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Protein

Alpha-2,8-sialyltransferase 8B

Gene

ST8SIA2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May transfer sialic acid through alpha-2,8-linkages to the alpha-2,3-linked and alpha-2,6-linked sialic acid of N-linked oligosaccharides of glycoproteins and may be involved in PSA (polysialic acid) expression.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei185 – 1851SubstrateBy similarity
Active sitei346 – 3461Proton donor/acceptorBy similarity

GO - Molecular functioni

  • alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Source: BHF-UCL
  • sialic acid binding Source: BHF-UCL

GO - Biological processi

  • carbohydrate metabolic process Source: ProtInc
  • cellular protein modification process Source: ProtInc
  • ganglioside biosynthetic process Source: BHF-UCL
  • nervous system development Source: ProtInc
  • N-glycan processing Source: BHF-UCL
  • oligosaccharide metabolic process Source: BHF-UCL
  • protein glycosylation Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-HSA-4085001. Sialic acid metabolism.
R-HSA-419037. NCAM1 interactions.
R-HSA-975577. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2,8-sialyltransferase 8B (EC:2.4.99.-)
Alternative name(s):
Sialyltransferase 8B
Short name:
SIAT8-B
Sialyltransferase St8Sia II
Short name:
ST8SiaII
Sialyltransferase X
Short name:
STX
Gene namesi
Name:ST8SIA2
Synonyms:SIAT8B, STX
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:10870. ST8SIA2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence analysis
Transmembranei7 – 2317Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini24 – 375352LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35771.

Polymorphism and mutation databases

BioMutaiST8SIA2.
DMDMi2494831.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 375375Alpha-2,8-sialyltransferase 8BPRO_0000149285Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi60 – 601N-linked (GlcNAc...)Sequence analysis
Glycosylationi72 – 721N-linked (GlcNAc...)Sequence analysis
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence analysis
Glycosylationi134 – 1341N-linked (GlcNAc...)Sequence analysis
Disulfide bondi157 ↔ 307By similarity
Disulfide bondi171 ↔ 371By similarity
Glycosylationi219 – 2191N-linked (GlcNAc...)Sequence analysis
Glycosylationi234 – 2341N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ92186.
PeptideAtlasiQ92186.
PRIDEiQ92186.

PTM databases

iPTMnetiQ92186.
PhosphoSiteiQ92186.

Expressioni

Tissue specificityi

Highly expressed in fetal brain, kidney and heart and to a much lesser extent in adult heart and thymus.

Gene expression databases

BgeeiQ92186.
CleanExiHS_ST8SIA2.
ExpressionAtlasiQ92186. baseline and differential.
GenevisibleiQ92186. HS.

Organism-specific databases

HPAiHPA054518.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000268164.

Structurei

3D structure databases

ProteinModelPortaliQ92186.
SMRiQ92186. Positions 137-315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni206 – 2083Substrate bindingBy similarity
Regioni294 – 2963Substrate bindingBy similarity
Regioni329 – 3302Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074407.
HOGENOMiHOG000232070.
HOVERGENiHBG056466.
InParanoidiQ92186.
KOiK06612.
OMAiIHYVFDR.
OrthoDBiEOG7VQJD1.
PhylomeDBiQ92186.
TreeFamiTF352820.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequencei

Sequence statusi: Complete.

Q92186-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLQFRSWML AALTLLVVFL IFADISEIEE EIGNSGGRGT IRSAVNSLHS
60 70 80 90 100
KSNRAEVVIN GSSSPAVVDR SNESIKHNIQ PASSKWRHNQ TLSLRIRKQI
110 120 130 140 150
LKFLDAEKDI SVLKGTLKPG DIIHYIFDRD STMNVSQNLY ELLPRTSPLK
160 170 180 190 200
NKHFGTCAIV GNSGVLLNSG CGQEIDAHSF VIRCNLAPVQ EYARDVGLKT
210 220 230 240 250
DLVTMNPSVI QRAFEDLVNA TWREKLLQRL HSLNGSILWI PAFMARGGKE
260 270 280 290 300
RVEWVNELIL KHHVNVRTAY PSLRLLHAVR GYWLTNKVHI KRPTTGLLMY
310 320 330 340 350
TLATRFCKQI YLYGFWPFPL DQNQNPVKYH YYDSLKYGYT SQASPHTMPL
360 370
EFKALKSLHE QGALKLTVGQ CDGAT
Length:375
Mass (Da):42,430
Last modified:February 1, 1997 - v1
Checksum:i42F0BC5B33D62325
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti155 – 1551G → Q in AAA36613 (PubMed:9054414).Curated
Sequence conflicti171 – 1711C → Y in AAA36613 (PubMed:9054414).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33551 mRNA. Translation: AAC24458.1.
U82762 mRNA. Translation: AAB51242.1.
BC069584 mRNA. Translation: AAH69584.1.
BC096203 mRNA. Translation: AAH96203.1.
BC096204 mRNA. Translation: AAH96204.1.
L29556 mRNA. Translation: AAA36613.1.
CCDSiCCDS10372.1.
PIRiB54898.
I39169.
RefSeqiNP_006002.1. NM_006011.3.
UniGeneiHs.302341.

