Q92186 (SIA8B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-2,8-sialyltransferase 8B
EC=2.4.99.-

Alternative name(s):
Sialyltransferase 8B
Short name=SIAT8-B
Sialyltransferase X
Short name=STX
Sialytransferase St8Sia II
Short name=ST8SiaII

Gene names

Name:	ST8SIA2
Synonyms:	SIAT8B, STX

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May transfer sialic acid through alpha-2,8-linkages to the alpha-2,3-linked and alpha-2,6-linked sialic acid of N-linked oligosaccharides of glycoproteins and may be involved in PSA (polysialic acid) expression.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Tissue specificity	Highly expressed in fetal brain, kidney and heart and to a much lesser extent in adult heart and thymus.
Sequence similarities	Belongs to the glycosyltransferase 29 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	N-glycan processing Inferred from direct assay. Source: BHF-UCL axon guidance Traceable author statement. Source: Reactome ganglioside biosynthetic process Inferred from direct assay. Source: BHF-UCL post-translational protein modification Traceable author statement. Source: Reactome protein N-linked glycosylation via asparagine Traceable author statement. Source: Reactome
Cellular component	integral to Golgi membrane Inferred from electronic annotation. Source: InterPro
Molecular function	alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Inferred from direct assay. Source: BHF-UCL sialic acid binding Inferred by curator. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Alpha-2,8-sialyltransferase 8B		PRO_0000149285
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 23	17	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	24 – 375	352	Lumenal Potential
Amino acid modifications
	Glycosylation	60	1	N-linked (GlcNAc...) Potential
	Glycosylation	72	1	N-linked (GlcNAc...) Potential
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
	Glycosylation	134	1	N-linked (GlcNAc...) Potential
	Glycosylation	219	1	N-linked (GlcNAc...) Potential
	Glycosylation	234	1	N-linked (GlcNAc...) Potential
	Disulfide bond	157 ↔ 307		By similarity
Experimental info
	Sequence conflict	155	1	G → Q in AAA36613. Ref.2
	Sequence conflict	171	1	C → Y in AAA36613. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q92186 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 42F0BC5B33D62325	FASTA	375	42,430
	10 20 30 40 50 60 MQLQFRSWML AALTLLVVFL IFADISEIEE EIGNSGGRGT IRSAVNSLHS KSNRAEVVIN 70 80 90 100 110 120 GSSSPAVVDR SNESIKHNIQ PASSKWRHNQ TLSLRIRKQI LKFLDAEKDI SVLKGTLKPG 130 140 150 160 170 180 DIIHYIFDRD STMNVSQNLY ELLPRTSPLK NKHFGTCAIV GNSGVLLNSG CGQEIDAHSF 190 200 210 220 230 240 VIRCNLAPVQ EYARDVGLKT DLVTMNPSVI QRAFEDLVNA TWREKLLQRL HSLNGSILWI 250 260 270 280 290 300 PAFMARGGKE RVEWVNELIL KHHVNVRTAY PSLRLLHAVR GYWLTNKVHI KRPTTGLLMY 310 320 330 340 350 360 TLATRFCKQI YLYGFWPFPL DQNQNPVKYH YYDSLKYGYT SQASPHTMPL EFKALKSLHE 370 QGALKLTVGQ CDGAT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A human STX cDNA confers polysialic acid expression in mammalian cells." Scheidegger E.P., Sternberg L.R., Roth J., Lowe J.B. J. Biol. Chem. 270:22685-22688(1995) [PubMed: 7559389] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Human STX polysialyltransferase forms the embryonic form of the neural cell adhesion molecule. Tissue-specific expression, neurite outgrowth, and chromosomal localization in comparison with another polysialyltransferase, PST." Angata K., Nakayama J., Fredette B., Chong K., Ranscht B., Fukuda M. J. Biol. Chem. 272:7182-7190(1997) [PubMed: 9054414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Differential expression of five sialyltransferase genes in human tissues." Kitagawa H., Paulson J.C. J. Biol. Chem. 269:17872-17878(1994) [PubMed: 8027041] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 154-375. Tissue: Fetal brain.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

ST8Sia II

Cross-references

Sequence databases
EMBL GenBank DDBJ	U33551 mRNA. Translation: AAC24458.1. U82762 mRNA. Translation: AAB51242.1. BC069584 mRNA. Translation: AAH69584.1. BC096203 mRNA. Translation: AAH96203.1. BC096204 mRNA. Translation: AAH96204.1. L29556 mRNA. Translation: AAA36613.1.
IPI	IPI00020199.
PIR	B54898. I39169.
RefSeq	NP_006002.1. NM_006011.3.
UniGene	Hs.302341.
3D structure databases
ProteinModelPortal	Q92186.
ModBase	Search...
Protein-protein interaction databases
STRING	Q92186.
Protein family/group databases
CAZy	GT29. Glycosyltransferase Family 29.
Polymorphism databases
DMDM	2494831.
Proteomic databases
PRIDE	Q92186.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000268164; ENSP00000268164; ENSG00000140557.
GeneID	8128.
KEGG	hsa:8128.
UCSC	uc002bra.1. human.
Organism-specific databases
CTD	8128.
GeneCards	GC15P092937.
H-InvDB	HIX0026911.
HGNC	HGNC:10870. ST8SIA2.
MIM	602546. gene.
neXtProt	NX_Q92186.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00550000074407.
HOGENOM	HBG717846.
HOVERGEN	HBG056466.
InParanoid	Q92186.
OMA	NPVKYHY.
OrthoDB	EOG4FTW0Z.
PhylomeDB	Q92186.
Enzyme and pathway databases
Reactome	REACT_111045. Developmental Biology. REACT_17015. Metabolism of proteins.
Gene expression databases
ArrayExpress	Q92186.
Bgee	Q92186.
CleanEx	HS_ST8SIA2.
Genevestigator	Q92186.
GermOnline	ENSG00000140557. Homo sapiens.
Family and domain databases
InterPro	IPR001675. Glyco_trans_29. IPR012163. Sialyl_trans. [Graphical view]
KO	K06612.
Pfam	PF00777. Glyco_transf_29. 1 hit. [Graphical view]
PIRSF	PIRSF005557. Sialyl_trans. 1 hit.
ProtoNet	Search...
Other
NextBio	30791.
SOURCE	Search...

Entry information

Entry name

SIA8B_HUMAN

Accession

Primary (citable) accession number: Q92186
Secondary accession number(s): Q4VAZ0, Q92470, Q92746

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.