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Protein

CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase

Gene

ST8SIA4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid (PSA), which is present on the embryonic neural cell adhesion molecule (N-CAM), necessary for plasticity of neural cells.

Pathway: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Source: BHF-UCL
  • sialic acid binding Source: BHF-UCL

GO - Biological processi

  • axon guidance Source: Reactome
  • cellular protein metabolic process Source: Reactome
  • cellular protein modification process Source: ProtInc
  • dolichol-linked oligosaccharide biosynthetic process Source: Reactome
  • ganglioside biosynthetic process Source: BHF-UCL
  • nervous system development Source: ProtInc
  • N-glycan processing Source: BHF-UCL
  • oligosaccharide metabolic process Source: BHF-UCL
  • post-translational protein modification Source: Reactome
  • protein glycosylation Source: BHF-UCL
  • protein N-linked glycosylation via asparagine Source: Reactome
  • sialylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.99.8. 2681.
ReactomeiREACT_18312. NCAM1 interactions.
REACT_264366. Sialic acid metabolism.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase (EC:2.4.99.-)
Alternative name(s):
Alpha-2,8-sialyltransferase 8D
Polysialyltransferase-1
Sialyltransferase 8D
Short name:
SIAT8-D
Sialyltransferase St8Sia IV
Short name:
ST8SiaIV
Gene namesi
Name:ST8SIA4
Synonyms:PST, PST1, SIAT8D
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:10871. ST8SIA4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence Analysis
Transmembranei8 – 2013Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini21 – 359339LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35772.

Polymorphism and mutation databases

DMDMi2494834.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferasePRO_0000149293Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi50 – 501N-linked (GlcNAc...)Sequence Analysis
Glycosylationi74 – 741N-linked (GlcNAc...)1 Publication
Glycosylationi119 – 1191N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi142 ↔ 2921 Publication
Disulfide bondi156 ↔ 3561 Publication
Glycosylationi204 – 2041N-linked (GlcNAc...)Sequence Analysis
Glycosylationi219 – 2191N-linked (GlcNAc...)Sequence Analysis
Glycosylationi327 – 3271N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ92187.
PRIDEiQ92187.

PTM databases

PhosphoSiteiQ92187.

Expressioni

Tissue specificityi

Highly expressed in fetal brain, lung and kidney and in adult heart, spleen and thymus. Present to a lesser extent in adult brain, placenta, lung, large and small intestine and peripheral blood leukocytes.

Gene expression databases

BgeeiQ92187.
CleanExiHS_ST8SIA4.
ExpressionAtlasiQ92187. baseline and differential.
GenevisibleiQ92187. HS.

Organism-specific databases

HPAiHPA051126.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000231461.

Structurei

3D structure databases

ProteinModelPortaliQ92187.
SMRiQ92187. Positions 123-318.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG324948.
GeneTreeiENSGT00550000074407.
HOGENOMiHOG000207604.
HOVERGENiHBG056466.
InParanoidiQ92187.
KOiK06614.
OMAiSVRKRWT.
OrthoDBiEOG7VQJD1.
PhylomeDBiQ92187.
TreeFamiTF352820.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q92187-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRSIRKRWTI CTISLLLIFY KTKEIARTEE HQETQLIGDG ELSLSRSLVN
60 70 80 90 100
SSDKIIRKAG SSIFQHNVEG WKINSSLVLE IRKNILRFLD AERDVSVVKS
110 120 130 140 150
SFKPGDVIHY VLDRRRTLNI SHDLHSLLPE VSPMKNRRFK TCAVVGNSGI
160 170 180 190 200
LLDSECGKEI DSHNFVIRCN LAPVVEFAAD VGTKSDFITM NPSVVQRAFG
210 220 230 240 250
GFRNESDREK FVHRLSMLND SVLWIPAFMV KGGEKHVEWV NALILKNKLK
260 270 280 290 300
VRTAYPSLRL IHAVRGYWLT NKVPIKRPST GLLMYTLATR FCDEIHLYGF
310 320 330 340 350
WPFPKDLNGK AVKYHYYDDL KYRYFSNASP HRMPLEFKTL NVLHNRGALK

LTTGKCVKQ
Length:359
Mass (Da):41,295
Last modified:February 1, 1997 - v1
Checksum:iC53FFDF658926D61
GO
Isoform 2 (identifier: Q92187-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     169-359: Missing.

Note: No experimental confirmation available.
Show »
Length:168
Mass (Da):19,260
Checksum:i2B9ED426B1A3C9F2
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti7 – 71R → K.1 Publication
Corresponds to variant rs199768560 [ dbSNP | Ensembl ].
VAR_068976
Natural varianti92 – 921E → G in a colorectal cancer sample; somatic mutation. 1 Publication
VAR_036169

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei169 – 359191Missing in isoform 2. 1 PublicationVSP_044867Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41680 mRNA. Translation: AAC41775.1.
AK292872 mRNA. Translation: BAF85561.1.
AC010411 Genomic DNA. No translation available.
AC117530 Genomic DNA. No translation available.
CH471086 Genomic DNA. Translation: EAW49104.1.
CH471086 Genomic DNA. Translation: EAW49105.1.
BC027866 mRNA. Translation: AAH27866.1.
BC053657 mRNA. Translation: AAH53657.1.
CCDSiCCDS4091.1. [Q92187-1]
CCDS47252.1. [Q92187-2]
PIRiI59403.
RefSeqiNP_005659.1. NM_005668.5. [Q92187-1]
NP_778222.1. NM_175052.2. [Q92187-2]
UniGeneiHs.308628.

Genome annotation databases

EnsembliENST00000231461; ENSP00000231461; ENSG00000113532. [Q92187-1]
ENST00000451528; ENSP00000428914; ENSG00000113532. [Q92187-2]
GeneIDi7903.
KEGGihsa:7903.
UCSCiuc003knk.3. human. [Q92187-1]
uc003knl.3. human.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

ST8Sia IV

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L41680 mRNA. Translation: AAC41775.1.
AK292872 mRNA. Translation: BAF85561.1.
AC010411 Genomic DNA. No translation available.
AC117530 Genomic DNA. No translation available.
CH471086 Genomic DNA. Translation: EAW49104.1.
CH471086 Genomic DNA. Translation: EAW49105.1.
BC027866 mRNA. Translation: AAH27866.1.
BC053657 mRNA. Translation: AAH53657.1.
CCDSiCCDS4091.1. [Q92187-1]
CCDS47252.1. [Q92187-2]
PIRiI59403.
RefSeqiNP_005659.1. NM_005668.5. [Q92187-1]
NP_778222.1. NM_175052.2. [Q92187-2]
UniGeneiHs.308628.

3D structure databases

ProteinModelPortaliQ92187.
SMRiQ92187. Positions 123-318.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000231461.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

PTM databases

PhosphoSiteiQ92187.

Polymorphism and mutation databases

DMDMi2494834.

Proteomic databases

PaxDbiQ92187.
PRIDEiQ92187.

Protocols and materials databases

DNASUi7903.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000231461; ENSP00000231461; ENSG00000113532. [Q92187-1]
ENST00000451528; ENSP00000428914; ENSG00000113532. [Q92187-2]
GeneIDi7903.
KEGGihsa:7903.
UCSCiuc003knk.3. human. [Q92187-1]
uc003knl.3. human.

Organism-specific databases

CTDi7903.
GeneCardsiGC05M100171.
HGNCiHGNC:10871. ST8SIA4.
HPAiHPA051126.
MIMi602547. gene.
neXtProtiNX_Q92187.
PharmGKBiPA35772.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG324948.
GeneTreeiENSGT00550000074407.
HOGENOMiHOG000207604.
HOVERGENiHBG056466.
InParanoidiQ92187.
KOiK06614.
OMAiSVRKRWT.
OrthoDBiEOG7VQJD1.
PhylomeDBiQ92187.
TreeFamiTF352820.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.99.8. 2681.
ReactomeiREACT_18312. NCAM1 interactions.
REACT_264366. Sialic acid metabolism.

Miscellaneous databases

ChiTaRSiST8SIA4. human.
GeneWikiiST8SIA4.
GenomeRNAii7903.
NextBioi30362.
PROiQ92187.
SOURCEiSearch...

Gene expression databases

BgeeiQ92187.
CleanExiHS_ST8SIA4.
ExpressionAtlasiQ92187. baseline and differential.
GenevisibleiQ92187. HS.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression cloning of a human polysialyltransferase that forms the polysialylated neural cell adhesion molecule present in embryonic brain."
    Nakayama J., Fukuda M.N., Fredette B., Ranscht B., Fukuda M.
    Proc. Natl. Acad. Sci. U.S.A. 92:7031-7035(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Fetal brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Trachea.
  3. "The DNA sequence and comparative analysis of human chromosome 5."
    Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
    , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
    Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT LYS-7.
    Tissue: Brain and Lung.
  6. "Unique disulfide bond structures found in ST8Sia IV polysialyltransferase are required for its activity."
    Angata K., Yen T.Y., El-Battari A., Macher B.A., Fukuda M.
    J. Biol. Chem. 276:15369-15377(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS, GLYCOSYLATION AT ASN-74.
  7. Cited for: VARIANT [LARGE SCALE ANALYSIS] GLY-92.

Entry informationi

Entry nameiSIA8D_HUMAN
AccessioniPrimary (citable) accession number: Q92187
Secondary accession number(s): A8KA07
, G3V104, Q8N1F4, Q92693
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 24, 2015
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.