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Protein

Bromodomain and WD repeat-containing protein 1

Gene

Brwd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be a transcriptional activator. May be involved in chromatin remodeling. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-1266695. Interleukin-7 signaling.
R-MMU-3247509. Chromatin modifying enzymes.

Names & Taxonomyi

Protein namesi
Recommended name:
Bromodomain and WD repeat-containing protein 1
Alternative name(s):
WD repeat-containing protein 9
Gene namesi
Name:Brwd1
Synonyms:Wdr9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1890651. Brwd1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleolus Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002359861 – 2304Bromodomain and WD repeat-containing protein 1Add BLAST2304

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei687PhosphothreonineCombined sources1
Modified residuei697PhosphoserineCombined sources1
Modified residuei702PhosphoserineBy similarity1
Modified residuei711PhosphoserineBy similarity1
Modified residuei1291PhosphoserineBy similarity1
Modified residuei1481PhosphoserineBy similarity1
Modified residuei1609PhosphoserineCombined sources1
Modified residuei1611PhosphoserineCombined sources1
Modified residuei1682PhosphoserineBy similarity1
Modified residuei1686PhosphoserineBy similarity1
Modified residuei1689PhosphoserineBy similarity1
Modified residuei1751PhosphoserineCombined sources1
Modified residuei1752PhosphoserineCombined sources1
Modified residuei1782PhosphoserineCombined sources1
Modified residuei1784PhosphoserineCombined sources1
Modified residuei1789PhosphoserineCombined sources1
Modified residuei1810PhosphoserineCombined sources1
Modified residuei1830PhosphoserineCombined sources1
Modified residuei1853PhosphothreonineCombined sources1
Modified residuei1857PhosphoserineCombined sources1
Modified residuei1889PhosphoserineBy similarity1
Modified residuei1890PhosphoserineBy similarity1
Modified residuei1927PhosphoserineBy similarity1
Modified residuei1939PhosphothreonineCombined sources1
Modified residuei2004PhosphoserineBy similarity1
Modified residuei2040PhosphoserineCombined sources1
Modified residuei2151PhosphothreonineCombined sources1
Modified residuei2153PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ921C3.
PRIDEiQ921C3.

PTM databases

iPTMnetiQ921C3.
PhosphoSitePlusiQ921C3.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Developmental stagei

Broadly expressed during development. Expression begins to increase at 7.5 dpc, peaks at 10.5-11.5 dpc, and decreases from 14.5 dpc. Weakly expressed at late embryonic stages.1 Publication

Gene expression databases

BgeeiENSMUSG00000022914.
CleanExiMM_BRWD1.
ExpressionAtlasiQ921C3. baseline and differential.
GenevisibleiQ921C3. MM.

Interactioni

Subunit structurei

Interacts with SMARCA4.1 Publication

Protein-protein interaction databases

BioGridi220337. 1 interactor.
IntActiQ921C3. 2 interactors.
STRINGi10090.ENSMUSP00000097101.

Structurei

3D structure databases

ProteinModelPortaliQ921C3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati184 – 223WD 1Add BLAST40
Repeati226 – 265WD 2Add BLAST40
Repeati268 – 311WD 3Add BLAST44
Repeati322 – 365WD 4Add BLAST44
Repeati366 – 405WD 5Add BLAST40
Repeati424 – 463WD 6Add BLAST40
Repeati466 – 506WD 7Add BLAST41
Repeati514 – 553WD 8Add BLAST40
Domaini1178 – 1248Bromo 1PROSITE-ProRule annotationAdd BLAST71
Domaini1332 – 1402Bromo 2PROSITE-ProRule annotationAdd BLAST71

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi661 – 668Poly-Gln8
Compositional biasi817 – 867Ser-richAdd BLAST51
Compositional biasi1454 – 1457Poly-Arg4
Compositional biasi1479 – 1568Ser-richAdd BLAST90

Sequence similaritiesi

Contains 2 bromo domains.PROSITE-ProRule annotation
Contains 8 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Bromodomain, Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410IRXR. Eukaryota.
COG2319. LUCA.
GeneTreeiENSGT00720000108687.
HOGENOMiHOG000095265.
HOVERGENiHBG080933.
InParanoidiQ921C3.
KOiK11798.
TreeFamiTF324197.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
2.130.10.10. 2 hits.
InterProiIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00439. Bromodomain. 2 hits.
PF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 2 hits.
SM00320. WD40. 8 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 2 hits.
SSF50978. SSF50978. 1 hit.
PROSITEiPS00633. BROMODOMAIN_1. 1 hit.
PS50014. BROMODOMAIN_2. 2 hits.
PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q921C3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEPSPARRP VPLIESELYF LIARYLSAGP CRRAAQVLVQ ELEQYQLLPK
60 70 80 90 100
RLDWEGNEHN RSYEELVLSN KHVAPDHLLQ ICQRIGPMLD KEVPPSISRV
110 120 130 140 150
TSLLGAGRQS LLRTAKDCRH TVWKGSAFAA LHRGRPPEMP VNYGPPPSLV
160 170 180 190 200
EIHRGRQLTG CSTFSTAFPG TMYQHIKMHR RILGHLSAVY CVAFDRTGHR
210 220 230 240 250
IFTGSDDCLV KIWSTHNGRL LSTLRGHSAE ISDMAVNYEN TLIAAGSCDK
260 270 280 290 300
IIRVWCLRTC APVAVLQGHT GSITSLQFSP MAKGPQRYMV STGADGTVCF
310 320 330 340 350
WQWDLESLKF SPRPLKFTEK PRPGVQMLCS SFSVGGMFLA TGSTDHVIRM
360 370 380 390 400
YFLGFDAPEK IAELESHTDK VDSIQFCNNG DRFLSGSRDG TARIWRFEQL
410 420 430 440 450
EWRSILLDMS ARISGDTSSE EERFMKPKVT MIAWNQDDST VVTAVNDHVL
460 470 480 490 500
KVWSSYTGQL LHNLLGHADE VFVLETHPFD SRIMLSAGHD GSIFIWDITK
510 520 530 540 550
GIKMKHYFNM IEGQGHGAVF DCKFSQDGQH FACTDSHGHL LIFGFGCSKP
560 570 580 590 600
YEKIPDQMFF HTDYRPLIRD SNNYVLDEQT QQAPHLMPPP FLVDVDGNPH
610 620 630 640 650
PTKFQRLVPG RENSADEHLV PQLGYVATSD GEVIEQIISL HTNDSGDASP
660 670 680 690 700
ESSVLDGMIR QLQQQQDQRL GVDQDGTANG LPSGEGTPRR GSFRRLSLDI
710 720 730 740 750
QSPPNIGLRR SGQVEGVRQM HQNAPRSQIA TERDLQAWKR RVVVPEAPPG
760 770 780 790 800
MFRRLEDIRI ERGEEERNLY VIGRKKKTFQ VPQKSNSMVL VSQSRQRTCR
810 820 830 840 850
RKYANYSRRN ADQCELSSGN ESSGSVRHET SYDQSEGSCS SEDDEWRNDR
860 870 880 890 900
RSGSDSDSSS DSSSQYSDWT ADTGINLQPP LRMSCRRRVT RFCSTSEDEM
910 920 930 940 950
SMENISPPKR RRKRRKESKP KRESLRRMTP AELANMEHLY EFHPPVWITD
960 970 980 990 1000
TTLRKSPFVP QMGDEVMYFR QGHEAYIEAV RRNNIYELNP HKEPWRKMDL
1010 1020 1030 1040 1050
RDQELVKIVG LRYEVGPPTL CCLKLAFIDP ATGRLTDKSF SIRYHDMPDV
1060 1070 1080 1090 1100
IDFLVLRQFY DEARQRNWQP CDRFRSIIDD AWWFGTVLSQ EPYQPQYPDS
1110 1120 1130 1140 1150
HFQCYIVRWD NTETEKLSPW DMEPIPDNVD PPEELGASIS VTSDELEKLL
1160 1170 1180 1190 1200
YKPQEGEWGQ RSRDEECDRI ISGIDQLLNL DIAAAFAGPV DLCTYPKYCT
1210 1220 1230 1240 1250
VVAYPTDLYT IRMRLVNRFY RRLSALIWEV RYIEHNARTF NEPESVIARS
1260 1270 1280 1290 1300
AKKITDQLLK FIKNQDCTNI SELCNTSDND EDDSADDLDD SDLPKTSSGR
1310 1320 1330 1340 1350
RKVHNWKRRS RASGCIESDW RRQCKALLIL IFQCEDSEPF RQPVDLDEYP
1360 1370 1380 1390 1400
DYRDIIDTPM DFGTVRETLE AGNYDSPVEF CKDIRLIFSN AKAYTPNKRS
1410 1420 1430 1440 1450
KIYSMTLRLS ALFEEKMKKI SFDFKIGQKF KEKLRRSQRF KQRQNCNGAV
1460 1470 1480 1490 1500
PGDRRRRNVK QKQFKSQTKV IPQLMCPPSQ STSSKVPLSA TRKTSAGVSS
1510 1520 1530 1540 1550
GFTSGDSSDS AGSLERVRRQ RPEVLRSGSV LFGSEMEDFL ATSSSSSASN
1560 1570 1580 1590 1600
SSEESKASPG ARESSLRSGV LRGSNLGVTR TRAARRKAGS VSLENGCGRK
1610 1620 1630 1640 1650
ATRKRVYLSD SDNNSVETDE NLKNRKCGSS RKVLRKCAAV AASKIKLMSD
1660 1670 1680 1690 1700
AEDSSSESPC SGRKLPHRNA SAVARKKLLH NSDDQSLKSE TEELKDQNQS
1710 1720 1730 1740 1750
LLISGPHSVH NSISDSESDS DLRATRKTWN ANGCTSHTAA TCKTKSRPIE
1760 1770 1780 1790 1800
SSEEDSRCHG SDHGPSSTGD PSTSGQKLRA DSISEEADSE PESSVLCKNT
1810 1820 1830 1840 1850
HLCKKAKILS DSEDCEEKCG ERRGPEVEGS PVSEALREAI LAPQCLSHRG
1860 1870 1880 1890 1900
SETDVDSDGG AVREKSYSNE NGSVSLENGQ RRKVSRKSSS DKESNLQVTQ
1910 1920 1930 1940 1950
KSPKDRSSPS RITQRASVAT DKMKLTSDAE DLSLESVCTR SKRRRKKPAR
1960 1970 1980 1990 2000
FACTPAKTAL SSEEKHAHCE VPEAQPACRN KLPEPEHQDS AENPSQAASA
2010 2020 2030 2040 2050
DLNSGGGSSF EQRKSIQSRQ MGAVCVRPPP KTQSSSAGLS QENARSQTLD
2060 2070 2080 2090 2100
SETSLPSESV LTQKATVESN FEEELNYGLR RWNGRRLRTY GKAPLSRTAQ
2110 2120 2130 2140 2150
VTPSLQASAE VGVKRRRMHP EVDGEDVPGQ MGSSGCGPDT SPKASDLGSV
2160 2170 2180 2190 2200
TDSDVDCTDN TQTQRKKKRK GKARVLSKES VRKDREPHTK MRPCMLNEKD
2210 2220 2230 2240 2250
AVQMHSDTLK AKTVPEKVPR RCATVAANKI KMMCNLKEVV SGPENVGIRT
2260 2270 2280 2290 2300
ISRKLPYRQA SAAAKKKLLS VYKEDDAPIH SENEKELYLR RFRSRKEKAQ

PTPR
Length:2,304
Mass (Da):259,228
Last modified:July 27, 2011 - v2
Checksum:iEC86E1A680C1060E
GO
Isoform B (identifier: Q921C3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2179-2294: ESVRKDREPH...KELYLRRFRS → GKPSSSNSPK...LELTDRTLRP
     2295-2304: Missing.

Show »
Length:2,259
Mass (Da):253,957
Checksum:i307FBDC8266D4B58
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti97 – 103ISRVTSL → KERGTSR in CAC44373 (Ref. 1) Curated7
Sequence conflicti97 – 103ISRVTSL → KERGTSR in CAC44374 (Ref. 1) Curated7
Sequence conflicti251I → V in CAC44373 (Ref. 1) Curated1
Sequence conflicti251I → V in CAC44374 (Ref. 1) Curated1
Sequence conflicti967M → I in CAC44373 (Ref. 1) Curated1
Sequence conflicti967M → I in CAC44374 (Ref. 1) Curated1
Sequence conflicti970R → P in CAC44373 (Ref. 1) Curated1
Sequence conflicti970R → P in CAC44374 (Ref. 1) Curated1
Sequence conflicti996R → G in CAC44373 (Ref. 1) Curated1
Sequence conflicti996R → G in CAC44374 (Ref. 1) Curated1
Sequence conflicti1021C → L in CAC44373 (Ref. 1) Curated1
Sequence conflicti1021C → L in CAC44374 (Ref. 1) Curated1
Sequence conflicti1053F → L in CAC44373 (Ref. 1) Curated1
Sequence conflicti1053F → L in CAC44374 (Ref. 1) Curated1
Sequence conflicti1058Q → P in CAC44373 (Ref. 1) Curated1
Sequence conflicti1058Q → P in CAC44374 (Ref. 1) Curated1
Sequence conflicti1091E → D in CAC44373 (Ref. 1) Curated1
Sequence conflicti1091E → D in CAC44374 (Ref. 1) Curated1
Sequence conflicti1133E → K in CAC44373 (Ref. 1) Curated1
Sequence conflicti1133E → K in CAC44374 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0185522179 – 2294ESVRK…RRFRS → GKPSSSNSPKTARHPRQSKG PRLNVDDNDWEDLDYAKAKG VVRLSKIQTRNQGRRTVRYH DGEDDRSIESALELTDRTLR P in isoform B. 1 PublicationAdd BLAST116
Alternative sequenceiVSP_0185532295 – 2304Missing in isoform B. 1 Publication10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ292467 mRNA. Translation: CAC44373.1.
AJ292468 mRNA. Translation: CAC44374.1.
AC144797 Genomic DNA. No translation available.
AC152502 Genomic DNA. No translation available.
CCDSiCCDS49923.1. [Q921C3-1]
RefSeqiNP_001096649.1. NM_001103179.1. [Q921C3-2]
NP_660107.2. NM_145125.3. [Q921C3-1]
UniGeneiMm.240871.
Mm.482469.

