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Protein

Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase

Gene

ST8SIA1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the production of gangliosides GD3 and GT3 from GM3; gangliosides are a subfamily of complex glycosphinglolipds that contain one or more residues of sialic acid.4 Publications

Catalytic activityi

CMP-N-acetylneuraminate + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-R = CMP + alpha-N-acetylneuraminyl-(2->8)-alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-R.4 Publications

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei166SubstrateBy similarity1
Active sitei322Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Source: Reactome
  • sialyltransferase activity Source: ProtInc

GO - Biological processi

  • carbohydrate metabolic process Source: ProtInc
  • cellular response to heat Source: Ensembl
  • glycosphingolipid biosynthetic process Source: ProtInc
  • positive regulation of cell proliferation Source: Ensembl
  • protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

BioCyciZFISH:HS03456-MONOMER.
BRENDAi2.4.99.8. 2681.
ReactomeiR-HSA-4085001. Sialic acid metabolism.
UniPathwayiUPA00222.
UPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Chemistry databases

SwissLipidsiSLP:000000749.
SLP:000000871.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase (EC:2.4.99.8)
Alternative name(s):
Alpha-2,8-sialyltransferase 8A
Ganglioside GD3 synthase
Ganglioside GT3 synthase
Sialyltransferase 8A
Short name:
SIAT8-A
Sialyltransferase St8Sia I
Short name:
ST8SiaI
Gene namesi
Name:ST8SIA1
Synonyms:SIAT8, SIAT8A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:10869. ST8SIA1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 29CytoplasmicSequence analysisAdd BLAST29
Transmembranei30 – 48Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST19
Topological domaini49 – 356LumenalSequence analysisAdd BLAST308

GO - Cellular componenti

  • Golgi membrane Source: Reactome
  • integral component of Golgi membrane Source: InterPro
  • integral component of membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi6489.
OpenTargetsiENSG00000111728.
PharmGKBiPA35770.

Polymorphism and mutation databases

BioMutaiST8SIA1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001492821 – 356Alpha-N-acetylneuraminide alpha-2,8-sialyltransferaseAdd BLAST356

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi71N-linked (GlcNAc...)Sequence analysis1
Glycosylationi119N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi138 ↔ 287By similarity
Disulfide bondi152 ↔ 347By similarity
Glycosylationi214N-linked (GlcNAc...)Sequence analysis1
Glycosylationi245N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ92185.
PeptideAtlasiQ92185.
PRIDEiQ92185.

PTM databases

iPTMnetiQ92185.
PhosphoSitePlusiQ92185.

Expressioni

Tissue specificityi

Strongly expressed in melanoma cell lines, adult and fetal brain and to a lesser extent in adult and fetal lung.2 Publications

Gene expression databases

BgeeiENSG00000111728.
CleanExiHS_ST8SIA1.
ExpressionAtlasiQ92185. baseline and differential.
GenevisibleiQ92185. HS.

Organism-specific databases

HPAiHPA026775.

Interactioni

Protein-protein interaction databases

BioGridi112380. 10 interactors.
STRINGi9606.ENSP00000379353.

Structurei

3D structure databases

ProteinModelPortaliQ92185.
SMRiQ92185.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni188 – 190Substrate bindingBy similarity3
Regioni274 – 276Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074407.
HOGENOMiHOG000090201.
HOVERGENiHBG106106.
InParanoidiQ92185.
KOiK03371.
OMAiCWLYVFP.
OrthoDBiEOG091G0CER.
PhylomeDBiQ92185.
TreeFamiTF323961.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q92185-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSPCGRARRQ TSRGAMAVLA WKFPRTRLPM GASALCVVVL CWLYIFPVYR
60 70 80 90 100
LPNEKEIVQG VLQQGTAWRR NQTAARAFRK QMEDCCDPAH LFAMTKMNSP
110 120 130 140 150
MGKSMWYDGE FLYSFTIDNS TYSLFPQATP FQLPLKKCAV VGNGGILKKS
160 170 180 190 200
GCGRQIDEAN FVMRCNLPPL SSEYTKDVGS KSQLVTANPS IIRQRFQNLL
210 220 230 240 250
WSRKTFVDNM KIYNHSYIYM PAFSMKTGTE PSLRVYYTLS DVGANQTVLF
260 270 280 290 300
ANPNFLRSIG KFWKSRGIHA KRLSTGLFLV SAALGLCEEV AIYGFWPFSV
310 320 330 340 350
NMHEQPISHH YYDNVLPFSG FHAMPEEFLQ LWYLHKIGAL RMQLDPCEDT

SLQPTS
Length:356
Mass (Da):40,519
Last modified:February 1, 1997 - v1
Checksum:i452FE04856964395
GO
Isoform 2 (identifier: Q92185-2) [UniParc]FASTAAdd to basket
Also known as: Sat-2

The sequence of this isoform differs from the canonical sequence as follows:
     128-135: ATPFQLPL → MQSPSFVK
     136-356: Missing.