Genome annotation databases

EnsembliENST00000268164; ENSP00000268164; ENSG00000140557.
GeneIDi8128.
KEGGihsa:8128.
UCSCiuc002bra.4. human.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST8Sia II

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33551 mRNA. Translation: AAC24458.1.
U82762 mRNA. Translation: AAB51242.1.
BC069584 mRNA. Translation: AAH69584.1.
BC096203 mRNA. Translation: AAH96203.1.
BC096204 mRNA. Translation: AAH96204.1.
L29556 mRNA. Translation: AAA36613.1.
CCDSiCCDS10372.1.
PIRiB54898.
I39169.
RefSeqiNP_006002.1. NM_006011.3.
UniGeneiHs.302341.

3D structure databases

ProteinModelPortaliQ92186.
SMRiQ92186. Positions 137-315.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000268164.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

PTM databases

iPTMnetiQ92186.
PhosphoSiteiQ92186.

Polymorphism and mutation databases

BioMutaiST8SIA2.
DMDMi2494831.

Proteomic databases

PaxDbiQ92186.
PeptideAtlasiQ92186.
PRIDEiQ92186.

Protocols and materials databases

DNASUi8128.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000268164; ENSP00000268164; ENSG00000140557.
GeneIDi8128.
KEGGihsa:8128.
UCSCiuc002bra.4. human.

Organism-specific databases

CTDi8128.
GeneCardsiST8SIA2.
HGNCiHGNC:10870. ST8SIA2.
HPAiHPA054518.
MIMi602546. gene.
neXtProtiNX_Q92186.
PharmGKBiPA35771.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074407.
HOGENOMiHOG000232070.
HOVERGENiHBG056466.
InParanoidiQ92186.
KOiK06612.
OMAiIHYVFDR.
OrthoDBiEOG7VQJD1.
PhylomeDBiQ92186.
TreeFamiTF352820.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-HSA-4085001. Sialic acid metabolism.
R-HSA-419037. NCAM1 interactions.
R-HSA-975577. N-Glycan antennae elongation.

Miscellaneous databases

ChiTaRSiST8SIA2. human.
GeneWikiiST8SIA2.
GenomeRNAii8128.
PROiQ92186.
SOURCEiSearch...

Gene expression databases

BgeeiQ92186.
CleanExiHS_ST8SIA2.
ExpressionAtlasiQ92186. baseline and differential.
GenevisibleiQ92186. HS.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A human STX cDNA confers polysialic acid expression in mammalian cells."
    Scheidegger E.P., Sternberg L.R., Roth J., Lowe J.B.
    J. Biol. Chem. 270:22685-22688(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Human STX polysialyltransferase forms the embryonic form of the neural cell adhesion molecule. Tissue-specific expression, neurite outgrowth, and chromosomal localization in comparison with another polysialyltransferase, PST."
    Angata K., Nakayama J., Fredette B., Chong K., Ranscht B., Fukuda M.
    J. Biol. Chem. 272:7182-7190(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Differential expression of five sialyltransferase genes in human tissues."
    Kitagawa H., Paulson J.C.
    J. Biol. Chem. 269:17872-17878(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 154-375.
    Tissue: Fetal brain.

Entry informationi

Entry nameiSIA8B_HUMAN
AccessioniPrimary (citable) accession number: Q92186
Secondary accession number(s): Q4VAZ0, Q92470, Q92746
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 6, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.