Genome annotation databases

EnsembliENSMUST00000023631; ENSMUSP00000023631; ENSMUSG00000022914. [Q921C3-1]
ENSMUST00000099502; ENSMUSP00000097101; ENSMUSG00000022914. [Q921C3-1]
ENSMUST00000113829; ENSMUSP00000109460; ENSMUSG00000022914. [Q921C3-2]
GeneIDi93871.
KEGGimmu:93871.
UCSCiuc008aci.2. mouse. [Q921C3-1]
uc008ack.2. mouse. [Q921C3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ292467 mRNA. Translation: CAC44373.1.
AJ292468 mRNA. Translation: CAC44374.1.
AC144797 Genomic DNA. No translation available.
AC152502 Genomic DNA. No translation available.
CCDSiCCDS49923.1. [Q921C3-1]
RefSeqiNP_001096649.1. NM_001103179.1. [Q921C3-2]
NP_660107.2. NM_145125.3. [Q921C3-1]
UniGeneiMm.240871.
Mm.482469.

3D structure databases

ProteinModelPortaliQ921C3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi220337. 1 interactor.
IntActiQ921C3. 2 interactors.
STRINGi10090.ENSMUSP00000097101.

PTM databases

iPTMnetiQ921C3.
PhosphoSitePlusiQ921C3.

Proteomic databases

PaxDbiQ921C3.
PRIDEiQ921C3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023631; ENSMUSP00000023631; ENSMUSG00000022914. [Q921C3-1]
ENSMUST00000099502; ENSMUSP00000097101; ENSMUSG00000022914. [Q921C3-1]
ENSMUST00000113829; ENSMUSP00000109460; ENSMUSG00000022914. [Q921C3-2]
GeneIDi93871.
KEGGimmu:93871.
UCSCiuc008aci.2. mouse. [Q921C3-1]
uc008ack.2. mouse. [Q921C3-2]

Organism-specific databases

CTDi54014.
MGIiMGI:1890651. Brwd1.

Phylogenomic databases

eggNOGiENOG410IRXR. Eukaryota.
COG2319. LUCA.
GeneTreeiENSGT00720000108687.
HOGENOMiHOG000095265.
HOVERGENiHBG080933.
InParanoidiQ921C3.
KOiK11798.
TreeFamiTF324197.

Enzyme and pathway databases

ReactomeiR-MMU-1266695. Interleukin-7 signaling.
R-MMU-3247509. Chromatin modifying enzymes.

Miscellaneous databases

ChiTaRSiBrwd1. mouse.
PROiQ921C3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022914.
CleanExiMM_BRWD1.
ExpressionAtlasiQ921C3. baseline and differential.
GenevisibleiQ921C3. MM.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
2.130.10.10. 2 hits.
InterProiIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00439. Bromodomain. 2 hits.
PF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 2 hits.
SM00320. WD40. 8 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 2 hits.
SSF50978. SSF50978. 1 hit.
PROSITEiPS00633. BROMODOMAIN_1. 1 hit.
PS50014. BROMODOMAIN_2. 2 hits.
PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBRWD1_MOUSE
AccessioniPrimary (citable) accession number: Q921C3
Secondary accession number(s): E9QAC1, Q921C2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.