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Protein

Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase

Gene

ST8SIA1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the production of gangliosides GD3 and GT3 from GM3; gangliosides are a subfamily of complex glycosphinglolipds that contain one or more residues of sialic acid.4 Publications

Catalytic activityi

CMP-N-acetylneuraminate + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-R = CMP + alpha-N-acetylneuraminyl-(2->8)-alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-R.4 Publications

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei166SubstrateBy similarity1
Active sitei322Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Source: Reactome
  • sialyltransferase activity Source: ProtInc

GO - Biological processi

  • carbohydrate metabolic process Source: ProtInc
  • cellular response to heat Source: Ensembl
  • glycosphingolipid biosynthetic process Source: ProtInc
  • positive regulation of cell proliferation Source: Ensembl
  • protein glycosylation Source: UniProtKB-UniPathway

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processLipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:HS03456-MONOMER
BRENDAi2.4.99.8 2681
ReactomeiR-HSA-4085001 Sialic acid metabolism
UniPathwayiUPA00222
UPA00378

Protein family/group databases

CAZyiGT29 Glycosyltransferase Family 29

Chemistry databases

SwissLipidsiSLP:000000749
SLP:000000871

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase (EC:2.4.99.8)
Alternative name(s):
Alpha-2,8-sialyltransferase 8A
Ganglioside GD3 synthase
Ganglioside GT3 synthase
Sialyltransferase 8A
Short name:
SIAT8-A
Sialyltransferase St8Sia I
Short name:
ST8SiaI
Gene namesi
Name:ST8SIA1
Synonyms:SIAT8, SIAT8A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000111728.10
HGNCiHGNC:10869 ST8SIA1
MIMi601123 gene
neXtProtiNX_Q92185

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 29CytoplasmicSequence analysisAdd BLAST29
Transmembranei30 – 48Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST19
Topological domaini49 – 356LumenalSequence analysisAdd BLAST308

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi6489
OpenTargetsiENSG00000111728
PharmGKBiPA35770

Polymorphism and mutation databases

BioMutaiST8SIA1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001492821 – 356Alpha-N-acetylneuraminide alpha-2,8-sialyltransferaseAdd BLAST356

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi71N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi119N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi138 ↔ 287By similarity
Disulfide bondi152 ↔ 347By similarity
Glycosylationi214N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi245N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ92185
PeptideAtlasiQ92185
PRIDEiQ92185

PTM databases

iPTMnetiQ92185
PhosphoSitePlusiQ92185

Expressioni

Tissue specificityi

Strongly expressed in melanoma cell lines, adult and fetal brain and to a lesser extent in adult and fetal lung.2 Publications

Gene expression databases

BgeeiENSG00000111728
CleanExiHS_ST8SIA1
ExpressionAtlasiQ92185 baseline and differential
GenevisibleiQ92185 HS

Organism-specific databases

HPAiHPA026775

Interactioni

Protein-protein interaction databases

BioGridi11238010 interactors.
STRINGi9606.ENSP00000379353

Structurei

3D structure databases

ProteinModelPortaliQ92185
SMRiQ92185
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni188 – 190Substrate bindingBy similarity3
Regioni274 – 276Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692 Eukaryota
ENOG410XT8P LUCA
GeneTreeiENSGT00550000074407
HOGENOMiHOG000090201
HOVERGENiHBG106106
InParanoidiQ92185
KOiK03371
OMAiVYNHSYI
OrthoDBiEOG091G0CER
PhylomeDBiQ92185
TreeFamiTF323961

Family and domain databases

Gene3Di3.90.1480.201 hit
InterProiView protein in InterPro
IPR001675 Glyco_trans_29
IPR038578 GT29-like_sf
IPR012163 Sialyl_trans
PfamiView protein in Pfam
PF00777 Glyco_transf_29, 1 hit
PIRSFiPIRSF005557 Sialyl_trans, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q92185-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSPCGRARRQ TSRGAMAVLA WKFPRTRLPM GASALCVVVL CWLYIFPVYR
60 70 80 90 100
LPNEKEIVQG VLQQGTAWRR NQTAARAFRK QMEDCCDPAH LFAMTKMNSP
110 120 130 140 150
MGKSMWYDGE FLYSFTIDNS TYSLFPQATP FQLPLKKCAV VGNGGILKKS
160 170 180 190 200
GCGRQIDEAN FVMRCNLPPL SSEYTKDVGS KSQLVTANPS IIRQRFQNLL
210 220 230 240 250
WSRKTFVDNM KIYNHSYIYM PAFSMKTGTE PSLRVYYTLS DVGANQTVLF
260 270 280 290 300
ANPNFLRSIG KFWKSRGIHA KRLSTGLFLV SAALGLCEEV AIYGFWPFSV
310 320 330 340 350
NMHEQPISHH YYDNVLPFSG FHAMPEEFLQ LWYLHKIGAL RMQLDPCEDT

SLQPTS
Length:356
Mass (Da):40,519
Last modified:February 1, 1997 - v1
Checksum:i452FE04856964395
GO
Isoform 2 (identifier: Q92185-2) [UniParc]FASTAAdd to basket
Also known as: Sat-2

The sequence of this isoform differs from the canonical sequence as follows:
     128-135: ATPFQLPL → MQSPSFVK
     136-356: Missing.

Show »
Length:135
Mass (Da):15,504
Checksum:iC6807FD99973BDDC
GO

Sequence cautioni

The sequence AAC37586 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA54891 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_047583128 – 135ATPFQLPL → MQSPSFVK in isoform 2. 2 Publications8
Alternative sequenceiVSP_047584136 – 356Missing in isoform 2. 2 PublicationsAdd BLAST221

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77922 mRNA Translation: CAA54891.1 Different initiation.
D26360 mRNA Translation: BAA05391.1
L32867 mRNA Translation: AAA62366.1
L43494 mRNA Translation: AAC37586.1 Different initiation.
AY569975 mRNA Translation: AAS75783.1
EU041716 mRNA Translation: ABU62753.1
AK290941 mRNA Translation: BAF83630.1
AK315340 mRNA Translation: BAG37739.1
AC007544 Genomic DNA No translation available.
AC007671 Genomic DNA No translation available.
AC053513 Genomic DNA No translation available.
AC087318 Genomic DNA No translation available.
AC138468 Genomic DNA No translation available.
CH471094 Genomic DNA Translation: EAW96468.1
BC117285 mRNA Translation: AAI17286.1
BC126162 mRNA Translation: AAI26163.1
CCDSiCCDS8697.1 [Q92185-1]
PIRiA54032
RefSeqiNP_001291379.1, NM_001304450.1
NP_003025.1, NM_003034.3 [Q92185-1]
UniGeneiHs.408614

Genome annotation databases

EnsembliENST00000261197; ENSP00000261197; ENSG00000111728 [Q92185-2]
ENST00000396037; ENSP00000379353; ENSG00000111728 [Q92185-1]
GeneIDi6489
KEGGihsa:6489
UCSCiuc001rfo.4 human [Q92185-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSIA8A_HUMAN
AccessioniPrimary (citable) accession number: Q92185
Secondary accession number(s): A8K4H6
, Q17RL0, Q6PZN5, Q93064
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome