC51393.1. AK122348 mRNA. Translation: BAC65630.1. Different initiation. BC032198 mRNA. Translation: AAH32198.1. AK002683 mRNA. Translation: BAB22282.1. Frameshift. AK003456 mRNA. Translation: BAC25035.1.
IPI	IPI00267492. IPI00274647. IPI00338578.
RefSeq	NP_001034268.1. NM_001039179.1. NP_001034269.1. NM_001039180.1. NP_084067.1. NM_029791.3.
UniGene	Mm.197387.
3D structure databases
ProteinModelPortal	Q921C5.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-49453N.
IntAct	Q921C5. 1 interaction.
PTM databases
PhosphoSite	Q921C5.
Proteomic databases
PaxDb	Q921C5.
PRIDE	Q921C5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000048544; ENSMUSP00000039394; ENSMUSG00000037933. ENSMUST00000110085; ENSMUSP00000105712; ENSMUSG00000037933.
GeneID	76895.
KEGG	mmu:76895.
UCSC	uc007qjg.1. mouse. uc007qji.1. mouse.
Organism-specific databases
CTD	23299.
MGI	MGI:1924145. Bicd2.
Rouge	Search...
Phylogenomic databases
eggNOG	NOG291306.
GeneTree	ENSGT00390000004114.
HOGENOM	HOG000261658.
HOVERGEN	HBG050686.
OMA	SHTCACA.
OrthoDB	EOG4G4GPX.
Gene expression databases
ArrayExpress	Q921C5.
Bgee	Q921C5.
CleanEx	MM_BICD2.
Genevestigator	Q921C5.
GermOnline	ENSMUSG00000037933. Mus musculus.
Family and domain databases
InterPro	IPR018477. Bicaudal-D_microtubule-assoc. [Graphical view]
PANTHER	PTHR31233. PTHR31233. 1 hit.
Pfam	PF09730. BicD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	346027.
SOURCE	Search...

Entry information

Entry name

BICD2_MOUSE

Accession

Primary (citable) accession number: Q921C5
Secondary accession number(s): Q80TU1, Q8BTE3, Q9DCL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program