Show »
Length:135
Mass (Da):15,504
Checksum:iC6807FD99973BDDC
GO

Sequence cautioni

The sequence AAC37586 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA54891 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_047583128 – 135ATPFQLPL → MQSPSFVK in isoform 2. 2 Publications8
Alternative sequenceiVSP_047584136 – 356Missing in isoform 2. 2 PublicationsAdd BLAST221

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77922 mRNA. Translation: CAA54891.1. Different initiation.
D26360 mRNA. Translation: BAA05391.1.
L32867 mRNA. Translation: AAA62366.1.
L43494 mRNA. Translation: AAC37586.1. Different initiation.
AY569975 mRNA. Translation: AAS75783.1.
EU041716 mRNA. Translation: ABU62753.1.
AK290941 mRNA. Translation: BAF83630.1.
AK315340 mRNA. Translation: BAG37739.1.
AC007544 Genomic DNA. No translation available.
AC007671 Genomic DNA. No translation available.
AC053513 Genomic DNA. No translation available.
AC087318 Genomic DNA. No translation available.
AC138468 Genomic DNA. No translation available.
CH471094 Genomic DNA. Translation: EAW96468.1.
BC117285 mRNA. Translation: AAI17286.1.
BC126162 mRNA. Translation: AAI26163.1.
CCDSiCCDS8697.1. [Q92185-1]
PIRiA54032.
RefSeqiNP_001291379.1. NM_001304450.1.
NP_003025.1. NM_003034.3. [Q92185-1]
UniGeneiHs.408614.

Genome annotation databases

EnsembliENST00000261197; ENSP00000261197; ENSG00000111728. [Q92185-2]
ENST00000396037; ENSP00000379353; ENSG00000111728. [Q92185-1]
GeneIDi6489.
KEGGihsa:6489.
UCSCiuc001rfo.4. human. [Q92185-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST8Sia I

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77922 mRNA. Translation: CAA54891.1. Different initiation.
D26360 mRNA. Translation: BAA05391.1.
L32867 mRNA. Translation: AAA62366.1.
L43494 mRNA. Translation: AAC37586.1. Different initiation.
AY569975 mRNA. Translation: AAS75783.1.
EU041716 mRNA. Translation: ABU62753.1.
AK290941 mRNA. Translation: BAF83630.1.
AK315340 mRNA. Translation: BAG37739.1.
AC007544 Genomic DNA. No translation available.
AC007671 Genomic DNA. No translation available.
AC053513 Genomic DNA. No translation available.
AC087318 Genomic DNA. No translation available.
AC138468 Genomic DNA. No translation available.
CH471094 Genomic DNA. Translation: EAW96468.1.
BC117285 mRNA. Translation: AAI17286.1.
BC126162 mRNA. Translation: AAI26163.1.
CCDSiCCDS8697.1. [Q92185-1]
PIRiA54032.
RefSeqiNP_001291379.1. NM_001304450.1.
NP_003025.1. NM_003034.3. [Q92185-1]
UniGeneiHs.408614.

3D structure databases

ProteinModelPortaliQ92185.
SMRiQ92185.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112380. 10 interactors.
STRINGi9606.ENSP00000379353.

Chemistry databases

SwissLipidsiSLP:000000749.
SLP:000000871.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

PTM databases

iPTMnetiQ92185.
PhosphoSitePlusiQ92185.

Polymorphism and mutation databases

BioMutaiST8SIA1.

Proteomic databases

PaxDbiQ92185.
PeptideAtlasiQ92185.
PRIDEiQ92185.

Protocols and materials databases

DNASUi6489.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261197; ENSP00000261197; ENSG00000111728. [Q92185-2]
ENST00000396037; ENSP00000379353; ENSG00000111728. [Q92185-1]
GeneIDi6489.
KEGGihsa:6489.
UCSCiuc001rfo.4. human. [Q92185-1]

Organism-specific databases

CTDi6489.
DisGeNETi6489.
GeneCardsiST8SIA1.
HGNCiHGNC:10869. ST8SIA1.
HPAiHPA026775.
MIMi601123. gene.
neXtProtiNX_Q92185.
OpenTargetsiENSG00000111728.
PharmGKBiPA35770.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074407.
HOGENOMiHOG000090201.
HOVERGENiHBG106106.
InParanoidiQ92185.
KOiK03371.
OMAiCWLYVFP.
OrthoDBiEOG091G0CER.
PhylomeDBiQ92185.
TreeFamiTF323961.

Enzyme and pathway databases

UniPathwayiUPA00222.
UPA00378.
BioCyciZFISH:HS03456-MONOMER.
BRENDAi2.4.99.8. 2681.
ReactomeiR-HSA-4085001. Sialic acid metabolism.

Miscellaneous databases

ChiTaRSiST8SIA1. human.
GeneWikiiST8SIA1.
GenomeRNAii6489.
PROiQ92185.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000111728.
CleanExiHS_ST8SIA1.
ExpressionAtlasiQ92185. baseline and differential.
GenevisibleiQ92185. HS.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSIA8A_HUMAN
AccessioniPrimary (citable) accession number: Q92185
Secondary accession number(s): A8K4H6
, Q17RL0, Q6PZN5, Q93064
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.